Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,744,454 C→T 100% G73D (GGC→GAC)  apt ← adenine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,744,4540CT99.2% 64.0 / ‑6.7 24G73D (GGC→GAC) aptadenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (13/10);  total (13/11)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACC  >  CP000730/1744318‑1744584
                                                                                                                                        |                                                                                                                                  
atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTACCAGGTGCAAAGTCaa                                                                                                                                 >  1:174414/1‑140 (MQ=255)
atcTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCac                                                                                                                                 >  2:87377/1‑139 (MQ=255)
     tAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATcccc                                                                                                                            >  1:268688/1‑140 (MQ=255)
      aaTTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCa                                                                                                                           <  1:256155/140‑1 (MQ=255)
       aTTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCAt                                                                                                                          >  1:78513/1‑140 (MQ=255)
                  ttGACCTGGTTTAAGTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTa                                                                                                               <  1:31089/140‑1 (MQ=255)
                       cttgTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGa                                                                                                          <  1:99684/137‑1 (MQ=255)
                          gTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAg                                                                                                       >  2:197860/1‑140 (MQ=255)
                                           tgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCaac                                                                                      >  1:283726/1‑138 (MQ=255)
                                                    aaaaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAgg                                                                             >  1:55018/1‑140 (MQ=255)
                                                           ttGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGGTTCAGGTCCTACa                                                                      >  2:117434/1‑140 (MQ=255)
                                                               aCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGa                                                                  >  1:270087/1‑140 (MQ=255)
                                                               aCCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCAAGCGATTCAGGTACTACAACGa                                                                  >  2:258400/1‑140 (MQ=255)
                                                                             cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTg                                                    <  1:252755/140‑1 (MQ=255)
                                                                               tACTCATAACGAATGACTTCACGAGGTAAGTTCCCTTCTTTTCTAACAGGTGCGAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCGATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACTTCTCTGTc                                                  <  2:235589/140‑1 (MQ=255)
                                                                                  ccATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCAATTGAATAAGCTACACGACAGCCAATGATCAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt                                               <  1:117434/139‑1 (MQ=255)
                                                                                           aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCCCTGTCTTTTGCTTATTc                                      >  1:83087/1‑140 (MQ=255)
                                                                                                  cACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTAAAAtt                               >  1:3952/1‑140 (MQ=255)
                                                                                                   aCGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGTCACTCCCCATTGAATAAGCTACATGACAGCCAACGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAttt                              <  2:263989/140‑1 (MQ=255)
                                                                                                         tAATTTCCCTTCTTTTCTAGCAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTg                        <  1:90912/140‑1 (MQ=255)
                                                                                                                      tttCTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCata           <  1:288326/140‑1 (MQ=255)
                                                                                                                       tccTAACAGGTGCAAAGTCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCatat          <  1:319536/138‑1 (MQ=255)
                                                                                                                              aGGTGCAAAGTCAATCCCCACTGAATTCACTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCTTTGCCTGATGCTTctc   >  1:76771/1‑138 (MQ=255)
                                                                                                                               ggTGCAAAGCCATTCCCCCTTGACTAAGCAACCGGACAGCCAATGATAAGTCGCCGCGCTTCAGGTCCTACAACGATATCAACATCTTTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAcc  <  2:220208/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                  
ATCTGTAATTAACACACGTTGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACC  >  CP000730/1744318‑1744584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: