BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,440,007	Δ1 bp	coding (34/201 nt)	cspA ←	cold shock protein CspA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,440,007	0	C	.	97.2%	803.1 / 16.7	182	coding (34/201 nt)	cspA	cold shock protein CspA
Reads supporting (aligned to +/- strand): ref base C (1/1); major base . (79/95); minor base T (1/4); total (81/101)
Fisher's exact test for biased strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

TTGTGGACCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGC  >  CP000730/1439864‑1440150

ttGTGGACCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGCTAAAGCCGAATCCTTTTT‑AGCGtt                                                                                                                                           >  1:509661/1‑149 (MQ=255)

    ggACCGCGGTCGCCTTCAACTTCTTCAAACTTCACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAAc                                                                                                                                       <  1:204301/149‑1 (MQ=255)

    ggACCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAAc                                                                                                                                       >  2:1047311/1‑149 (MQ=255)

      aCCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAAccg                                                                                                                                     >  1:1437242/1‑148 (MQ=255)

      aCCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCa                                                                                                                                     >  2:41607/1‑149 (MQ=255)

       ccGCGGTCGCCTTCAACTACTTCAACCTCAACCGCTTGACCTTCTTCTAATGATTTGTAACCATCTTCGTTAATTGCTCAAAAATGTTCGAAGACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCAt                                                                                                                                    <  1:469990/149‑1 (MQ=255)

        cgcgGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCAtt                                                                                                                                   >  1:714508/1‑149 (MQ=255)

         gcgGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCAttt                                                                                                                                  <  1:391792/149‑1 (MQ=255)

               gCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGt                                                                                                                            <  1:1538384/149‑1 (MQ=255)

                     aaCTACTTCAAATTCAACTGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGGGTTAAACCATTTAACTGTAGCttt                                                                                                                      >  2:262628/1‑148 (MQ=255)

                     aaCTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTACCTGTACCTTg                                                                                                                      >  1:998432/1‑149 (MQ=255)

                     aaCTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCttt                                                                                                                      >  2:1670039/1‑148 (MQ=255)

                     aaCTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTg                                                                                                                      >  2:1135526/1‑149 (MQ=255)

                         actTCAAACTCAACAGCTTGACCTTGTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAACATGTACGAATACGTCCTTTTCTCCTTCAACTTCGATAAAGCCGAAGCCTTTTT‑AGCGTTAAACCATTTGAGTGTACTTTGTTTc                                                                                                                  >  1:748577/1‑149 (MQ=255)

                         actTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTc                                                                                                                  <  1:621434/149‑1 (MQ=255)

                         actTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTc                                                                                                                  <  2:941650/149‑1 (MQ=255)

                          ctTCAAACTCCACAGCTTGACCTTCTTCTAATGATTTGTACCCTTCTTGGAAAAAAAAAAAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCa                                                                                                                 <  2:153844/149‑1 (MQ=255)

                          ctTCAAACTCAACTGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCa                                                                                                                 <  1:1160833/149‑1 (MQ=255)

                            tCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTAGGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATa                                                                                                               >  1:1070966/1‑149 (MQ=255)

                            tCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATa                                                                                                               >  1:1030212/1‑149 (MQ=255)

                              aaaCTCAACAGATTAACCATCTTATACAGATTTGTTATTTTCTTCGTAATTATATGAATAATGAGCAGATTCTCCATTTGCGCCTTCAACTTCTATAAAGTCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAAt                                                                                                             <  2:1666970/149‑1 (MQ=255)

                                aCTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCGTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATTATCt                                                                                                           >  2:1489436/1‑149 (MQ=255)

                                    aaCAGCTTGACCTTCTTCTAATGATTTGTAACCCTCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGaaa                                                                                                       <  1:666932/149‑1 (MQ=255)

                                      cAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAAcc                                                                                                     >  2:1632925/1‑149 (MQ=255)

                                          ttGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTCAAACCTCCa                                                                                                 >  1:1849468/1‑149 (MQ=255)

                                            tgccTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAg                                                                                               <  2:455879/147‑1 (MQ=255)

                                             cccTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGa                                                                                              <  1:1710090/148‑1 (MQ=255)

                                             aCCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCaccc                                                                                              <  2:1334311/149‑4 (MQ=255)

                                              ccTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGAc                                                                                             >  2:481419/1‑149 (MQ=255)

                                              ccTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGAc                                                                                             <  1:1663939/149‑1 (MQ=255)

                                                     ttAATGATTTGTAACCTTCTTGGTTAATGTCTTAAAAATGTACGAATACGTCATTTTCTCCTTCTACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAatt                                                                                      <  2:1445771/148‑1 (MQ=255)

                                                       aaTGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAattca                                                                                    <  1:1905455/149‑1 (MQ=255)

                                                       aaTGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAattca                                                                                    <  1:1007078/149‑1 (MQ=255)

                                                        aTGATTTGTACCCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTAGATAAAGCCGACTACTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAattcat                                                                                   <  1:869645/149‑1 (MQ=255)

                                                         tGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAattcatt                                                                                  >  1:180303/1‑149 (MQ=255)

                                                         tGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTAAACGTCGATAAAGCCGAAACCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATGTGAAACCTCCAAGACTAAAattcatt                                                                                  >  1:666225/1‑149 (MQ=255)

                                                            tttGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCaa                                                                               <  1:362381/149‑1 (MQ=255)

                                                            tttGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCaa                                                                               >  2:902255/1‑149 (MQ=255)

                                                                tAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATg                                                                           <  1:41607/149‑1 (MQ=255)

                                                                 aaCCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTCAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGc                                                                          <  1:1050990/149‑1 (MQ=255)

                                                                   ccATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCtt                                                                        <  2:655562/149‑1 (MQ=255)

                                                                        ttGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCg                                                                   <  1:803464/149‑1 (MQ=255)

                                                                        ttGGTTAATCGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTGCCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCg                                                                   <  1:609158/149‑1 (MQ=255)

                                                                         ttgTTAATTGCCGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGc                                                                  <  2:118988/147‑1 (MQ=255)

                                                                         tGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTATACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGc                                                                  <  1:274145/149‑1 (MQ=255)

                                                                         tGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGc                                                                  <  1:182881/149‑1 (MQ=255)

                                                                         tGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGc                                                                  <  1:1736025/149‑1 (MQ=255)

                                                                          ggTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCa                                                                 <  2:845968/149‑1 (MQ=255)

                                                                           gTTAATTCCTGAAAAATGTACTAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCat                                                                <  2:3781/149‑1 (MQ=255)

                                                                             tAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCatat                                                              <  2:762180/149‑1 (MQ=255)

                                                                              aaTTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTCACTGATTTTTCTGCAATATCTGTCATAATATACCCGCTGCAAGACTAAAATTCATTCACTCTGCTTATTCGCATAtt                                                             <  2:1411190/149‑1 (MQ=255)

                                                                                   cTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAGGACTACAATTAATTCATGCTGCTTATTAGCATGTTACaaa                                                        >  2:877737/1‑149 (MQ=255)

                                                                                   cTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACaaa                                                        <  1:168509/149‑1 (MQ=255)

                                                                                   cTGAAAAATGTAAGCATACGTCCTTTTCTCTTTAAACGTCGATCAAGCCGATTCCTTTTT‑AGCGTTAAACCATTTAACGGTACCTTGCTTCATAATCTGAAACCTACAAGACTAAAATTCATTCAATATGCTTATTCGCATATATCaaa                                                        >  2:772950/1‑149 (MQ=255)

                                                                                       aaaaTGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGGAACCTCCAAGACTAAAATTCATTCAATGTTCTTATTCTCGTAGTACaaaaaaa                                                    >  1:1403904/1‑148 (MQ=255)

                                                                                       aaaaTGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAAt                                                    >  2:1807695/1‑149 (MQ=255)

                                                                                       aaaaTGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAAt                                                    >  1:231423/1‑149 (MQ=255)

                                                                                        aaaTGTACGATTACGTCATGTTCCCCTTCACCTTCGGTAAAGTGGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATa                                                   <  1:857820/149‑1 (MQ=255)

                                                                                         aaTGTACGAATACGTCATTTCCTCCTTCGACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAATCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATAc                                                  <  1:1135734/149‑1 (MQ=255)

                                                                                          aTGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAGCCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATGTTACAAAAAAAACa                                                 >  1:1343485/1‑149 (MQ=255)

                                                                                          aTGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTCACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACa                                                 <  2:1715201/149‑1 (MQ=255)

                                                                                            gTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATg                                               >  1:1921170/1‑149 (MQ=255)

                                                                                             tACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGc                                              >  1:1335875/1‑149 (MQ=255)

                                                                                             tACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCAAATCCATTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATGCACCTAGTACaaaaaaagactta                                              >  2:823511/1‑142 (MQ=255)

                                                                                             tACGAATACGTCATTTTCTCCTTCAACTGCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTCAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATgt                                              >  1:1405644/1‑148 (MQ=255)

                                                                                              accaaTACGTCATTTTTTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAATCCTCCAAGACTACAATTCATTCAATATGCTTATTGGCATATTACAAAAAATACATGCa                                             <  1:285936/146‑1 (MQ=255)

                                                                                                 aaTACGTCATTTTCTCCTGCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAAt                                          <  2:487355/149‑1 (MQ=255)

                                                                                                     cGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGACACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCa                                      <  2:1894112/149‑1 (MQ=255)

                                                                                                     cGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAGATTTCa                                      >  1:146815/1‑149 (MQ=255)

                                                                                                     cGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCa                                      >  1:1090981/1‑149 (MQ=255)

                                                                                                     cGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCa                                      <  1:1244890/149‑1 (MQ=255)

                                                                                                      gTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCAc                                     <  1:1631714/149‑1 (MQ=255)

                                                                                                      gTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCAc                                     <  1:1598149/149‑1 (MQ=255)

                                                                                                      gTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCAc                                     >  2:681522/1‑149 (MQ=255)

                                                                                                      gTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCAc                                     >  2:1351729/1‑149 (MQ=255)

                                                                                                       tCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACt                                    >  2:1929908/1‑149 (MQ=255)

                                                                                                       tCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACt                                    >  2:512398/1‑149 (MQ=255)

                                                                                                       tCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACt                                    <  1:1750332/149‑1 (MQ=255)

                                                                                                           tttCTCCTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCaaa                                <  1:1380309/149‑1 (MQ=255)

                                                                                                               cacTTCAACGTCGACAAAGCCTAAT‑CTTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGAATAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATAtt                            <  2:393149/147‑1 (MQ=255)

                                                                                                                ccTTCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTc                           >  2:578111/1‑149 (MQ=255)

                                                                                                                  ttCAACTTCGATAAAGCCGAATCCTTTTT‑GGCTTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCtt                         >  1:970496/1‑149 (MQ=255)

                                                                                                                  ttCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCtt                         >  1:24022/1‑149 (MQ=255)

                                                                                                                  ttCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCtt                         >  1:533428/1‑149 (MQ=255)

                                                                                                                   tCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCttt                        <  1:910149/149‑1 (MQ=255)

                                                                                                                   tCAACTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTCCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCttt                        >  2:330161/1‑149 (MQ=255)

                                                                                                                      aCTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACCCAAAACACAGGCAAATTTCACTCAAATATTCTTTGCa                     <  1:481419/149‑1 (MQ=255)

                                                                                                                      aCTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCa                     <  1:697795/149‑1 (MQ=255)

                                                                                                                      aCTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCa                     >  1:763877/1‑149 (MQ=255)

                                                                                                                      aCTTCGATAAAGCCGAATCCTTTGT‑AGCGTTAAACCATTGAACTGTACCTTCTTTCATAATCTGACACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCTCTCAAATATTCTTTGCa                     <  2:1093160/149‑1 (MQ=255)

                                                                                                                       cTTCGATAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAACCCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCaa                    <  2:1013978/149‑1 (MQ=255)

                                                                                                                       cTTCGACAAAGCCCAATCC‑TGTTCAGCGTTAAACCAGTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATCCAACTTTCACTCAAatacctgttatag                    <  2:1838908/149‑11 (MQ=255)

                                                                                                                            aTAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGAATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATgg               <  2:714508/149‑1 (MQ=255)

                                                                                                                             tAAAGCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTCTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATCTGCTTATTCGCATATTACAAAAAATACATGCAAGTTTCACTCAACGATTCTTTGCAATATGGa              >  1:1608898/1‑149 (MQ=255)

                                                                                                                               aaGCCGAATCCTTTTT‑AGCGTTAAACCATTTAAATGTACATTGTTTCATAATCTGAAACCACCAAGACTAAAAGTCATTCAATATGCTTATACGCATATTACAAAAAATACTTGCAAATTTCACTCAAATATTCTTTGCAATATGAaat            >  2:95024/1‑149 (MQ=255)

                                                                                                                                 gCCGAATCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAATTATTCTTTGCAATATGGaataa          >  2:462967/1‑149 (MQ=255)

                                                                                                                                     aaTCCTTTTT‑AGCGTTAAACCATTTCACTGTACCTTGTTTCATTATCTGACACCTCCAAGACTAAAATTAATTCATTATGCTTATTCGCATCTTACAAAAAATACATGCAAATTTCACTCAAATTTTCTTTGCCATATGGaatgctgct      >  1:315184/1‑143 (MQ=255)

                                                                                                                                     aaTCCTTTTT‑AGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAatcat      >  2:493098/1‑149 (MQ=255)

                                                                                                                                         ccttttTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCtc   >  2:814786/3‑148 (MQ=255)

                                                                                                                                          cttttTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAACTATCCTTTGCAATATGGAATAATCATCTGc  <  2:18746/148‑1 (MQ=255)

TTGTGGACCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGC  >  CP000730/1439864‑1440150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`