Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 36,378 C→T L58L (TTG→TTA)  tpn ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,3780CT93.3% 7020.2 / inf 3366L58L (TTG→TTA) tpnIS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (110/115);  new base T (1572/1543);  total (1702/1664)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3368 total aligned reads displayed.

GTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAA  >  CP000730/36230‑36526
                                                                                                                                                     |                                                                                                                                                    
     ttACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTAATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAAt                                                                                                                                                 >  2:1898993/1‑149 (MQ=255)
       aCGCAACCAATTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTg                                                                                                                                               <  1:254880/149‑1 (MQ=255)
       aCGCAACCAAATATCTAATGTATGTCCCTCTGTATCGATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTCTAATAAGCTTTTTTATGCTTTCTCT‑TCCAAATTTGATATAAAATTg                                                                                                                                               <  1:1111278/149‑1 (MQ=11)
       aCGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTg                                                                                                                                               <  1:647721/149‑1 (MQ=37)
        cGCAACCAAATATCTAATGTATGTTCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTgg                                                                                                                                              <  2:253092/149‑1 (MQ=255)
         gCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTTTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCGAATTTGTTATAAACTTggg                                                                                                                                             >  1:988144/1‑149 (MQ=11)
          cAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTgggg                                                                                                                                            <  1:786575/149‑1 (MQ=37)
            aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCa                                                                                                                                          <  1:1828381/149‑1 (MQ=37)
             ccAAATATCTAATTTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCat                                                                                                                                         <  2:795233/149‑1 (MQ=255)
                aatatacaaTGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATAtt                                                                                                                                      <  2:973320/142‑1 (MQ=11)
                aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTATTAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGAATAtt                                                                                                                                      >  1:918970/1‑149 (MQ=11)
                 atatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAAGTTGATATAAAATTGGGGCATATTc                                                                                                                                     >  1:1225414/1‑149 (MQ=255)
                  tatCTAATGTTTGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATCTGCTATAAAATTGTGGCATATTCt                                                                                                                                    <  2:677746/149‑1 (MQ=11)
                  tatCTAATGTATGTCCCTCTGTATCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCt                                                                                                                                    <  1:1157317/149‑1 (MQ=255)
                       atggtagttCCCTCTGTATCAATGGCAAGACATTAATATCCCCACTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                                                                                               <  1:1542800/141‑1 (MQ=11)
                        atgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAAcc                                                                                                                              >  2:155487/1‑149 (MQ=255)
                        atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTTAAcc                                                                                                                              >  2:1392004/1‑149 (MQ=255)
                         tgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGGCTTTTCTTGAAccc                                                                                                                             >  1:679771/1‑149 (MQ=11)
                           tatgtCCCTCTGCATCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCaa                                                                                                                           >  2:1731418/1‑149 (MQ=255)
                              gtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                                                                                        >  1:316096/1‑149 (MQ=255)
                              gtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTCGGGCATATTCTTTAACCCAACgg                                                                                                                        >  2:1699142/1‑149 (MQ=11)
                               tcgctctGTATCTATGTCACGATATAAATAGCGCCATTTTCCTTTTATTTTGATATACGTCGCATCAATACGCCATTTGTAATAAGCTTGTTTATGATTTTTTTATACAAGTTTTA‑AAAAAGTTGGGGCATATTCTTGAACCCacaggt                                                                                                                       >  2:904419/4‑144 (MQ=2)
                               tCCCTCTGTATCAATGGCACGATAGAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCTAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                                       >  1:1186167/1‑149 (MQ=11)
                               tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                                       >  2:1759604/1‑149 (MQ=40)
                               tCCCGCTGTATCAATGGCACGATATAAATGGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                                       <  2:1236188/149‑1 (MQ=11)
                                cccTCTACATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTa                                                                                                                      >  2:1154623/1‑149 (MQ=19)
                                   tctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATCTAAAATTGGGGCTTATTCTTTCACCCAAGGGTAgtt                                                                                                                   >  2:127118/1‑147 (MQ=11)
                                   tctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGat                                                                                                                   <  1:287345/149‑2 (MQ=255)
                                     tgtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCg                                                                                                                 <  1:444764/146‑1 (MQ=255)
                                      gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                                <  1:1677448/147‑1 (MQ=255)
                                         tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                             >  2:1427637/1‑149 (MQ=25)
                                         tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                             <  1:1751255/149‑1 (MQ=255)
                                          cactGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTCCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGaa                                                                                                            <  1:1906720/146‑1 (MQ=255)
                                          cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGaa                                                                                                            >  1:1354371/1‑149 (MQ=25)
                                          cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTgggg                                                                                                                                            <  2:357510/117‑1 (MQ=255)
                                          cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                            >  2:1349373/1‑149 (MQ=255)
                                          cAATGGCACGATACAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCTATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGaa                                                                                                            <  1:586472/149‑1 (MQ=255)
                                               gCACGATATAAATAGCTCCCTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                                                                                                       <  1:1646807/149‑1 (MQ=255)
                                                cACGACATAAATAGCTCCATCTTCCCTTCGTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                                      <  2:641620/149‑1 (MQ=11)
                                                 aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGaa                                                                                                     <  1:1983583/149‑1 (MQ=40)
                                                 aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                                                                                                     <  1:1939603/149‑1 (MQ=255)
                                                  cGATATAAATAGCTCCATTTTCCTTTTATTTTGCAGAACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGAATATTCTTGAACCCACAGGTAGACCGTTGAATGGTGAAc                                                                                                    >  2:430990/1‑149 (MQ=11)
                                                    ttataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                                                                                                  <  2:1061949/148‑1 (MQ=255)
                                                    atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                                                                                                  <  2:1195153/149‑1 (MQ=255)
                                                    atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                                                                                                  <  2:939308/149‑1 (MQ=255)
                                                      ataAATAGCCCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACTCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGttt                                                                                                <  2:469027/149‑1 (MQ=11)
                                                            aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                                                                                          <  1:1576610/149‑1 (MQ=255)
                                                            aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAAGGTAGATCGTTGAATGATGAACGTTTAcacca                                                                                          <  2:722074/149‑1 (MQ=255)
                                                              cTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                                                                                        >  1:1002639/1‑149 (MQ=25)
                                                              cTCCATTTTCCTTTTATCTTGATGTACGTCTCATCAATACGCCATTTGTAATCAGCTATTGTTGGCTTTTTCT‑TCACACTCTGATACAAAATTGGGGCATATTCTGTAACCCAACATTATACCGTTGAATAATGAACGGTTACACCACg                                                                                        >  1:1463478/1‑149 (MQ=11)
                                                               tCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGt                                                                                       >  2:5500/1‑149 (MQ=25)
                                                               tCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                                       >  1:215341/1‑149 (MQ=25)
                                                               tCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                                       >  1:12529/1‑149 (MQ=21)
                                                                ccATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt                                                                                      >  2:518258/1‑149 (MQ=21)
                                                                ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                                      >  2:186653/1‑149 (MQ=255)
                                                                 cATTTTCCTTTTATTTTGATCTACGCCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTc                                                                                     <  2:1100733/149‑1 (MQ=255)
                                                                 cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAACTTTGATATAAAATTGGGGCCTATTCTTGAACCCCATGGCCGATCGTTGAATGATGAACGtggtgtaaacgtcc                                                                                     <  1:1389056/149‑14 (MQ=11)
                                                                  cttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTcc                                                                                    <  2:1829804/148‑1 (MQ=21)
                                                                  cttgtCCTTTTATTTTGATGGTCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGCTACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTcc                                                                                    <  2:326610/145‑1 (MQ=255)
                                                                   ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctc                                                                                   <  1:553530/149‑3 (MQ=255)
                                                                    tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGTTTTTTCC‑GCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                                  <  1:952764/149‑1 (MQ=255)
                                                                    tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                                  <  2:1976643/149‑1 (MQ=21)
                                                                      tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTa                                                                                <  2:2113/149‑1 (MQ=21)
                                                                      tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTa                                                                                <  2:864558/149‑1 (MQ=21)
                                                                      tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                                <  1:386817/149‑1 (MQ=11)
                                                                        cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAat                                                                              <  1:1792617/149‑1 (MQ=21)
                                                                          tttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                            >  1:891752/1‑149 (MQ=21)
                                                                          tttATTTTGATATAAGGCTCATCAATACGCCATTTGTAATAAGATTTTTTATGCTTTTTCT‑TCCAAGATTGGTATAAAATTGGGGCATATTCGTGAACCCAACGGTAGCTCGTTGAATGATGAATGTTTAGACCACGTTCTCTTTatat                                                                            >  2:1533081/1‑149 (MQ=11)
                                                                             aTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTACTATTTc                                                                         >  1:616385/1‑149 (MQ=25)
                                                                             aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTc                                                                         <  2:1498413/149‑1 (MQ=255)
                                                                               tttGCTATACGTCTCATCAATACGCCATTTGTAATAAGATTTGTGATTCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                       <  1:1037661/149‑1 (MQ=11)
                                                                               tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAGCCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                       <  1:34720/149‑1 (MQ=11)
                                                                               tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                       >  2:1585696/1‑149 (MQ=255)
                                                                                ttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                                                                                                <  2:1321861/91‑1 (MQ=255)
                                                                                ttGATATACGGCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                                      <  1:1509482/149‑1 (MQ=11)
                                                                                 tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACATTCTCTTAATATTTCAGat                                                                     <  2:754991/149‑1 (MQ=11)
                                                                                  gATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAATGTTTACACTACGTTTTCTTAATATTTCAGata                                                                    >  2:597621/1‑149 (MQ=11)
                                                                                  gATATACGTCTCATCAATACGCCATTTGAAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGata                                                                    <  2:1795376/149‑1 (MQ=11)
                                                                                   atatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatat                                                                   >  2:1662974/4‑149 (MQ=255)
                                                                                    tatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatata                                                                  >  2:298364/3‑149 (MQ=255)
                                                                                    tGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatata                                                                  >  2:39154/1‑149 (MQ=25)
                                                                                       aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                               >  2:840360/1‑149 (MQ=255)
                                                                                       aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                               >  1:753651/1‑149 (MQ=25)
                                                                                        cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACGACGTTCTCTTAATATTTCAGATATATCAc                                                              >  1:483217/1‑149 (MQ=255)
                                                                                        cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                              >  1:1289619/1‑149 (MQ=255)
                                                                                        cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                              >  2:1020605/1‑149 (MQ=255)
                                                                                           ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                           >  1:1319846/1‑149 (MQ=255)
                                                                                           ctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGAt                                                           >  1:1613855/1‑149 (MQ=40)
                                                                                               tcaATCCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                       <  1:186864/149‑1 (MQ=18)
                                                                                               tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAGGTTAACGCCAAGTTCACTTAATTTTTTAGATACATAAAGATattc                                                       >  1:414986/1‑146 (MQ=11)
                                                                                               tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                       >  1:106679/1‑149 (MQ=255)
                                                                                               tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                       <  2:1629479/149‑1 (MQ=11)
                                                                                               tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                       <  1:1427637/149‑1 (MQ=18)
                                                                                                  aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                                    <  1:830013/149‑1 (MQ=18)
                                                                                                   tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                   <  1:1709407/149‑1 (MQ=11)
                                                                                                   tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                   <  1:862125/149‑1 (MQ=11)
                                                                                                    aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                  >  1:513334/1‑149 (MQ=21)
                                                                                                    aCGCCACTTGTATTAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                  <  1:719416/149‑1 (MQ=255)
                                                                                                     cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAACATTGCAGATATATCACGATAGCTCAATgc                                                 <  2:1935692/149‑2 (MQ=11)
                                                                                                           ttGTAATAAGCTTTTTTATGTTTTTTCT‑TCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATGCATATCt                                           <  2:1467583/149‑1 (MQ=11)
                                                                                                                                                     |                                                                                                                                                    
GTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCT‑TCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAA  >  CP000730/36230‑36526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑