Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,621,781 A→C L297* (TTA→TGA)  USA300HOU_2485 ← MFS family major facilitator transporter, cation symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,621,7810AC98.8% 583.1 / ‑5.5 172L297* (TTA→TGA) USA300HOU_2485MFS family major facilitator transporter, cation symporter
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (86/84);  total (87/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 173 total aligned reads displayed.

TACTAAATACTTTCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATAAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCTTTTAA  >  CP000730/2621635‑2621926
                                                                                                                                                  |                                                                                                                                                 
    aaaTACTTTCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCaa                                                                                                                                             >  1:1065081/1‑149 (MQ=255)
     aaTACTTTCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGCCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGACACACATTAATAATCGATCAGCCCAt                                                                                                                                            >  1:145951/1‑149 (MQ=255)
       tccTTTCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAACGTTGCACCTATATATACTATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTc                                                                                                                                          <  1:58959/147‑1 (MQ=255)
       tACTTTCCCCATCAAATGATCTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTc                                                                                                                                          <  1:1108156/149‑1 (MQ=255)
        aCTTTCCCCATCAGATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTcc                                                                                                                                         <  2:295508/149‑1 (MQ=255)
         cTTTCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTccc                                                                                                                                        >  1:1610778/1‑149 (MQ=255)
            tCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACCTTAATAATCGATCAGCCAATTCCCCaa                                                                                                                                     <  1:1491834/149‑1 (MQ=255)
             ccccATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAATCCCTCTATAGATTGTCCAAGACCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAAACGATCAGCCAATTCCCCaaa                                                                                                                                    >  2:1174547/1‑149 (MQ=255)
                  tCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTaaa                                                                                                                               <  2:1187504/149‑1 (MQ=255)
                  tCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAAGAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCACTTCCCCAAACTaaa                                                                                                                               <  1:798231/149‑1 (MQ=255)
                     aaTGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCATACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAaca                                                                                                                            <  1:1370055/149‑1 (MQ=255)
                       tGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAGTTCCCCAAACTAAAACAcc                                                                                                                          <  2:883929/149‑1 (MQ=255)
                             ggCTTTTTTATTTGAAGGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCACGTCCCAATAATGTTGCACCTATATATAATCTCGCCACCCCTGGAAACATACTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACt                                                                                                                    <  2:331453/149‑1 (MQ=255)
                              gCGTTTTTATTTGAATGGCAACTGGTTTTGTTAGTCCCGCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTa                                                                                                                   <  2:257317/149‑1 (MQ=255)
                              gCGTTTTGATTTGAATGGCAACTGATCTTGTTAGTCCCTCCATAGATTGTCCAAGTCCCTATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATCCCCCAAACTAAAACACCAATACTa                                                                                                                   <  2:1588702/149‑1 (MQ=255)
                                        ttGAATGGCAACTGAGCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAACAATATTTCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGa                                                                                                         <  2:1204066/149‑1 (MQ=255)
                                         tGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGAt                                                                                                        >  2:1510496/1‑149 (MQ=255)
                                              ggCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATc                                                                                                   >  1:316417/1‑149 (MQ=255)
                                                            ttAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGc                                                                                     >  2:683056/1‑149 (MQ=255)
                                                              aGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATACCGCCACACTTGGAAACACATTAATAAGCGATCAGCCAAGTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCaa                                                                                   <  1:1065004/149‑1 (MQ=255)
                                                                  ccTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAAccc                                                                               <  1:412470/149‑1 (MQ=255)
                                                                   ctctATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCa                                                                              >  1:286765/1‑149 (MQ=255)
                                                                    cctATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAACCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCAt                                                                             <  2:906897/148‑1 (MQ=255)
                                                                     ctATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCACACTACCACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATa                                                                            <  1:1562809/149‑1 (MQ=255)
                                                                        taGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATc                                                                         <  1:1048314/149‑1 (MQ=255)
                                                                         aGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACTCCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCa                                                                        <  2:1065081/149‑1 (MQ=255)
                                                                         aGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCa                                                                        <  2:695853/149‑1 (MQ=255)
                                                                         aGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCa                                                                        >  1:1619725/1‑149 (MQ=255)
                                                                          gagtggcccaGTCCCAACAATGTTCCGAGTAGATATACTCTCGCCACAGTGGGAAGGAGATGGTGGATCGATCAGCCAATGCCCGAAGCTAACACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCaa                                                                       <  2:1818168/140‑1 (MQ=255)
                                                                          gATTGTCCAAGTCCCAATAATTTTGCACCTATATATAATATCGCCAGAGTTGTAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTTACAGCAAACTCATAATCaa                                                                       <  2:560080/149‑1 (MQ=255)
                                                                            ttGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATg                                                                     >  1:1826123/1‑149 (MQ=255)
                                                                               tCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTACCACACCAAGACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACa                                                                  <  1:1885303/149‑1 (MQ=255)
                                                                               tCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACa                                                                  <  1:914823/149‑1 (MQ=255)
                                                                               cccagcgcccAGTAATGTTACACCTATATATAATACCCCCACACTTGGAAACACATTAATAATCTATCAGCCAATTCCCCAAACTACAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACa                                                                  <  2:206289/142‑1 (MQ=255)
                                                                                ccAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACAt                                                                 >  1:1029909/1‑149 (MQ=255)
                                                                                  aaGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCACACCCATAATCAATGACATta                                                               >  1:1952373/1‑149 (MQ=255)
                                                                                   aGTCCCTATAATGTTGCAACTAAATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATtaa                                                              >  2:1355557/1‑149 (MQ=255)
                                                                                   aGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATtaa                                                              >  2:1670595/1‑149 (MQ=255)
                                                                                   aGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATtaa                                                              >  1:804898/1‑149 (MQ=255)
                                                                                   aGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATtaa                                                              <  2:1784115/149‑1 (MQ=255)
                                                                                    gtgccaatcagcttGCATCTATATATAATTCCGCTACACTCGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAACACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATtaat                                                             <  2:1549972/137‑1 (MQ=255)
                                                                                      cccAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATtaataa                                                           <  2:383971/149‑1 (MQ=255)
                                                                                       ccAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATaaa                                                          >  2:1728261/1‑149 (MQ=255)
                                                                                        cAATAATGTTGCACCTATATATAATATCGCCACCCTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAg                                                         >  2:1417243/1‑149 (MQ=255)
                                                                                          ataatGTTGCACCTATCTATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCACTGACATtaataacacc                                                       >  2:45416/1‑145 (MQ=255)
                                                                                          ataatGTTGCACATATATATAATAGCGTCACACTTGGAAACACATTAATAATCGATAAGACAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGTTTAATCGCATTTATGACAGCAAACCCGTAATCAATGTCATTAATaaacag                                                       >  2:731860/1‑146 (MQ=255)
                                                                                           taatGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCAGAATCAATGACATTAATAAAGATg                                                      <  1:1486057/149‑1 (MQ=255)
                                                                                           aaatGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATg                                                      <  1:1183143/148‑1 (MQ=255)
                                                                                           aaatGTTCCACCTATATCAACTATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAAGCAATGACATTAATAAAGATg                                                      <  2:1911093/148‑1 (MQ=255)
                                                                                            aatGTTGCACCGATCTATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGc                                                     <  2:641588/149‑1 (MQ=255)
                                                                                              tGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCta                                                   >  1:2005362/1‑149 (MQ=255)
                                                                                                    aCCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCGAATTCCCCAAGCTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTTTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTa                                             <  2:201544/149‑1 (MQ=255)
                                                                                                      cTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTaaa                                           <  2:211911/149‑1 (MQ=255)
                                                                                                          atataATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAGGATGCTATACCTAAACatg                                       >  1:489063/1‑149 (MQ=255)
                                                                                                           tataATATCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACatga                                      <  1:1432353/149‑1 (MQ=255)
                                                                                                             taataTCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTACAACACCAATACTACATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCCATGACATTAATAAAGATGGTATTCCTAAAAataatg                                    >  1:1793995/1‑149 (MQ=255)
                                                                                                             taataTCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGCCAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACatgatg                                    >  1:1530185/1‑149 (MQ=255)
                                                                                                             taataTCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAAAACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACatgatg                                    >  1:171460/1‑149 (MQ=255)
                                                                                                                tatCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATACAGATACTATACCTAAACATGATGTAg                                 >  2:1263699/1‑149 (MQ=255)
                                                                                                                tatCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAg                                 >  1:323725/1‑149 (MQ=255)
                                                                                                                tatCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAg                                 >  2:1264321/1‑149 (MQ=255)
                                                                                                                 atCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGc                                >  2:984257/1‑149 (MQ=255)
                                                                                                                 atCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGc                                >  1:893794/1‑149 (MQ=255)
                                                                                                                  tCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCGAGGACTAATTCAAGGACCTTAATAAAGATGCTATACCTAAACATGATGTAGCt                               >  1:1959540/1‑149 (MQ=255)
                                                                                                                  tCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAACCATGATGTAGCt                               >  2:1971792/1‑149 (MQ=255)
                                                                                                                  tCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGcc                               >  1:891131/1‑148 (MQ=255)
                                                                                                                  tCGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCt                               >  2:595823/1‑149 (MQ=255)
                                                                                                                   cGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGCTTAATCGCTATTCTGACAGCCACATGAGCAGCAATGACCATAACAAAGATGCAATACCTAAACATGATGTAGCTa                              >  1:454377/1‑149 (MQ=255)
                                                                                                                   cGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATACTCAATGACATTAATAAAGATGCTATACCTCAACATGATGtcagta                              >  1:21536/1‑144 (MQ=255)
                                                                                                                   cGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTa                              >  1:175970/1‑149 (MQ=255)
                                                                                                                   cGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTa                              >  2:469610/1‑149 (MQ=255)
                                                                                                                   cGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATAATGTAGCTa                              >  1:1394646/1‑149 (MQ=255)
                                                                                                                   cGCCACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTAGACCGAACCATGATGGAGCTa                              >  1:1858129/1‑149 (MQ=255)
                                                                                                                     ccACACTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGt                            >  2:1460593/1‑149 (MQ=255)
                                                                                                                      cacaCTTTGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTc                           <  1:1331764/149‑1 (MQ=255)
                                                                                                                        cccTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCa                         <  2:1024782/147‑1 (MQ=255)
                                                                                                                        cacTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCa                         <  2:1219193/149‑1 (MQ=255)
                                                                                                                         acTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCat                        >  1:1280598/1‑149 (MQ=255)
                                                                                                                          cTTGGAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGAAGCTAGTcacaa                       >  2:1718032/1‑145 (MQ=255)
                                                                                                                             ggAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATac                    >  2:46313/1‑149 (MQ=255)
                                                                                                                              gAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCACACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCAATCTaca                   >  2:253258/1‑149 (MQ=255)
                                                                                                                              gAAACACATTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACAAAATTTAAAGATCAATCGCCTCTCTGACAGCAAACCCATAATCAATGACATCAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATacc                   >  2:1162496/1‑148 (MQ=255)
                                                                                                                               aaaCACATTAATAACCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAACCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATacac                  <  1:565240/149‑1 (MQ=255)
                                                                                                                                    caTTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACg             >  1:601168/1‑149 (MQ=255)
                                                                                                                                    caTTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGGCCATATACACCAACg             >  1:663759/1‑149 (MQ=255)
                                                                                                                                    caTTAATAATCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATGAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACg             >  2:1475024/1‑149 (MQ=255)
                                                                                                                                       taataaTCGACCAGCCAATTCCACAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGccc          <  2:947614/149‑1 (MQ=255)
                                                                                                                                         ataatCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCctc        >  1:586778/1‑149 (MQ=255)
                                                                                                                                         ataatCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCAATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGATAGTCCATATCCACCAACGCCctc        >  2:15356/1‑149 (MQ=255)
                                                                                                                                          aaatCGACCAGCCAATTCTCCAAACAAAAACACCAATACTAAATTTAAAGATTAATCGCTTTACTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCctct       <  1:203016/148‑1 (MQ=255)
                                                                                                                                            atCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTGAACATGATGTAGCTAGTCCATATACACCAACGCCCTCttt     <  1:1434451/149‑1 (MQ=255)
                                                                                                                                            atCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCttt     <  1:592378/149‑1 (MQ=255)
                                                                                                                                            atCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCttt     <  1:1884100/149‑1 (MQ=255)
                                                                                                                                            atCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCttt     <  1:965682/149‑1 (MQ=255)
                                                                                                                                            atCGATCAGCCAATTCCACAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCttt     <  1:837381/149‑1 (MQ=255)
                                                                                                                                             tCGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCtttt    >  2:1834652/1‑149 (MQ=255)
                                                                                                                                              cGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCTTTTa   <  2:852289/149‑1 (MQ=255)
                                                                                                                                              cGATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCTTTTa   <  1:1030929/149‑1 (MQ=255)
                                                                                                                                               gATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCTTTTaa  <  2:1232617/149‑1 (MQ=255)
                                                                                                                                               gATCAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCTTTTaa  >  2:565330/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TACTAAATACTTTCCCCATCAAATGATTTGGCGTTTTTATTTGAATGGCAACTGATCTTGTTAGTCCCTCTATAGATTGTCCAAGTCCCAATAATGTTGCACCTATATATAATATCGCCACACTTGGAAACACATTAATAATCGATAAGCCAATTCCCCAAACTAAAACACCAATACTAAATTTAAAGATTAATCGCTTTTCTGACAGCAAACCCATAATCAATGACATTAATAAAGATGCTATACCTAAACATGATGTAGCTAGTCCATATACACCAACGCCCTCTTTTAA  >  CP000730/2621635‑2621926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: