Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,623,244 G→A intergenic (‑574/+3462) USA300HOU_2485 ← / ← sarT MFS family major facilitator transporter, cation symporter/staphylococcal accessory regulator T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,623,2440GA96.8% 547.0 / NA 189intergenic (‑574/+3462)USA300HOU_2485/sarTMFS family major facilitator transporter, cation symporter/staphylococcal accessory regulator T
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base A (99/80);  total (104/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

AATTATACTACTAAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTGGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACAATCT  >  CP000730/2623099‑2623388
                                                                                                                                                 |                                                                                                                                                
caTTATACTACTCAACATCAATCCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGc                                                                                                                                               <  1:1810536/148‑1 (MQ=255)
  ttATACTACTAAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCa                                                                                                                                             <  1:417643/149‑1 (MQ=255)
     tactacTAAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCAttt                                                                                                                                          >  1:197249/1‑149 (MQ=255)
     tactacTAAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCAttt                                                                                                                                          >  1:506834/1‑149 (MQ=255)
     tactacTAAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCAttt                                                                                                                                          >  1:1619432/1‑149 (MQ=255)
      actactAAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATTAACTAGGCCATTTg                                                                                                                                         >  1:382706/1‑149 (MQ=255)
      actactAAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTCGGCCATTTg                                                                                                                                         >  1:647686/1‑149 (MQ=255)
        tactaAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGt                                                                                                                                       <  1:1204159/149‑1 (MQ=255)
                      ccTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCttt                                                                                                                         >  1:359222/1‑149 (MQ=255)
                       cTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTc                                                                                                                        <  1:1663356/149‑1 (MQ=255)
                               gTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATcc                                                                                                                <  2:1994379/149‑1 (MQ=255)
                                  cTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTgg                                                                                                             >  1:1049182/1‑149 (MQ=255)
                                  cTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTgg                                                                                                             >  1:1554237/1‑149 (MQ=255)
                                     tCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCGTTGATTTAACTCGGCCCTTCGGTTTTGCTCTGTCTTTCGATAATCCTGGAtt                                                                                                          >  1:265197/1‑149 (MQ=255)
                                      cTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTATGTCTTTCGATAATCCTGGATTg                                                                                                         <  1:1486667/149‑1 (MQ=255)
                                       tAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGGTAAAGATTCTTTAGCAATTTTAGATTTTTTAACTGTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGt                                                                                                        >  1:1178083/1‑149 (MQ=255)
                                       tAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGt                                                                                                        >  2:192923/1‑149 (MQ=255)
                                       tAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGt                                                                                                        <  1:608070/149‑1 (MQ=255)
                                        aagccTGTTTTTGGTAAGTCTTCTCGTTTTTTCTGTTGTTTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTATTATCCATTGAATGAACTACGCCATTTGGTTTTTCTCTGTCTTTCGATAATCCTGGATTGtt                                                                                                       <  1:1297624/146‑1 (MQ=255)
                                        aaaCCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGtt                                                                                                       <  1:14033/149‑1 (MQ=255)
                                        aaaCCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTACATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGtt                                                                                                       <  2:1489849/149‑1 (MQ=255)
                                        aaaCCTGTTTTTGGTAATTCTGCTCGTGTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGtt                                                                                                       <  1:1001377/149‑1 (MQ=255)
                                         aaCCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTa                                                                                                      >  1:394390/1‑149 (MQ=255)
                                         aaCCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTa                                                                                                      <  2:1454177/149‑1 (MQ=255)
                                          aCCTGTTTTTGGTAATTCTTCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAg                                                                                                     <  1:1519902/149‑1 (MQ=255)
                                          aCCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAg                                                                                                     <  1:550056/149‑1 (MQ=255)
                                            cTGTTTTTGGTAATTCTGCTCGTTTTTTTTTTTTTTTTGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGACTGACCTAAGCCCTTTGTTTTTGCTCTGTTTTCCGTTATTctttgttttttttgt                                                                                                   >  2:1645376/1‑135 (MQ=255)
                                             tGTTTTTGGTAATTCTGCTCGTTTTTTCTTTTGATTAGCTACTGATTCTTTAGCTATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTTTCTTTCGTTAATCTTGGTTTTTTAggtt                                                                                                  >  2:1273127/1‑147 (MQ=255)
                                                ttttGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCCTTGAATGAACTGGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTa                                                                                               <  2:916227/149‑1 (MQ=255)
                                                  ttGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACt                                                                                             <  1:631475/149‑1 (MQ=255)
                                                    ggTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACGGATTCGTGTGCAAGTGTGGAGTTTCTAACTTGATCATTTGTAGCCATTGAATGAACTAGGCCTTTTGGTTTTGCTCTGTCTGTCGATAATCCTGTATTGTTAGGATTTACTgg                                                                                           <  2:791928/149‑1 (MQ=255)
                                                      tAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGc                                                                                         <  1:1869209/149‑1 (MQ=255)
                                                         ttCTGCTCGTTTTTTTTCTTTATTTGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGACCTAGGCCATTTGGTTTTGCTCTCTCTTTCTTTATTCCTGGCTTGTTAGGATTTACTGGGCCAc                                                                                      >  2:524627/1‑149 (MQ=255)
                                                          tCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACt                                                                                     >  2:1744165/1‑149 (MQ=255)
                                                             gCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTgg                                                                                  <  2:1314224/149‑1 (MQ=255)
                                                              cTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGa                                                                                 <  1:427124/149‑1 (MQ=255)
                                                               tCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGAt                                                                                >  2:633547/1‑149 (MQ=255)
                                                                   tttttCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGt                                                                            >  1:1825211/1‑149 (MQ=255)
                                                                      ttCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTgg                                                                         <  1:192923/149‑1 (MQ=255)
                                                                       tttctGGATGAGCTACCGATTCTTTAAAAAGGCAGGATGTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGt                                                                        <  1:452891/147‑1 (MQ=255)
                                                                       tctcTTGATTAGCTACTGATTCTTTAGCAATTGTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTGTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGt                                                                        <  1:1339449/149‑1 (MQ=255)
                                                                        ctctTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTc                                                                       <  1:1230599/149‑1 (MQ=255)
                                                                         tctTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCt                                                                      >  2:1675162/1‑149 (MQ=255)
                                                                          ctcgATTAACTACTGATTCTTGAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTTATTGAACTAAGCCAGTTGGTTATGCTCTGTCTTTCGATAGTTCTGTAGTGTTAGGTTTTACTGGGCCACTAGGTGGAGGTGTTCTg                                                                     >  2:344053/4‑149 (MQ=255)
                                                                          ctTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTGTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTATTGTGCCACTTGGATGAGTTtgtgtg                                                                     >  1:347364/1‑149 (MQ=255)
                                                                            tGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTCGGCCACTTGGATGAGTTGGTCTGCt                                                                   <  2:1859353/149‑1 (MQ=255)
                                                                              attgggtattGATTCTTTAGCAATTTGAGATTTTTTTACTTTCTGGTTTTGGTCCATGGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTGTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGAGGAGTTGGTCTGCTCg                                                                 <  2:702884/140‑1 (MQ=255)
                                                                              aTTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCg                                                                 <  2:1584084/149‑1 (MQ=255)
                                                                                tAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGc                                                               <  2:1962494/149‑1 (MQ=255)
                                                                                 aGCTACCGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCt                                                              <  2:1178083/149‑1 (MQ=255)
                                                                                  gCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCtt                                                             >  1:1420552/1‑149 (MQ=255)
                                                                                   cTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTtc                                                            >  1:719369/1‑149 (MQ=255)
                                                                                    tACTGATTCTTTAGCAATTTTCGATTTTTTAAATTTAACATTTTTATCCATTCAATGAACTGGGCTATTTCGGTGTGCTCTTTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTtct                                                           <  2:669073/149‑1 (MQ=255)
                                                                                         actCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTggg                                                      >  1:85585/3‑149 (MQ=255)
                                                                                         aTTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGTGTTTCTggg                                                      >  2:490629/1‑149 (MQ=255)
                                                                                          ttCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGt                                                     >  1:1710495/1‑149 (MQ=255)
                                                                                            ttttAGCAATTTTAGTTTTTTTAACTTTATCATTTTTATCCAGTTAATGTACTAGTCCATTTTGTTTGGCTCTTTCTTTCGATAATCCTGGATTGTTAGGATGTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGttt                                                   <  2:216890/148‑1 (MQ=255)
                                                                                            cTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGttt                                                   <  2:1611058/149‑1 (MQ=255)
                                                                                                 gCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAgg                                              <  2:425877/149‑1 (MQ=255)
                                                                                                  cAATTTTAGATTTTTTAACTTTATCATTTTTCTCAACTAAATGAACTGGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAggg                                             >  2:830795/1‑148 (MQ=255)
                                                                                                  cAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGt                                             >  1:228191/1‑149 (MQ=255)
                                                                                                  cAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCGCTGTCGTTCGATAATCCTGGATTGTTAGGATTGTTTGTTCTGCTTGGATGAGTTGGTCTGCCGGGCTTCTCTAGGTTTTCAggg                                             >  1:57296/1‑148 (MQ=255)
                                                                                                      tttAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCtt                                         <  2:359222/149‑1 (MQ=255)
                                                                                                           aTTTTTTAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGa                                    <  1:701848/149‑1 (MQ=255)
                                                                                                              ttttAACTTTATCATTTTTATCAATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGTCCCCTTGGATGAGTTGGTCTGCTCGGCGTCTCTGGGTTTTCAGGTCCTTTTGTATCt                                 >  2:1778295/1‑149 (MQ=255)
                                                                                                                ttAACTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCATGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGGTCttt                               >  2:948853/1‑149 (MQ=255)
                                                                                                                 tAACTTTATCATTTTTATCCGTTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCtttt                              <  2:371734/149‑1 (MQ=255)
                                                                                                                  aaCTTTATCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTg                             <  1:1067103/149‑1 (MQ=255)
                                                                                                                   aCTTTATCATTTTTATCCATTGAATTAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTgg                            <  1:683768/149‑1 (MQ=255)
                                                                                                                         tCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTtctc                      <  1:101522/149‑1 (MQ=255)
                                                                                                                         tCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTtctc                      >  1:1107965/1‑149 (MQ=255)
                                                                                                                         tCATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATCGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTtctc                      >  1:1779648/1‑149 (MQ=255)
                                                                                                                         acagttttATCCATTGAATGAACTAGGCCACTTGGTTTTGCGCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACGGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTtctc                      <  1:844403/145‑1 (MQ=255)
                                                                                                                          cATTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTtctct                     <  1:1891835/149‑1 (MQ=255)
                                                                                                                           aTTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTtctctc                    >  2:699187/1‑149 (MQ=255)
                                                                                                                           aTTTTTATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTtctctc                    >  1:600718/1‑149 (MQ=255)
                                                                                                                               ttATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCAcc                >  1:661012/1‑149 (MQ=255)
                                                                                                                               ttATCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCAcc                >  2:1234164/1‑149 (MQ=255)
                                                                                                                                 ttCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGa              <  2:1349958/148‑1 (MQ=255)
                                                                                                                                  tCCATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTAATGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTCGGTTTCTCTCCACTGac             >  2:1339157/1‑148 (MQ=255)
                                                                                                                                    catgttatgaaCTTGGCCCTTTGGTTTTGCTCTGTCTTTCGATAATCGTGGATTTTAAGGGTTTACTGGGCCACTTGGATGAGTTGGTCTTCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAAct           <  2:1161657/143‑1 (MQ=255)
                                                                                                                                    cATTGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTCCTCTGGGTTTTCAGGTCCTTTTTGACCTTTTGGTTTCTCTCCACCGAAct           <  1:1113713/149‑1 (MQ=255)
                                                                                                                                    cATTGAATGAACTAGGCCATCTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAAct           <  2:312452/149‑1 (MQ=255)
                                                                                                                                      ttGAATGAACTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGCTTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAActct         >  1:1312220/1‑149 (MQ=255)
                                                                                                                                      ttGAATGAACTAGAACATTTGGTTTTGATGTGTCTATGGGTAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAActct         >  1:1471981/1‑149 (MQ=255)
                                                                                                                                       tgaatgaaCTCAGCCATTTGGGTTTGCTCTGTCTTTCGATAATCCTGAATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGCTTTCTCTCCTCCTAActttt        <  2:239273/149‑4 (MQ=255)
                                                                                                                                       tgaatgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTa        <  1:1939559/149‑1 (MQ=255)
                                                                                                                                       tgaatgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTa        <  1:1961277/149‑1 (MQ=255)
                                                                                                                                       tgaatgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTa        <  1:924240/149‑1 (MQ=255)
                                                                                                                                       tgaatgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTa        <  1:1740054/149‑1 (MQ=255)
                                                                                                                                       tgaatgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTa        <  2:897203/149‑1 (MQ=255)
                                                                                                                                       tgaaggaaCTGGGCCATGTGGTTTTGCTCTGTCGTGCGAGAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTa        <  1:758593/149‑1 (MQ=255)
                                                                                                                                         aatgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACa      >  2:1001768/1‑149 (MQ=255)
                                                                                                                                         aatgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACa      >  2:1017794/1‑149 (MQ=255)
                                                                                                                                         aatgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTAATGGATCTGTTGGTTTCTCTCCACCGAACCCTCCa      >  2:104590/1‑149 (MQ=255)
                                                                                                                                         aatgaaCTAGGCCATTTGGTTTTGCTCTGGCTTTCGATAATCCTGGATCGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCCTCTCTGGGTTATCAGGTCCTGTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACa      >  2:1689767/1‑149 (MQ=255)
                                                                                                                                          atgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACaa     >  1:325650/1‑149 (MQ=255)
                                                                                                                                           tgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACAAt    <  2:647686/149‑1 (MQ=255)
                                                                                                                                           tgaaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGACACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACAAt    <  1:1921528/149‑1 (MQ=255)
                                                                                                                                             aaCTAGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACCATCt  >  1:831375/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AATTATACTACTAAAGATCAAACCTTTTTGCGTGCTTTCTAAACCTGTTTTTGGTAATTCTGCTCGTTTTTTCTCTTGATTAGCTACTGATTCTTTAGCAATTTTAGATTTTTTAACTTTATCATTTTTATCCATTGAATGAACTGGGCCATTTGGTTTTGCTCTGTCTTTCGATAATCCTGGATTGTTAGGATTTACTGGGCCACTTGGATGAGTTGGTCTGCTCGGCTTCTCTGGGTTTTCAGGTCCTTTTGGATCTTTTGGTTTCTCTCCACCGAACTCTACAATCT  >  CP000730/2623099‑2623388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: