Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 923,858 T→C 6.6% W53R (TGG→CGG)  lipA → lipooyl synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730923,8580TC 6.6% 177.6 / 2.2 68W53R (TGG→CGG) lipAlipooyl synthase
Reads supporting (aligned to +/- strand):  ref base T (34/29);  new base C (3/2);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.23e-01

GGAAATATTACGTAAACCGGATTGGTTGAAAATAAAATTAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGAACGTGTAGCTGAATCAGTTGAATT  >  CP000730/923719‑923997
                                                                                                                                           |                                                                                                                                             
ggAAATATTACGTAAACCGGATTGGTTGAAAATAAAATTAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTg                                                                                                                                              >  1:546355/1‑141 (MQ=255)
ggAAATATTACGTAAACCGGATTGGTTGAAAATAAAATTAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTg                                                                                                                                              >  1:663648/1‑141 (MQ=255)
        tACGTAAACCGGATTGGTTGAAAATAAAATTAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCAc                                                                                                                                     >  1:613119/1‑141 (MQ=255)
         aCGTAAACCGGATTGGTTGAAAATAAAATTAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCAcg                                                                                                                                    >  2:48586/1‑141 (MQ=255)
         aCGTAAACCGGATTGGTTGAAAATAAAATTAAACACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCAcg                                                                                                                                    <  1:222858/141‑1 (MQ=255)
          cGTAAACCGGATTGGTTGACAATAAAATTCAATACCAACGAAAACTATACAGGCCTTAAGACGATGATGAGGGCAAAAAATCTTAATACTATAAGCGAAGAAGATAAATGTACTAATATACATAAATGTTGGGGTGCAcgt                                                                                                                                   >  2:596538/1‑141 (MQ=255)
            tAAACCGAGTTGGTTGAAAATAAAATTAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCAcgtcg                                                                                                                                 <  2:525501/141‑1 (MQ=255)
                 cgtaTTGGTTTAAAATAAAACTAAAAACCCACGTGAACTAGAATCCACATAAGAGGCTGAGCAGGCAAAAAAATCGTAATACCGTATTTGAAGAAGCTAGATATCATCATATACATGATTGTTGGGGTGCACGTCGTACAg                                                                                                                            <  2:276561/138‑1 (MQ=255)
                   gATTGGTTGAAAATAAAATGAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCg                                                                                                                          <  2:121102/141‑1 (MQ=255)
                    aTTGGTTGAAAATAAAATTAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGa                                                                                                                         <  1:344788/141‑1 (MQ=255)
                                 aaaatTAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGAtttt                                                                                                            <  2:454191/141‑1 (MQ=255)
                                 aaaatTAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATGTATGAtttt                                                                                                            >  1:656342/1‑141 (MQ=255)
                                    atTAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTCGGGGTGCACGTCGTACAGCGACATTTATGATTTTAg                                                                                                          <  1:629169/140‑1 (MQ=255)
                                    atTAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTCGGGGTGCACGTCGTACAGCGACATTTATGATTTTAg                                                                                                          >  2:629169/1‑140 (MQ=255)
                                    atTAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTCGGGGTGCACGTCGTACAGCGACATTTATGATTTTAg                                                                                                          <  1:629167/140‑1 (MQ=255)
                                    atTAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTCGGGGTGCACGTCGTACAGCGACATTTATGATTTTAg                                                                                                          >  2:629167/1‑140 (MQ=255)
                                      tAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGAGTGCACGTCGAACAGCGACCTTTATGATTTTAGGTg                                                                                                       >  2:231906/1‑141 (MQ=255)
                                        aaTACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGATAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGcc                                                                                                     >  2:422782/1‑141 (MQ=255)
                                         aTACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATCCTTTAGGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCg                                                                                                    <  1:563119/141‑1 (MQ=255)
                                         aTACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAAACTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGAGCCg                                                                                                    >  2:301317/1‑141 (MQ=255)
                                             cAACGAAAACTAGACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATg                                                                                                <  1:313117/141‑1 (MQ=255)
                                                     aCTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAagag                                                                                        >  2:353841/1‑141 (MQ=255)
                                                      cTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAGGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTAAGGTCCCGTATGTACAGGAGc                                                                                       >  2:188103/1‑141 (MQ=255)
                                                      cTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGc                                                                                       >  1:473486/1‑141 (MQ=255)
                                                      cTATACAGGACTTAAGAAGATGATGAGCAAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGTGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGc                                                                                       <  1:381174/141‑1 (MQ=255)
                                                         taCAGGACTTAAGAAGATGATGAGGTAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTg                                                                                    <  1:376077/141‑1 (MQ=255)
                                                         taCAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTTTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGACTTTAGGTGCCGTATGTACAAAAGCttt                                                                                    >  2:25823/1‑140 (MQ=255)
                                                         taCAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTg                                                                                    <  2:734268/141‑1 (MQ=255)
                                                             ggACTTAAAAAAATGAGTAGGGAAAAAAATCTTAATACTGTATGGGAAGAAGCTAAATGTCCTAATATACAGGAATGTTGGGTTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGt                                                                                <  2:370375/141‑1 (MQ=255)
                                                               acgtAAGTAGATGATGAGGGCAGGAAATCTGAATACTGTATGTGAAGAAGCGAAATGTCCTCAGATACAGGAATGTTGGGGTGCACATCGTACAGCGACAGTTATGATTTTATGTGCCGTATGTACAAGAGCTTGTCGttt                                                                              <  1:501727/138‑1 (MQ=255)
                                                                  tAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTtg                                                                           <  2:764769/141‑1 (MQ=255)
                                                                  tAAGAAAATGAAGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTtg                                                                           <  1:730708/141‑1 (MQ=255)
                                                                   aagaagATGATGAGGGAAAAAAATCTTAATACGGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTtgt                                                                          >  2:493987/1‑141 (MQ=255)
                                                                         atgatgAGTGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGtt                                                                    <  1:592941/141‑1 (MQ=255)
                                                                          tgatgaGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGAGGTGCACGTCGTACAGCGACATGTATGATTTTAGGGGCCGGATGTACAAGAGCTTGTCGTTTTTGTGCGGTTa                                                                   >  2:733778/1‑141 (MQ=255)
                                                                          tgatgaCGGAAAAAAATCCTAATACTGTATGTGAAGAAGCTAAATGTCCTAATAGACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTa                                                                   <  1:259144/141‑1 (MQ=255)
                                                                          agatgaGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTACGATTTTAAGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTa                                                                   <  2:149817/140‑1 (MQ=255)
                                                                          aaagtaGGGAAAAAAATCTTAATCCTGTATGTGAAGAAGCTAAAAGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTa                                                                   <  1:315903/136‑1 (MQ=255)
                                                                            atgaGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAg                                                                 >  1:128119/1‑141 (MQ=255)
                                                                             tgaGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGa                                                                >  1:227252/1‑141 (MQ=255)
                                                                             tgaGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGAGGTGCCCGTCGTACAGCGACCTTTATGATTGTAGTTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAgg                                                                >  2:156167/1‑140 (MQ=255)
                                                                              gaGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGAc                                                               <  2:689399/141‑1 (MQ=255)
                                                                                gggAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGTTTAAGGCAg                                                             <  2:452209/141‑1 (MQ=255)
                                                                                  gAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGt                                                           <  1:392465/141‑1 (MQ=255)
                                                                                                     tATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGGCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGAtt                                        <  2:128750/141‑1 (MQ=255)
                                                                                                     tATGTGAAGAAGCTAAATGTCACATAGCACATGAATGATCGCGTGTGCTTTGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGAtt                                        <  1:560333/141‑1 (MQ=255)
                                                                                                         tgAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGCGCATGGCGGTTGTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTaaa                                    >  1:59323/1‑141 (MQ=255)
                                                                                                          gaagaaGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGGGCTTGTCGTTGTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTCAAAt                                   >  2:437106/1‑141 (MQ=255)
                                                                                                            agaagCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGATTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGa                                 >  2:75691/1‑141 (MQ=255)
                                                                                                               aggtAAATCTCCTAATAGACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGGTTTAAATGAGcc                             <  2:164749/138‑1 (MQ=255)
                                                                                                               agCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAAGGAACTTGATTTAAATgtgtc                              >  2:409278/1‑137 (MQ=255)
                                                                                                                   aaaTGTCCTAATATACATGGGTACCAAGATGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGaa                         >  1:375323/1‑141 (MQ=255)
                                                                                                                    aaTGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGAAc                        >  2:479225/1‑141 (MQ=255)
                                                                                                                     aTGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGAACg                       <  2:140563/141‑1 (MQ=255)
                                                                                                                      tGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAACTGAGCCTGAAccg                      >  1:705221/1‑139 (MQ=255)
                                                                                                                         ccTACTATACATAAATGTTGAGGTCCACGTCGTACAGCGACACTTCTGATCTTAGGTGATTGATCGACAAGAGCTTGTCGTTTTTGTGGGGTTAAGAACAGTTTACCTAATGAACTTGATTTAAATTAGCCTGAACGTGTa                   >  2:319182/1‑141 (MQ=255)
                                                                                                                           tAATCTACATGAATGTTGGGGTGAAGAATCGGCATGGGTATTGATCATTTTTGGTCCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGAACGTGTAGc                 <  1:293798/141‑1 (MQ=255)
                                                                                                                           caaTATAACTGAATGTTGGGTTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGAACGTGTAGc                 <  1:596538/140‑1 (MQ=255)
                                                                                                                            aaTATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGAACGTGTAGCt                >  2:297920/1‑141 (MQ=255)
                                                                                                                              tataCATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGAACGTGTAGCTGa              >  1:381568/1‑141 (MQ=255)
                                                                                                                                 aCATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGAACGTGTAGCTGAATc           >  2:121495/1‑141 (MQ=255)
                                                                                                                                 aCATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGAACGTGTAGCTGAATc           >  2:268420/1‑141 (MQ=255)
                                                                                                                                    tGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGCGTTAAATGAGCCTGGACGTGTAGCTGAATCAgg        >  1:667564/1‑140 (MQ=255)
                                                                                                                                       aTGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGAACGTGTAGCTGAATCAGTTGa     <  1:48586/141‑1 (MQ=255)
                                                                                                                                        tGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGAACGTGTAGCTGAATCAGTTGat    >  2:456280/1‑140 (MQ=255)
                                                                                                                                        tGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGAACGTGTAGCTGAATCAGTTGaa    >  2:111137/1‑141 (MQ=255)
                                                                                                                                        tGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGAACGTGTAGCTGAATCAGTTGaa    >  2:456360/1‑141 (MQ=255)
                                                                                                                                         gTTGGGGTGCACGTCGTACAGCGACATTTATGATGTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGAACGTGTAGCTGAATCAGTTGAAt   >  1:184387/1‑141 (MQ=255)
                                                                                                                                          gtGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGCACAAGAGCTTGTCGTTTTTGTGCGGCTAAGACAGGTTTACCTAATGAACTTCATTTAACTGATTGCTGGCGTTTAGCTGAATCAGCTGAAtt  <  1:570663/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                             
GGAAATATTACGTAAACCGGATTGGTTGAAAATAAAATTAAATACCAACGAAAACTATACAGGACTTAAGAAGATGATGAGGGAAAAAAATCTTAATACTGTATGTGAAGAAGCTAAATGTCCTAATATACATGAATGTTGGGGTGCACGTCGTACAGCGACATTTATGATTTTAGGTGCCGTATGTACAAGAGCTTGTCGTTTTTGTGCGGTTAAGACAGGTTTACCTAATGAACTTGATTTAAATGAGCCTGAACGTGTAGCTGAATCAGTTGAATT  >  CP000730/923719‑923997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: