Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,499,424 A→G 5.4% intergenic (‑1/‑182) scrA ← / → USA300HOU_2363 PTS family sucrose porter component IIBC/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,499,4240AG 5.4% 216.6 / 3.2 74intergenic (‑1/‑182)scrA/USA300HOU_2363PTS family sucrose porter component IIBC/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (40/30);  new base G (2/2);  total (42/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

TGTTTAGCGCCTCTTCATTTACTAAACTTTCATCATTTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTCATTTTTATTGCTATAC  >  CP000730/2499285‑2499564
                                                                                                                                           |                                                                                                                                            
tGTTTAGGGCCTCATCAGTTACTAAACTTTCATCATTTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCctat                                                                                                                                             >  2:358671/1‑141 (MQ=255)
  tttAGCGCCTCTTCATTTACTAAACTTTCATCATTTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCatatat                                                                                                                                           >  2:231043/1‑141 (MQ=255)
   ttAGCGCCTCTTCATTTACTAAACTTTCATCATTTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATAtt                                                                                                                                          <  1:150806/141‑1 (MQ=255)
       cgcCTCTTCATTTACTAAACTTTCATCATTTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGc                                                                                                                                      >  1:415901/1‑141 (MQ=255)
        gcCTCTTCATTTACTAAACTTTCATCATTTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCAGGCTATCGCGTTCAAACTTTCTTCTGCGGATTGCTTATAATTCATATATTTAGcc                                                                                                                                     >  1:601208/1‑141 (MQ=255)
        ccctgcgcATTCACGAAACTTGCATCATTTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTCCGGATTGCTTAGAATTCATATATTTAGcc                                                                                                                                     <  1:536079/134‑1 (MQ=255)
            ctTCATTTACTAAACTTTCATCATTTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGcctcct                                                                                                                                 >  2:679384/1‑141 (MQ=255)
                   tACTAAACTTTCATCATTTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGa                                                                                                                          >  2:610901/1‑141 (MQ=255)
                   tACTAAACTTTCATCATTTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGa                                                                                                                          <  1:657337/141‑1 (MQ=255)
                    aCTAAACTCTCATCCTTTAAAACTAATCGTGGCCTCGTGGTACAATGCGCCATTGCATCTCTATTCTCTTCTCCGCCTACCGCGTTCAACATTTCTTCTGCGGATTGCTTATACTTCATATATTTAGCCTCCTTATTAGaa                                                                                                                         <  2:261712/141‑1 (MQ=255)
                     cTAAACTTTCATCATGTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAAc                                                                                                                        >  1:578089/1‑141 (MQ=255)
                        aaCTTTCATCATTTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCgg                                                                                                                     >  2:317084/1‑141 (MQ=255)
                         aCTTTCATCATTTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTTTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGt                                                                                                                    <  2:161945/141‑1 (MQ=255)
                           tttCATCATTTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTc                                                                                                                  <  1:52785/141‑1 (MQ=255)
                              catcatTTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCAt                                                                                                               >  1:651577/1‑141 (MQ=255)
                                           aattCGTAAGCTCTTTGCACAATGCCCCATTGCATCTAAATTCTCTTCCCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTa                                                                                                  <  1:222885/138‑1 (MQ=255)
                                            aaTCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATGTATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTAt                                                                                                 >  1:184594/1‑141 (MQ=255)
                                            aaTCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATGTATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTAt                                                                                                 >  1:257750/1‑141 (MQ=255)
                                            aaTCGTAGTCTCGTTGCACAATGCGCCATGACTTCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTAt                                                                                                 >  1:770107/1‑141 (MQ=255)
                                              tCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTAttaccatttcttg                                                                                               >  1:653215/1‑130 (MQ=255)
                                                gtcgactcGTTGCACTTTGAGCCATGGCATGTAAGTTCTATTATATGACCATCGCGTTGAAAGTATCTTCGGGGGATTGCTTATAATCGATATATTTAGCCTCCTAATTAGAACCGGTCCCCTTTGTTCCATTTTCTTTTa                                                                                             >  2:40683/6‑141 (MQ=255)
                                                gTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTa                                                                                             >  1:279414/1‑141 (MQ=255)
                                                gTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTa                                                                                             >  1:658701/1‑141 (MQ=255)
                                                  aGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATGTATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAAc                                                                                           <  2:257750/141‑1 (MQ=255)
                                                  aGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATGTATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAAc                                                                                           <  2:184594/141‑1 (MQ=255)
                                                  aGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAAc                                                                                           <  1:197744/141‑1 (MQ=255)
                                                     ctcGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCgg                                                                                        >  1:362682/1‑141 (MQ=255)
                                                      tcGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGt                                                                                       >  1:454090/1‑141 (MQ=255)
                                                      tcGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGt                                                                                       >  2:771524/1‑141 (MQ=255)
                                                       cGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGtt                                                                                      >  2:527188/1‑141 (MQ=255)
                                                         ttGCACAATGCGCCATTGCATCTAACTTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTTTTCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTcc                                                                                    <  1:564913/141‑1 (MQ=255)
                                                              caATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCaaaaa                                                                               <  1:344795/141‑1 (MQ=255)
                                                               attGCGCCATTGCATCTAAATTATCTTCTCCGCCTATCGCGTTCAAAATTTCCTCTGCGGCTTGCTTCTAATTCATATATTACGCCTCCTATTTAGAACCGGTTCCATTTATTCCACTTCATTATAACCGGTTCCaaaact                                                                              >  2:195995/3‑139 (MQ=255)
                                                               aaTGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAat                                                                              >  1:274796/1‑141 (MQ=255)
                                                               aaTGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATAAGATTTCCGAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAat                                                                              >  1:563607/1‑141 (MQ=255)
                                                                   cgcCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTCGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATAttt                                                                          <  1:514661/141‑1 (MQ=255)
                                                                     ccgttGCATCTAAATACTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATCATTCATATATTTAGCCTCCTAGTTAGCACCGGTTCCATTTATTCCACTGTATTGTCACCGGTTCCAAAAATCTATGc                                                                        >  2:441387/4‑141 (MQ=255)
                                                                     ccATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCCCTTTATTGTAACCGGTTCCAAAAATATTTGc                                                                        >  1:29884/1‑141 (MQ=255)
                                                                     ccATGGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGc                                                                        >  2:713283/1‑141 (MQ=255)
                                                                        ttGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAac                                                                     <  1:649190/141‑1 (MQ=255)
                                                                        ttGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAac                                                                     <  1:649184/141‑1 (MQ=255)
                                                                          gCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGcaccac                                                                   >  1:188785/1‑141 (MQ=255)
                                                                          gCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAacac                                                                   >  2:107888/1‑141 (MQ=255)
                                                                                aaaTTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCtatt                                                             <  2:693377/141‑1 (MQ=255)
                                                                                 aaTTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCtattt                                                            >  1:299799/1‑140 (MQ=255)
                                                                                 aaTTCTCTTCTCCGCCTATCGCGGTCAAAATTTTTTTTGCGGATTGCTTATAACTCAAATATTTAGCCTCCTAATTAGAACCGGATCCACTTATCCGCCTGTATTGTAACCGGTACCAATTATTGTTGCAACActtgattg                                                            <  2:166467/141‑7 (MQ=255)
                                                                                        ttctcCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCAtttt                                                     >  2:548563/1‑141 (MQ=255)
                                                                                        ttctcCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCAtttt                                                     >  1:263358/1‑141 (MQ=255)
                                                                                           tcCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACGCTCTATTATCATTTtata                                                  >  1:242400/1‑141 (MQ=255)
                                                                                           tcCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTTTCTTATCATTTtatt                                                  >  1:650712/1‑140 (MQ=255)
                                                                                            ccGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTtatat                                                 >  2:556024/1‑141 (MQ=255)
                                                                                              tccTATCTCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTTTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATaa                                               <  2:628594/140‑1 (MQ=255)
                                                                                              gCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATaa                                               <  1:711841/141‑1 (MQ=255)
                                                                                                    cgcgTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACAACCTATTATCATTTTATATAAACACCt                                         >  2:309825/1‑141 (MQ=255)
                                                                                                     gcgTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACCCTCTATTATCATTTTATATAAACACCTa                                        >  1:12671/1‑141 (MQ=255)
                                                                                                      cgTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTac                                       >  2:81423/1‑141 (MQ=255)
                                                                                                      cgTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTac                                       <  2:601144/141‑1 (MQ=255)
                                                                                                        ttCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTacac                                     <  1:548563/141‑1 (MQ=255)
                                                                                                                 tctcctGCGGATTGCATATAATTCATATATTTAGCCCACTAACTAGAACTGTTTCCATTTACTCCACTTTATTGAAAAAAAAAAAAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGa                            <  2:738331/141‑1 (MQ=255)
                                                                                                                    tctGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAg                         <  1:558472/141‑1 (MQ=255)
                                                                                                                      tGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAgcg                       <  2:57963/141‑1 (MQ=255)
                                                                                                                      tGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAgcg                       <  2:707122/141‑1 (MQ=255)
                                                                                                                         cgATTGCTTATAACTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGcat                    <  2:45341/140‑1 (MQ=255)
                                                                                                                           aTTGCTTATAATTCATATCTTTAGCAGGCAAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTCTATAAACACCTACACCATTTTTGATAGCGcattc                  >  1:374763/1‑141 (MQ=255)
                                                                                                                           aTTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGcattc                  <  2:770107/141‑1 (MQ=255)
                                                                                                                            tttgttATAAGTCGTATATGTAGCCCCCTGATTAGAACCTGTTCCATTTATTCCACTTTATTGTACCCGCTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTCCACCATTTTTGATAGCGcattca                 <  2:338814/137‑1 (MQ=255)
                                                                                                                            ttGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGcattca                 >  1:324730/1‑141 (MQ=255)
                                                                                                                            ttGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGcattca                 <  1:39424/141‑1 (MQ=255)
                                                                                                                            ttGCTTATAATTAATATATTTGGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACAATCTATTATCATGTTATATAAACACCTACACCATTTTTGATAGCGccttca                 >  1:401630/1‑141 (MQ=255)
                                                                                                                              gCTTATAATTCATATATTTATCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGcattcatt               <  1:663770/141‑1 (MQ=255)
                                                                                                                              gCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGcattcatt               >  1:451982/1‑141 (MQ=255)
                                                                                                                                 tataATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTCAttttt            >  2:260830/1‑141 (MQ=255)
                                                                                                                                   taATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTCATTTTTAt          <  2:29884/141‑1 (MQ=255)
                                                                                                                                   taATTCATATATTTAGCCTCCCAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTCATTTTTAt          <  1:637114/141‑1 (MQ=255)
                                                                                                                                        cATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTCATTTTTATTGCta     >  1:199679/1‑141 (MQ=255)
                                                                                                                                        cATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAAACTTTATGCTTTTTTTGATAGCGCATTCCTTTTTATTGCta     >  2:160846/1‑141 (MQ=255)
                                                                                                                                           atatTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTCATTTTTATTGCTAtac  >  1:100465/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TGTTTAGCGCCTCTTCATTTACTAAACTTTCATCATTTAAAACTAATCGTAGTCTCGTTGCACAATGCGCCATTGCATCTAAATTCTCTTCTCCGCCTATCGCGTTCAAAATTTCTTCTGCGGATTGCTTATAATTCATATATTTAGCCTCCTAATTAGAACCGGTTCCATTTATTCCACTTTATTGTAACCGGTTCCAAAAATATTTGCAACACTCTATTATCATTTTATATAAACACCTACACCATTTTTGATAGCGCATTCATTTTTATTGCTATAC  >  CP000730/2499285‑2499564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑