Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,027,751 C→G 100% V66L (GTA→CTA)  vraS ← sensor histidine kinase VraS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,027,7510CG100.0% 57.5 / ‑6.5 22V66L (GTA→CTA) vraSsensor histidine kinase VraS
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base G (8/12);  minor base A (1/0);  total (10/12)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACAAGTGTATGGTATAAATCTAACGTTTCACTATATATTTCTATATTTTGATCATTAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCT  >  CP000730/2027612‑2027890
                                                                                                                                           |                                                                                                                                           
aCAAGTGTATGGTATAAATTTAACGTTTCACTATATATTTCTATATTTTGATCATTAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGCTTGATTTTGTAAGCGAGTAc                                                                                                                                             <  1:175501/140‑1 (MQ=255)
aCAAGTGTATGGTATAAATCTAACGTTTCACTATATATTTCTATATTTTGATCATTAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTAc                                                                                                                                             <  1:253049/140‑1 (MQ=255)
         tGGTATAAATCTAACGTTTCACTATATATTTCTATATTTTGATCATTAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTAGCGAACcaac                                                                                                                                    <  1:160816/140‑1 (MQ=255)
                 aTCTAACTTTTCACTATTTATTTCTATATTTTGATCATTAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTCGCGACCCAACAATAATac                                                                                                                            <  2:267116/140‑1 (MQ=255)
                    tAACGTTTCACTATATATTTCTATATTTTGATCATTAATGCCAACTGTTTCGCCTTCCATTGATGGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTACGCGTGTAGCGAACAGAGAATATTacaaa                                                                                                                         >  1:11880/1‑138 (MQ=255)
                        gTTTCATTATATATTTCTTTATTTTGATCATTAATGTCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTGGCTGATTGATTTTGTAAGCGAGTAGCGAACCAACAATAATACACaataa                                                                                                                     <  1:27301/140‑1 (MQ=255)
                        gTTTCACTATATATTTCTATATTTTGATCATTAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTAGCGAACCAACAATAATACACaataa                                                                                                                     <  1:33023/140‑1 (MQ=255)
                          ttCACTATATATTTCTATATTTTGATCATTAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTAGCGAACCAACAATAATACACAataata                                                                                                                   <  1:267292/140‑1 (MQ=255)
                            cACTTTATATTTCTATATTTTGATCATTAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTAGCGAACCAACAATAATACACAATAATatg                                                                                                                 <  1:31566/140‑1 (MQ=255)
                            cACTATATATTTCTATATTTTGATCATTAATGCCAACTGTTTCTCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTAGCGAACCAACAATAATACACAATAATatg                                                                                                                 >  2:145682/1‑140 (MQ=255)
                                              ttttATCATGAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTAGCGAACCAACAATAATACACAATAATCTAATCATGCGATTtcaaaat                                                                                               <  2:243505/140‑1 (MQ=255)
                                                  aTCATTAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTAGCGAACCAACAATAATACACAATAATATGATGATGAGATTtaaaaataaaa                                                                                           >  1:191433/1‑140 (MQ=255)
                                                          tGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTAACGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTgat                                                                                   >  1:1439/1‑138 (MQ=255)
                                                                cTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTAGCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCg                                                                             >  1:157103/1‑140 (MQ=255)
                                                                 tGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTAGCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGa                                                                            >  2:266616/1‑140 (MQ=255)
                                                                                 gAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTAGCGAACCAACAATAATTTTTTTTTTTTTTATTATTAGATTAAAAAATAAAAAAACTGGTGTTCCTATTATTTGTGTCTaaac                                                            >  2:5926/1‑139 (MQ=255)
                                                                                          aaaggcggcatAATCCAATCATTTTGCTGATGTATTTTGTAAGCGAAGAGCGGACCAAAAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTg                                                   <  2:83209/130‑1 (MQ=255)
                                                                                          aTTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTAGCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTg                                                   >  2:192942/1‑140 (MQ=255)
                                                                                                 agttgatacAATCAGTATGCAGATTGATTTTGTAAGCGAGTAGCGAACCAACAATAATACACAATAATATGATGATTAGAAAAAAAAAAAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAg                                            <  2:211131/132‑1 (MQ=255)
                                                                                                        ccAATCATTTTGCTGATTGATTTTGTAAGCGAGTAGCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGatat                                     <  1:172069/140‑1 (MQ=255)
                                                                                                                 atGCCGACTGATTTTGTAAGCACGTAGCGAACCAACAAGAAGACACAATAATATGAATATTAGAATTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACaaaaa                            <  2:56598/139‑1 (MQ=255)
                                                                                                                                gTAAGCGAGTAGCGAACTAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACa             <  1:266616/140‑1 (MQ=255)
                                                                                                                                gTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACa             >  1:189635/1‑140 (MQ=255)
                                                                                                                                  aaGCGAGTAGCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATTAAACATACCTTAAAAAAAGATAATATTTACAAAAACTTTATCGATGAACAGa           >  1:3523/1‑140 (MQ=255)
                                                                                                                                           ccGAACCAACAATAATACACAATAAGATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCt  <  1:234930/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ACAAGTGTATGGTATAAATCTAACGTTTCACTATATATTTCTATATTTTGATCATTAATGCCAACTGTTTCGCCTTCCATTGAACGCTCAATTTGCGTCTTAATCCAATCATTTTGCTGATTGATTTTGTAAGCGAGTACCGAACCAACAATAATACACAATAATATGATGATGAGATTTAAAAATAAAAAAACTGGTATTCCGAATATTTGTGTATAAAACATACCTTGAAAATAGATGATATTTACAAAAACTTTATCGATGAACAGAAATGCAGCT  >  CP000730/2027612‑2027890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: