Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,265 C→T 31.3% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2650CT31.3% 391.5 / 282.9 395intergenic (+323/‑811)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (132/135);  new base T (61/61);  total (197/198)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

Only 100 of 401 total aligned reads displayed.

GCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAA  >  CP000730/76128‑76405
                                                                                                                                          |                                                                                                                                            
       atttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctact                                                                                                                                    <  1:25669/141‑1 (MQ=255)
                  ataGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGatat                                                                                                                         >  2:239920/1‑141 (MQ=255)
                       gtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAtt                                                                                                                    >  2:298236/8‑141 (MQ=0)
                          aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                                                                                                                 <  2:220410/137‑1 (MQ=2)
                           aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTTACACAAAGGAGTCGTCAATGTGATCTATTTCAGATATAAACAATTTAACAAGTATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                                                                                                                <  2:80486/138‑2 (MQ=11)
                           aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTCCTCTCTAAGATATGCATTGAgc                                                                                                                >  2:359734/4‑140 (MQ=1)
                            tgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGtt                                                                                                               >  1:317100/3‑141 (MQ=2)
                             taGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTa                                                                                                              >  2:170786/1‑141 (MQ=37)
                              cggTTCTGTTGCAAAGTTGACAAGTAAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTAt                                                                                                             >  1:185571/2‑141 (MQ=11)
                                 ttCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAAGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGt                                                                                                          <  1:167902/141‑1 (MQ=255)
                                     tttGCAAAGGGTAATTTATAATATAATTGTAAAAAAAAGTAGTCTTCGATAATTACTATGTCAGATATATACAATTTGATAGGAGTGTTATCACTGTAGCTGTTACCTACTATATAAGGTATGCATTGAGTTATCGTGaga                                                                                                      <  2:132112/140‑3 (MQ=255)
                                       tGCAAAGTTCAATCTATAGTATAAATTTAACAAAAAGGAGTCTTCTGAATGGACTCTAGCTGATATACACAAGCGAACAAGGATGTTATAACTGTTGCCGTTGGCTACTATCTAAGATATGCATTTAGTTGTCGTGatata                                                                                                    >  1:279700/1‑141 (MQ=11)
                                         cAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc                                                                                                  >  2:13696/1‑141 (MQ=35)
                                         cAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc                                                                                                  >  2:19955/1‑141 (MQ=35)
                                                tgtatttataatataaTTTTAACAAAAAGGAGACTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGCTGTTTTCACTGTAGCAGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATAACTGAAat                                                                                            >  2:30686/7‑141 (MQ=1)
                                                   atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTTAAATAtt                                                                                         >  1:33416/4‑141 (MQ=0)
                                                    tCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa                                                                                        <  1:368696/141‑1 (MQ=34)
                                                      gataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAg                                                                                      >  2:4064/2‑141 (MQ=17)
                                                        taatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                       <  1:30250/135‑1 (MQ=16)
                                                        taatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAgag                                                                                    <  2:227676/138‑3 (MQ=12)
                                                         aGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGa                                                                                   <  2:367715/141‑1 (MQ=17)
                                                          gTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGaa                                                                                  >  2:151235/1‑141 (MQ=17)
                                                            ataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACg                                                                                >  2:246971/1‑141 (MQ=17)
                                                              aaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtg                                                                              >  2:226113/1‑141 (MQ=17)
                                                              aaTTTTAACAAAAAGGAGTCCTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtg                                                                              <  2:327242/141‑1 (MQ=12)
                                                               aTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtgg                                                                             <  2:184367/141‑1 (MQ=17)
                                                                 tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggtg                                                                           <  1:60631/141‑1 (MQ=12)
                                                                 tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggtg                                                                           >  1:42272/1‑141 (MQ=17)
                                                                  ttAACAATAAGGAGACATCCTGATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                                                          <  1:238525/141‑1 (MQ=11)
                                                                  ttAACAAAAAGGAGTCTTCTGGATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                                                          <  1:128571/141‑1 (MQ=17)
                                                                     aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaaa                                                                       >  1:343413/1‑141 (MQ=12)
                                                                      cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGTCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTTAAATATTAAGGGAAAGTGGTGTaacg                                                                      >  2:276850/1‑139 (MQ=255)
                                                                        aaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGt                                                                    >  1:215030/1‑141 (MQ=17)
                                                                            ggAGTCTTCTGTATGAACTATTTCAGATCTAGACAATTTAGCAAGGATGTTATCACTGTTGCCGTTGGCGACTATCTAAGATGTGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcat                                                                >  1:151789/1‑141 (MQ=11)
                                                                             gAGTCTTCTGTATGGACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatc                                                               <  2:164934/141‑1 (MQ=17)
                                                                             gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatc                                                               <  1:80973/141‑1 (MQ=12)
                                                                              aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatca                                                              >  1:337075/1‑141 (MQ=12)
                                                                               gccttctGGATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTTCGATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTcatcat                                                             <  1:182506/139‑1 (MQ=11)
                                                                               gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAAAGTTaatcat                                                             >  2:284752/1‑141 (MQ=12)
                                                                               gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAAGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGGGTAAACGTTcatcat                                                             <  2:3346/141‑1 (MQ=12)
                                                                                tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCAtt                                                            <  1:205857/141‑1 (MQ=17)
                                                                                 cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTc                                                           >  1:23244/1‑141 (MQ=17)
                                                                                  ttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCa                                                          <  1:11427/141‑1 (MQ=12)
                                                                                   tctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCaa                                                         <  1:352135/141‑1 (MQ=17)
                                                                                    ttGTATGAACTATTTCAGATATAAACAATTTCACATGGATTGTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGCGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAAc                                                        <  1:98376/140‑1 (MQ=11)
                                                                                    ctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAAc                                                        <  1:361817/141‑1 (MQ=17)
                                                                                     tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACg                                                       <  2:193771/141‑1 (MQ=17)
                                                                                       tATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACgat                                                     <  2:337498/141‑3 (MQ=12)
                                                                                           aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGCAATATTAAGAGAACGTGGTGTAAACGTTCATCATTAAACGATCTAc                                                 >  1:294185/1‑141 (MQ=11)
                                                                                            aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGATATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                <  2:156988/141‑1 (MQ=255)
                                                                                            aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                <  1:157181/141‑1 (MQ=255)
                                                                                            aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAcc                                                <  2:177191/141‑1 (MQ=255)
                                                                                               aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                             <  1:270376/141‑1 (MQ=255)
                                                                                                ttgcAGATATAAATAAGTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAACCGTTCATCATTCAACGATCTACCGTTg                                            <  2:160126/138‑1 (MQ=11)
                                                                                                 ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTCGCTGTGGCGTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTgg                                           <  1:356828/141‑1 (MQ=11)
                                                                                                  tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                          >  2:112823/1‑141 (MQ=17)
                                                                                                  tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                          <  1:120079/141‑1 (MQ=255)
                                                                                                  tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                          <  2:343413/141‑1 (MQ=255)
                                                                                                   cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGt                                         >  2:351283/1‑141 (MQ=17)
                                                                                                    aGATTTAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGtt                                        <  2:322573/141‑1 (MQ=17)
                                                                                                    aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGtt                                        >  2:381007/1‑141 (MQ=17)
                                                                                                      atatAAACCATTTAACAAGGATGTTATCACTGTAGCCGGTGGCTACGATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCa                                      <  1:145361/141‑1 (MQ=11)
                                                                                                      atatAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCa                                      >  2:378295/1‑141 (MQ=17)
                                                                                                       tataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCaa                                     <  2:254810/141‑1 (MQ=17)
                                                                                                        ataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                    <  2:205130/141‑1 (MQ=17)
                                                                                                         taaaaatattAACAATGCTGTTACCACTTCAGCCGTTTGCTAATATCCAAGGGACGCACTGAGTTATCGTGATATATCTGAAATATTAAGTGAACGTGGTCTAAACGTTCATCATTCAACGGTCTCGCGTTGGGTTCAAGa                                   <  1:199832/133‑1 (MQ=2)
                                                                                                            aCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAAAGGTCTACCGTTGGGTTCAAGAata                                <  1:244786/141‑1 (MQ=17)
                                                                                                            aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACCTTCATCATTCAACGATCTACCGTTGGGTTCAAGAata                                <  2:95099/141‑1 (MQ=255)
                                                                                                              aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                              <  1:73317/141‑1 (MQ=255)
                                                                                                                ttgaaCAGGGATGTGATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                            <  2:209392/138‑1 (MQ=11)
                                                                                                                 ttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGccc                           >  2:44548/1‑141 (MQ=17)
                                                                                                                 ttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATATAACGTTGGGTTCAAGAATATGccc                           >  1:247927/1‑141 (MQ=11)
                                                                                                                 ttAACAAGGATGTTATCACTGCAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAGTAAGAGAACGTGCTGTAAACGTTCATCATTCAACGCTCTACCGTTGGGTTCAAGAATATGccc                           >  2:262166/1‑141 (MQ=11)
                                                                                                                   aaCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCa                         >  1:44182/1‑141 (MQ=17)
                                                                                                                   aaCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCa                         >  1:235968/1‑141 (MQ=17)
                                                                                                                    aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTATACCGTTGGGTTCAAGAATATGCCCCaa                        >  1:265813/1‑141 (MQ=17)
                                                                                                                    aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                        >  1:134214/1‑141 (MQ=255)
                                                                                                                           tGTTACCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAACAATATGCCCCAATTTtata                 <  1:202961/141‑1 (MQ=11)
                                                                                                                            gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                >  1:177425/1‑141 (MQ=255)
                                                                                                                            gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                >  1:51775/1‑141 (MQ=255)
                                                                                                                            gTTATCACTGTAGCCGTTGACTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                >  1:228692/1‑141 (MQ=11)
                                                                                                                             ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc               >  1:221002/1‑141 (MQ=255)
                                                                                                                               aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGGTCAAGAATATGCCCCACTTTTatataca             >  2:144345/1‑138 (MQ=255)
                                                                                                                               aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa             >  2:12334/1‑141 (MQ=255)
                                                                                                                               aTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAGTTTATATCaa             >  1:140482/1‑141 (MQ=255)
                                                                                                                                 cACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt           >  1:77445/1‑141 (MQ=25)
                                                                                                                                 cACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCGTTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt           >  1:106066/1‑141 (MQ=255)
                                                                                                                                 cACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAAAGATGTGACGTTGAGTTCAAGAATATGCCCCAATTTTATATCAAAt           >  1:198174/1‑141 (MQ=11)
                                                                                                                                 cACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGGTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt           >  1:70050/1‑141 (MQ=255)
                                                                                                                                  aCTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAtt          >  2:56826/1‑141 (MQ=25)
                                                                                                                                   cTGTAGCCGGTGGCTACTATTTATGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGGTCATCATTCAACGATCGACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAttt         <  2:72489/141‑1 (MQ=11)
                                                                                                                                    tGTAGCGGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg        <  1:122744/141‑1 (MQ=255)
                                                                                                                                     gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg       <  1:25166/141‑1 (MQ=255)
                                                                                                                                     gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg       <  1:190284/141‑1 (MQ=255)
                                                                                                                                      tAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCAGCGATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga      <  1:10960/141‑1 (MQ=11)
                                                                                                                                      gaGCCGTTGGCTACTAACTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga      <  2:62405/140‑1 (MQ=255)
                                                                                                                                        gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag    <  2:29080/141‑1 (MQ=255)
                                                                                                                                         ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATCCAGCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga   >  1:298692/1‑141 (MQ=11)
                                                                                                                                         ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCCAATTTGgatga   >  1:100713/1‑141 (MQ=11)
                                                                                                                                          cGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTGCCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaagaa  >  1:87605/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                            
GCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAA  >  CP000730/76128‑76405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: