Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,295 T→C 61.8% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2950TC61.8% 287.1 / inf 468intergenic (+353/‑781)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base T (86/92);  new base C (146/142);  total (232/236)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 470 total aligned reads displayed.

TAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGC  >  CP000730/76157‑76433
                                                                                                                                          |                                                                                                                                          
           tcAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatatat                                                                                                                               <  1:245679/140‑1 (MQ=25)
            cAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatatatt                                                                                                                              >  2:15979/1‑140 (MQ=25)
            cAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc                                                                                                                              >  2:19955/1‑141 (MQ=35)
              aaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATCCATTGAGTTATCGTGATATATCTg                                                                                                                            >  2:152482/1‑141 (MQ=17)
               aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa                                                                                                                           <  1:237102/141‑1 (MQ=35)
                 ttGAATTTATAATATAATTGTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGaaa                                                                                                                         >  1:203031/1‑141 (MQ=255)
                  tgtatttataatataaTTTTAACAAAAAGGAGACTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGCTGTTTTCACTGTAGCAGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATAACTGAAat                                                                                                                        >  2:30686/7‑141 (MQ=1)
                       ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                                                   >  2:348209/2‑141 (MQ=9)
                       ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                                                   >  1:85132/2‑141 (MQ=12)
                       ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAAAATTaa                                                                                                                   >  1:158475/2‑141 (MQ=9)
                       ttataatataaTTTGAACAAAAAGGATTCCTCTGTATGAACTATTGCAGATATAAACAATTTAACAAGGATGTTAGCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                                                   <  1:172905/140‑1 (MQ=11)
                         ataCTATAATTTTAACAAAAAGGAGTCTGCTGTATGAACTATTTCAGATATAAACAATTTAAGATGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAga                                                                                                                 <  1:88940/141‑2 (MQ=11)
                          taatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa                                                                                                                   <  1:30250/135‑1 (MQ=16)
                          taatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTAGCCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAgag                                                                                                                >  2:210165/4‑139 (MQ=255)
                           agcgtaactatAACAAAAAGGAGTCTTCTGTACGAACTATTTCAGATATAAACAATCTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGCGTCATCGCGATATATCGGAAATATTAAGGGa                                                                                                               <  2:222613/131‑1 (MQ=11)
                                aaTTTTAACAAAAAGGAGTCCTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtg                                                                                                          <  2:327242/141‑1 (MQ=12)
                                     tAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                                                                                                     <  1:73397/141‑1 (MQ=32)
                                      aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTaa                                                                                                    <  1:5420/141‑1 (MQ=17)
                                      aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaa                                                                                                    <  1:186788/141‑1 (MQ=17)
                                      aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAAAGTGGGGTaa                                                                                                    >  2:185652/1‑141 (MQ=255)
                                       aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaaa                                                                                                   >  1:135373/1‑141 (MQ=17)
                                       aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaaa                                                                                                   >  1:178730/1‑141 (MQ=17)
                                        cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGTCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTTAAATATTAAGGGAAAGTGGTGTaacg                                                                                                  >  2:276850/1‑139 (MQ=255)
                                         aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTACCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTATGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACg                                                                                                 <  2:366799/141‑1 (MQ=255)
                                         aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTa                                                                                                     >  2:235318/1‑137 (MQ=18)
                                         aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGGACgcgagtgaaaat                                                                                                 >  2:171976/1‑130 (MQ=11)
                                         aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACg                                                                                                 <  1:77450/141‑1 (MQ=12)
                                          aaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGt                                                                                                >  1:182834/1‑141 (MQ=17)
                                            aaGGAGTCTTCTGTATGAATTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtc                                                                                              <  2:59783/141‑1 (MQ=255)
                                             aGGAGTCTTCTGTATAAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTTAAATATTAAGAGAACGTGGTGTAAACGTtca                                                                                             <  1:4418/141‑1 (MQ=255)
                                               gactcttatGTATGAACTATTTACAATACAAACAATTTAACCAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTGATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatc                                                                                           <  1:35060/138‑1 (MQ=11)
                                               gAGTCTTCTGTATGGACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatc                                                                                           <  2:164934/141‑1 (MQ=17)
                                                aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatca                                                                                          >  1:337075/1‑141 (MQ=12)
                                                 gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAAGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGGGTAAACGTTcatcat                                                                                         <  2:3346/141‑1 (MQ=12)
                                                   cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTc                                                                                       >  1:23244/1‑141 (MQ=17)
                                                   cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCCTCTTTc                                                                                       >  1:369177/1‑141 (MQ=255)
                                                    ttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCa                                                                                      <  1:11427/141‑1 (MQ=12)
                                                     tctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTAAGTTATCGTGCTATACCGGAAATATTAAGGGAAAGTGGTGTAAAAGTTCATCATTCaa                                                                                     >  2:42141/1‑141 (MQ=11)
                                                     tctGGATGAACTTTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCaa                                                                                     <  1:361459/141‑1 (MQ=17)
                                                      ctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGCTATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAAc                                                                                    <  1:220832/141‑1 (MQ=17)
                                                       tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACg                                                                                   >  2:120970/1‑141 (MQ=17)
                                                       tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACg                                                                                   >  1:286318/1‑141 (MQ=17)
                                                         tATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACgat                                                                                 <  2:337498/141‑3 (MQ=12)
                                                             aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTAc                                                                             >  1:48493/1‑141 (MQ=17)
                                                             aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGCAATATTAAGAGAACGTGGTGTAAACGTTCATCATTAAACGATCTAc                                                                             >  1:294185/1‑141 (MQ=11)
                                                             aaCTATTTCAGATATAAACAATTTAACAACGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGCTCATCATTCAACGGTCTAc                                                                             <  1:249108/141‑1 (MQ=12)
                                                              aCTATTTCAGACATAAAGAATTTAACAAGGATGTTATCACTGTAGCAGTTGGCTACTATCTAAGATATGCATTGAGCTATGGGGATATAACTGAACTAGTAAGAGAACGTGGTGGAAACGTTCATCATTCAACGATCTAcc                                                                            >  2:68452/1‑141 (MQ=11)
                                                                 aTTTCAGAGAGAAACAAGTGAACAAGGATGTTATCACTGGAGCCGTGGGCTACTATCTAAGAGATGCATGGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                         <  1:154794/141‑1 (MQ=11)
                                                                   ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATAATTCAACGATCTACCGGTgg                                                                       >  2:291023/1‑141 (MQ=11)
                                                                     cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGt                                                                     >  1:14753/1‑141 (MQ=12)
                                                                      aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                    >  2:287128/1‑141 (MQ=255)
                                                                      aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                    >  2:370330/1‑141 (MQ=255)
                                                                         tataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCaa                                                                 <  2:254810/141‑1 (MQ=17)
                                                                         tataAACAATTTAACAAGGATGTTACCACTGTAGCCGTTGGCTACTATCTAAGATTAGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCaa                                                                 <  1:239997/141‑1 (MQ=12)
                                                                          ataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                                                <  2:205130/141‑1 (MQ=17)
                                                                           taAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                               <  2:322259/141‑1 (MQ=17)
                                                                              aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAata                                                            >  1:278654/1‑141 (MQ=255)
                                                                              aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAata                                                            >  1:123937/1‑141 (MQ=255)
                                                                              aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAata                                                            >  1:143408/1‑141 (MQ=255)
                                                                              aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACCTTCATCATTCAACGATCTACCGTTGGGTTCAAGAata                                                            <  2:95099/141‑1 (MQ=255)
                                                                                  tttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                                                        >  1:324826/1‑141 (MQ=17)
                                                                                  tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                        >  2:135169/1‑141 (MQ=12)
                                                                                    tAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcccc                                                      >  1:131128/1‑141 (MQ=17)
                                                                                    tAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAGGATATGCATTGAGTTTTCGTGATATATCTGTAATATTAATGGAACGTGGTGTAGACGTTCATTATTCAGCGGTCTACCGGTGGGTTTAAGAATATGcctc                                                      >  2:354472/1‑139 (MQ=11)
                                                                                     aaCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCa                                                     <  1:213611/141‑1 (MQ=17)
                                                                                      aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTATACCGTTGGGTTCAAGAATATGCCCCaa                                                    >  1:265813/1‑141 (MQ=17)
                                                                                       cAAGGATGTTATCACTGGAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAt                                                   <  2:24281/141‑1 (MQ=255)
                                                                                         aGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAttt                                                 <  1:252868/141‑1 (MQ=17)
                                                                                           gATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAttttt                                               >  2:130915/1‑140 (MQ=255)
                                                                                              gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATAGGCCCCAATTTTatat                                            >  1:334616/1‑141 (MQ=11)
                                                                                              gTTATCACTGTAGCCGTTGACTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                            >  1:228692/1‑141 (MQ=11)
                                                                                               ttATCACTGTCGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGGGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                           <  2:22662/141‑1 (MQ=255)
                                                                                               ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCACttttgtgtt                                           >  1:117465/1‑136 (MQ=11)
                                                                                                tATCACTGTAGCTGGTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAGCGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                          <  2:179337/141‑1 (MQ=255)
                                                                                                tATCACTGTAGCCGTTGGCTACTATCTAAGATATGGATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTAAACGATCTACCGTTGGGTTCCAGAATCTGCTCAAATTCTTTATCa                                          <  2:378926/141‑1 (MQ=11)
                                                                                                   cACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCGAAt                                       >  2:268716/1‑141 (MQ=25)
                                                                                                   cACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCGTTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt                                       >  1:106066/1‑141 (MQ=255)
                                                                                                   cACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAAAGATGTGACGTTGAGTTCAAGAATATGCCCCAATTTTATATCAAAt                                       >  1:198174/1‑141 (MQ=11)
                                                                                                       gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                   >  1:99820/1‑141 (MQ=255)
                                                                                                        tAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                  <  1:35682/141‑1 (MQ=255)
                                                                                                        tAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCAGCGATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                  <  1:10960/141‑1 (MQ=11)
                                                                                                          gccactGGCGACTATCTAAGAGATGCATTTAGCTCACGTGACAAATCTCAAACATTAAGAGAACGAGTTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGACCCAATTTTATATCAAATTTGgaag                                <  2:80198/136‑1 (MQ=11)
                                                                                                           ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATCCAGCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                               >  1:298692/1‑141 (MQ=11)
                                                                                                           ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCCAATTTGgatga                               >  1:100713/1‑141 (MQ=11)
                                                                                                            tgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAGACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTGGgaagaa                              >  1:704/2‑141 (MQ=25)
                                                                                                               tGGCTAGTATCAAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaaa                           <  2:38541/141‑1 (MQ=11)
                                                                                                                 gCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGc                         <  1:208614/141‑1 (MQ=255)
                                                                                                                  ctactaTCTAAGATATGCATTGAGCAATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATCTCAAATTTGGAAGAAAAAGCa                        >  1:191822/1‑141 (MQ=11)
                                                                                                                   tactaTCTAAGATATGCATTGAGCTATCGGGATATATCTGAAATATTAACAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                       <  1:268587/141‑1 (MQ=11)
                                                                                                                    actaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGAATa                      >  1:379817/1‑141 (MQ=21)
                                                                                                                    actaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                      <  2:147966/141‑1 (MQ=255)
                                                                                                                     ctaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa                     <  1:316500/141‑1 (MQ=255)
                                                                                                                       attgaacatatGCATTGACCTAGTGTGATATATCTGAAAGATTAAGCGAACGTGGTGTAAACGTTCATCATTCAACGATGTACCAGGGGGTTCAAGAATATGCACCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                   <  2:143394/134‑1 (MQ=11)
                                                                                                                       aTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                   <  2:111141/141‑1 (MQ=35)
                                                                                                                       aTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                   <  2:49010/141‑1 (MQ=35)
                                                                                                                       aTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGACCGAAGTTTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGACGAAAAAGCATaaaa                   <  1:199964/141‑1 (MQ=11)
                                                                                                                          tAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaag                >  1:328003/1‑139 (MQ=18)
                                                                                                                           aaGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagc               <  1:367270/141‑4 (MQ=17)
                                                                                                                                atGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcctatt          >  1:57973/1‑133 (MQ=2)
                                                                                                                                        aGTTATCGTGATATACCTGAAATATTACGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGc  <  2:299625/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
TAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGC  >  CP000730/76157‑76433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: