BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	905,331	T→A	5.6%	I58I (ATT→ATA)	USA300HOU_0864 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	905,331	0	T	A	5.6%	399.0 / 5.6	133	I58I (ATT→ATA)	USA300HOU_0864	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (66/58); new base A (2/6); total (68/65)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

TATCTAACTTTGATGATATGGATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCACGTAAAGAAG  >  CP000730/905192‑905470

tATCTAACTTTGATGATATGGATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTa                                                                                                                                            <  2:247037/141‑1 (MQ=255)

tATCTAACTTTGATGATATGGATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTa                                                                                                                                            <  2:120096/141‑1 (MQ=255)

aatttatctgtgatgaAATGGATATTAAATGATATGCCAATCTATATCAAAAGGATAAAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGAAAAATa                                                                                                                                            <  2:297828/131‑1 (MQ=255)

   cTAACTTTGATGATATGGATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACa                                                                                                                                         <  2:160034/141‑1 (MQ=255)

          tgatgaTGTGAATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAatt                                                                                                                                  <  2:307895/141‑1 (MQ=255)

               atatGGATATTAAAGGAAAAGAAAAAATATATAAAAAGGATATAGGTAAAGATG‑TAAGTATGATCGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAAAAAAAAAAAAAAAAATTATTAg                                                                                                                             <  2:330530/141‑1 (MQ=255)

                tatGGATATTAAAGGATATGCCGATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAgg                                                                                                                            >  2:279552/1‑141 (MQ=255)

                 atGGATATTAAAGGAAAAGAAAATATATAACAAAAGGATATAGGTAAAGATG‑TAAGTATGAGTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTAACAAAAAAAAAAAAAAAAAAATTATTAGGa                                                                                                                           <  2:175860/141‑1 (MQ=255)

                 atGAATATTAAAGGATAGGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAATTTGTATTACCAGAGCAAATTAACAAAAAATTATTAGGa                                                                                                                           <  2:51444/141‑1 (MQ=255)

                   ggATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAgt                                                                                                                         <  1:137537/141‑1 (MQ=255)

                          aaaGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAat                                                                                                                  <  1:176933/141‑1 (MQ=255)

                          aaaGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAat                                                                                                                  <  2:209122/141‑1 (MQ=255)

                           aaGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAata                                                                                                                 >  2:35722/1‑141 (MQ=255)

                           aaGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGAGTTAAata                                                                                                                 >  2:100847/1‑141 (MQ=255)

                            aGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAAGAAAAAATTATTGGGAGTGTTAAatat                                                                                                                >  1:163251/1‑141 (MQ=255)

                            aGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTAAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAatat                                                                                                                <  1:375776/141‑1 (MQ=255)

                             ggATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATatg                                                                                                               <  2:151125/141‑1 (MQ=255)

                                  tGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATatgaatga                                                                                                          <  1:254022/141‑1 (MQ=255)

                                     cAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGt                                                                                                       >  1:34517/1‑141 (MQ=255)

                                      aaTCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTa                                                                                                      <  2:73410/141‑1 (MQ=255)

                                      aaTCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTa                                                                                                      >  1:237717/1‑141 (MQ=255)

                                       aTCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTaa                                                                                                     >  1:36682/1‑141 (MQ=255)

                                          tataTCAGAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAGTTAACAAAAAATTATTAGGAGTGTTAAAACTGAATGAAGTAaatc                                                                                                  >  1:239088/1‑141 (MQ=255)

                                             atCAAAAGGATATAGGTAACGATGATTCATAT‑ATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAaatcaat                                                                                               >  1:185259/1‑141 (MQ=255)

                                             atCAAAAGAATATAGGTAAAGGTG‑TAAGTATGAGTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACAAGAAAAAAAAAAAAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAaatcaat                                                                                               <  2:205050/141‑1 (MQ=255)

                                               cAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTaaaaaaaaca                                                                                             >  2:284474/1‑134 (MQ=255)

                                               cAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAatcaatca                                                                                             >  1:206043/1‑141 (MQ=255)

                                               cAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAACAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTGAAATATGAATGAAATAAatcaatca                                                                                             >  2:79142/1‑141 (MQ=255)

                                                aaaaGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTGTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAatcactcat                                                                                            >  2:284229/1‑140 (MQ=255)

                                                  aaGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCACATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGt                                                                                          <  1:247545/141‑1 (MQ=255)

                                                  aaGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGt                                                                                          <  2:349717/141‑1 (MQ=255)

                                                   gggATATAGGTAAAGCCG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTa                                                                                         >  2:83942/2‑141 (MQ=255)

                                                    ggATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTCAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAACTTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTaa                                                                                        >  1:51261/1‑141 (MQ=255)

                                                    ggATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAATGTTAAATATGAATGAAGTAAAACAATCAAGTaa                                                                                        >  1:235321/1‑141 (MQ=255)

                                                     gATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAAt                                                                                       >  1:138841/1‑141 (MQ=255)

                                                      atatAGGTAAAGATG‑TAAGTATGATTGAACAGGCGTTAAAGCAAGTAAGTATTAATGAAACAGAAGTTTTATTACCAGAGCAAATTAAAAAAAAATTAGTAGGAGTGTTAAATAAGAATGAAGTAAATCAATAACGTAATa                                                                                      >  2:163309/1‑141 (MQ=255)

                                                          aGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAATCAAATAAGTATTACTGAAACAGAAGTTTTATAACCAGAGCAAATTAACAAAAAATTTTTAGGAGTGTGAAATATGAATGAAGTAAATCAATCAAGTAAGACATg                                                                                  <  2:68492/141‑1 (MQ=255)

                                                           ggTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATgg                                                                                 <  2:293022/141‑1 (MQ=255)

                                                           ggTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTACTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGtcacccatgt                                                                                 <  2:45846/141‑10 (MQ=255)

                                                            gTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAACAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATggg                                                                                <  2:81048/141‑1 (MQ=255)

                                                             gaaaGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGggt                                                                               <  1:132618/140‑1 (MQ=255)

                                                              aaaGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGggta                                                                              <  2:188715/141‑1 (MQ=255)

                                                                   tG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAACAATTATTAGAAGTGTTAAATCTGAATGCAGTACATCAATCAAGTAATACATGGGTAGGTAc                                                                         >  1:336249/1‑141 (MQ=255)

                                                                   tG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTAc                                                                         >  1:360336/1‑141 (MQ=255)

                                                                    g‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTAcc                                                                        >  1:357684/1‑141 (MQ=255)

                                                                    g‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTAcc                                                                        >  1:116275/1‑141 (MQ=255)

                                                                      tAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCt                                                                       <  2:217863/141‑1 (MQ=255)

                                                                       aaGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCtt                                                                      >  1:85869/1‑141 (MQ=255)

                                                                         gTATGATTGAACAGGGGCTAAAGCAACTAACGATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaa                                                                    >  2:159001/1‑141 (MQ=255)

                                                                            tGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaacaa                                                                 >  1:315932/1‑141 (MQ=255)

                                                                            tGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaacaa                                                                 >  1:83017/1‑141 (MQ=255)

                                                                               ttGAACAGGGGTTAAATCAATGAAGTATTACTGAAACAGAAGTTTTATTACCAGCGCAAATTAACAAAAAATTATTCGGTGTGTTAAATATGAATGAAGTAAATCAATTCAGTAATACATGGGTACGCACCGTaacaaaac                                                              <  2:289425/141‑1 (MQ=255)

                                                                               ttGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaacaaaac                                                              <  2:133464/141‑1 (MQ=255)

                                                                               ttGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaacaaaac                                                              <  1:125732/141‑1 (MQ=255)

                                                                               ttGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaacaaaac                                                              <  2:177176/141‑1 (MQ=255)

                                                                               tggAACAGGTGTTAAAACAATCAAATATTAATGAAACAGAAGTTTTATTACCAAAGAAAAAAAAAAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTaacaaaac                                                              <  2:184255/139‑1 (MQ=255)

                                                                                   aCAGGGGTTAAAGCAATTAAGTATTACTTAAACAGAAGTTTTAGTACCAGAGCAAATTAACAAACAATTATTAGCAGAGTGAAATATGAATGAAGTAAATCAATCAAGGAATACATGGGTAAGTACCTTaacaaaacaagg                                                          >  1:286260/1‑139 (MQ=255)

                                                                                   aCAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAAtt                                                          >  1:110382/1‑141 (MQ=255)

                                                                                   aCAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAAtt                                                          >  1:60794/1‑141 (MQ=255)

                                                                                      ggggTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGCGTGGGCGATAGGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAAttagt                                                       >  1:182212/1‑141 (MQ=255)

                                                                                      ggggTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAAttagt                                                       <  1:332955/141‑1 (MQ=255)

                                                                                        ggTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAAttagtta                                                     >  2:360680/1‑141 (MQ=255)

                                                                                           tAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTtagta                                                  <  2:264785/141‑1 (MQ=255)

                                                                                           tAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTtagta                                                  >  1:361380/1‑141 (MQ=255)

                                                                                              aGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTg                                               <  1:291701/141‑1 (MQ=255)

                                                                                               gCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACCTGGGTAGGTACCTTAACAGAACAACTAGTTAGTAGTgg                                              >  2:301253/1‑140 (MQ=255)

                                                                                               gCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTga                                              <  2:328144/141‑1 (MQ=255)

                                                                                                 aaTTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTgaga                                            <  1:54945/141‑1 (MQ=255)

                                                                                                     aaGTATTACTGAAACAGAAGTTTTATTACGCGAAGCAGTAAAGAGAAAATTACTAAGGTATTTAAATATGACGGAAGTAAATCAATCAAGTAATACAGGGGGAGGTACCTTGAGCAGACAATTGGTTAGTAGTGAGAATaa                                        >  1:183700/1‑141 (MQ=255)

                                                                                                       gTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACt                                      <  1:226836/141‑1 (MQ=255)

                                                                                                           tACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAACCAATTAGTTAGTAGTGAGAATAACTTTaa                                  <  2:178732/141‑1 (MQ=255)

                                                                                                           tACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTaa                                  <  2:63679/141‑1 (MQ=255)

                                                                                                               gAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATAtt                              <  2:217053/141‑1 (MQ=255)

                                                                                                               gAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATAtt                              >  2:241329/1‑141 (MQ=255)

                                                                                                               gAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATAtt                              >  2:35144/1‑141 (MQ=255)

                                                                                                                aaaCAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACCATTAGTTAGTAGTGAGAATAACTTTAATAtta                             >  1:256667/1‑140 (MQ=255)

                                                                                                                 aaCAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAatatcaa                            >  2:93813/1‑138 (MQ=255)

                                                                                                                   cAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAag                          <  1:344228/141‑1 (MQ=255)

                                                                                                                   cAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAag                          >  1:60529/1‑141 (MQ=255)

                                                                                                                    aGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAaga                         >  2:292993/1‑141 (MQ=255)

                                                                                                                       aGTTTTATTACCAGAGCAAATTCACAAAAAATTACTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAACACACTTAGTTAGTCGTGAGAATAACTTTAATATTCAAGAGtt                      >  2:340272/1‑141 (MQ=255)

                                                                                                                        ttcttactaCCAGAGCAAATTAACAAAAAATTATTCGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTa                     <  1:79142/138‑1 (MQ=255)

                                                                                                                        gTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTa                     <  1:284474/141‑1 (MQ=255)

                                                                                                                        gTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTa                     <  2:206043/141‑1 (MQ=255)

                                                                                                                        gTTTTATGACCAAAGCAAATGAACAAAAAATTATTAGGAGGATTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTa                     <  1:366271/141‑1 (MQ=255)

                                                                                                                          tttATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTAcc                   <  2:369529/141‑1 (MQ=255)

                                                                                                                          tttATTACCAGACCAAACTAACAAAAAATTATTAGGCGTGTTAAATATGAATGAAGTAAACCAATCAACTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTAcc                   <  1:15739/141‑1 (MQ=255)

                                                                                                                          tttATTACCAAAAAAAAAAAAAAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTAcc                   <  2:376396/141‑1 (MQ=255)

                                                                                                                              ttaCCAGAGCAAATTAACACAAAATTATTAGGAGTGGTGAATATGAATGAAGTAAATCAATCAAGTAATAAATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGACTAACTTTAATCAGCAGGAGCTGCCAACt               >  2:104603/1‑141 (MQ=255)

                                                                                                                              ttaCCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCaaat               >  1:188974/1‑139 (MQ=255)

                                                                                                                              ttaCCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACt               <  2:361380/141‑1 (MQ=255)

                                                                                                                               tcccAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTg              <  1:346832/139‑1 (MQ=255)

                                                                                                                               taCCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTg              <  2:362736/141‑1 (MQ=255)

                                                                                                                                 ccAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCa            >  1:261699/1‑141 (MQ=255)

                                                                                                                                      gCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAAAAACTTTAATATTCAAGAGTTACCAACTGCACGTaa       <  2:365408/141‑1 (MQ=255)

                                                                                                                                       cAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAGGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCACGTaaa      >  1:254764/1‑141 (MQ=255)

                                                                                                                                        aaaTTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTACATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCACGTAaag     >  2:204619/1‑141 (MQ=255)

                                                                                                                                         aaTTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCACGTAaaga    >  2:90444/1‑141 (MQ=255)

                                                                                                                                          aTTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCACGTAaagaa   <  1:99635/141‑1 (MQ=255)

                                                                                                                                           ttAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCACGTAaagaag  >  1:62509/1‑141 (MQ=255)

TATCTAACTTTGATGATATGGATATTAAAGGATATGCCAATCTATATCAAAAGGATATAGGTAAAGATG‑TAAGTATGATTGAACAGGGGTTAAAGCAATTAAGTATTACTGAAACAGAAGTTTTATTACCAGAGCAAATTAACAAAAAATTATTAGGAGTGTTAAATATGAATGAAGTAAATCAATCAAGTAATACATGGGTAGGTACCTTAACAAAACAATTAGTTAGTAGTGAGAATAACTTTAATATTCAAGAGTTACCAACTGCACGTAAAGAAG  >  CP000730/905192‑905470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`