Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,232,465 G→C 100% A124P (GCG→CCG)  prkC → non‑specific serine/threonine protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,232,4650GC100.0% 110.9 / ‑6.5 34A124P (GCG→CCG) prkCnon‑specific serine/threonine protein kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (10/23);  minor base A (0/1);  total (10/24)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAGAAGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCATGTG  >  CP000730/1232328‑1232602
                                                                                                                                         |                                                                                                                                         
aagaagAGGACTGTTACTACTTAGGAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATccgc                                                                                                                                        <  1:127737/141‑1 (MQ=255)
  gaagaTGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCAt                                                                                                                                      <  2:127571/141‑1 (MQ=255)
  gaagaTGACTGTAACTACTTAGTAATGAAAAATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCAt                                                                                                                                      <  1:137859/141‑1 (MQ=255)
        taCTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATg                                                                                                                                <  1:328526/140‑1 (MQ=255)
        gACCGTTACTATGTAGTAATGGAATATATCGAAGGCCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATg                                                                                                                                <  2:107543/141‑1 (MQ=255)
         aCTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGc                                                                                                                               <  1:2591/141‑1 (MQ=255)
         aCTGGTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGc                                                                                                                               <  2:72680/141‑1 (MQ=255)
          cTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGACTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCg                                                                                                                              <  2:270858/141‑1 (MQ=255)
                ctactTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGt                                                                                                                        <  2:237293/141‑1 (MQ=255)
                ctactTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCAGGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCGTGATATGCGTATTGt                                                                                                                        <  2:108988/141‑1 (MQ=255)
                 tacgTAGTAATGGAATATATCGAAGGTCCGACTGTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTa                                                                                                                       <  2:222260/141‑1 (MQ=255)
                          aTGGAATATATCGAAGGACCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGat                                                                                                              <  1:227712/141‑1 (MQ=255)
                                                cTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTaa                                                                                        >  2:242964/1‑141 (MQ=255)
                                                               ttGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATaaaa                                                                         >  2:237677/1‑141 (MQ=255)
                                                               ttGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATaaaa                                                                         >  2:131117/1‑141 (MQ=255)
                                                                              cATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTg                                                          <  2:5057/141‑1 (MQ=255)
                                                                                   agggtagagactgCGAATAATTTAACGAATCAAATATTGGATGGCATTAAACATCAGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGAtttt                                                     <  2:74011/129‑1 (MQ=255)
                                                                                   aGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGAtttt                                                     <  2:352908/141‑1 (MQ=255)
                                                                                   aGTGTGGAAAAAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGACACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGAtttt                                                     <  2:288882/141‑1 (MQ=255)
                                                                                     tgtTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTGGATTTTgg                                                   >  1:238055/1‑141 (MQ=255)
                                                                                     tgtTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAAAATTTGATTTTgg                                                   >  1:55131/1‑141 (MQ=255)
                                                                                         gACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAAtt                                               <  1:81870/141‑1 (MQ=255)
                                                                                              aGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTaa                                          >  2:61412/1‑141 (MQ=255)
                                                                                                    tAAGTTTACGAATCAAATTTTGGATGGAATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCgtt                                    <  2:192989/141‑1 (MQ=255)
                                                                                                      aTTTTAAGAATCAAATAATGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGAGATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTaa                                  <  1:307592/141‑1 (MQ=255)
                                                                                                          taagAAACAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGtta                              <  2:81795/138‑3 (MQ=255)
                                                                                                          tACGAATCAAATATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTga                              <  1:225452/141‑1 (MQ=255)
                                                                                                                  aaaTATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCtt                      <  1:129521/141‑1 (MQ=255)
                                                                                                                  aaaTATTGGATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGAAATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCtt                      <  1:39582/141‑1 (MQ=255)
                                                                                                                  aaaTAGTGGATGGCATTAAACATACGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCtt                      <  2:300893/141‑1 (MQ=255)
                                                                                                                         ggATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCa               >  2:375685/1‑141 (MQ=255)
                                                                                                                         ggATGGCATTAAACATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGGGAGACGTCTTTAACTCa               >  2:1907/1‑141 (MQ=255)
                                                                                                                                   aaaCATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCAt     >  2:252511/1‑141 (MQ=255)
                                                                                                                                   aaaCATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCAt     >  2:182285/1‑141 (MQ=255)
                                                                                                                                      cATCCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGCGACGTCTTTAACTCAGACTAATCAtgtg  <  1:242964/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
AAGAAGATGACTGTTACTACTTAGTAATGGAATATATCGAAGGTCCGACTTTGTCTGAGTATATTGAAAGTCATGGGCCATTAAGTGTTGACACAGCGATTAATTTTACGAATCAAATATTGGATGGCATTAAACATGCGCATGATATGCGTATTGTACATAGAGATATTAAGCCACAAAATATATTAATTGACAGCAATAAAACGTTGAAAATATTTGATTTTGGAATTGCTAAAGCTTTAAGTGAGACGTCTTTAACTCAGACTAATCATGTG  >  CP000730/1232328‑1232602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: