| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,277,850 | A→G | 11.4% | intergenic (+448/‑498) | pyrH → / → uppS | UMP kinase/di‑trans, poly‑cis‑decaprenylcistransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,277,850 | 0 | A | G | 11.4% | 79.2 / 4.2 | 36 | intergenic (+448/‑498) | pyrH/uppS | UMP kinase/di‑trans, poly‑cis‑decaprenylcistransferase |
| Reads supporting (aligned to +/- strand): ref base A (15/16); new base G (2/2); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01 | |||||||||||
CCTGCCTTAACAGAAGAACGTAGAAAAGAGCGCGTTAAAGATGTTAAGAAAATTGGTGAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGATTGCTGATAAAGAAAAAGATATTATGTCAGTATAAAACTAATATACAATG > CP000730/1277724‑1277990 | ccTGCCTTAACAGAAGAACGTAGAAAAGTGCGCGTTAAAGATGTTAAGAAAATTGGTGAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGTGATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACt > 1:98304/1‑141 (MQ=255) gCCTTAACAGAAGAACGTAGAAAAGAGCGCGTTAAAGATGTTAAGAAAATTGGTGAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACGGaa > 2:244600/1‑141 (MQ=255) cTTAACAGAAGAACGAAGAAAAGAGGGCGTTAAAGATGTTAAGAAAATTGGTGAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGa < 2:103055/141‑1 (MQ=255) aaCAGCAGAACGTAGAAAAGAGCGCGTTAAAGATGTTAACAAAATTGGTGAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGAACAGTTGAAAACAGATGAAAAAAATGGCGACATTACTGAAGATGa > 2:81603/1‑141 (MQ=255) aaCAGAAGACCGTAGCCACGAGCGCGTCACAGCTGTTAAGAAAATCGGTGAAGCAGCTAAAGTATCTGTTCGAAATCTTCGTCGTGATCTGACTGATCAGTTGAAACAAGCTGCAACCAATGGCCACATCACTGACGCTGa > 2:349622/1‑141 (MQ=255) aaCAGAAGAACGTAGAAAAGAGCGCGTTAAAGATGTTAAGAAAATTGGTGACGAAGCTAAAGTATCTGTTAGAAAAATTCGTCGTGATATGAATGCTAAGTTGAACAAAGATGAAAAAAATGGCGACATTACTGAAGATGa > 2:168444/1‑141 (MQ=255) cAGAAGAACGTAGAAAAGAGCGCGTTAAAGATGTTAAGAAAATTGGTGAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGaggcgg > 1:263153/1‑136 (MQ=255) agaaCGTAGAAAAGAGCGCGTTAAAGATGTTAAGAAAATGGGTGAAGAAGCTAAAGTATCGGTTCGAAATATTCATCCTGATATGAATGATCAGTTGAAAAAAGATGAAAAAACTGGACACATTACTGAAGAGGAGATgac > 1:135857/1‑140 (MQ=255) agaaCGTAGAAAAGAGCGCGATAAAGATGTTAAGAAAATTGGTGAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGAGCAGGTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTgag < 1:32804/141‑1 (MQ=255) cGTAGAAAAGGGCGCTGTAAAGATGTTAAGAAAATTGGTGAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGt < 1:313664/141‑1 (MQ=255) cGTAGAAAAGGGCGCGTTTCTAGTGGTAATGAAATTGGTGAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGGAGATGAGTTGAGAAGt < 2:172130/141‑1 (MQ=255) cGTAGAAAAGAGCGCGTTAAAGATGTTAAGAAAATTGGTGAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGt < 1:34594/141‑1 (MQ=255) aGAAAAGAGCGCGTTAAAGATGTTAAGAAAATTGGTGAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAGATGGCGACATTACTGAAGATGAGTTGAGAAGTGGt > 1:305050/1‑141 (MQ=255) aGAAAAGAGCGCGTTAAAGATGTTAAGAAAATTGGTGAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAGATGGCGACATTACTGAAGATGAGTTGAGAAGTGGt > 1:208520/1‑141 (MQ=255) gagCGCGTTAAAGATGTTAAGAAAATTGGTGAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGaa < 2:137376/141‑1 (MQ=255) tAAGAAAGTTGGTGAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAAc < 2:90280/141‑1 (MQ=255) gaagCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATaaaa > 1:149039/1‑141 (MQ=255) aagCGAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGAGCAGTTGAAAAAAGATGAACAATCTGGCGACACTACCGAAGATTAGTTGAGATGTGGTACTGAAGATGTTCACAAAGCAACAGACAATTCAATAAAAg < 2:217/141‑1 (MQ=255) cTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGaaa > 1:77978/1‑141 (MQ=255) cTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTGGCGAGGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGaaa > 1:106115/1‑141 (MQ=255) aGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGa < 1:122772/141‑1 (MQ=255) ggTTCGAAATATTCGTCGTGGTATGAATGATAGGTGGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAAt < 2:5538/140‑1 (MQ=255) atatACGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGATTGCTg > 2:264965/1‑141 (MQ=255) tcgtGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGATTGCTGATAAAGa < 1:346896/141‑1 (MQ=255) cccggATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGACTGCTGATAAAGa < 1:196253/137‑1 (MQ=255) gtGATATGAATGATCAGTTGAAAAAAGATGAAAAAGATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGATTGCTGATAAAGaaa < 2:208520/141‑1 (MQ=255) gtGATATGAATGATCAGTTGAAAAAAGATGAAAAAGATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGATTGCTGATAAAGaaa < 2:305050/141‑1 (MQ=255) atgaatgaTCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGACTGCTGATAAAGAAAAAGat < 1:210092/141‑1 (MQ=255) ttaatgaTCAGTTGAAAAAAAATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGATTGCTGATAAAGAAAAAGata < 2:51059/139‑1 (MQ=255) tgaatgcTCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCGATAAAAGCAATTGATCAACTGCTTGCTCATAAAGAAAAAGata > 2:323023/1‑141 (MQ=255) gaatgaTCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGATTGCTGATAAAGAAAAAGatat < 1:293859/141‑1 (MQ=255) tCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGATTGCTGATAAAGAAAAAGATATTATGTc < 1:18687/141‑1 (MQ=255) aGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGATTGCTGATAAAGAAAAAGATATTATGTCAg < 1:109096/141‑1 (MQ=255) aaGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGATTGCTGATACAGAAAAAGATATTATGTCAGTATAAAACt > 2:78301/1‑141 (MQ=255) aGATTAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGATTGCTGATAAAGAAAAAGATATTATTTCAGCATAAAACTa > 2:65162/1‑141 (MQ=255) gAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGATTGCTGATAAAGAAAAAGATATTATGTCAGTATAAAACTAatat > 1:237112/1‑141 (MQ=255) aaTGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGATTGCTGATAAAGAAAAAGATATTATGTCAGTATAAAACTAATATACAAta > 2:377641/1‑140 (MQ=255) | CCTGCCTTAACAGAAGAACGTAGAAAAGAGCGCGTTAAAGATGTTAAGAAAATTGGTGAAGAAGCTAAAGTATCTGTTCGAAATATTCGTCGTGATATGAATGATCAGTTGAAAAAAGATGAAAAAAATGGCGACATTACTGAAGATGAGTTGAGAAGTGGTACTGAAGATGTTCAGAAAGCAACAGACAATTCAATAAAAGAAATTGATCAAATGATTGCTGATAAAGAAAAAGATATTATGTCAGTATAAAACTAATATACAATG > CP000730/1277724‑1277990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |