Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,797,772 G→C 100% R7G (CGC→GGC)  phoR ← sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,797,7720GC100.0% 74.2 / NA 25R7G (CGC→GGC) phoRsensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (16/9);  total (16/9)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAAATATATGATCTTGCATCTGTTGCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCGGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACATCA  >  CP000730/1797636‑1797901
                                                                                                                                        |                                                                                                                                 
taaatcTATGATCTTGCATCTGTTGCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCcgtgg                                                                                                                               <  1:76612/141‑1 (MQ=255)
     ttattgtCTTGCATCTGTTGCTAGAGCTTGGTCGTGTATTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGaaa                                                                                                                          <  2:57783/135‑1 (MQ=255)
       atGATCTTGCATCTGTTGCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACt                                                                                                                        >  1:186780/1‑141 (MQ=255)
            cTTGCATCTGTTTCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTtcatc                                                                                                                   <  2:376775/141‑1 (MQ=255)
               gCATCTGTTGCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCAtt                                                                                                                >  1:239011/1‑141 (MQ=255)
                cATCTGTTGCTAGAGCTTGTTCGTGTTTTTCTGATCAAACATATGTGCTGGAAGCGTGAATAATAGTGCCTAAGGTAACAAAACTGATAATTAATCTACTGCTTTTCTATAACATTAAGCCGTGGTGAGACTTGATCAttt                                                                                                               >  1:343600/1‑140 (MQ=255)
                         cTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAATCTTAAGCCGTGGTGAAACTTCATCATTCTTCTTTAgg                                                                                                      >  2:262259/1‑141 (MQ=255)
                                    tCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAAtt                                                                                           >  1:147858/1‑141 (MQ=255)
                                     cGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTTTTTCCAAttt                                                                                          >  2:254302/1‑141 (MQ=255)
                                     cGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACAATAAGCCGGGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAAttt                                                                                          >  1:164438/1‑141 (MQ=255)
                                        gtTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTtata                                                                                       >  1:244803/1‑141 (MQ=255)
                                               tgttAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAg                                                                                <  1:20785/138‑1 (MQ=255)
                                                   aaaaCATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCAc                                                                            <  1:225387/141‑1 (MQ=255)
                                                     aaCATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGGTAAGTAATATACTGCTTATCAATAACATTAAGCCGTGGAGAAACTCCATCAGTGTTCTTCAGGCATTTCCAATTTATAGACTAAGcaagcc                                                                          >  2:11549/1‑136 (MQ=255)
                                                        atatGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTTTAGCCTAAGCCACGcaca                                                                       >  1:76343/1‑141 (MQ=255)
                                                         tatCTGAGGGAAGCGAGGACAATAAGGCCTAATGTAACATAACTGAAAAATAATTTGCTACGGATAATTTACATTTAGCCGGTTTTTAACTTCAGCATTGTTCTTTAGGTCTTTCCGATTTATAGCCTAAGCCACGCACAg                                                                      <  2:62743/141‑1 (MQ=255)
                                                                                               aaatCTGATAATTAATATGCTGCTTTGCAATAACATTAAGCCGTGGGTAAACTTCATCCTTGTTCTTTAGGTCTTTCTAATTTATAGCCTAAGCTACGCACAGTTTTAATAAGGTGTGGCTTCTTAGGATTATCTTCTAAt                                <  2:44139/141‑1 (MQ=255)
                                                                                               aaaaCTGATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAACCCACTCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAAt                                <  2:80938/141‑1 (MQ=255)
                                                                                                     gATAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTAtct                          >  2:193952/1‑141 (MQ=255)
                                                                                                      aTAATTAATATACTGCTTATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTAtctc                         >  2:158200/1‑141 (MQ=255)
                                                                                                              tataCTGCTTATAAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTATGATTATGTTCTAATTTATCTTTTAAATGa                 >  2:172825/1‑141 (MQ=255)
                                                                                                                      ttATCAATAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATg         >  2:288448/1‑141 (MQ=255)
                                                                                                                       tATCAATAACATTAAGCCGTGGTGAAACTTCATCATCGTTCTTTAGGTCTTTCCTATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATATCTTAAATGACTGATATGt        <  1:213816/141‑1 (MQ=255)
                                                                                                                            aTAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTCCATc   >  2:380747/1‑141 (MQ=255)
                                                                                                                            aTAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATAACTGAAATGTACATc   >  2:215055/1‑141 (MQ=255)
                                                                                                                             tAACATTAAGCCGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACATca  <  2:186780/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                 
TAAATATATGATCTTGCATCTGTTGCTAGAGCTTGTTCGTGTTTTTCTGATAAAACATATGTGATGGAAGCGTGAATAATAATGCCTAATGTAACAAAACTGATAATTAATATACTGCTTATCAATAACATTAAGCGGTGGTGAAACTTCATCATTGTTCTTTAGGTCTTTCCAATTTATAGCCTAAGCCACGCACAGTTTTAATAAGTTGTGGCTTCTTAGGATTATCTTCTAATTTATCTCTTAAATGACTGATATGTACATCA  >  CP000730/1797636‑1797901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: