BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,913	C→G	24.2%	S213S (TCG→TCC)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,913	0	C	G	24.2%	356.2 / 168.3	259	S213S (TCG→TCC)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (76/119); new base G (27/35); total (103/156)
Fisher's exact test for biased strand distribution p-value = 5.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 261 total aligned reads displayed.

GAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTC  >  CP000730/35776‑36053

gaacaaagagtaaaataaaaTTGTAATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaa                                                                                                                                           <  2:303541/131‑1 (MQ=255)

      gagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCg                                                                                                                                     >  2:259048/1‑141 (MQ=255)

       agCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGt                                                                                                                                    <  2:26462/141‑1 (MQ=255)

              acaaaagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                                                                                           <  1:3457/127‑1 (MQ=11)

               caaaagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                                                          <  1:54206/128‑1 (MQ=11)

               caaaagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATAACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                                                          >  1:230165/14‑141 (MQ=11)

                   agagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATTTATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                                                      <  1:50098/132‑1 (MQ=11)

                    gagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                                                     <  1:361002/133‑1 (MQ=11)

                      ttgATTTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                                                     <  2:37991/140‑1 (MQ=255)

                       tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGATCTGAAGAGACCTGc                                                                                                                  <  2:192795/136‑1 (MQ=11)

                        taTCTAATAAAAATAGAAGGGTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATGTATCATGTCAGTGTTCGCTTAACTTGCTAGCATTATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                   <  1:364514/140‑1 (MQ=255)

                          tttaataaAAAT‑TATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTCATATATCATGTCAGTGTTCGTTTAACCTGCTAGTTTGATGCTAATTTCGTGGTATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                                                               <  1:174426/139‑1 (MQ=11)

                          tttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATACAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                                                               <  2:208841/139‑1 (MQ=11)

                             aataaAAATGGAAGTTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTGATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                                                              <  2:182580/141‑1 (MQ=255)

                               taaAAATAGAAGGTTCTGTTGCAAAGTAAACAAATATAGCTAACCACTAATTTATCACGTCCGTGTTCGCTTAACTTGCTAGCAGGAAGCTAATTTCGTGGCAGGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGGTCt                                                                                                            >  2:39149/1‑141 (MQ=11)

                                    aTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAGCTGCGGTTCTTTTta                                                                                                       <  1:284926/141‑1 (MQ=18)

                                     ccgAAGTTTCTTTTGCAAAGT‑AAAAAATATAGCTACTCACTAATATATCATGTCAGTGTTCGTTTAGCTTGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata                                                                                                     <  2:284489/139‑1 (MQ=11)

                                      aGAAGGTTCTGTGGCAAAGTAAAAAAATATAGCTAACAACTGATTTATCATATCAGTGTTCGATTAACTTACTAGCATGATGCTAATTCCGTGGCATGGCGAAAGTCCGTAGATCTGAAGAGACCTGCGGTGCttttgaga                                                                                                     >  2:95131/1‑137 (MQ=11)

                                        aaGTTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCCAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtatata                                                                                                   <  2:256738/141‑1 (MQ=11)

                                                                      ccaatcACTAATATTGCATGGCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAg                                                                     <  1:10667/136‑1 (MQ=1)

                                                                                atatATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGCGACCTACGGTTCTATTGAGAGAACGCGTAAATAAATCCAATCCCTTTTAACCTATCCTTTGc                                                           >  2:79680/4‑141 (MQ=1)

                                                                                           ccgtggccGCTTAACGTGCTAGCATGATGCTAATTTCGTGTCCTGGCGAAAATCCCTAGATCTGAAGAGACCTGCGGTTCTTGTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                <  1:79214/134‑1 (MQ=11)

                                                                                           cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                <  1:318721/141‑1 (MQ=11)

                                                                                           cAGTGTTCGCTTAACCTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                <  1:134009/141‑1 (MQ=11)

                                                                                           cAGCGTTCGTTTAACTTGCGAGCATGATGCTCATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                <  1:76648/141‑1 (MQ=11)

                                                                                            cgctgtCGCCTGACTTGCTTGTATCATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCGTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                               <  2:154066/136‑1 (MQ=11)

                                                                                            aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt                                               >  2:140256/1‑141 (MQ=255)

                                                                                            aGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGCAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                               <  2:54776/141‑1 (MQ=9)

                                                                                            aGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                               <  2:223220/141‑1 (MQ=31)

                                                                                             gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                              >  1:335882/1‑141 (MQ=17)

                                                                                             gggtTCGTTTACTTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTACATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                              <  1:241656/139‑1 (MQ=11)

                                                                                             gggtTCGTTTAACTTGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                              <  2:35393/139‑1 (MQ=12)

                                                                                              tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                             >  2:193922/1‑141 (MQ=255)

                                                                                              tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTAAATACCTTTTAAAGTATTATTAGCCGTATTGATACttt                                             >  1:294121/1‑141 (MQ=11)

                                                                                              tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                             >  1:283160/1‑141 (MQ=12)

                                                                                               gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg                                            >  1:238530/1‑141 (MQ=255)

                                                                                                ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa                                           <  2:299028/141‑1 (MQ=255)

                                                                                                ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa                                           <  1:371890/141‑1 (MQ=255)

                                                                                                ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                           <  1:315140/141‑1 (MQ=12)

                                                                                                ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                           <  2:319597/141‑1 (MQ=32)

                                                                                                ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAGTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                           >  1:41949/1‑141 (MQ=17)

                                                                                                ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                           <  2:17873/141‑1 (MQ=17)

                                                                                                 tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                          <  1:304553/141‑1 (MQ=32)

                                                                                                    tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                       <  2:198324/140‑3 (MQ=255)

                                                                                                      tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                     >  1:159625/1‑141 (MQ=17)

                                                                                                      tAACCTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCtt                                     <  2:147689/141‑1 (MQ=11)

                                                                                                         cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                  <  2:338003/141‑1 (MQ=17)

                                                                                                          ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                 <  2:131299/141‑1 (MQ=17)

                                                                                                          ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                 <  1:6287/141‑1 (MQ=17)

                                                                                                           tGCTAGCATGATGCTATCTTCGGGGTATGGCGAAAATCCGCAGATCTGAAGAGACCTGCGCTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                <  2:274160/141‑1 (MQ=11)

                                                                                                           tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                <  1:220131/141‑1 (MQ=17)

                                                                                                            gCTAGCATGATGCTAATTTCGTGGCATGGCGGATTGCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                               >  1:378593/1‑141 (MQ=11)

                                                                                                            gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTTTTTGGATACTTTGATACCTTGtcttt                               <  1:281280/141‑1 (MQ=255)

                                                                                                            gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGCTACTTTGATACCTTGtcttt                               >  2:208196/1‑141 (MQ=17)

                                                                                                            gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                               >  1:121436/1‑141 (MQ=17)

                                                                                                            gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                               >  1:215240/1‑141 (MQ=17)

                                                                                                             cTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttt                              >  1:225774/1‑140 (MQ=255)

                                                                                                             cTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttc                              <  1:120339/141‑1 (MQ=11)

                                                                                                             cTAGCATGATGCTAATTCCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttc                              <  1:102238/141‑1 (MQ=11)

                                                                                                              tAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                             <  1:27598/141‑1 (MQ=11)

                                                                                                              gaGCAGGATGCTAATTTCGTGGCATGGGGAAAAACCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                             <  2:77237/140‑1 (MQ=11)

                                                                                                                gCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTa                           >  1:355797/1‑141 (MQ=11)

                                                                                                                gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttcttc                           >  2:350164/1‑140 (MQ=17)

                                                                                                                gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                           >  2:314249/1‑141 (MQ=17)

                                                                                                                 cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTAc                          >  1:141545/1‑141 (MQ=17)

                                                                                                                  atgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACt                         >  1:205295/1‑141 (MQ=18)

                                                                                                                   tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACGTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                        >  2:62959/1‑141 (MQ=11)

                                                                                                                    gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAACGTATTCTGTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                       <  1:168274/141‑1 (MQ=25)

                                                                                                                    gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                       <  1:242723/141‑1 (MQ=25)

                                                                                                                     atgCTAATTTCGTTGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTGTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTa                      <  2:329497/141‑1 (MQ=11)

                                                                                                                       gCTAATTTCGTGGCATGGGGAAAATCCTGAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAat                    <  1:179682/141‑1 (MQ=11)

                                                                                                                       gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                    >  2:100025/1‑141 (MQ=35)

                                                                                                                        cTAATTTGGTGGCATGGCGAAAATCCGTACATCTGAAGCTACCTGCGGTTCTTTTTATATAGAGCGTCAACACACTCAATACCTTTTGAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTCCTTACTTTAata                   <  2:285808/141‑1 (MQ=11)

                                                                                                                          aaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATg                 <  1:184506/141‑1 (MQ=255)

                                                                                                                          aaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATg                 >  1:86011/1‑141 (MQ=255)

                                                                                                                          aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCCATACCTTTTCAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                 <  2:306706/141‑1 (MQ=255)

                                                                                                                          aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATTCCTTGTCTTTCTTACTTTAATATg                 >  1:244027/1‑141 (MQ=25)

                                                                                                                          aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatata                 >  2:131051/1‑140 (MQ=21)

                                                                                                                          aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                 >  1:330339/1‑141 (MQ=21)

                                                                                                                          aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                 <  2:172646/141‑1 (MQ=25)

                                                                                                                          aaTTTCGTGGCATGGCGAAAAGCCGTAGATCGGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                 <  2:24452/141‑1 (MQ=255)

                                                                                                                           aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGa                >  2:313920/1‑141 (MQ=11)

                                                                                                                            ttgccggcgaTGGCGAAAATCCGGAGATCTGAAGAGACCTGCGGTTCTGTTTATATAGGGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATGCCTTGTCTTTCTTACTTTAATATGAc               <  2:237357/132‑1 (MQ=11)

                                                                                                                             ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg              >  1:334584/1‑141 (MQ=25)

                                                                                                                              tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg             >  2:14304/1‑141 (MQ=37)

                                                                                                                               cctGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAAAGAGCGTACATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt            <  1:165889/139‑1 (MQ=255)

                                                                                                                                gTGGCTTGGCGAAAGTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg           <  2:107997/141‑1 (MQ=255)

                                                                                                                                gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTTTATCGGGCGTAAATACCCTCAAGCACTCTGAATATTTTCTTTGCTGTATTGCTACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg           <  2:166/141‑1 (MQ=11)

                                                                                                                                gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATAACGGTg           >  1:162956/1‑141 (MQ=37)

                                                                                                                                gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATCCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg           <  1:254957/141‑1 (MQ=255)

                                                                                                                                 tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa          >  1:381790/1‑141 (MQ=38)

                                                                                                                                 tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTACTCACTTTAATATGACGgaga          >  1:381863/1‑141 (MQ=255)

                                                                                                                                 tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTAGTCTTTCTTACTTTAATATGACGGTGa          >  2:7485/1‑141 (MQ=255)

                                                                                                                                  ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACAGTAAt         >  1:196428/1‑141 (MQ=12)

                                                                                                                                   gCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATc        >  2:53574/1‑141 (MQ=11)

                                                                                                                                     aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt      >  2:218936/1‑141 (MQ=255)

                                                                                                                                     aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTATTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt      <  1:259048/141‑1 (MQ=255)

                                                                                                                                      tGGCGAACATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg     <  1:268533/141‑1 (MQ=255)

                                                                                                                                      tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg     <  1:604/141‑1 (MQ=255)

                                                                                                                                       ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGGTCTTGc    >  1:6593/1‑141 (MQ=255)

GAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTC  >  CP000730/35776‑36053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`