Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,930	T→A	55.0%	intergenic (‑1013/‑155)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,930	0	T	A	55.0%	9.1 / 130.0	91	intergenic (‑1013/‑155)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (17/23);  new base A (31/17);  total (51/40)
Fisher's exact test for biased strand distribution p-value = 5.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-02

TATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATT  >  CP000730/1929790‑1930068                                                                                                                                                  |                                                                                                                                           tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTGTGCCATTGCGttttttt                                                                                                                                            <  1:232460/141‑1 (MQ=255)     ttcacagagggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑A‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                       >  2:264304/12‑141 (MQ=255)       tGCCGATGATATAATAGTAGAGTCTCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAtt                                                                                                                                      <  1:336731/141‑1 (MQ=255)       cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                     >  1:94314/5‑141 (MQ=255)        acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                                                                                    <  2:293779/138‑1 (MQ=255)         cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                                   >  2:47341/3‑141 (MQ=255)         cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                                   >  2:265441/3‑141 (MQ=255)         cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                                   >  1:284786/3‑141 (MQ=255)          agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                                                                                  >  1:288208/2‑141 (MQ=255)          agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                                                                                  >  2:57144/2‑141 (MQ=255)           gatggtATAGTAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGGG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                                                                                 <  2:228541/141‑2 (MQ=255)             tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCCT‑TCC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                               >  1:53099/4‑138 (MQ=255)             tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                                                                               >  1:378026/4‑141 (MQ=255)             tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTaa                                                                                                                                >  2:102514/1‑141 (MQ=255)             tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTaa                                                                                                                                >  2:362424/1‑141 (MQ=255)             gggtataATAATAGAGCCGCCTATCTCTCAGGCGCCAATT‑‑GACGAAGAGAGGAGGTGCGT‑TAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGCTGCGTATTATGCTTATTGGCTTAg                                                                                                                               >  1:230187/4‑141 (MQ=255)              ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCACTT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                              >  1:264537/3‑141 (MQ=255)              ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGTGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                              <  1:374042/139‑1 (MQ=255)              ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCCGTGGCTGTGCTGTTGCGTATTATCCTTCTTGGCTTAGt                                                                                                                              >  2:56730/3‑141 (MQ=255)              ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                              >  2:236422/3‑141 (MQ=255)              ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                              >  1:301275/3‑141 (MQ=255)              ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                              <  2:190065/139‑1 (MQ=255)              ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATAATAGCACCAGTCATCGGTGGCTGTGATGTTGCGTATTATCCTTATTGGCttggt                                                                                                                              >  2:90679/3‑137 (MQ=255)              gatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                                                                                               <  2:310633/141‑1 (MQ=255)               gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                             >  2:115820/2‑141 (MQ=255)               gtataATAATAGAGACGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                             >  1:200061/2‑141 (MQ=255)                tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                            >  2:291491/1‑140 (MQ=255)                tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                            >  2:320387/1‑140 (MQ=255)                tataATAATAGAGTCGCCTATCTCTCAGGAGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                            <  1:258578/141‑2 (MQ=255)                  taataaTAGAGTCGCCTATGTCTCAGGCGTCAATT‑‑GACGAAGATAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc                                                                                                                          >  1:314965/1‑141 (MQ=255)                  taataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGa                                                                                                                           <  1:252728/141‑1 (MQ=255)                   aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                                                                                         >  1:118489/1‑141 (MQ=255)                   aataatAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGATGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                                                                                         >  1:30782/1‑141 (MQ=255)                    atatTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGGGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCTGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttactaaacgc                                                                                                                        <  2:269373/141‑11 (MQ=255)                    ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                        <  2:66599/137‑1 (MQ=255)                    agaagagagCCGCCTATCGCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑G‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                        <  1:267206/136‑1 (MQ=255)                     taatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                                                                                       <  2:282803/138‑1 (MQ=255)                     taatagAGTCGCCTATCTCTCAGGCGTCAAAT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                                                                                       <  1:127303/138‑1 (MQ=255)                       atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGGGTATTGTACTTATTGGCTTGGTAAACGCaac                                                                                                                     >  1:20020/2‑140 (MQ=255)                        tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                                                                                                    <  2:15153/141‑3 (MQ=255)                         agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                                                                                   >  2:64214/1‑138 (MQ=255)                          gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                                  <  1:289772/141‑5 (MQ=255)                          gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                                  >  1:75619/1‑137 (MQ=255)                          gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGAAAACGCaacaaa                                                                                                                  >  2:214494/1‑137 (MQ=255)                           agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGGAAACGCaacaaat                                                                                                                 >  2:353305/1‑136 (MQ=255)                           agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                                 <  1:52210/141‑6 (MQ=255)                            gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                                                                                >  2:143415/1‑135 (MQ=255)                             tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataa                                                                                                               >  2:215102/1‑134 (MQ=255)                              cGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaaaa                                                                                                               >  1:153740/1‑141 (MQ=255)                              cGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaaaa                                                                                                               <  1:103237/141‑1 (MQ=255)                               gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                                                                                                              >  1:182836/1‑141 (MQ=255)                               gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                                                                                                              <  1:258115/141‑1 (MQ=255)                               gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                             <  2:10466/141‑10 (MQ=255)                               gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                             >  2:359251/1‑132 (MQ=255)                               gCCCATCTCTCAGGCGTCAATT‑‑GGCGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                             <  2:354581/141‑10 (MQ=255)                                 cTATCTCTCCAGCGTCAATTTAGAGGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGCTGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAg                                                                                                            >  1:280001/1‑141 (MQ=255)                                 cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                                           >  1:223191/1‑130 (MQ=255)                                 cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                                           <  1:241598/141‑12 (MQ=255)                                  tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                          >  1:35512/1‑129 (MQ=255)                                   aTCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGt                                                                                                          <  2:382392/141‑1 (MQ=255)                                   aTCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGAGGCTTATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTCGACGCAAGACAAACTactt                                                                                                          >  1:80999/1‑138 (MQ=255)                                   aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                                         <  1:362206/141‑14 (MQ=255)                                   aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                                                                         >  1:144825/1‑128 (MQ=255)                                    tctctcAGGCGTCAATT‑‑GACGAAGGGAGGAGGTGCGT‑TAC‑T‑T‑TGCTACTCATTTTCGGTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATAGTTATTGCCTTAGTAAACGCaaccagtatttaattg                                                                                                        >  1:124039/1‑127 (MQ=255)                                        ccAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTCATGGATGAGTATACGCAATACAAAATAGGTGACat                                                                                                     <  1:185038/140‑1 (MQ=255)                                        ccAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACat                                                                                                     <  2:363207/140‑1 (MQ=255)                                         cAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGTAATACAAAATAGGTGACata                                                                                                    >  1:282712/1‑141 (MQ=255)                                         cAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata                                                                                                    <  2:321808/141‑1 (MQ=255)                                                 aaTTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa                                                                                            <  1:277385/141‑1 (MQ=255)                                                 aaTTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCa                                                                                            <  1:218594/141‑1 (MQ=255)                                                     tAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCaa                                                                                        <  1:68106/141‑1 (MQ=255)                                                          gCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGATGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaa                                                                                   <  1:141597/141‑1 (MQ=255)                                                            agagagGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAAt                                                                                 >  1:146014/1‑141 (MQ=255)                                                                aggaggTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATcccc                                                                             >  1:82811/1‑141 (MQ=255)                                                                    ggTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCAc                                                                         <  1:362424/141‑1 (MQ=255)                                                                    ggTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCAc                                                                         <  1:102514/141‑1 (MQ=255)                                                                         ta‑taA‑G‑G‑TGGTGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGACGCACCAATAACAATCTCCTCACTAcg                                                                     >  2:334798/1‑140 (MQ=255)                                                                                        gCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGa                                                          >  1:98768/1‑141 (MQ=255)                                                                                        gCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGa                                                          >  2:374656/1‑141 (MQ=255)                                                                                          ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                                        <  2:280001/141‑1 (MQ=255)                                                                                           tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTACGTAGAAGCAATAAAAAGTAGGTGACATATAGCCGCACCAATGAAAAACCCCACACTACCGAAAATAgaaggcg                                                       >  2:199963/1‑135 (MQ=255)                                                                                           tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAggg                                                       <  2:333771/141‑1 (MQ=255)                                                                                             ttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGa                                                     <  2:136971/141‑1 (MQ=255)                                                                                                  gTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTggg                                                >  1:177755/1‑140 (MQ=255)                                                                                                  gTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGgt                                                >  1:349389/1‑141 (MQ=255)                                                                                                   ttCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATACCCTCACTACCGCAGATAGCGAGGGAATTGgtg                                               >  1:351239/1‑141 (MQ=255)                                                                                                    tCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGgtgt                                              <  2:166975/141‑1 (MQ=255)                                                                                                             ggcatccgTCATCAGTGGCTGTTCTATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTaaa                                     <  2:70321/134‑1 (MQ=255)                                                                                                                 ccAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACt                                 <  1:40548/141‑1 (MQ=255)                                                                                                                    gTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAt                              <  2:153740/141‑1 (MQ=255)                                                                                                                               gtgtgCCTTTGCGTTTTTTTCTTATTGGCGAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCt                   <  2:324162/140‑1 (MQ=255)                                                                                                                                         gCGTTTTTTTTTTATTGTCTAAGTAGACGCAATACAAAATATTTGACATGTAGCCGCGCCAATCGAACTGCCCTCACTACCGCAATACGTGCGGGGCTTGGTGTATATGTATATATTTATTTTCGTACTCTAAATtagagt         >  2:222828/1‑137 (MQ=255)                                                                                                                                                tttCTTATTGGCTAAGTAGACGAAATACAAAATAGGTGACATATAGGCGCACCAATAAAAATCCCCTCACTACCGCAAAGAGTGAGGGGGTTTGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAAtt  <  2:80999/141‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                           TATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATT  >  CP000730/1929790‑1930068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑