Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8120GA67.4% 359.9 / 310.5 355intergenic (‑895/‑273)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (50/65);  new base A (105/133);  total (155/200)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.98e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 387 total aligned reads displayed.

TCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAA  >  CP000730/1929672‑1929952
                                                                                                                                            |                                                                                                                                                 
 cGCCTACTTTCTTAAATTAACAGTATCTATTGTCATAGAATTTGTCCAATTAAGAGTTGATGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGGCTGTGGTTGTATATTTGGCGATGATATAAtagt                                                                                                                                                  >  1:1132925/1‑141 (MQ=37)
    cTAGTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGTAAGTGACTGTGCTTGTATATTTGTCGATGATTTAAtattata                                                                                                                                               <  2:405779/141‑3 (MQ=25)
     tACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTagag                                                                                                                                              <  2:207867/141‑1 (MQ=255)
                aTTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAtctc                                                                                                                                   <  1:155161/141‑1 (MQ=255)
                 ttAACAATATCTATTCGCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGCGTCATATAACTTATCAGAAGCAGCTGCCTGTGCTAGTATGTTTGCCGATGACATAATAGTATGGTCGCCCAtatcc                                                                                                                                  >  2:421426/1‑140 (MQ=21)
                    aCAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAg                                                                                                                               <  1:132299/141‑1 (MQ=255)
                    aCAATATCTATTCTCATAGAATTTGTCCAATTAAGGGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAg                                                                                                                               <  1:349612/141‑1 (MQ=255)
                       atatCTATTCTCATAGAATTTGTCCAATTAAGTGTAGCCGCTTCAATCGGTCATCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCg                                                                                                                            <  1:686232/141‑1 (MQ=39)
                       atatCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCg                                                                                                                            <  2:1169565/141‑1 (MQ=255)
                           cTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCaa                                                                                                                        <  1:393840/141‑1 (MQ=255)
                            tATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAAt                                                                                                                       >  1:1068243/1‑141 (MQ=255)
                               tctcATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTa                                                                                                                    >  2:921466/1‑141 (MQ=255)
                                   aTAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACg                                                                                                                <  2:979396/141‑1 (MQ=255)
                                       aaTTTTTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCaga                                                                                                            <  2:876665/141‑1 (MQ=255)
                                       aaTTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCaga                                                                                                            <  1:721872/141‑1 (MQ=255)
                                        aTTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagag                                                                                                           <  2:1068243/141‑1 (MQ=255)
                                         tttGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCagaga                                                                                                          >  1:502613/1‑141 (MQ=255)
                                                             cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGGG‑TA‑TAA‑G‑G‑TGATGc                                                                                 >  1:895352/1‑141 (MQ=255)
                                                              gATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCt                                                                                <  2:230390/141‑1 (MQ=255)
                                                                 tcagtcttgcAGCGAGCATCATATCACGTATGCGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTAt                                                                             <  2:1112274/132‑1 (MQ=255)
                                                                    aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGGGGTG‑TA‑TAA‑G‑G‑TGATGCTTAtttt                                                                          >  1:366859/1‑141 (MQ=255)
                                                                     agtatgagggaGAATGATATAACTTATCAGAGGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTc                                                                         <  2:1186109/131‑1 (MQ=255)
                                                                                    cATTTAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCa                                                          <  2:667652/141‑1 (MQ=255)
                                                                                    cATATATCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCa                                                          <  2:758778/141‑1 (MQ=255)
                                                                                     atatAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCAc                                                         >  2:905817/1‑141 (MQ=255)
                                                                                         aaCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGt                                                     >  1:825230/1‑141 (MQ=255)
                                                                                          aCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGtc                                                    >  1:962606/1‑141 (MQ=255)
                                                                                              aTCAGAAGCAAGTGACTTTGCGTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑AA‑TCA‑T‑G‑CGATGCTTATTTTCGTTCACATCATAGGACCAGtcatca                                                >  2:543634/1‑141 (MQ=255)
                                                                                                  atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                           >  2:1130863/15‑141 (MQ=255)
                                                                                                   tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                          >  1:262701/14‑141 (MQ=255)
                                                                                                   tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTGGTTCACATCATAGCCCCCGGCACCAGTGGcg                                          >  1:121243/14‑140 (MQ=255)
                                                                                                    cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                         >  1:308798/13‑141 (MQ=255)
                                                                                                    aggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                         <  1:321073/129‑1 (MQ=255)
                                                                                                     ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGGTGTG‑CA‑TGACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                        <  1:334392/130‑1 (MQ=255)
                                                                                                     ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                        <  1:910644/130‑1 (MQ=255)
                                                                                                      gaagtagttatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATATCACCTGTCATCAGTGGCtgtg                                       <  1:617421/131‑1 (MQ=255)
                                                                                                      gaagtagatattcTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCTTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                       <  2:1100708/129‑1 (MQ=255)
                                                                                                      gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                       <  1:1178052/131‑1 (MQ=255)
                                                                                                      gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                       <  1:474479/131‑1 (MQ=255)
                                                                                                      gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                       <  1:513184/131‑1 (MQ=255)
                                                                                                      gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGCCATAAAGT‑‑GGCGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                       <  1:877687/131‑1 (MQ=255)
                                                                                                      gaagtagaaatgCTTGTATATTCACAGAGGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                       <  1:447535/131‑1 (MQ=255)
                                                                                                       aagtagatatgtttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                      <  2:950398/129‑1 (MQ=255)
                                                                                                       aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                      <  1:1106143/132‑1 (MQ=255)
                                                                                                       aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                      <  2:330078/132‑1 (MQ=255)
                                                                                                       aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                      <  1:339545/132‑1 (MQ=255)
                                                                                                        agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                     <  1:677263/133‑2 (MQ=255)
                                                                                                        agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                     <  1:594340/133‑2 (MQ=255)
                                                                                                        aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                      <  1:1146176/141‑1 (MQ=255)
                                                                                                         gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTgcgcgg                                    <  2:142043/134‑3 (MQ=255)
                                                                                                         gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctt                                    >  2:246288/8‑139 (MQ=255)
                                                                                                         gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                    >  2:1041464/8‑139 (MQ=255)
                                                                                                          tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                   <  2:561478/135‑4 (MQ=255)
                                                                                                           agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                  <  1:941307/136‑5 (MQ=255)
                                                                                                           agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                  >  2:150655/6‑137 (MQ=255)
                                                                                                            gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAATGGCTGGGctgttg                                 >  2:1089659/5‑136 (MQ=255)
                                                                                                            aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                  <  1:1077350/141‑1 (MQ=255)
                                                                                                            aCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                  <  1:651911/141‑1 (MQ=255)
                                                                                                             cTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGTTGTGCCATTg                                 <  1:792670/141‑1 (MQ=255)
                                                                                                               gtgCTTGTATATTTGCCGATGATATTATCTTAGAGGTGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTC‑TA‑TAA‑G‑G‑TGATGCTTATTTGCGTTCACATCATAGCTCCAGTCATCAGTGGCTGTGCCATTGCg                               <  2:806969/141‑1 (MQ=255)
                                                                                                               gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg                               >  2:132190/1‑141 (MQ=255)
                                                                                                               atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                              <  2:1002940/140‑1 (MQ=255)
                                                                                                               atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGGGCTGTTGCGt                              >  1:700241/2‑141 (MQ=255)
                                                                                                                 gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                            <  2:363169/141‑3 (MQ=255)
                                                                                                                  cTTGTATATTCACAGATGGTATAGTAATAGGGTCGCCGATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGGGGGG‑CATTAC‑T‑T‑TGCTAATCGTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                           <  1:101703/141‑1 (MQ=255)
                                                                                                                   ttGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttt                           <  1:988902/141‑1 (MQ=255)
                                                                                                                    tGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                          >  2:515219/1‑141 (MQ=255)
                                                                                                                     gTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttttt                         <  2:831638/141‑1 (MQ=255)
                                                                                                                     gTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttttt                         <  2:638786/141‑1 (MQ=255)
                                                                                                                      tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttttt                        <  2:180408/141‑1 (MQ=255)
                                                                                                                      tataTTCACGGATGGTATAATAATAGAGTCGCCTATCTCTCCGGCGTCAATT‑‑GACTAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                       <  2:301089/141‑5 (MQ=255)
                                                                                                                      tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAAAT‑‑GAATTGGTGTGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                       <  1:275746/141‑8 (MQ=255)
                                                                                                                       atatTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTGCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTc                       <  2:349701/141‑1 (MQ=255)
                                                                                                                        tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTGTTATACtt                     >  1:1041096/9‑141 (MQ=255)
                                                                                                                          ttcccagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                   <  2:599082/135‑1 (MQ=255)
                                                                                                                           ttGCCGATGATATAATAGTAGAGGCGCCTATCTCACAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAt                   <  2:124380/141‑1 (MQ=255)
                                                                                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                 >  2:470278/5‑141 (MQ=255)
                                                                                                                             acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                <  1:1000429/138‑1 (MQ=255)
                                                                                                                              cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTCTACTTATTGGc               >  1:748148/3‑141 (MQ=255)
                                                                                                                              cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc               >  1:193535/3‑141 (MQ=255)
                                                                                                                               agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGNGTATTATACTTATTGGCt              >  1:1096287/2‑141 (MQ=255)
                                                                                                                               agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt              >  2:615454/2‑141 (MQ=255)
                                                                                                                               agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt              >  1:174487/2‑141 (MQ=255)
                                                                                                                                gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGACAAGGAG‑TA‑TTA‑GTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt             >  1:227178/1‑140 (MQ=255)
                                                                                                                                  tggtataATAATAGAGTCGCCTATCTGTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag           <  2:843251/138‑4 (MQ=255)
                                                                                                                                  tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag           <  2:963276/138‑4 (MQ=255)
                                                                                                                                  tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg           >  1:556320/4‑141 (MQ=255)
                                                                                                                                  tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag           <  2:576115/138‑4 (MQ=255)
                                                                                                                                   ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GCGGAAGAGAGGAGGTGCAA‑TAC‑T‑T‑TGCTAATGATTTTCGTTCACATCATAGCACGAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAgg          >  1:422591/3‑140 (MQ=255)
                                                                                                                                   ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt          >  1:1165538/3‑141 (MQ=255)
                                                                                                                                    gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTCCTGTTCGGTATTATACTTATTGGCttggta         >  2:114599/2‑136 (MQ=255)
                                                                                                                                    atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt          >  1:416858/1‑141 (MQ=255)
                                                                                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa       >  2:1020498/1‑139 (MQ=255)
                                                                                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa       <  1:204299/141‑3 (MQ=255)
                                                                                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa       <  1:743065/141‑3 (MQ=255)
                                                                                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa       <  1:65687/141‑3 (MQ=255)
                                                                                                                                      acaataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa       >  2:324546/3‑139 (MQ=255)
                                                                                                                                       taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac      <  2:822873/141‑4 (MQ=255)
                                                                                                                                       taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc      >  2:708031/1‑141 (MQ=255)
                                                                                                                                       taataaGAGAGTCCTCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc      <  2:273585/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
TCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAA  >  CP000730/1929672‑1929952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑