Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,360,799 A→G 100% G66G (GGT→GGC adk ← adenylate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,360,7990AG100.0% 99.1 / ‑6.5 37G66G (GGT→GGCadkadenylate kinase
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (16/18);  total (19/18)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGATGACAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATACCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTCTTGA  >  CP000730/2360663‑2360935
                                                                                                                                        |                                                                                                                                        
tgatgaCAGCATTAATGTTTCTGTCAAGCTCAGAGATTATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCt                                                                                                                                       <  2:275788/140‑1 (MQ=255)
 gatgaCAGCATCAATGTTTACCTGCACCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTa                                                                                                                                      >  1:354306/1‑140 (MQ=255)
        gCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCaa                                                                                                                               >  1:294733/1‑139 (MQ=255)
          aTCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCGACAGTCACtt                                                                                                                             <  2:159111/140‑1 (MQ=255)
           tCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTtc                                                                                                                            <  1:287847/140‑1 (MQ=255)
              aTGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCCCTtcatc                                                                                                                         >  2:337762/1‑140 (MQ=255)
                    cTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATGTAATAAAAAGCCTTTTTTTGGATGGTCTTCAGAACTTCTTTCTTTAACGATTCTTACAGTCACTTAATGAgggca                                                                                                                   >  2:83327/1‑137 (MQ=255)
                          atcTCAGACATAACATTATTTAATGCCTCAGCTTGCTCGATTGTTCTGGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTc                                                                                                             <  1:333108/138‑1 (MQ=255)
                          aGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAACTTCTTCACTTAACAAGACCTACAGTCACTTCATCAGGAACTAATTc                                                                                                             >  1:274745/1‑140 (MQ=255)
                              cAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGccc                                                                                                         <  2:255090/140‑2 (MQ=255)
                              cAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATAAGGAACTAATTCGCCa                                                                                                         <  1:176578/140‑1 (MQ=255)
                                  cATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTAATCAGGAACTAATTCGCCACGGt                                                                                                     >  1:20397/1‑140 (MQ=255)
                                      atatTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCTTACAGTCACTTCATCAGGAACTAATTAGCAAAGGTCCat                                                                                                 >  1:109533/1‑140 (MQ=255)
                                              tAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACt                                                                                         <  1:277768/140‑1 (MQ=255)
                                               aaTGCCGCAGCTTGCATGGTTGGTCTTGGGAAGCCATCGAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATGCTTTCCGTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACtt                                                                                        <  1:312849/140‑1 (MQ=255)
                                                     tCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTGGACACGGTCCATATAAGAATTAGctta                                                                                  >  2:103655/1‑139 (MQ=255)
                                                     tCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCCCGGCCCAAATAAGACTTAGcttc                                                                                  >  1:92979/1‑140 (MQ=255)
                                                           tGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTAcc                                                                            <  1:42125/140‑1 (MQ=255)
                                                           tGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCGTCAGTAATTCTTTCCTTAACGATGCCTACACTCCCTTCATCAGGAACTAATTCGCCACGGTCCATATAATACTAAGCTTCTTTAcc                                                                            >  2:37711/1‑140 (MQ=255)
                                                            gCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGAAATTAACAAACACGTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTCCCt                                                                           >  1:350686/1‑140 (MQ=255)
                                                                 aTTGTTCTTGGGAAGCCATCTGATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTc                                                                      <  1:71921/140‑1 (MQ=255)
                                                                  ttGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCa                                                                     >  2:269244/1‑140 (MQ=255)
                                                                      tCTTGGGAAGCCATCTAATAAAGATCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTAAAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGttt                                                                 <  1:95951/140‑1 (MQ=255)
                                                                       cTTGGGAAGCCATCTAATAATAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTAAGTttc                                                                >  1:225298/1‑140 (MQ=255)
                                                                       cTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTttc                                                                >  1:251570/1‑140 (MQ=255)
                                                                              aGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTTAGTTTCTTCtttt                                                         >  1:7760/1‑140 (MQ=255)
                                                                               gCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTta                                                        <  2:319116/140‑1 (MQ=255)
                                                                                            aaGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGaa                                           >  2:59948/1‑140 (MQ=255)
                                                                                                  ttttttGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTAATAGCTTTTCTGCACATgcc                                     >  1:106451/1‑138 (MQ=255)
                                                                                                        gCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCCGt                               >  1:357109/1‑140 (MQ=255)
                                                                                                          aTCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATTCATATGCTGAACATGTCACCAGTTg                             >  2:249016/1‑140 (MQ=255)
                                                                                                                 tCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTCATTCGCCCCGGTCCATATAAGACTTCGCTTCTTTACCTATTTCGGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAAtgtg                      <  1:70800/140‑1 (MQ=255)
                                                                                                                 tCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAAtgtg                      <  2:320218/140‑1 (MQ=255)
                                                                                                                 tCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATCCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCCCCAGTTGAAAtgtc                      <  2:314110/140‑2 (MQ=255)
                                                                                                                             gtcTTATCGAGGCCGACAGTCACTTCATCTGGACCTAATTTGCCACGGTCCATATAACACTTAGCTTCTTTACCTCATTCCGTTTCTTCTTTAATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGaa          <  2:87639/138‑1 (MQ=255)
                                                                                                                             gctttAACGATGCCTACAGTCTCTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATCGCTCTAGTTGAGATGTGGGGTATTGGGaa          <  2:148083/137‑1 (MQ=255)
                                                                                                                                     gATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTCTTGa  <  1:341207/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TGATGACAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATACCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTCTTGA  >  CP000730/2360663‑2360935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: