Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,532,298 A→T 100% T15T (ACA→ACT)  murN → FemAB family peptidoglycan biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,532,2980AT100.0% 149.7 / NA 52T15T (ACA→ACT) murNFemAB family peptidoglycan biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (16/36);  total (16/36)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA  >  CP000730/2532165‑2532433
                                                                                                                                     |                                                                                                                                       
tATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                   <  1:155964/140‑1 (MQ=255)
tATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                   <  1:173370/140‑1 (MQ=255)
 tgatGTAAAAAGATTTTGATAAAATAAAAAAATAGGATTATGAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc                                                                                                                                  <  1:87188/137‑1 (MQ=255)
       aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGACGGATGGGATAACATGCCCTTTGTAACGTTCACTTCGTTTGAGTTCTATGCCTTTACTACagagtgggttt                                                                                                                            <  2:32280/140‑8 (MQ=255)
         tCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATAAAAGTTCAATTCAGAGAGGGATAGGAAAAAAAGAACTATATAACGATGACTACGGAAGAGTTCAATGCGATTACTACAAAGCATTTTTca                                                                                                                          >  1:2680/1‑140 (MQ=255)
          cAGTTTTTGATAAAAAAAAAAAATCGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTcac                                                                                                                         <  2:18611/140‑1 (MQ=255)
           aGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATGCAGAGGGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTcaca                                                                                                                        >  1:74944/1‑140 (MQ=255)
                      aaataaaaAAATAGCATTATTGATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTGGGAACGTTGACTTCAGATGAGTTCAATGCGGTTACTACAAAGCATTTTTCACAATAcacacaca                                                                                                             >  2:244243/1‑138 (MQ=255)
                          aaaaaaaTAGCATTATTAATAAATTCAAAAACTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc                                                                                                         <  1:16370/140‑1 (MQ=255)
                             aaaaTAGCATTATTAATAAAGTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTAACATCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACAAACAATCAgatct                                                                                                      >  1:189423/1‑136 (MQ=255)
                               aaTAGTATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGACAAAATGAACTGTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTc                                                                                                    <  1:26806/140‑1 (MQ=255)
                               aaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTc                                                                                                    >  1:335805/1‑140 (MQ=255)
                                aTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACATACAATCAGCTATTCa                                                                                                   <  1:178948/140‑1 (MQ=255)
                                aTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCa                                                                                                   <  1:205299/140‑1 (MQ=255)
                                 tAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCAt                                                                                                  <  1:68708/140‑1 (MQ=255)
                                       tattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAt                                                                                            >  1:273594/1‑140 (MQ=255)
                                        gttggtaaGTTCATAAAGTATAGTTGAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATc                                                                                           <  2:90058/135‑1 (MQ=255)
                                           aataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATa                                                                                        <  1:54488/140‑1 (MQ=255)
                                            ataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATag                                                                                       <  2:9587/140‑1 (MQ=255)
                                            ataaATTCAAAAATCAAAATTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATag                                                                                       <  1:303598/140‑1 (MQ=255)
                                               aaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGt                                                                                    <  1:2142/140‑1 (MQ=255)
                                                      ataTTGTAATTGAATTAAGACAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACTCACAATTAGCTATTCATTACAATCATAGAGTTGATTTa                                                                             <  2:307323/138‑1 (MQ=255)
                                                             aGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg                                                                      <  1:139891/140‑1 (MQ=255)
                                                             aGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg                                                                      <  2:356237/140‑1 (MQ=255)
                                                                 cAATTCAGAGAGGGATGGGATAAAATTAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACCCAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtgt                                                                  <  2:59026/140‑1 (MQ=255)
                                                                 cAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtgt                                                                  <  2:33796/140‑1 (MQ=255)
                                                                  aaTTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtgtg                                                                 <  2:271106/140‑1 (MQ=255)
                                                                     tCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGCCGATGTGAAt                                                              >  2:210238/1‑140 (MQ=255)
                                                                     tCAGAGAGGGATGGGATAAAATGAACTTTGTAAAGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATAAGCTATTCATTACAATCATATAGTTGATTTAACAGACGATGTGCAt                                                              >  2:210261/1‑140 (MQ=255)
                                                                       agagggGGGTGTGATAAAATGAACTTTTTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt                                                            <  2:102953/140‑1 (MQ=255)
                                                                         ggagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTCCTCCAAAGCAAGTAGCACAGTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTg                                                          <  1:14022/139‑1 (MQ=255)
                                                                                tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTCTTCATAACAATCCTTGCGTTGATTTAAAAGGAGATGTGCATCTTGTAGGGGt                                                   >  1:81208/1‑140 (MQ=255)
                                                                                tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                   <  1:333086/140‑1 (MQ=255)
                                                                                   gatAAAATGAACTGTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTaa                                                <  1:73552/140‑1 (MQ=255)
                                                                                         aTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGa                                          >  2:289858/1‑140 (MQ=255)
                                                                                            aaCTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACaa                                       <  2:338034/140‑1 (MQ=255)
                                                                                             aCTTTGTAACGTTGACTTCGGATGAGTTAAATGCGTTTAATACAAAGCATTTTTCACATTACACACAATGAGCTATTCATTACAATCATAGAGTTGGTTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAACATGACAAt                                      >  2:50635/1‑140 (MQ=255)
                                                                                                ttGAAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGCGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGCTAAAGATGCCCATgat                                   >  1:256353/1‑138 (MQ=255)
                                                                                                      tgTTGACTTCGGATGAGTTCTATGCGTTTACTTCAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTg                             <  1:296251/139‑1 (MQ=255)
                                                                                                           aCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTTTTCATTACAATCATAGAGTTGATTTAAAAGGCGTTGTGCATGTTGTAGGGTTTATGGTTGATTTTGGTCTAGTGTTTGc                        >  1:35146/1‑140 (MQ=255)
                                                                                                           aCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGTTGACAATGGTCATGTGTTTGc                        >  1:35116/1‑140 (MQ=255)
                                                                                                           aCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGc                        <  1:53460/140‑1 (MQ=255)
                                                                                                            cTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCa                       >  1:214998/1‑140 (MQ=255)
                                                                                                             gtCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAg                      <  2:107125/139‑1 (MQ=255)
                                                                                                               cGGATTAATTCAATGCGTTTACTACTAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTACAAGGCGATGTGCATCTTGTAGGGGTTAAACATGACAATGGTCAAGTGATTGCAGGa                    <  2:221345/140‑1 (MQ=255)
                                                                                                                ggctGAGTTCAAGGCGTTTACTACAAAGCATTTTTCACATTACACAATTGCCGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                   <  2:250365/137‑1 (MQ=255)
                                                                                                                      gTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAt             >  2:210167/1‑140 (MQ=255)
                                                                                                                       ttCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAtt            <  1:93426/140‑1 (MQ=255)
                                                                                                                            tGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAg       <  2:353624/140‑1 (MQ=255)
                                                                                                                             gCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGa      <  1:11035/140‑1 (MQ=255)
                                                                                                                                 ttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgca  <  1:254230/140‑1 (MQ=255)
                                                                                                                                 ttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgca  <  1:17894/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
TATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA  >  CP000730/2532165‑2532433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑