| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,709,297 | T→A | 28.0% | K544* (AAA→TAA) | oatA ← | possible acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,709,297 | 0 | T | A | 28.0% | 36.6 / 16.1 | 25 | K544* (AAA→TAA) | oatA | possible acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (6/12); new base A (2/5); total (8/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
GCTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAA > CP000730/2709170‑2709416 | gcTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTAcc < 2:328857/140‑1 (MQ=255) tttACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCtt < 2:71468/140‑1 (MQ=255) ctacCTGCATATTATAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGATTTTCAGCTGACCCACCAGTTAATGTATTTATTCTACCTTCATa > 2:73819/1‑140 (MQ=255) tacCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATaa > 2:240550/1‑140 (MQ=255) tGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCACAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCACAAtctc < 2:196380/140‑1 (MQ=255) tCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTAc < 1:37346/140‑1 (MQ=255) tAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTActc < 1:200119/140‑1 (MQ=255) tAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTActc < 1:254030/140‑1 (MQ=255) gAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATCATCTCTAGGTACTCTAATa < 1:213580/140‑1 (MQ=255) tGCAAAGTATTCCGGATGACCTGCAGAAGCATTATACCAATCGACTGGATGTACGTTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAatata > 2:162535/1‑140 (MQ=255) aaGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTc < 2:321078/140‑1 (MQ=255) aaGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTc < 2:97326/140‑1 (MQ=255) aaGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTc < 2:240710/140‑1 (MQ=255) gTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTg < 2:279920/140‑1 (MQ=255) aTTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCt < 1:314681/140‑1 (MQ=255) ttCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCtt < 2:22003/140‑1 (MQ=255) aCCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAAc < 2:196108/140‑1 (MQ=255) cAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATcc > 2:52819/1‑140 (MQ=255) taGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAAt < 1:103883/139‑1 (MQ=255) cgttATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATTGt < 2:5183/138‑1 (MQ=255) cTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATATATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGt < 2:195523/140‑1 (MQ=255) taCCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACGCTAATAGATACTAAATATATGTCTGCTTTTCTAAAACTATCCACTAGTTCAtt > 2:188335/1‑140 (MQ=255) cATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAAt > 1:185439/1‑140 (MQ=255) gcgcTTTTCAGCTGCCTCATGAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTAAATTGATCTTTCGTAAATGCCCCAt > 1:215846/1‑140 (MQ=255) aGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAATTCATTTAATTGATCTTTCGTAAATCCCCCATTTGTACCaa > 1:23757/1‑140 (MQ=255) | GCTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAA > CP000730/2709170‑2709416 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |