New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	= 1205323	51 (0.630)	32 (0.550) +AGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAG	8/200	8.2	46.4%	intergenic (+459/‑365)	yfiE/lspA	possible lactoylglutathione lyase/A8 family signal peptidase II
	?	CP000730	= 1443739	NA (NA)					intergenic (‑312/+41)	USA300HOU_1341/brnQ2	IS1272 transposase/LIVCS family branched chain amino acid:cation symporter
Rejected: Coverage evenness skew score above cutoff.

AGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAACCACTCTAAAGAA  >  CP000730/1443621‑1443879                                                                                                                          |                                                                                                                                             aGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCaa                                                                                                                        <  2:547560/141‑1 (MQ=11)  gTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAAc                                                                                                                       >  1:689666/1‑141 (MQ=31)   tCGAAGACCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAAcc                                                                                                                      <  1:167818/141‑1 (MQ=32)        gACCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCttt                                                                                                                 <  2:838190/141‑1 (MQ=35)         aCCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCtttt                                                                                                                <  1:114642/141‑1 (MQ=35)           cGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTtat                                                                                                              <  1:691754/141‑1 (MQ=35)            gAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTtata                                                                                                             >  1:114285/1‑141 (MQ=35)              ggCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTAtatt                                                                                                           >  2:117092/1‑141 (MQ=37)               gCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTTGTTATTTATGTCCCAACCTCTTTTAtatta                                                                                                          >  2:394177/1‑141 (MQ=255)                  gagaCGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTc                                                                                                       <  2:767678/141‑1 (MQ=39)                    gaCGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGt                                                                                                     <  2:860543/141‑1 (MQ=255)                    gaCGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGt                                                                                                     <  1:667941/141‑1 (MQ=255)                     aCGGCAGCCTAGGAGAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGtt                                                                                                    <  2:449954/141‑1 (MQ=255)                           cccTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCt                                                                                              <  1:10080/141‑1 (MQ=255)                                ggAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGa                                                                                         <  1:206731/141‑1 (MQ=255)                                     gCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATAttt                                                                                    >  1:632322/1‑141 (MQ=255)                                     gCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATAttt                                                                                    >  1:491112/1‑141 (MQ=255)                                     gCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATAttt                                                                                    >  1:332383/1‑141 (MQ=255)                                        aaGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAAt                                                                                 >  1:151510/1‑141 (MQ=255)                                               tCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCt                                                                          <  2:152593/141‑1 (MQ=255)                                                cAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTTAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCtt                                                                         <  1:215325/141‑1 (MQ=255)                                                       aaGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGCTGTTGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGttt                                                                     >  1:181242/1‑141 (MQ=255)                                                       aaGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTAc                                                                  <  1:514077/141‑1 (MQ=255)                                                       aaGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTAc                                                                  <  1:313838/141‑1 (MQ=255)                                                             tGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTataatgtt                                                            >  1:159291/1‑134 (MQ=255)                                                               tataAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACATATATGc                                                          >  2:31910/1‑141 (MQ=255)                                                                 taAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCg                                                        <  1:571135/141‑1 (MQ=255)                                                                  aaaTAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGa                                                       <  2:192387/141‑1 (MQ=255)                                                                    aTAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTTTTTTTTTATTTTCAAACATCTTTTCTATTATTTGTTATTCTTGTTTAGATTTAATTGTATCTTGTTTTCCAAatatttcgaat                                                     >  2:641368/1‑134 (MQ=12)                                                                       gagaACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGa                                                  <  1:729253/141‑1 (MQ=255)                                                                        agaACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGTGTTTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGAt                                                 >  2:456705/1‑141 (MQ=255)                                                                          aaCAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAg                                               <  1:380906/141‑1 (MQ=255)                                                                                       tatTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTGTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTg                                  <  1:526675/141‑1 (MQ=255)                                                                                        atTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGc                                 >  2:755331/1‑141 (MQ=255)                                                                                           ttCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGCTTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCaat                              >  1:83647/1‑141 (MQ=255)                                                                                           ttCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCaat                              >  1:83453/1‑141 (MQ=255)                                                                                            tCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCaata                             <  1:395530/141‑1 (MQ=255)                                                                                              tAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCTAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCaataat                           <  2:154502/141‑1 (MQ=255)                                                                                                  tGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCt                       <  2:357541/141‑1 (MQ=255)                                                                                                    aaaaTTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTAc                     >  2:553335/1‑141 (MQ=255)                                                                                                       aTTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTgg                  <  2:223903/141‑1 (MQ=255)                                                                                                            cTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAAcc             >  1:697220/1‑141 (MQ=255)                                                                                                            cTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAAcc             <  2:319839/141‑1 (MQ=255)                                                                                                                 tgc‑‑‑tgTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAACCActct        >  2:300494/1‑141 (MQ=255)                                                                                                                   c‑‑‑tgTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAACCACTCTaa      <  2:629511/141‑1 (MQ=255)                                                                                                                         tttttttAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAACCACTCTAAAGa   <  1:553335/141‑1 (MQ=255)                                                                                                                          ttttttAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAACCACTCTAAAGaa  >  1:765129/1‑141 (MQ=255)                                                                                                                          |                                                                                                                                             AGTCGAAGACCGAGGCTGAGACGGCACCCTAGGAAAGCGAAGCCATTCAATACGAAGTATTGTATAAATAGAGAACAGCAGTAAGATATTTTCTAATTGAAAATTATCTTACTGC‑‑‑TGTTTTTTTAGGGATTTATGTCCCAACCTCTTTTATATTATTCGTTATTCCTGTTGATATTTAATTGGATCTTGTTTTACAAATATGCCGACTAGATAGCCTAATATCGTTGCAATAATTGCTACTGGGAACCACTCTAAAGAA  >  CP000730/1443621‑1443879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.