Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 2,289,252 C→A A80S (GCT→TCT)  ybbP ← hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,289,2520CA94.1% 98.1 / ‑0.5 34A80S (GCT→TCT) ybbPhypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (21/11);  total (22/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

CAGCCTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCG  >  CP000730/2289117‑2289389
                                                                                                                                       |                                                                                                                                         
caGCCTTTGAAACCGCTTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGACAATa                                                                                                                                      >  1:325512/1‑141 (MQ=255)
caGCCATTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCCAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGataata                                                                                                                                      <  1:262970/141‑1 (MQ=255)
      ttGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATAcccccc                                                                                                                                <  1:167411/141‑1 (MQ=255)
      ttGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATAcccccc                                                                                                                                >  2:242428/1‑141 (MQ=255)
       tGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCa                                                                                                                               >  1:279172/1‑141 (MQ=255)
       tGAAACCGATTGAATCAATTTCTCTTCATCTCTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCa                                                                                                                               >  1:173447/1‑141 (MQ=255)
       cgAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCa                                                                                                                               >  1:283509/2‑141 (MQ=255)
                       aaTTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCg                                                                                                               <  1:107861/141‑1 (MQ=255)
                       aaTTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCg                                                                                                               >  1:345527/1‑141 (MQ=255)
                          ttCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGaat                                                                                                            <  1:189497/141‑1 (MQ=255)
                              ctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAAtt                                                                                                        >  1:303720/1‑141 (MQ=255)
                              ctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAAtt                                                                                                        <  1:286684/141‑1 (MQ=255)
                              ctTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAAtt                                                                                                        >  1:20492/1‑141 (MQ=255)
                                 cATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTaa                                                                                                     >  1:40069/1‑140 (MQ=255)
                                 cATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAg                                                                                                     >  2:185067/1‑141 (MQ=255)
                                                          ataGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCAt                                                                            >  2:127252/1‑141 (MQ=255)
                                                            aGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATAc                                                                          <  1:137330/141‑1 (MQ=255)
                                                            aGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATAc                                                                          <  1:157268/141‑1 (MQ=255)
                                                              cGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACtt                                                                        >  1:166910/1‑141 (MQ=255)
                                                                ttttAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTAt                                                                      <  2:234858/141‑1 (MQ=255)
                                                                                          gttCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAACATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAata                                            >  2:220556/1‑141 (MQ=255)
                                                                                           gttctatgCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGATGACCAATAACAATTACTAatat                                           >  2:271783/8‑141 (MQ=255)
                                                                                             cTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTAAATAACGATATCGAATAATTTAGATGTTGCAGTAAAGTTCAATATCATACTTATCTGCTGACCAATGACAATTACTAATATca                                         >  2:328173/1‑140 (MQ=255)
                                                                                                     cGTCTAATTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGt                                 >  1:335712/1‑141 (MQ=255)
                                                                                                           aTTTCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGt                           <  1:48346/141‑1 (MQ=255)
                                                                                                             ttCTGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTAt                         >  1:163307/1‑141 (MQ=255)
                                                                                                                tGGTTGGAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGc                      >  2:313425/1‑141 (MQ=255)
                                                                                                                     ggAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAg                 >  1:225697/1‑141 (MQ=255)
                                                                                                                      gAATATTACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGt                <  1:93168/141‑1 (MQ=255)
                                                                                                                        atatTACTATTAAAGAGAATACCCCCCATTGAATAACGATATAGAATCATTTAGATGTTGTAGTCAAGTTCAATATCATTCTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAACTAAGTGTATCGCTTTCGTTc              >  2:17311/1‑141 (MQ=255)
                                                                                                                           ttACTATTAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCt           >  1:11850/1‑141 (MQ=255)
                                                                                                                           taaCTATTAAAAAAAATAACCCCCATTGAATAACGATATCGAATAAATTAAATGTTTCATTCTAGATCAATATCATACTTATTTACTTTCCTATAAAAATTACTAATATCCTTTCAGGTAATTTTATTGCTTTAGTTCCCt           >  2:226559/3‑141 (MQ=255)
                                                                                                                              ctgttAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTaa        <  2:319924/138‑1 (MQ=255)
                                                                                                                                  tAAAGATAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGAc    <  1:8734/141‑1 (MQ=255)
                                                                                                                                    aaGCTAATACCACCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCg  <  2:269024/141‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                         
CAGCCTTTGAAACCGATTGAATCAATTTCTCTTCATCTTTACTATACGTATTAGAAGTATAGCGTTTTAAAAAGCTACCTCTACCAAGTTGTTCTAACGCACGTCTAATTTCTGGTTGGAATATTACTATTAAAGCTAATACCCCCCATTGAATAACGATATCGAATAATTTAGATGTTGCAGTCAAGTTCAATATCATACTTATCTGCTGACCAATAACAATTACTAATATCCCTTTAAGTAATTGTATCGCTTTAGTTCCCTTAAAGACCG  >  CP000730/2289117‑2289389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑