Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,360,799	A→G	G66G (GGT→GGC)	adk ←	adenylate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,360,799	0	A	G	100.0%	60.6 / NA	18	G66G (GGT→GGC)	adk	adenylate kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/7);  total (11/7)

TGACAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATACCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTCTTG  >  CP000730/2360666‑2360934                                                                                                                                      |                                                                                                                                        tgaCAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAg                                                                                                                                  >  1:9104/1‑141 (MQ=255)   aCAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTc                                                                                                                                <  2:303527/141‑1 (MQ=255)           aaTGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTtcatc                                                                                                                        >  2:335028/1‑141 (MQ=255)            aTGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTtcatcc                                                                                                                       >  2:274417/1‑140 (MQ=255)                       aaGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTc                                                                                                            >  2:190339/1‑141 (MQ=255)                                  taataTTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCTTCTTCTTCAGAAATTCTTTCCTTAACTATGTCTACAGTCACTTCATCAGGAACTAATTCACCACgcgcca                                                                                                 >  2:71095/1‑136 (MQ=255)                                                gCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGTACTAATTCGCCACGGTCCATATAAGAATTAGct                                                                                   >  1:120056/1‑141 (MQ=255)                                                           cTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTaa                                                                        <  2:88168/141‑1 (MQ=255)                                                             cGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTCGCATCGTCTTAAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAAtt                                                                      >  1:209822/1‑141 (MQ=255)                                                                     cTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTttct                                                              <  1:102907/141‑1 (MQ=255)                                                                        gggAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTtcttct                                                           >  1:28264/1‑141 (MQ=255)                                                                        gggAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTtcttct                                                           >  1:205857/1‑141 (MQ=255)                                                                                             ccTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATg                                      <  1:148763/141‑1 (MQ=255)                                                                                                  ttttGCATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCAcc                                 <  1:190339/141‑1 (MQ=255)                                                                                                       cATCGTCTTCAGAAATTCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTg                            <  1:335028/141‑1 (MQ=255)                                                                                                                       tCTTTCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTgg            >  2:93422/1‑141 (MQ=255)                                                                                                                          ttCCTTAACGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGaa         >  2:206175/1‑141 (MQ=255)                                                                                                                                 aCGATGCCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTCTTg  <  1:91425/141‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TGACAGCATCAATGTTTCTGTCAAGCTCAGACATAATATTATTTAATGCCTCAGCTTGCTCGATTGTTCTTGGGAAGCCATCTAATAAAAAGCCTTTTTTTGCATCGTCTTCAGAAATTCTTTCCTTAACGATACCTACAGTCACTTCATCAGGAACTAATTCGCCACGGTCCATATAAGACTTAGCTTCTTTACCTAATTCAGTTTCTTCTTTTATAGCTTTTCTGAACATGTCACCAGTTGAAATGTGGGGTATTGGGAATTTCTTG  >  CP000730/2360666‑2360934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑