Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,709,297	T→A	K544* (AAA→TAA)	oatA ←	possible acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,709,297	0	T	A	100.0%	78.6 / NA	24	K544* (AAA→TAA)	oatA	possible acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (11/13);  total (11/13)

GCGCTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACA  >  CP000730/2709168‑2709427                                                                                                                                  |                                                                                                                                   gcgcTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTAc                                                                                                                         >  2:136021/1‑141 (MQ=255)   gcTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCt                                                                                                                       <  2:134715/141‑1 (MQ=255)      ttACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCa                                                                                                                    <  2:189170/141‑1 (MQ=255)         ctacCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATaa                                                                                                                 >  1:191899/1‑141 (MQ=255)                  tatTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGt                                                                                                        <  2:87267/141‑1 (MQ=255)                            gAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAg                                                                                              >  2:252920/1‑141 (MQ=255)                                    tCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTaaa                                                                                      <  2:298025/141‑1 (MQ=255)                                             aaGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCt                                                                             >  1:280532/1‑141 (MQ=255)                                                   tCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCtttt                                                                       >  1:172456/1‑141 (MQ=255)                                                     cGGATGACCTGCAGAAGCTTTATACCAATCGAATAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTcc                                                                     <  2:59238/141‑1 (MQ=255)                                                            ccTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTa                                                              >  1:69010/1‑141 (MQ=255)                                                                     gCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGt                                                     >  1:260879/1‑141 (MQ=255)                                                                      cTTTATACCAATCGACTAGATGTACAATAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCGTAATCTCTAGGTACTCTAATAGATACTATACATATGTCTGCTTTTCCAAAACTATCCAATAGtt                                                    <  2:109317/141‑1 (MQ=255)                                                                           taCCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCAttt                                               >  2:102250/1‑141 (MQ=255)                                                                              cAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAAt                                            <  2:167927/141‑1 (MQ=255)                                                                              cAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAAt                                            <  2:156317/141‑1 (MQ=255)                                                                               aaTCGACTATATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAAtt                                           <  1:74863/141‑1 (MQ=255)                                                                                               aTTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGc                           >  1:172658/1‑141 (MQ=255)                                                                                                 tAGAGCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGccc                         >  2:346648/1‑141 (MQ=255)                                                                                                  agagCGCTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGcccc                        >  1:310869/1‑141 (MQ=255)                                                                                                     gcgcTTTTCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCAtt                     <  2:286239/141‑1 (MQ=255)                                                                                                          tttCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTAc                <  1:19346/141‑1 (MQ=255)                                                                                                          tttCAGCTGCCTCATAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTAc                <  1:98475/141‑1 (MQ=255)                                                                                                                        tAAATTAATTAATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACa  <  1:97764/141‑1 (MQ=255)                                                                                                                                  |                                                                                                                                   GCGCTTTACTACCTGCATATTCTAAGTGAATACCGTCATATGCAAAGTATTCCGGATGACCTGCAGAAGCTTTATACCAATCGACTAGATGTACATTAGAGCGCTTTTCAGCTGCCTCATAAATTAATTTATTTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACA  >  CP000730/2709168‑2709427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑