Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,440,007 Δ1 bp coding (34/201 nt) cspA ← cold shock protein CspA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,440,0070C.98.3% 546.9 / 1.4 118coding (34/201 nt)cspAcold shock protein CspA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (48/68);  minor base T (0/2);  total (48/70)
Fisher's exact test for biased strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 118 total aligned reads displayed.

CTTGTGGACCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCT  >  CP000730/1439863‑1440151
                                                                                                                                                |                                                                                                                                                
   gtgGACCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATGCGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTaa                                                                                                                                          <  2:364971/149‑1 (MQ=255)
   gtgGACCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTaa                                                                                                                                          <  1:1381148/149‑1 (MQ=255)
   gtgGACCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTaa                                                                                                                                          <  1:651615/149‑1 (MQ=255)
     ggACCGCGGTTGCCTTCAACTACTTCAACCTCAACAGCTTGACCTTCTTCTAATGATTTGTACCCGTCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAAc                                                                                                                                        <  1:826087/149‑1 (MQ=255)
     ggACCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAAc                                                                                                                                        <  1:1665715/149‑1 (MQ=255)
     ggACCGCGGGCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAAc                                                                                                                                        <  1:518556/149‑1 (MQ=255)
     ggACCGCGGCCGCCTTCAACTACTCCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAAc                                                                                                                                        <  1:782099/149‑1 (MQ=255)
            ggTCGCCTTCAACTGCTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTaa                                                                                                                                 <  1:959594/149‑1 (MQ=255)
               cGCCTTCAACTACTTCCACCTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTg                                                                                                                              <  2:1335946/149‑1 (MQ=255)
                 ccTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATCATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTa                                                                                                                            <  2:1433852/149‑1 (MQ=255)
                 ccTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCGAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTa                                                                                                                            <  1:1423515/149‑1 (MQ=255)
                   ttCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTAcc                                                                                                                          >  2:78336/1‑149 (MQ=255)
                      aaCTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTAGCTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTg                                                                                                                       >  2:1046334/1‑149 (MQ=255)
                       actactTCAAACTCAACAGCTTGAGATTGTTTTTATGTTTTGGAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCATTCAACTTCGATAAAGGCGAATCCTTTTTAGCGTTAAACCATTTAACTATACCTTGt                                                                                                                      >  2:371610/1‑149 (MQ=255)
                       actactTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATCGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGt                                                                                                                      >  2:783194/1‑149 (MQ=255)
                         tactTCAGTCGCAACAGCGTGACCTTCTTCCAATGATTTGTAACCCTCTTGGTTAATTGCTGAAAAATGTACTAATACGGCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGttt                                                                                                                    <  2:1697631/149‑1 (MQ=255)
                         tactTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCCCCCTCGATAAATCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGttt                                                                                                                    <  1:1183074/149‑1 (MQ=39)
                         tactTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGATAATTGCTGAAAAACGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGttt                                                                                                                    >  2:917833/1‑149 (MQ=255)
                           ctTCAAGCTCAGCAGCTTGACCTTCTTCTAAGGATTTGTAACCAACTTGGTTAAGGGCTTAAAAATTTACGAATACGTCGTTTTCTGCTTCGACTTCGATAAAGCCGTATCCTTTTTAGCGTTAAACCAGTTAACTGTACCTTGTTTCa                                                                                                                  <  2:643392/149‑1 (MQ=255)
                               aaaCTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAAt                                                                                                              >  2:1670547/1‑149 (MQ=255)
                               aaaCTCAACAGCTTGACCTTCTTCTAATGATGTGTAACCATCTTTGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAAt                                                                                                              <  2:1184044/149‑1 (MQ=255)
                                taCTCACCAGCTTGACCTTCTTCTAACGATTTTTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATc                                                                                                             <  2:129161/148‑1 (MQ=255)
                                  cTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCGTTTTTAGCGTTAAACAATTTAACTGTACCTTGTTTCATAATCTg                                                                                                           >  2:1308641/1‑149 (MQ=255)
                                       cAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGACTACGTCCTTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTGCATAATCGGAAAcc                                                                                                      >  2:391143/1‑149 (MQ=38)
                                        aGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCt                                                                                                     >  1:530291/1‑149 (MQ=255)
                                        aGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCt                                                                                                     >  1:1141381/1‑149 (MQ=255)
                                         gCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACGGTACCTTGTTTCATAATCTGAAACCTc                                                                                                    >  1:641989/1‑149 (MQ=255)
                                           ttGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCa                                                                                                  <  2:595861/149‑1 (MQ=255)
                                            tGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCaa                                                                                                 >  1:592298/1‑149 (MQ=255)
                                              aCCTTCTTCTAATGATTTGTAACCATCTAGGTTAATTGCTGAAAAATGTACGTATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCATGTTAGCGTTAAACCATTTAAATGTACCTTGTTTCATAATCTGAAACCTCCaaaa                                                                                               >  2:946962/1‑147 (MQ=255)
                                                 ttcttcTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTa                                                                                            >  1:616360/1‑149 (MQ=255)
                                                 ttcttcTAATGATTTGTAACCATCTTGGCTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTATTGCGTTAAACCATTTAACTGGACCTTGTTTCATAATCTGAAACCTCCAAGACTa                                                                                            >  2:944734/1‑149 (MQ=255)
                                                  tcttctAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTaa                                                                                           >  2:1563277/1‑149 (MQ=255)
                                                  tcttctAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTaa                                                                                           <  1:1308347/149‑1 (MQ=255)
                                                     tctAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAat                                                                                        <  1:78336/149‑1 (MQ=255)
                                                       tAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCCTTTTCTCCTTCAACTTCGATAAAGCCGAATCCTATTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAatcc                                                                                      >  1:1161165/1‑147 (MQ=255)
                                                         aTGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAattcat                                                                                    >  1:383461/1‑149 (MQ=255)
                                                          tGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTTCTTCATCTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCACCAAAACTAAAattcatt                                                                                   >  2:1503006/1‑149 (MQ=255)
                                                          tGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAattcatt                                                                                   <  2:1335874/149‑1 (MQ=255)
                                                          tGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAattcatt                                                                                   >  2:1298451/1‑149 (MQ=255)
                                                           gATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAattcattc                                                                                  >  1:215352/1‑149 (MQ=255)
                                                           gATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAattcattc                                                                                  >  1:1152809/1‑149 (MQ=255)
                                                           gATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTAGATAAAGCCGAATCCTGTTTAGCGTTAGACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAattcattc                                                                                  >  2:966391/1‑149 (MQ=255)
                                                            aTTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAAttcattca                                                                                 >  2:1319262/1‑149 (MQ=255)
                                                            aTTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAAttcattca                                                                                 >  1:1492680/1‑149 (MQ=255)
                                                                 tAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATg                                                                            <  2:1071247/149‑1 (MQ=255)
                                                                  aaCCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGc                                                                           <  2:1248565/149‑1 (MQ=255)
                                                                     cATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCttt                                                                        >  1:922138/1‑148 (MQ=255)
                                                                       tCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAACCCTCCAAGACTAAAATTCATTCAATATGCTTAtt                                                                      <  2:1492694/149‑1 (MQ=255)
                                                                        cTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTc                                                                     <  2:616360/149‑1 (MQ=255)
                                                                         tttgTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCg                                                                    <  1:1659694/146‑1 (MQ=255)
                                                                         ttGGTTAATTGCTGAAAAATGTACGAATTCGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCg                                                                    <  1:1698659/149‑1 (MQ=255)
                                                                         ttGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCg                                                                    <  2:1609452/149‑1 (MQ=255)
                                                                          tGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGc                                                                   <  1:1328545/149‑1 (MQ=255)
                                                                          tGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGc                                                                   <  1:1439544/149‑1 (MQ=255)
                                                                           ggTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAACCCTCCAAGACTAAAATTCATTCAATATGCTTATTCGcc                                                                  >  1:616403/1‑148 (MQ=255)
                                                                           ggTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCa                                                                  <  1:267713/149‑1 (MQ=255)
                                                                           ggTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCa                                                                  >  2:1505902/1‑149 (MQ=255)
                                                                            gTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCat                                                                 <  1:1379312/149‑1 (MQ=255)
                                                                              tacttGCTGAAAAATGGACGGATACGCCATTTTCTCCTCCAACTTCGATAAACCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCatat                                                               <  1:46828/146‑1 (MQ=255)
                                                                                  tGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAACCCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACa                                                           <  2:564944/149‑1 (MQ=255)
                                                                                  tGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACa                                                           >  2:180476/1‑149 (MQ=255)
                                                                                   gCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCTAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACaa                                                          <  1:742180/149‑1 (MQ=255)
                                                                                     tGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTACACCATTGAACTGTACCTTGTTTCATAATCTGAAACCTCCAGGACTAAAATTCATTCAATATGCTTATTCGCACATTACaaaa                                                        >  2:1058868/1‑149 (MQ=255)
                                                                                     tGAAAAATGTACGAATACGTCATTTTCTACTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGGACCTTGTTTCATAATCTGAAACCACCAAGACTAAAAGTCATTCAATATGCTTAGTCGCACATTACaaag                                                        >  1:1207979/1‑148 (MQ=255)
                                                                                      gAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACaaaaa                                                       <  2:1321239/149‑1 (MQ=255)
                                                                                          aaTGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATAc                                                   <  1:523702/149‑1 (MQ=255)
                                                                                          aaTGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATAc                                                   <  2:953495/149‑1 (MQ=255)
                                                                                                  aaTACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAAt                                           >  1:1301955/1‑149 (MQ=255)
                                                                                                      cGTCAGTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACATCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCa                                       <  2:1254005/149‑1 (MQ=255)
                                                                                                       gTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCAc                                      <  1:441289/149‑1 (MQ=255)
                                                                                                       ctCATTTTCTCCTTCAACTTCGACAAAGCCGAATCCTTTTTAGCGTTAAAACGTTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCAc                                      <  1:48217/148‑1 (MQ=255)
                                                                                                                tcCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATAtt                             <  1:581694/149‑1 (MQ=255)
                                                                                                                tcCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATAtt                             <  1:140570/149‑1 (MQ=255)
                                                                                                                  cgtCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCt                           <  2:456626/147‑1 (MQ=255)
                                                                                                                  cTTCAACTTCGATAACGCTGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCCCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCt                           <  2:2214/149‑1 (MQ=255)
                                                                                                                  cTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTCACTGTTCCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCATTATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCt                           <  1:26808/149‑1 (MQ=255)
                                                                                                                  cTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCt                           <  2:1058874/149‑1 (MQ=255)
                                                                                                                  cTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCt                           <  2:1707984/149‑1 (MQ=255)
                                                                                                                  cTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATGTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTAGAAAAAATACATGCAAATTTCACTCAAATATTCt                           <  1:413170/149‑1 (MQ=255)
                                                                                                                   ttCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAATCCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAACACATGCAAATTTCACTCAAATATTCtt                          <  1:917833/149‑1 (MQ=255)
                                                                                                                   ttCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCtt                          >  1:914733/1‑149 (MQ=255)
                                                                                                                        cTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCaa                     <  1:1624750/149‑1 (MQ=255)
                                                                                                                        cTTCGATAAAGCCCATTCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCaa                     <  1:1504777/149‑1 (MQ=255)
                                                                                                                           tgagaaaGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAACCCTCCAAGACTAAAATTCATTCACTATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAatat                  <  2:256902/145‑1 (MQ=255)
                                                                                                                           cGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAatat                  <  1:292266/149‑1 (MQ=255)
                                                                                                                           cGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAatat                  <  1:464710/149‑1 (MQ=255)
                                                                                                                           cGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGGTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAatat                  <  2:1335615/149‑1 (MQ=255)
                                                                                                                           cGATAAAGCCGAATCCTTTTTAGCGTTAAACCATCTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAatat                  <  1:433808/149‑1 (MQ=255)
                                                                                                                            gATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGGTTCATAATCTGAAACCTCCAAGACTAAACTTCATTGATTATGCTTCTTCCACTATTAGAAAAAATACATGCAAATTTCACTCAAATATTCTTTGACATATg                 >  2:502510/1‑149 (MQ=255)
                                                                                                                             aTAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATgg                <  1:80053/149‑1 (MQ=255)
                                                                                                                                 aGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATTTGGaata            >  1:1321738/1‑149 (MQ=255)
                                                                                                                                 aGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGaata            <  2:1351697/149‑1 (MQ=255)
                                                                                                                                 aGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCAAATTATAAATATTGCTGGCAAATTTCACTCAAATATTCTTTGCAATATGGaata            >  1:131476/1‑149 (MQ=255)
                                                                                                                                   ccGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAACCTACAGGCAAATTTCACTCAAATATTCTTTGCAATATGGaataat          >  1:1327169/1‑149 (MQ=255)
                                                                                                                                   ccGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGaataat          >  2:1352278/1‑149 (MQ=255)
                                                                                                                                      aaTCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAatcat       >  1:891204/1‑149 (MQ=255)
                                                                                                                                      aaTCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAatcat       >  1:106559/1‑149 (MQ=255)
                                                                                                                                           cttttTAACGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAAGAATCATCTGc   <  2:1055886/148‑1 (MQ=255)
                                                                                                                                            ttttTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCt  <  1:1491716/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CTTGTGGACCGCGGTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCT  >  CP000730/1439863‑1440151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: