BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,856,087	T→A	P80P (CCT→CCA)	USA300HOU_2698 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,856,087	0	T	A	99.6%	729.3 / NA	226	P80P (CCT→CCA)	USA300HOU_2698	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (107/117); minor base C (1/0); total (109/117)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 226 total aligned reads displayed.

CTAATGGAGATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCTAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAATTCAAGAACTAT  >  CP000730/2855944‑2856232

ttattGGAGATGCAGCGCCTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACg                                                                                                                                              <  1:1022186/145‑1 (MQ=255)

  aaTGGAGATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTAAAACGGTGTTCGGTCATGCAATCACGAGCCGAAACTTc                                                                                                                                            >  1:1242424/1‑149 (MQ=255)

   aTGGAGATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTca                                                                                                                                           >  1:1070139/1‑149 (MQ=255)

    tGGAGATGCATGGCGTTGGTCCTAAAGCTCTATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTGTTAAGTTAACAGGAGATGTGTCATGCAGTCATGCGCCAAATCGTcaa                                                                                                                                          >  2:1565614/1‑149 (MQ=255)

    tGGAGATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTcaa                                                                                                                                          >  2:254227/1‑149 (MQ=255)

    gggAGATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTcaa                                                                                                                                          <  1:1708773/148‑1 (MQ=255)

     ggAGATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCATCCACCAGCGGCT‑ACAGAAGAGGAGATGTGTCATGCAAGCATGCGCCAAAACGTcaac                                                                                                                                         >  1:813430/1‑149 (MQ=255)

      gagaTGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGACAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTcaaca                                                                                                                                        <  2:1052942/149‑1 (MQ=255)

        gaTGCGTGGCGTTGGTCCTAAAGTTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTAaaaaaa                                                                                                                                      <  2:453183/149‑1 (MQ=255)

            cATGGCGTTGGTCCTCAAGCTATAACAATATTGGAACAAGCTTTATTCCAGCACCAACTACAGATTAAAGCGGTAATTCAGTCAT‑CATAAGATGATGAGATAACAGGGGAAGAGTCATGCACTCATGAGCCAAACCGTCAACAAATGaa                                                                                                                                  >  2:658839/1‑148 (MQ=255)

            cATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGCTGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGAt                                                                                                                                  >  2:1495998/1‑149 (MQ=255)

               ggCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCACCAAATGATAGa                                                                                                                               >  1:309343/1‑149 (MQ=255)

               ggCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGa                                                                                                                               >  1:1183698/1‑149 (MQ=255)

                   ttGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGAtttt                                                                                                                           >  1:922796/1‑149 (MQ=255)

                    tGGCCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTta                                                                                                                          <  2:484923/149‑1 (MQ=255)

                       tCCTAAAGATATAACAATATTGGAACAAGCTTTATTTCAGCACGAACTACATGGTAAAACGGAAGGTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGAGCCAAAACGTCAACAAATGATAGATTTTATAg                                                                                                                       >  2:1438192/1‑149 (MQ=255)

                          tAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTCAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAgttg                                                                                                                    >  2:920076/1‑149 (MQ=255)

                          aacaGCTATATCAATATTAGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAgttg                                                                                                                    <  2:269757/146‑1 (MQ=255)

                           aaaGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAgttgt                                                                                                                   >  1:1227336/1‑149 (MQ=255)

                             aGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTa                                                                                                                 >  1:845346/1‑149 (MQ=255)

                               cTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTAca                                                                                                               <  2:1668877/149‑1 (MQ=255)

                                 atatCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTAcagc                                                                                                             >  2:1314598/1‑149 (MQ=255)

                                   atCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTAcagcag                                                                                                           <  2:865877/149‑1 (MQ=255)

                                   atCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTAcagcag                                                                                                           <  2:313111/149‑1 (MQ=255)

                                    tCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACagcagc                                                                                                          <  2:398647/149‑1 (MQ=255)

                                     cAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAgcagca                                                                                                         >  2:1125909/1‑149 (MQ=255)

                                      aaTATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCAt                                                                                                        <  1:1691677/149‑1 (MQ=255)

                                        tatTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAACATTa                                                                                                      >  1:1274026/1‑149 (MQ=255)

                                         atTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAg                                                                                                     <  1:1581028/149‑1 (MQ=255)

                                         atTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAg                                                                                                     >  1:518832/1‑149 (MQ=255)

                                          ttGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGa                                                                                                    >  1:203345/1‑149 (MQ=255)

                                           tGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGat                                                                                                   >  2:1371697/1‑149 (MQ=255)

                                            ggAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGata                                                                                                  >  2:154098/1‑149 (MQ=255)

                                             gAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAAatat                                                                                                 >  2:1623240/1‑149 (MQ=255)

                                              aaCAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATAtt                                                                                                <  2:1722359/149‑1 (MQ=255)

                                              aaCAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATAtt                                                                                                <  2:235674/149‑1 (MQ=255)

                                                 aaGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATCGTTGTTACAGCAGCATTAGATATTGaa                                                                                             <  1:1032585/149‑1 (MQ=255)

                                                  aGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAat                                                                                            <  2:189850/149‑1 (MQ=255)

                                                   gCTTTATTTCTGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAatt                                                                                           <  2:813430/149‑1 (MQ=255)

                                                   gCTGTAGTTCCGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAatt                                                                                           <  2:599318/149‑1 (MQ=255)

                                                       tATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAAttatta                                                                                       >  1:207050/1‑149 (MQ=255)

                                                             aGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATGGATTTTATAGTTGTTGCAGCAGCATTAGATATTGAATTATTACGTTCa                                                                                 >  1:435169/1‑149 (MQ=255)

                                                              gCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCAc                                                                                <  1:1661148/149‑1 (MQ=255)

                                                                  cAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGt                                                                            <  1:596768/149‑1 (MQ=255)

                                                                   aaCGACATTTTAAAACGGAACTTCAGTCAT‑CATTACCTTTTCAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGtt                                                                           <  2:776668/149‑1 (MQ=255)

                                                                      tACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTaca                                                                        <  1:145071/149‑1 (MQ=255)

                                                                       aCATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTTCAGGAGCATTAGATATTGAATTATTACGTTCACTGGTTacaa                                                                       >  1:741758/1‑149 (MQ=255)

                                                                         ttttgAAAACGGATGTTCAGTCAA‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATTCAATCATGCGCCAAAACGTCAACAATTTATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTacaaca                                                                     <  1:480439/148‑1 (MQ=255)

                                                                         aTTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTacaaca                                                                     <  1:1560480/149‑1 (MQ=255)

                                                                          ttttAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTacaacaa                                                                    >  2:122983/1‑148 (MQ=255)

                                                                          ttttAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAg                                                                    >  1:521830/1‑149 (MQ=255)

                                                                            ttAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGaa                                                                  >  2:555415/1‑149 (MQ=255)

                                                                              aaaaCGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatt                                                                <  2:420144/149‑1 (MQ=255)

                                                                              aaaaCGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatt                                                                >  1:301515/1‑149 (MQ=255)

                                                                                aaCGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAattta                                                              >  2:530371/1‑149 (MQ=255)

                                                                                aaCGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAattta                                                              >  2:824125/1‑149 (MQ=255)

                                                                                  cGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatttatt                                                            >  1:214771/1‑149 (MQ=255)

                                                                                   ggAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatttattt                                                           <  2:720165/149‑1 (MQ=255)

                                                                                   ggAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAACATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAatttattt                                                           >  1:71499/1‑149 (MQ=255)

                                                                                                    ttACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCg                                           <  2:747852/149‑1 (MQ=255)

                                                                                                       ccTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCtt                                        <  1:260549/149‑1 (MQ=255)

                                                                                                       ccTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCtt                                        >  1:1382563/1‑149 (MQ=255)

                                                                                                        cTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCCAACAATCAGCAAATGATGGCAAAAATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCttt                                       >  1:406152/1‑149 (MQ=255)

                                                                                                         ttttAAGTTAACAGGAGATGTTTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGGTTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTg                                      <  1:40222/149‑1 (MQ=255)

                                                                                                         ttttAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTg                                      <  2:1593671/149‑1 (MQ=255)

                                                                                                          tttAAGTTAACAGGAGATGTTTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTACATTATTACGTTCACTGGTTACCACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGa                                     <  2:1266698/149‑1 (MQ=255)

                                                                                                          tttAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGa                                     >  2:1473897/1‑149 (MQ=255)

                                                                                                          tttAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTAGAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGa                                     >  1:1650072/1‑149 (MQ=255)

                                                                                                            tAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACa                                   <  1:966889/149‑1 (MQ=255)

                                                                                                            tAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACa                                   <  1:231839/149‑1 (MQ=255)

                                                                                                               gTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACatat                                >  2:503802/1‑149 (MQ=255)

                                                                                                               gTTAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACatat                                >  2:1572788/1‑149 (MQ=255)

                                                                                                                ttAACAGGGGATGTGTCATGCAATCCTGCGCCAAAACGTCAACTAATGATAGTTTTTTTAGTTGTTACGGCAGCATTAGATATTGAATTATTACGTCCACTGGTTACTACAGAATTTATTTGGTTTGTCCCTGGCCGCTTTGACAtata                               <  2:1369821/149‑1 (MQ=255)

                                                                                                                ttAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAGTTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTATCCCTGGCCGCTTTGACAtata                               >  1:345316/1‑149 (MQ=255)

                                                                                                                gtAACAGCAGGTGAGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTGCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACAtata                               <  1:463063/148‑1 (MQ=255)

                                                                                                                 tAACAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTCTTTGGTCGGTACCTGGCCGCTTTGACATATAc                              >  1:824426/1‑149 (MQ=255)

                                                                                                                   aCAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACgg                            >  1:787528/1‑149 (MQ=255)

                                                                                                                   aCAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACgg                            >  1:1128065/1‑149 (MQ=255)

                                                                                                                   aCAGGAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACgg                            >  1:243216/1‑149 (MQ=255)

                                                                                                                      ggAGATGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGAcc                         >  1:1434398/1‑149 (MQ=255)

                                                                                                                       gagaTGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGActt                        >  1:1671302/1‑147 (MQ=255)

                                                                                                                       gagaTGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCt                        >  1:1310920/1‑149 (MQ=255)

                                                                                                                       gagaTGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCt                        <  1:291483/149‑1 (MQ=255)

                                                                                                                       gagaTGTGTCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCt                        >  1:1428207/1‑149 (MQ=255)

                                                                                                                            gtgtCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAat                   <  2:1355698/149‑1 (MQ=255)

                                                                                                                             tttCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAata                  <  1:1568833/147‑1 (MQ=255)

                                                                                                                             ggtCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAata                  <  2:901090/148‑1 (MQ=255)

                                                                                                                              gtCATGCAATCATGCGCCAAAACGTCCACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAatat                 <  1:1000064/149‑1 (MQ=255)

                                                                                                                              gtCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTCTCCCTGGCCGCTTTGACATATACGGACCTCAAatat                 <  1:1116234/149‑1 (MQ=255)

                                                                                                                              gtCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACCGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAatat                 <  1:1147183/149‑1 (MQ=255)

                                                                                                                              ctCATGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAatat                 <  1:1058807/148‑1 (MQ=255)

                                                                                                                                 aTGCTATCATTCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTaa              <  2:844578/149‑1 (MQ=255)

                                                                                                                                 aTGCAATCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTaa              >  1:173563/1‑149 (MQ=255)

                                                                                                                                    caagcagtcgcCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAATTc           <  2:1026037/141‑1 (MQ=255)

                                                                                                                                     aaTCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAATTCa          <  2:214771/149‑1 (MQ=255)

                                                                                                                                       tCATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAATTCAAg        <  2:1073170/149‑1 (MQ=255)

                                                                                                                                        cATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAATTCAAGa       <  1:1290726/149‑1 (MQ=255)

                                                                                                                                        cATGCGCCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAATTCAAGa       <  1:1345731/149‑1 (MQ=255)

                                                                                                                                            cgcCAAATCGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAATTCAAGAACTa   <  1:1371697/149‑1 (MQ=255)

                                                                                                                                             gcCAAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAATTCAAGAACTAt  <  1:707533/149‑1 (MQ=255)

CTAATGGAGATGCATGGCGTTGGTCCTAAAGCTATATCAATATTGGAACAAGCTTTATTTCAGCACCAACTACATTTTAAAACGGAAGTTCAGTCAT‑CATTACCTTTTAAGTTAACAGGAGATGTGTCATGCAATCATGCGCCTAAACGTCAACAAATGATAGATTTTATAGTTGTTACAGCAGCATTAGATATTGAATTATTACGTTCACTGGTTACAACAGAATTTATTTGGTCTGTCCCTGGCCGCTTTGACATATACGGACCTCAAATATTAATTCAAGAACTAT  >  CP000730/2855944‑2856232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`