Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 622,314 C→A S959R (AGC→AGA)  sdrE → Ser‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730622,3140CA97.8% 387.0 / NA 137S959R (AGC→AGA) sdrESer‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrE
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (84/49);  total (87/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 190 total aligned reads displayed.

TAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGC  >  CP000730/622170‑622458
                                                                                                                                                |                                                                                                                                                
tAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTTGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTgactcagacagcgactcagactcagacagagact                                                                                                                                              <  1:888385/149‑1 (MQ=255)
tAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTgactcagacagcgactcagactcagacagagact                                                                                                                                              <  1:443497/149‑1 (MQ=255)
tAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTgactcagacagcgactcagactcagacagagact                                                                                                                                              <  1:787230/149‑1 (MQ=255)
tAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATGACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTgactcagacagcgactcagactcagacagagact                                                                                                                                              <  1:347770/149‑1 (MQ=255)
   aGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGGTCATGATGCTTTCACCCTTGATAACGGATACTCCGAAGAAGCTCCATCCGACAGCGATTCAGGCACAGATAGTGactcagacagcgcctcagacgccgacagagactcag                                                                                                                                           >  1:981108/1‑149 (MQ=18)
     aTGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACtcagacagcgactcagactcagacagagactcagac                                                                                                                                         <  1:1012697/149‑1 (MQ=255)
      tGCAGATGGTGGCGAAGTTGTCGTAACAATTACGGATCATGAGGATTTCACACATGATAACGGATAGTTCGAAGAAGAGACATCAGACCGCGATTCAGACTCAGATAGTGACTcagacagcgactcagactcagacagagactcagact                                                                                                                                        <  2:179428/149‑1 (MQ=21)
      ggCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTcagacagcgactcagactcagaccgagactccgact                                                                                                                                        <  2:1009549/148‑1 (MQ=255)
       gCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCagacagcgactcagactcagacagagactcagactc                                                                                                                                       <  1:1577076/149‑1 (MQ=255)
        cAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCcgacagggactcagactcagacagagactcagcctcg                                                                                                                                      >  2:1523774/1‑148 (MQ=255)
        cAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCagacagcgactcagactcagacagagactcagcctcc                                                                                                                                      >  1:160185/1‑148 (MQ=255)
        cAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAgacagcgactcagactcagacagagactcagactca                                                                                                                                      >  2:131897/1‑149 (MQ=255)
         aGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGacagcgactcagactcagacagagactcagactcag                                                                                                                                     <  2:166823/149‑1 (MQ=255)
              gtggCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGGTACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCCGACTCAGACAGt                                                                                                                                >  2:1106680/1‑149 (MQ=255)
                ggCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGGAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCagacagcgactgagactcagacagagactcaggctcgggacgtga                                                                                                                              >  2:760056/1‑140 (MQ=21)
                 gCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTgat                                                                                                                             <  2:566211/149‑1 (MQ=255)
                              aaCAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACa                                                                                                                <  1:824892/149‑1 (MQ=255)
                              aaCAATTACGGATCATGATGATTTCACACTTGATAACGGATACTGCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACa                                                                                                                <  2:698898/149‑1 (MQ=255)
                                cAATTACGGATCATGATGATTTCACACTTGATCACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGGGACTCAGACTCGGACAGTGATTCGGATTCAGACAgg                                                                                                              >  1:426448/1‑148 (MQ=255)
                                 aaTTAGTGATCATGATGATTTCACACTGGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACGGTGAttcagattcagagaacg                                                                                                             >  2:378356/1‑144 (MQ=37)
                                 aaTTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACGGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAgag                                                                                                             >  2:121710/1‑147 (MQ=255)
                                 aaTTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCg                                                                                                             >  1:1122925/1‑149 (MQ=255)
                                  atgaCGGATCATGATGATTGCACACTTGAGAACGGATACTTAGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGa                                                                                                            <  2:1193306/146‑1 (MQ=25)
                                  aTTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGa                                                                                                            >  1:266596/1‑149 (MQ=255)
                                    tACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACt                                                                                                          <  1:549253/149‑1 (MQ=255)
                                     aCGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtc                                                                                                         >  1:1391035/1‑149 (MQ=255)
                                     aCGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtc                                                                                                         >  1:796519/1‑149 (MQ=255)
                                      cGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGTGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGAAAGAGACTCAGACTCAGACAGTGATTCAGATTCAGAGAGCGACtcg                                                                                                        >  2:859004/1‑148 (MQ=255)
                                      cGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtca                                                                                                        >  1:1702074/1‑149 (MQ=255)
                                       ggATCATGATGTTGTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGGATCCGCCTACTGTAGTGATTCAGCCAGCGAGCCAGACTCAGACAGCGCCTCAGGATCAGACAGCGATTCAGATTCAGACAGCGACtcag                                                                                                       >  2:438293/1‑149 (MQ=21)
                                       ggATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGGCAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTGAGACTCAGACATTGAttcagattcagagcggagctcgg                                                                                                       >  2:454480/1‑138 (MQ=37)
                                       ggATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcag                                                                                                       >  1:1527857/1‑149 (MQ=255)
                                       ggATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGAAAGCGACtcag                                                                                                       >  1:528896/1‑149 (MQ=255)
                                       ggATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGAAAGTGATTCGGATTCCGACAGCGACtcag                                                                                                       >  1:676775/1‑149 (MQ=255)
                                        gATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACCCACATAGTGACCCCGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGGCAGCGAcacaca                                                                                                      >  2:418025/1‑147 (MQ=25)
                                         aTCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagat                                                                                                     >  1:1559675/1‑149 (MQ=255)
                                         aTCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagat                                                                                                     >  1:1653353/1‑149 (MQ=255)
                                          ccATGATGATTTCCCACTCGCAAACGGCTATTTCGAAGTAGTTTCAGCTGATCGCGAGTCAGTCTGTGATAGTTACCCAGTCAGCGACTCGGACTCAGACGGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagatt                                                                                                    <  2:49674/148‑1 (MQ=12)
                                             tgatgaTTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTAAGACCCAGACAGTGATTCAGATTCCGACAGCGGCtcagattcag                                                                                                 >  2:1442092/1‑149 (MQ=255)
                                             tgatgaTTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtccgaatcaa                                                                                                 >  2:120021/1‑148 (MQ=255)
                                             tgatgaTTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagagtcag                                                                                                 >  2:519299/1‑149 (MQ=255)
                                             tgatgaGTTCGCACGTGATAACGGGTACTTCGACGAAGATACATCAGACAGCGAGTCAGACTCCGATAGTGTCTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagattcag                                                                                                 <  2:129394/149‑1 (MQ=21)
                                                   tatCACACTTGATAACGGAGACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCg                                                                                           <  2:890167/147‑1 (MQ=255)
                                                          cTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCGGATTCAGACAGGGACTCAGATTCAGATAGCGACTCAgg                                                                                    >  2:803782/1‑148 (MQ=255)
                                                             gATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTc                                                                                 <  1:993656/149‑1 (MQ=255)
                                                                  cGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACCCAGACTCAGACAGAGACACAGACACAGACAGTGATTCAGACTCAGACCGAGCCACAGAATCCGAGAGAGACTCTGATTCGGAcc                                                                            >  2:1371356/1‑148 (MQ=25)
                                                                   ggATACTTCGAAGAAGACACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAg                                                                           >  1:753429/1‑149 (MQ=255)
                                                                    gATACTTCGAAGAAGATACATCAGACAGCGATTCAGCCTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACGGCGACTCAGCTTCAGATATAGACTCacgttggtaatgg                                                                          >  1:339079/1‑137 (MQ=21)
                                                                        cTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCATACAGAGACTCAGACTCAGACAGTGAGTCCGATTCAAACTGCGACTCACATTCAGATAGCGCATCAGATTCgaagcgcgctt                                                                      >  2:465018/1‑139 (MQ=25)
                                                                          tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGGCAGCGATTCa                                                                    >  2:918500/1‑149 (MQ=255)
                                                                          tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTcc                                                                    >  1:607127/1‑148 (MQ=255)
                                                                          tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCa                                                                    <  2:1110444/149‑1 (MQ=255)
                                                                          tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCa                                                                    >  2:1488242/1‑149 (MQ=255)
                                                                          tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCa                                                                    >  1:274108/1‑149 (MQ=255)
                                                                          tCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCa                                                                    <  2:257909/149‑1 (MQ=255)
                                                                           cGAAGAAGATACATCAGACGGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAg                                                                   <  2:1313085/149‑1 (MQ=255)
                                                                           cGAAGAAGATACATCAGACAGCAATTCTGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAg                                                                   >  1:949773/1‑149 (MQ=255)
                                                                             aagaagATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGAc                                                                 <  1:193339/149‑1 (MQ=255)
                                                                             aagaagATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGAc                                                                 <  1:1222023/149‑1 (MQ=255)
                                                                               aaacacaccaaacaCAGCGATTCAGACTCAGATAGTGCCTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAAACAGTTATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTc                                                               <  2:339079/136‑1 (MQ=18)
                                                                                aagaTACAGCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACGCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCa                                                              <  2:316700/149‑1 (MQ=255)
                                                                                 agaTACATCAGACAGCGATTCAGCCTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACATTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAg                                                             <  2:1608376/149‑1 (MQ=255)
                                                                                 acaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAg                                                             <  2:426237/147‑1 (MQ=255)
                                                                                  gaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGa                                                            <  2:1163529/149‑1 (MQ=255)
                                                                                  aaTACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGa                                                            <  1:1352869/148‑1 (MQ=255)
                                                                                     aCATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAg                                                         >  1:1488805/1‑149 (MQ=255)
                                                                                     aCATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAg                                                         >  1:1638303/1‑149 (MQ=255)
                                                                                       aTCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTGCGACTCAGGTATCg                                                       >  1:1720226/1‑149 (MQ=18)
                                                                                       aTCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCg                                                       >  1:63936/1‑149 (MQ=255)
                                                                                       aTCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCg                                                       >  2:1538022/1‑149 (MQ=255)
                                                                                         cAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGAGTCGGACAGCTAACCAGACTCAGATAGCGAc                                                     >  1:309842/1‑149 (MQ=18)
                                                                                               gCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagat                                               >  1:1176704/1‑149 (MQ=255)
                                                                                               gCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagat                                               >  1:1020413/1‑149 (MQ=255)
                                                                                                        aCTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCGGAATCGGACTGCGACTCAGACACAGATAGCGACTCAGAGTCAGATGGc                                      >  2:680655/1‑149 (MQ=17)
                                                                                                         gtCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAACGATTCAGACTCAGATAGTGACTCATATTCAGATAGCg                                     <  2:1049452/148‑1 (MQ=255)
                                                                                                         cTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACCCAGACTGGGACAGCTATTCAGACTCAGATAGCGAGTCAGATTCAGATAGCg                                     >  2:1462018/1‑149 (MQ=17)
                                                                                                          tCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                                    >  1:1389037/1‑149 (MQ=255)
                                                                                                              aTAGTGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATtcg                                >  1:602353/1‑148 (MQ=255)
                                                                                                                  tGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCCGATTCAGATAGCGATtcagacc                            >  1:1371777/1‑148 (MQ=255)
                                                                                                                  tGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATtcagagt                            >  2:226549/1‑147 (MQ=255)
                                                                                                                  tGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAATCAGAAAGCGAAtcagaat                            >  2:1581346/1‑147 (MQ=255)
                                                                                                                  tGACTCAGACAGCGACTCAGACTCAGACAGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCCACTCAGATTCAGATAGCGACTCAGAGTCGGACAGCGATTCAGACTCACATAGCGACTCAGCGTCAGATAGCGAAtcagact                            >  2:961128/1‑149 (MQ=17)
                                                                                                                  tGACTCAGACAGCGAATCAGACTCAGACGGAGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATtcagact                            >  1:1079812/1‑149 (MQ=255)
                                                                                                                     ctcagacagcgactcagactcagacagagactcagaCTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATtcagactcag                         <  1:933104/149‑1 (MQ=255)
                                                                                                                      tcagacagcgactcagactcagacagagactcagacTCAGACAGTGATTCAGATTCAGACAGCGACtcagattcag                                                                                                 >  1:1321652/1‑76 (MQ=255)
                                                                                                                      tcagacagcgactcagactcagacagagactcagacTCAGACAGTGATTCAGATTCAGACAGCGACTCATATTCAGATAGCGACGCAGAAGCGGACAGCGAAGCAGACTCAGATAGCCACTCAGATGCAGACAGCGAcacagacacaga                        >  1:357367/1‑149 (MQ=14)
                                                                                                                      tcagacagcgactcagactcagacagagactcagacTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGCTTCAGACTCCGATAGCGACTCAGATTCAGATAGCGATtcagactccga                        >  1:484694/1‑149 (MQ=255)
                                                                                                                      tcagacagcgactcagactcagacagagactcagacTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGAGTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATtcagactcaga                        >  1:376972/1‑149 (MQ=255)
                                                                                                                       cagacagcgactcagactcagacagagactcagactCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAAtcagactcagac                       >  1:508188/1‑149 (MQ=255)
                                                                                                                        agacagcgactcagactcagacagcgactcagactcAGACAGTGATTCAGATTCAGACAGCgactcagactca                                                                                                  <  2:546241/73‑1 (MQ=19)
                                                                                                                        agacagcgactcagactcagacagagactcagactcAGACAGTGATTCAGATTCAGACAGCGACtcagattca                                                                                                  >  1:546241/1‑73 (MQ=255)
                                                                                                                           cagcgactcagactcagacagagactcagactcagaCAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCg                   <  1:454480/149‑1 (MQ=255)
                                                                                                                           cagcgactcagactcagacagagactcagactcagaCAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCGGACCCAGATAGCGATTCAGACCCAGACAGCg                   >  2:1524910/1‑149 (MQ=14)
                                                                                                                               gactcagactcagacagagactcagactcagacagTGATTCAGATTCAGACAg                                                                                                               >  2:1146165/1‑53 (MQ=255)
                                                                                                                                    agactcagacagagactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAAACAGCGACtcagatt          <  2:1713008/149‑1 (MQ=255)
                                                                                                                                       ctcagacagagactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagattcag       >  1:1462049/1‑149 (MQ=255)
                                                                                                                                         cagacagagactcagactcagacagTGATTCAGATGCTGACAGCGACTCAGATTCAGATAGCGACTCAGAATCGGACAGCGATTCAGACTCAGATGGCGACGCAGAATCAGATAGCGATGCAGACTCAGACGGCGACtcagattccgag     >  1:413943/1‑148 (MQ=14)
                                                                                                                                          agacagagactcggactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACtcagatgcaggtg    >  1:25570/1‑146 (MQ=255)
                                                                                                                                          agacagagactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATa    <  1:803782/149‑1 (MQ=255)
                                                                                                                                            acagagactcagactcagacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGc  <  1:1003408/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TAAAGATGCAGATGGTGGCGAAGTTGACGTAACAATTACGGATCATGATGATTTCACACTTGATAACGGATACTTCGAAGAAGATACATCAGACAGCGATTCAGACTCAGATAGTGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGC  >  CP000730/622170‑622458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑