Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 632,405 T→G I177S (ATT→AGT)  proP → MFS family major facilitator transporter, proline/betaine:cation symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730632,4050TG99.0% 677.9 / NA 197I177S (ATT→AGT) proPMFS family major facilitator transporter, proline/betaine:cation symporter
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (110/84);  total (111/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

GTACTACAAGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTT  >  CP000730/632260‑632550
                                                                                                                                                  |                                                                                                                                                 
gTACTACAAGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGc                                                                                                                                                <  2:129243/149‑1 (MQ=255)
gTACTACAAGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGc                                                                                                                                                <  1:1017364/149‑1 (MQ=255)
gTACTACAAGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGc                                                                                                                                                <  2:588372/149‑1 (MQ=255)
gTACTACAAGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGc                                                                                                                                                <  1:607284/149‑1 (MQ=255)
 tactacAAGGGTTTCCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCt                                                                                                                                               <  1:1581399/149‑1 (MQ=255)
  actacAAGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTg                                                                                                                                              >  2:1338781/1‑149 (MQ=255)
    tacAAGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCGGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCGCTGAGGAGTGCTGTa                                                                                                                                            >  1:718759/1‑149 (MQ=255)
    tacAAGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTa                                                                                                                                            >  1:490712/1‑149 (MQ=255)
    tacAAGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTa                                                                                                                                            >  2:832275/1‑149 (MQ=255)
     acAAGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTAt                                                                                                                                           >  2:170390/1‑149 (MQ=255)
       aaGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGACTCATCTACAGAGAAGCGCCGTCTCATGTAAGTTAGTGGACTAGAGGTAGGGACCAATTCAGGTTACATGGCTGCTTCGATTATGAGTGCTGTATTa                                                                                                                                         >  1:1694644/1‑149 (MQ=255)
       aaGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTa                                                                                                                                         >  1:416110/1‑149 (MQ=255)
        aGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGTAATGTGGCCCTTATCGACCACCACGCCGCCTTCAAGTATGAGTGCTGTATTaa                                                                                                                                        >  1:654521/1‑149 (MQ=255)
        aGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTaa                                                                                                                                        >  2:1545523/1‑149 (MQ=255)
        aGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTaa                                                                                                                                        <  1:621665/149‑1 (MQ=255)
        aGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTaa                                                                                                                                        >  1:470094/1‑149 (MQ=255)
             tttCAACAGGTGGAGAGAATGCGGGAGCAATGACATATGTTGCCGAATCATATCCAGATAAGCGTCGTAACTCATTAGGTAGTGGAATAGAAATTGCGACATTATCAGGTTACATAGCTGCTTCATGAATGCTTGCCGTATCaagcttc                                                                                                                                   >  2:1417315/1‑144 (MQ=255)
              ttCAACAGGTGGAGGGTATGCGGGGGCAATGACATATGTTGCCGAATCACCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGCTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCt                                                                                                                                  <  2:889492/149‑1 (MQ=255)
              ttCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCt                                                                                                                                  <  2:329239/149‑1 (MQ=255)
               tCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCtt                                                                                                                                 <  1:1539954/149‑1 (MQ=255)
                cAACATGGGGAGACTATGCCGGGGCAATGACATATTTTGCCGAATCATCTCCATATAAGCTTCGTAACTCATTACGTATTAGACCAGCAATTGCGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCttt                                                                                                                                <  2:948605/149‑1 (MQ=255)
                 aaCAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCCGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGACATTCGCACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCtttt                                                                                                                               <  1:909570/149‑1 (MQ=255)
                 aaCAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTCCATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCtttt                                                                                                                               >  1:1601219/1‑149 (MQ=255)
                 aaCAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCtttt                                                                                                                               <  1:1114418/149‑1 (MQ=255)
                 aaCAGGTGGAGAGCATGCGGGGGTAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCtttt                                                                                                                               <  1:1204225/149‑1 (MQ=255)
                   cAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTa                                                                                                                             <  1:1247478/149‑1 (MQ=255)
                   cAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTa                                                                                                                             <  1:1239687/149‑1 (MQ=255)
                        ggAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGa                                                                                                                        >  1:540502/1‑149 (MQ=255)
                               aTGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACaa                                                                                                                 <  1:1106844/149‑1 (MQ=255)
                               aTGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACaa                                                                                                                 >  2:1252399/1‑149 (MQ=255)
                                 gcgcacgCAAGGACATCTGTTTCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATTAGTGCTGTTTTAACATTCTTTTTAACAGATGAACaaag                                                                                                               >  2:1664597/7‑148 (MQ=255)
                                       gCAATGACATATGTTGCCGAATCATCTCCAGATACGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGcatc                                                                                                         >  2:1218965/1‑149 (MQ=255)
                                             aCATATGTTGCCGAATCATCTCCAGATAGGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATttgg                                                                                                   <  2:142505/149‑1 (MQ=255)
                                              cATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATttggt                                                                                                  >  2:1333236/1‑149 (MQ=255)
                                              cATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATttggt                                                                                                  >  1:1301867/1‑149 (MQ=255)
                                                    ttGCCGAATCATCTGCAGATAAGCGTCGGAACTCAGTAGGTAGTGGACTAGAATTTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGGGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGgaga                                                                                            <  2:1375853/149‑1 (MQ=255)
                                                    ttGCCGAATCATCTCCATATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGTGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGgaga                                                                                            <  2:1052082/149‑1 (MQ=255)
                                                     tGCCGAATCATCTCCAGGTAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTGTTTAACAGCTGAACAAATGGCCTCATTTGGGTGgcgag                                                                                           >  1:448348/1‑148 (MQ=255)
                                                     tGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGaa                                                                                           >  1:1060556/1‑149 (MQ=255)
                                                     tGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGaa                                                                                           >  1:1516998/1‑149 (MQ=255)
                                                      gCCGAATCGTCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAAt                                                                                          <  1:328562/149‑1 (MQ=255)
                                                       ccGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATc                                                                                         >  2:294311/1‑149 (MQ=255)
                                                        cGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTCACAGATGAACAAATGGCATCATTTGGTTGGAGAATcc                                                                                        <  2:369675/149‑1 (MQ=255)
                                                             catcTCCAGATAAGAGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTTTTTTTAACAGATGAACAAATGGCATCATTTGGTTGTAGAATCCCATTc                                                                                   <  1:1230779/149‑1 (MQ=255)
                                                                    aGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCg                                                                            >  1:988444/1‑149 (MQ=255)
                                                                      aTAAGCCTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGt                                                                          <  1:1634794/149‑1 (MQ=255)
                                                                       tcaGCGTCGGAAATCAATAGGTAGTGGACTAGAAATTGGTACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGtt                                                                         <  2:1590302/147‑1 (MQ=255)
                                                                       tAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGtt                                                                         <  1:1422404/149‑1 (MQ=255)
                                                                          gCGTCGTAACTCCTTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGAGGAACAAATGGCATCAATTGGTTGGAGAATCCCATTCTTACTCGGTTTAt                                                                      >  1:1168359/1‑149 (MQ=255)
                                                                          gCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTAt                                                                      >  1:77590/1‑149 (MQ=255)
                                                                           cgtcgtAACTCATTAGGTAGTGGGCTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTAtt                                                                     <  2:1467215/149‑1 (MQ=255)
                                                                           cgtcgtAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTAtt                                                                     >  1:1478855/1‑149 (MQ=255)
                                                                           cgtcgtAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGCTTGGAGAATCCCATTCTTACTCGGCTTAtt                                                                     >  2:1501837/1‑149 (MQ=255)
                                                                            gtcgtAACTCATTAGGTAGTGGACTATAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTTGAGAATCCCATTCTTACTCGGTTTATTc                                                                    <  1:1043241/149‑1 (MQ=255)
                                                                            gtcgtAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTc                                                                    >  1:1367999/1‑149 (MQ=255)
                                                                            gtcgtAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTc                                                                    >  2:54746/1‑149 (MQ=255)
                                                                              cgtAACTCATTAGGTAGTGGACTCGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCt                                                                  >  1:1277379/1‑149 (MQ=255)
                                                                              cgtAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCt                                                                  >  1:699347/1‑149 (MQ=255)
                                                                              cgtAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCt                                                                  >  1:931547/1‑149 (MQ=255)
                                                                                 aaCTCATTAGGTAGTGGACTAGAAATTGGGACCTTATCAGGGTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAgg                                                               <  2:572395/149‑1 (MQ=255)
                                                                                  aCTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGa                                                              >  1:40281/1‑149 (MQ=255)
                                                                                        tAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTGATCGGTTTATTCCTAGAATTTTTc                                                        >  2:1430915/1‑149 (MQ=255)
                                                                                        tAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTc                                                        >  2:152722/1‑149 (MQ=255)
                                                                                          ggTAGTGGACTAGAAATTGGGACATTATCAGGTTCCATAGTTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCgg                                                      <  2:507314/149‑1 (MQ=255)
                                                                                           gTAGTGGACTAGAAATTGGGACGTTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGc                                                     >  2:1172527/1‑149 (MQ=255)
                                                                                           gTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTTATATTCCTGTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGc                                                     >  1:326998/1‑149 (MQ=255)
                                                                                           gTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATAAACATTCTTTTTACCAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGc                                                     >  2:89639/1‑149 (MQ=255)
                                                                                           gTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAACTATGAGTGCTGTATTAACATTCTTTTTAACAGGTGAACAAATGGCAGCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTGGGATTAGTCGGc                                                     >  2:754597/1‑149 (MQ=255)
                                                                                           gTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCGTCAATTATGAGTGCTGTATTAACTTTCTTTTTAACAGATGGCCAAATGGCAGCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTTCTAGTAGTATTCGGc                                                     >  1:223128/1‑149 (MQ=255)
                                                                                            tAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCt                                                    <  2:1366627/149‑1 (MQ=255)
                                                                                              gTGGACTAGAAATTGGGCCATTATCAGGTTACGTAGCTGCTTCAATTTTGAGTGCTGTATTAACATTCTTTTTAACAGATGATCAAATGGTTTCATTTGGTTGGAGAATCCCATTCTTTCTCGGTTTATTCCTAGGATTATTCGGCTta                                                  <  1:766108/149‑1 (MQ=255)
                                                                                              gTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGGATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTta                                                  <  1:109543/149‑1 (MQ=255)
                                                                                                ggACTAGAAATTGGGACATCATCAGGTTAAATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGGTTATTCCTAGGATTATTCGGAttttt                                                >  2:779185/1‑146 (MQ=255)
                                                                                                      gAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTAcg                                          <  1:579498/149‑1 (MQ=255)
                                                                                                      gAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTAcg                                          >  2:637769/1‑149 (MQ=255)
                                                                                                      gAAATTGAGACACTATCAGGTTACATAGCTGCTTCAATGATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTAcg                                          <  2:802992/149‑1 (MQ=255)
                                                                                                       aaaTTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTAcgt                                         <  1:1333236/149‑1 (MQ=255)
                                                                                                        aaTTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTAcgtc                                        >  1:805612/1‑149 (MQ=255)
                                                                                                        aaTTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTAcgtc                                        >  1:1502770/1‑149 (MQ=255)
                                                                                                           tGGGACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTa                                     <  2:60567/149‑1 (MQ=255)
                                                                                                             ggACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTGCGTCGTCAg                                   >  1:1353177/1‑149 (MQ=255)
                                                                                                             ggACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATACGGCTTATATTGACGTCGTAAg                                   >  1:643983/1‑149 (MQ=255)
                                                                                                              gACATTATCATGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGc                                  <  1:170390/149‑1 (MQ=255)
                                                                                                              gACATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACCTCGTAAGc                                  >  2:1106608/1‑149 (MQ=255)
                                                                                                               aCATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCt                                 >  1:1360842/1‑149 (MQ=255)
                                                                                                               aCATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCt                                 >  1:1444752/1‑149 (MQ=255)
                                                                                                               aCATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCt                                 >  1:868429/1‑149 (MQ=255)
                                                                                                               aCATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCt                                 >  1:284365/1‑149 (MQ=255)
                                                                                                                cATTATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTg                                <  1:294311/149‑1 (MQ=255)
                                                                                                                  ttATCAGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGga                              <  1:1015603/149‑1 (MQ=255)
                                                                                                                       aGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTTTTTTTTTTTCAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGCAGAAt                         >  2:1064627/1‑149 (MQ=255)
                                                                                                                       aGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAAt                         >  2:1308031/1‑149 (MQ=255)
                                                                                                                       aGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAAt                         >  1:537319/1‑149 (MQ=255)
                                                                                                                       aGGTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGATGGAAGAAt                         >  2:206369/1‑149 (MQ=255)
                                                                                                                        ggTTACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTAATGCTGATGGTTATTCGGCTTCTATTTACGTCGTAAGCTGGAAGAATc                        >  1:1414675/1‑149 (MQ=255)
                                                                                                                          ttACATAGCTGCTTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCAc                      <  2:1104713/149‑1 (MQ=255)
                                                                                                                                 gctgctTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGACGAATCACCGGcttt               >  2:264393/1‑149 (MQ=255)
                                                                                                                                  ctgctTCGATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGACTCCCATTCTTACTCGGTTTATTCCTGGGATTCTTCGGCTTATATTTACGGCATCAGCTAgcagacaagccacttttc              >  2:1327986/1‑136 (MQ=255)
                                                                                                                                  ctgctTCAATTATGAGTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTTGTAAGCTAGAAGAATCACCAGttttt              >  2:1699415/1‑148 (MQ=255)
                                                                                                                                               tGATTGCTGTAATAACATTCTCTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGtt  <  2:1016950/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTACTACAAGGGTTTTCAACAGGTGGAGAGTATGCGGGGGCAATGACATATGTTGCCGAATCATCTCCAGATAAGCGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTT  >  CP000730/632260‑632550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: