Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,471	A→G	31.0%	S27S (AGT→AGC)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,471	0	A	G	31.0%	70.3 / 44.9	61	S27S (AGT→AGC)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (17/25);  new base G (6/13);  total (23/38)
Fisher's exact test for biased strand distribution p-value = 5.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

CCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAG  >  CP000730/36333‑36603                                                                                                                                           |                                                                                                                                     ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                                                                    >  1:34079/1‑141 (MQ=255) ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                                                                                                    >  2:179853/1‑141 (MQ=255) ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                                                                                    >  2:158763/1‑141 (MQ=25)  cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                   <  2:90214/141‑1 (MQ=21)  cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                                                                   <  2:104693/141‑1 (MQ=21)  cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCAATATTTGATATACAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                                                                                                                   >  2:70455/1‑141 (MQ=11)   aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAGGTTTACACCACGTTCTCTTAATATTTCAGATCTATCACGATAGCTCa                                                                                                                                  >  2:173445/1‑141 (MQ=11)   aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCa                                                                                                                                  <  2:64207/141‑1 (MQ=255)   aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                                                                                  >  1:276836/1‑141 (MQ=21)    tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAACTTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                                                                                 <  2:17872/141‑1 (MQ=11)    tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                                                                                 <  2:55123/141‑1 (MQ=11)     ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGCTCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                                                <  2:119033/141‑1 (MQ=11)     ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                                                <  2:309368/141‑1 (MQ=11)     ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                                                <  2:14262/141‑1 (MQ=11)     ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                                                                                <  1:292311/141‑1 (MQ=11)     ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                <  2:96147/141‑1 (MQ=18)     ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                <  1:89094/141‑1 (MQ=21)     ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                                                                                <  1:70047/141‑1 (MQ=21)      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                               <  1:84090/141‑1 (MQ=11)      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                                                                               <  1:218995/141‑1 (MQ=11)      tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                                                                                                               <  1:239292/141‑1 (MQ=18)       gTAATAAGCCTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                                                                                              <  2:317504/141‑2 (MQ=11)        taataaGCTTTTTTATGCTTTTTCTTCCAAGTTTGATATAAAATTGGGGCATATTCTTGACCCCAACGGTAGATCGTTGAATAATGAACGTTTACACCACGTTCTCTTAATATTACAGATATATCACGATAGCTAAATgca                                                                                                                             >  1:72597/1‑139 (MQ=11)        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                                                                             <  1:284078/141‑3 (MQ=11)        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGGAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGGTAACTCaaggca                                                                                                                             >  2:276472/1‑137 (MQ=255)        taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGta                                                                                                                             <  2:333585/141‑1 (MQ=40)          ataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGtata                                                                                                                           <  1:118692/141‑1 (MQ=40)                   tttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAta                                                                                                                  >  1:66598/1‑141 (MQ=35)                     tATGCTTTTTCCTCCAAATTTGATACAAAATTGGGGCATATTCTTGACCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagt                                                                                                                <  1:258564/141‑1 (MQ=25)                     tATGCTTGTGGTTCTACATTTGCTACAAGAGGGGGGCATATTCTTGAACCCAACGGTTGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagt                                                                                                                <  1:240746/141‑1 (MQ=11)                      aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagta                                                                                                               >  2:236258/1‑141 (MQ=35)                              tcttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACgg                                                                                                       <  2:207615/141‑1 (MQ=35)                               cttcCATATTTGATACAAAATTGGGGCATATTCTTGAACCCACCGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTCATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGc                                                                                                      <  2:108500/141‑1 (MQ=255)                                ttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAATGGCt                                                                                                     >  1:291155/1‑141 (MQ=25)                                 tcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTa                                                                                                    <  2:113635/141‑1 (MQ=35)                                     aaTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGt                                                                                                >  1:118378/1‑141 (MQ=35)                                         tGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATa                                                                                            >  1:323081/1‑141 (MQ=35)                                                     ggggCATATTCTTGAACCCAACGGTAGACCGTTGACTGATGAACGATTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAAGGAAGCCAACGGCTACAGTGATAACATCCTCGTTa                                                                                >  1:172309/1‑141 (MQ=11)                                                     ggggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACCGATCATTTATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTa                                                                                >  1:108224/1‑141 (MQ=11)                                                           tatTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGt                                                                          <  1:125598/141‑1 (MQ=32)                                                            atTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTCCCCTTAATATGTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGtt                                                                         <  2:217931/141‑1 (MQ=17)                                                              tCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTta                                                                       <  1:13723/141‑1 (MQ=32)                                                                 tGAACCCAACGGTAGACCGTTGTATTATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTatat                                                                    <  1:116132/141‑1 (MQ=17)                                                                   aaCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCt                                                                  <  2:102456/141‑1 (MQ=32)                                                                   aaCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCt                                                                  <  2:237456/141‑1 (MQ=32)                                                                    aCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTg                                                                 <  2:4894/141‑1 (MQ=32)                                                                        aaCGGTAGACCGTTGAATGATGAACGTTTACACCCCGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAAt                                                             <  2:172309/141‑1 (MQ=17)                                                                                    ttGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTAATAAaga                                                 >  2:5408/1‑141 (MQ=17)                                                                                      gAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACagaag                                               >  1:7897/1‑141 (MQ=32)                                                                                        atgatgAACGTTTACACCTCGTTCCATTAATATTTCACTTATATCACGATAACTCAATGTATAGCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTCAGATCATAAATAGTTCATACAGAAGAc                                             <  1:161215/141‑1 (MQ=11)                                                                                                 gTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTg                                    <  2:57195/141‑1 (MQ=32)                                                                                                    tACGCCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTa                                 <  2:282617/141‑1 (MQ=17)                                                                                                         cacGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAtt                            >  1:162333/1‑141 (MQ=32)                                                                                                               cccTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACta                      <  2:7897/141‑1 (MQ=32)                                                                                                                      tacttCAGATATATCACGATAACTCAATGCATATCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTc               >  1:304285/4‑141 (MQ=2)                                                                                                                          tctgATAGATCACGATAGAACAACGCATATCTTAGATAGGATCCAACGGCTACAGTTATAACAACCTTTTTAAATTGTGTATATCTGAAATAGTTCATACAGACGACTCCTTTTTGTTAAAAATATATTATAAATTCAACt           <  2:271922/138‑1 (MQ=1)                                                                                                                            aGATATATCACGATAGCTCAATGGATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAACTTCAACttt         <  2:214127/141‑1 (MQ=255)                                                                                                                                atatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGGTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCaa     >  1:210175/1‑141 (MQ=25)                                                                                                                                 tatCACGATAGCTCAATGAATATATTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTAATACAGAAGCCTCCTTTTTATTCAAATTATGTTATAAATTCAACTTTGCaag    >  1:130499/1‑140 (MQ=11)                                                                                                                                  atCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACa   >  1:97397/1‑141 (MQ=35)                                                                                                                                  atCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACa   >  2:272158/1‑141 (MQ=35)                                                                                                                                   acacggcaaCTCAATGCATATCTTAGATAGTAGCCAACAGCTACAGTGACAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAg  <  1:183352/134‑1 (MQ=1)                                                                                                                                           |                                                                                                                                     CCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAG  >  CP000730/36333‑36603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑