Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,265 C→T 24.1% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2650CT24.1% 236.5 / 103.7 190intergenic (+323/‑811)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (63/81);  new base T (31/15);  total (94/96)
Fisher's exact test for biased strand distribution p-value = 6.57e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

Only 100 of 194 total aligned reads displayed.

AAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAA  >  CP000730/76125‑76405
                                                                                                                                            |                                                                                                                                            
aaGGCAATTAATTTAATAATTATCGCAAAATATAGGATCTGTGCCAAAGATGAATCAAGAGGATAATTATAACAAAAAGGAGTCTTCTGTATGAACTATTTAAGATATAAACAATTTAACAAGGATGTTATCAATGTAGcc                                                                                                                                              >  2:37600/1‑141 (MQ=18)
          atttaatAATTATAGCAAAATATAGGTTCTGTTGTAAAGTTGAAGCTATAGTATAATTTTAACAAAAATGAGTGTTTTGTTTGTTTTCTAACGGTTAAAAACACTTTAACAAGGATGTTATCACTGTTGTCGTTGGctact                                                                                                                                    <  2:321297/141‑1 (MQ=12)
                         agtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAt                                                                                                                     <  1:292981/133‑1 (MQ=11)
                          gtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAtt                                                                                                                    >  1:282624/8‑141 (MQ=11)
                          agttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAtt                                                                                                                    <  1:48079/134‑1 (MQ=255)
                           taaatgaGGTTCTGGTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                                                                                                                   <  2:841/135‑1 (MQ=11)
                            aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                                                                                                                  <  1:3634/136‑1 (MQ=9)
                             aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACACGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAttagt                                                                                                                 >  1:21769/5‑138 (MQ=11)
                              aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                                                                                                                <  1:124480/138‑2 (MQ=2)
                               ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGtt                                                                                                               >  1:225046/1‑141 (MQ=38)
                                 aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATTTAAGATATGCATTGAGTTAt                                                                                                             >  2:75770/1‑141 (MQ=0)
                                    ttcgttttcAAAGTTGAATTTATAATATAATTTGAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGt                                                                                                          <  2:327090/133‑1 (MQ=11)
                                     tgtGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGGATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTg                                                                                                         <  2:294745/139‑1 (MQ=255)
                                         ttGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatat                                                                                                     <  1:33090/141‑1 (MQ=35)
                                               aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa                                                                                               <  2:197066/141‑1 (MQ=35)
                                                  tGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGATACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAat                                                                                            >  2:261204/1‑141 (MQ=21)
                                                    aaTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAatat                                                                                          >  2:320067/1‑141 (MQ=35)
                                                     atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt                                                                                         >  2:72567/4‑141 (MQ=0)
                                                     attaataatataatTTTAACAAAAAGGAGTCTTCTGGATGAACTATTTCAGATATAAACAATTTAACCAGGATGTTACCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt                                                                                         <  1:234679/137‑1 (MQ=11)
                                                       tgatatTATAATGTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTaa                                                                                       <  2:63357/139‑1 (MQ=255)
                                                        tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAg                                                                                      <  1:69446/141‑1 (MQ=17)
                                                        tataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAg                                                                                      >  1:219604/1‑141 (MQ=17)
                                                         ataatataatTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAga                                                                                     <  1:291749/141‑2 (MQ=12)
                                                           aatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAgaga                                                                                   <  2:30616/139‑1 (MQ=12)
                                                               taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTTTAATATTGATGCATTGAGTTATCGTGATGTATCTGAAATATTAAGGGAACgg                                                                               >  1:241265/1‑140 (MQ=11)
                                                                aaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtg                                                                              <  1:47139/141‑1 (MQ=12)
                                                                   tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggtg                                                                           <  1:268634/141‑1 (MQ=17)
                                                                   tttAACAAAAAGGAGGCTTGTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATGACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggtg                                                                           >  1:248974/1‑141 (MQ=255)
                                                                       aCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaaa                                                                       <  2:253098/141‑1 (MQ=12)
                                                                         aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACg                                                                     >  1:29551/1‑141 (MQ=17)
                                                                         aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACg                                                                     >  1:139998/1‑141 (MQ=17)
                                                                          aaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGt                                                                    >  2:103198/1‑141 (MQ=17)
                                                                          aaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGt                                                                    >  2:220924/1‑141 (MQ=17)
                                                                          aaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGGGTAAACGt                                                                    >  2:114399/1‑141 (MQ=12)
                                                                           aaaGGAGTCTTCTGTATGCCCTTTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGtt                                                                   >  2:71900/1‑141 (MQ=11)
                                                                              ggAGTCTTCTGTATGAACTATTTCAGATATCAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcat                                                                >  1:316326/1‑141 (MQ=12)
                                                                                aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatca                                                              >  2:311495/1‑141 (MQ=17)
                                                                                 gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTcatcat                                                             >  1:321926/1‑141 (MQ=32)
                                                                                   cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTc                                                           >  1:253189/1‑141 (MQ=32)
                                                                                   cttctGTATGAACTATTTCAAATATATGCTGTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTc                                                           >  2:152950/1‑141 (MQ=11)
                                                                                    ttctGTATGAACTATTTCAGATATAAACATCTTAACATGGTTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTct                                                          <  2:300494/141‑2 (MQ=11)
                                                                                    ttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTGCATCATTCa                                                          <  2:194645/141‑1 (MQ=12)
                                                                                       tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAAGGAACGTGGTGTAAACGTTCATCATTCAACg                                                       >  1:196391/1‑141 (MQ=17)
                                                                                       tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACg                                                       >  1:142130/1‑141 (MQ=17)
                                                                                        gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACga                                                      <  1:113374/141‑2 (MQ=12)
                                                                                        gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACga                                                      >  1:259388/1‑140 (MQ=12)
                                                                                        gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACga                                                      <  2:259388/141‑2 (MQ=12)
                                                                                           tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTCACTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCt                                                   <  1:171568/141‑1 (MQ=11)
                                                                                           tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACgatat                                                   >  2:332324/1‑137 (MQ=17)
                                                                                            gAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTa                                                  <  1:86194/141‑1 (MQ=255)
                                                                                             aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                 <  1:192012/141‑1 (MQ=255)
                                                                                             aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                 <  1:27745/141‑1 (MQ=255)
                                                                                             aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGCTCATCATTCAACGATCTAc                                                 <  1:199899/141‑1 (MQ=11)
                                                                                              aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGCTATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACgacatacc                                                >  1:160274/1‑134 (MQ=11)
                                                                                               cTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGGAATTTTAAGGGAACGTGGTGTAAACGTTACTCATTCAACAGTCTACCg                                               >  1:186541/1‑141 (MQ=11)
                                                                                                tATTTCAGATATAAACCATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGt                                              <  2:129798/141‑1 (MQ=17)
                                                                                                 aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                             <  1:54351/141‑1 (MQ=12)
                                                                                                  tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTg                                            >  1:18255/1‑141 (MQ=17)
                                                                                                    tCAGATATAAACAATTTCACAGGGATTGTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                          >  2:236950/1‑141 (MQ=11)
                                                                                                    tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTCCCGTTggg                                          >  2:215051/1‑141 (MQ=17)
                                                                                                    tCAGATATAAACAATTTAACAAGAATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAATGAGCTATCGTGATATATCCGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                          >  1:272955/1‑141 (MQ=11)
                                                                                                     cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATGTTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTgggg                                         >  1:108976/1‑140 (MQ=255)
                                                                                                       gATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGACTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAAAGTTCATCATTCAACGGTCTACCGTTGGGTTc                                       >  1:30166/1‑141 (MQ=12)
                                                                                                       gATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTTTAAACGTTCATCATTCAACGATCTACCGTTGGGTTc                                       <  1:231100/141‑1 (MQ=11)
                                                                                                        atatAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACAGTTGGGTTCa                                      >  2:39245/1‑141 (MQ=255)
                                                                                                          ataAACAATTTAACAATGATTTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                    <  2:241265/141‑1 (MQ=12)
                                                                                                          ataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAg                                    <  1:60233/141‑1 (MQ=255)
                                                                                                           tgaaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                   >  2:279926/3‑141 (MQ=255)
                                                                                                           taAACAATTTAACAAGGTTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAATTATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGCTCTACCGTTGGGTTCAAGa                                   <  1:200263/141‑1 (MQ=11)
                                                                                                           taAACAATTTAACAAGGATGTTAGCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                   <  1:203460/141‑1 (MQ=255)
                                                                                                             accAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAat                                 <  1:77952/139‑1 (MQ=255)
                                                                                                                aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATg                              <  1:299408/141‑1 (MQ=32)
                                                                                                                 aTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTGGTGTTAAAGAATATGc                             >  2:282193/1‑141 (MQ=255)
                                                                                                                  tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAATGATCTACCGTTGGGTTCAAGAATATGcc                            <  1:214715/141‑1 (MQ=255)
                                                                                                                  tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                            <  2:168945/141‑1 (MQ=255)
                                                                                                                  tttAACAAGGATGTTATCACTGCAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                            <  1:17215/141‑1 (MQ=17)
                                                                                                                     aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                         <  1:783/141‑1 (MQ=12)
                                                                                                                      aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                        >  2:210960/1‑141 (MQ=17)
                                                                                                                      aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                        >  2:199281/1‑141 (MQ=12)
                                                                                                                      aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                        >  2:297838/1‑141 (MQ=255)
                                                                                                                        aaGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAGACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAtt                      >  2:227335/1‑141 (MQ=17)
                                                                                                                          ggATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                    <  2:123914/141‑1 (MQ=32)
                                                                                                                          ggATGTTACCACTGTAGTCGTTTGCTACTATCTAAGATATGCATTGAGCTATCGTTATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                    <  2:148291/141‑1 (MQ=11)
                                                                                                                             tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATATGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGACCCAATTTtata                 >  2:205985/1‑141 (MQ=11)
                                                                                                                             tGTTATCACTGTAGCCGTCGGCTACTATCTAATGTATGCATTGAGCTATCGTTATATATCGGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                 <  1:70728/141‑1 (MQ=11)
                                                                                                                              gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATTTCTGAAATATTAAGAGAACGTGGTATAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                <  1:246588/141‑1 (MQ=11)
                                                                                                                               ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc               >  1:48935/1‑141 (MQ=255)
                                                                                                                                 aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGATTTTGCCCCAATTTTATAACaa             >  1:43220/1‑141 (MQ=255)
                                                                                                                                 aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa             >  1:304234/1‑141 (MQ=25)
                                                                                                                                 aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa             >  1:152830/1‑141 (MQ=25)
                                                                                                                                      tGTAGCCGTGGGCCACTATCTAAGATATGCATTGAGCTATCGTGATAGATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg        <  1:56836/141‑1 (MQ=11)
                                                                                                                                       gTAGCCGTTGGCTACTATCTAAGATTTGCATTTAGCTATCATGATATATCTGAAATAGTAAGAGAACGGGGTGTAAACGTTCATCATGCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg       <  2:275937/141‑1 (MQ=11)
                                                                                                                                       gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg       <  2:155625/141‑1 (MQ=255)
                                                                                                                                        tAGCCGTTGGCTACTATTTAAGATATGCATTGAGCTATCGTGATATACCTGAAATAGTAAGAGAACGTGGTGTCAACGTTCATTATCCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga      <  2:96324/141‑1 (MQ=11)
                                                                                                                                        tAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga      <  2:106967/141‑1 (MQ=255)
                                                                                                                                        aaGCCGGTGACTACTATCTAAAATATGCATTGAGCTATAGTGATATATCTGAAATATAAAGAGAACGTGATGTAAACGTTCATCATTCAAAGATCCACCAATGCGTGCAAGAATATGCCCCAACTTTATATCAAATTTgaa      <  2:250998/140‑3 (MQ=11)
                                                                                                                                         tgCCGTTGGCTACTATTTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAATGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa     <  2:136468/140‑1 (MQ=11)
                                                                                                                                          gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag    <  2:5617/141‑1 (MQ=255)
                                                                                                                                           ccGTTGGCTACTATCTAAGATATGGATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCATTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga   <  2:178012/141‑1 (MQ=11)
                                                                                                                                            cGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaagaa  >  2:173125/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAA  >  CP000730/76125‑76405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: