Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,295 T→C 62.2% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2950TC62.2% 120.0 / 200.5 223intergenic (+353/‑781)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base T (51/32);  new base C (56/81);  total (107/116)
Fisher's exact test for biased strand distribution p-value = 3.54e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 225 total aligned reads displayed.

TATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCG  >  CP000730/76155‑76434
                                                                                                                                            |                                                                                                                                           
 ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGtt                                                                                                                                            >  1:225046/1‑141 (MQ=38)
   aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATTTAAGATATGCATTGAGTTAt                                                                                                                                          >  2:75770/1‑141 (MQ=0)
    ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATc                                                                                                                                         >  1:200037/1‑141 (MQ=34)
      ttcgttttcAAAGTTGAATTTATAATATAATTTGAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGt                                                                                                                                       <  2:327090/133‑1 (MQ=11)
       tgtGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGGATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTg                                                                                                                                      <  2:294745/139‑1 (MQ=255)
           ttGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatat                                                                                                                                  <  1:33090/141‑1 (MQ=35)
                    tGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGATACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAat                                                                                                                         >  2:261204/1‑141 (MQ=21)
                       atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt                                                                                                                      >  2:72567/4‑141 (MQ=0)
                       attaataatataatTTTAACAAAAAGGAGTCTTCTGGATGAACTATTTCAGATATAAACAATTTAACCAGGATGTTACCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt                                                                                                                      <  1:234679/137‑1 (MQ=11)
                         tgatatTATAATGTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTaa                                                                                                                    <  2:63357/139‑1 (MQ=255)
                          tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGGGATATATCTGCAATATTAAg                                                                                                                   >  2:326697/1‑141 (MQ=255)
                          tataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAg                                                                                                                   >  1:219604/1‑141 (MQ=17)
                               tataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAAc                                                                                                              >  2:310478/1‑141 (MQ=17)
                                 taATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTTTAATATTGATGCATTGAGTTATCGTGATGTATCTGAAATATTAAGGGAACgg                                                                                                            >  1:241265/1‑140 (MQ=11)
                                     tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggtg                                                                                                        >  2:88118/1‑141 (MQ=32)
                                     tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggtg                                                                                                        <  1:268634/141‑1 (MQ=17)
                                        aaCAAAAAGGAGTCTCCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaa                                                                                                     <  1:292749/141‑1 (MQ=12)
                                            aaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGt                                                                                                 >  2:103198/1‑141 (MQ=17)
                                            aaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGt                                                                                                 >  2:220924/1‑141 (MQ=17)
                                            aaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGt                                                                                                 <  2:316945/141‑1 (MQ=12)
                                               aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAAGAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcc                                                                                              >  1:47182/1‑140 (MQ=12)
                                                ggAGTCTTCTGTATGAACTATTTCAGATATCAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcat                                                                                             >  1:316326/1‑141 (MQ=12)
                                                     cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTc                                                                                        >  1:253189/1‑141 (MQ=32)
                                                     cttctGTATGAACTATTTCAAATATATGCTGTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTc                                                                                        >  2:152950/1‑141 (MQ=11)
                                                      ttctGTATGAACTATTTCAGATATAAACATCTTAACATGGTTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTct                                                                                       <  2:300494/141‑2 (MQ=11)
                                                      ttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTGCATCATTCa                                                                                       <  2:194645/141‑1 (MQ=12)
                                                         tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAAGGAACGTGGTGTAAACGTTCATCATTCAACg                                                                                    >  1:196391/1‑141 (MQ=17)
                                                         tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACg                                                                                    >  1:142130/1‑141 (MQ=17)
                                                          gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACga                                                                                   >  1:259388/1‑140 (MQ=12)
                                                           tATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGt                                                                                  <  1:44007/141‑1 (MQ=17)
                                                             tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACgatat                                                                                >  2:332324/1‑137 (MQ=17)
                                                              gAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTa                                                                               <  1:86194/141‑1 (MQ=255)
                                                               aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                                              <  1:192012/141‑1 (MQ=255)
                                                                 cTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGGAATTTTAAGGGAACGTGGTGTAAACGTTACTCATTCAACAGTCTACCg                                                                            >  1:186541/1‑141 (MQ=11)
                                                                  tATTTCAGATATAAACCATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGt                                                                           <  2:129798/141‑1 (MQ=17)
                                                                  tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTATCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCCGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                                                           <  1:66594/141‑1 (MQ=11)
                                                                   aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                          <  1:54351/141‑1 (MQ=12)
                                                                    tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTg                                                                         >  1:18255/1‑141 (MQ=17)
                                                                     ttCAGTTATAAACAATTCAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATAGCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTgg                                                                        <  2:255062/141‑1 (MQ=12)
                                                                      tCAGATATAAACAATTTCACAGGGATTGTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                       >  2:236950/1‑141 (MQ=11)
                                                                      tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTCCCGTTggg                                                                       >  2:215051/1‑141 (MQ=17)
                                                                      tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                       <  1:12358/141‑1 (MQ=12)
                                                                      tCAGATATAAACAATTTAACAAGAATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAATGAGCTATCGTGATATATCCGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                       >  1:272955/1‑141 (MQ=11)
                                                                       cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATGTTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTgggg                                                                      >  1:108976/1‑140 (MQ=255)
                                                                         gATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGACTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAAAGTTCATCATTCAACGGTCTACCGTTGGGTTc                                                                    >  1:30166/1‑141 (MQ=12)
                                                                            ataAACAATTTAACAATGATTTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                                                 <  2:241265/141‑1 (MQ=12)
                                                                             tgaaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                                >  2:279926/3‑141 (MQ=255)
                                                                                aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGAATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCCACGATATACCGTTGGGTTACAGAata                                                             >  1:39148/1‑141 (MQ=11)
                                                                                  aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATg                                                           <  1:299408/141‑1 (MQ=32)
                                                                                   aTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTGGTGTTAAAGAATATGc                                                          >  2:282193/1‑141 (MQ=255)
                                                                                    tttAACAAGGATGTTATTACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                         <  2:171248/141‑1 (MQ=255)
                                                                                    tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAATGATCTACCGTTGGGTTCAAGAATATGcc                                                         <  1:214715/141‑1 (MQ=255)
                                                                                    tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                         <  2:175532/141‑1 (MQ=12)
                                                                                    tttAACAAGGATGTTATCACTGCAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                                                         <  1:17215/141‑1 (MQ=17)
                                                                                       aaCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCa                                                      <  1:783/141‑1 (MQ=12)
                                                                                        aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                     >  1:70455/1‑141 (MQ=255)
                                                                                           aGGATGTTATCACTTTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAttt                                                  <  2:79031/141‑1 (MQ=17)
                                                                                            ggATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                                                 <  2:123914/141‑1 (MQ=32)
                                                                                            ggATGTTACCACTGTAGTCGTTTGCTACTATCTAAGATATGCATTGAGCTATCGTTATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                                                 <  2:148291/141‑1 (MQ=11)
                                                                                              aTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                               <  2:231888/141‑1 (MQ=33)
                                                                                               tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATATGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGACCCAATTTtata                                              >  2:205985/1‑141 (MQ=11)
                                                                                                gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATTTCTGAAATATTAAGAGAACGTGGTATAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                             <  1:246588/141‑1 (MQ=11)
                                                                                                gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                             >  1:232545/1‑141 (MQ=255)
                                                                                                 ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                            >  1:48935/1‑141 (MQ=255)
                                                                                                 ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTGCAGGTATATGCCCCGATTTTCTATc                                            >  1:32288/1‑141 (MQ=11)
                                                                                                  tATCACTGTAGCCGTTGGCTACTATCTAAGATATGAAGTGCGTTATTGTGATATATCTGAAGTAGTAAGTGGACGTGGTGTAATCGATCATCATTGAACGGTATACCGTTGGGGTCAAGACACTGCCCCAAATTAatataa                                           >  2:305308/1‑139 (MQ=2)
                                                                                                   aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa                                          >  1:304234/1‑141 (MQ=25)
                                                                                                   aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa                                          >  2:45367/1‑141 (MQ=25)
                                                                                                   aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa                                          >  1:152830/1‑141 (MQ=25)
                                                                                                        tGTAGCCGTGGGCCACTATCTAAGATATGCATTGAGCTATCGTGATAGATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg                                     <  1:56836/141‑1 (MQ=11)
                                                                                                         gTAGCCGTTGGCTACTATCTAAGATTTGCATTTAGCTATCATGATATATCTGAAATAGTAAGAGAACGGGGTGTAAACGTTCATCATGCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                    <  2:275937/141‑1 (MQ=11)
                                                                                                         gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                    <  2:252381/141‑1 (MQ=255)
                                                                                                          tAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                   >  2:155524/1‑141 (MQ=255)
                                                                                                          tAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                   <  2:106967/141‑1 (MQ=255)
                                                                                                           tgCCGTTGGCTACTATTTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAATGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                  <  2:136468/140‑1 (MQ=11)
                                                                                                           agatgTTGGCTATCATCTAAGATATGCATTGAGTTATCGAGATATAACAGAAATAAAAAGGGAACGTGATGTAAACGTTCATCATTCAACGGTCTACCACTGGGTTAAAGAATATGCCCCAATTTTATATaaaaaaaaaaa                                  <  2:116968/137‑8 (MQ=11)
                                                                                                           aGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                  <  1:71900/141‑1 (MQ=255)
                                                                                                           aGCCGTTGGCTACTATCTAAGACATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTGTATATCAAATTTGgaa                                  <  1:164980/141‑1 (MQ=25)
                                                                                                            gctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                 <  2:18255/138‑1 (MQ=25)
                                                                                                            gcggTTTGCTACTATCTAAGCTATGCATTGAGTTATCTGTATATATCTGAAATACTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                 <  1:28032/138‑1 (MQ=11)
                                                                                                            gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                 <  2:5617/141‑1 (MQ=255)
                                                                                                             ccgtgtgttactaTCTAAGTTATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTGGgaaga                                <  2:177990/133‑1 (MQ=11)
                                                                                                             ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                                >  2:196767/1‑141 (MQ=255)
                                                                                                                 tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaaa                            >  2:205628/1‑141 (MQ=35)
                                                                                                                  ggCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                           <  2:198307/141‑1 (MQ=35)
                                                                                                                    ttactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACTATCTCCCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                         <  1:277939/140‑1 (MQ=11)
                                                                                                                    ctactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                         <  1:139460/141‑1 (MQ=255)
                                                                                                                     tactaTCTAAGATATGCATTGGGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                        <  2:134305/141‑1 (MQ=255)
                                                                                                                      actaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                       >  2:70839/1‑141 (MQ=34)
                                                                                                                          acTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaa                   <  1:285922/140‑1 (MQ=21)
                                                                                                                           cTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaa                  <  1:132515/141‑2 (MQ=255)
                                                                                                                           cTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaa                  <  1:19844/141‑2 (MQ=255)
                                                                                                                             aaGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCAGTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAGTTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagc                <  2:248974/141‑4 (MQ=16)
                                                                                                                              aGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTa               >  1:202070/1‑141 (MQ=38)
                                                                                                                              aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct               <  1:179887/141‑5 (MQ=11)
                                                                                                                                atatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATACAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAGAAGCATaaaaaagcttt             >  2:36757/1‑135 (MQ=11)
                                                                                                                                 tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGCAAAAGCATaaaaaaacaaat            >  2:163578/1‑134 (MQ=255)
                                                                                                                                 tatGCATTGAGGTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat            <  2:260125/141‑8 (MQ=0)
                                                                                                                                      aTTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCCTAAATAGCTATTTACaaa       >  1:15748/1‑141 (MQ=255)
                                                                                                                                       ttGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAAt      <  1:88118/141‑1 (MQ=40)
                                                                                                                                            |                                                                                                                                           
TATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCG  >  CP000730/76155‑76434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: