Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,353 G→A 57.5% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,3530GA57.5% 65.3 / 170.5 158intergenic (+411/‑723)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (35/32);  new base A (30/60);  total (65/93)
Fisher's exact test for biased strand distribution p-value = 2.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 159 total aligned reads displayed.

TATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCATTG  >  CP000730/76214‑76493
                                                                                                                                           |                                                                                                                                            
  tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTCACTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCt                                                                                                                                           <  1:171568/141‑1 (MQ=11)
  tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCt                                                                                                                                           >  1:35614/1‑141 (MQ=255)
    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                                                                                                         <  1:27745/141‑1 (MQ=255)
    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTAc                                                                                                                                         <  1:192012/141‑1 (MQ=255)
    aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGCTCATCATTCAACGATCTAc                                                                                                                                         <  1:199899/141‑1 (MQ=11)
      cTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATAATTCAACGGTCTACCg                                                                                                                                       >  1:186587/1‑141 (MQ=17)
       tATTTCAGATATAAACCATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGt                                                                                                                                      <  2:129798/141‑1 (MQ=17)
       tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTATCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCCGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                                                                                                                      <  1:66594/141‑1 (MQ=11)
       tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                                                                                                                      <  2:38071/141‑1 (MQ=12)
        aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATATGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGtt                                                                                                                                     >  2:135091/1‑141 (MQ=17)
        aTTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                                                                                     <  1:54351/141‑1 (MQ=12)
        aTTTCAAATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGtt                                                                                                                                     <  2:148121/141‑1 (MQ=255)
         tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTg                                                                                                                                    >  1:18255/1‑141 (MQ=17)
          ttCAGTTATAAACAATTCAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATAGCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTgg                                                                                                                                   <  2:255062/141‑1 (MQ=12)
          ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTCACTCAGAAATGCATTGAGCTATCGTGCTATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTgg                                                                                                                                   >  2:201525/1‑141 (MQ=11)
           tCAGATATAAACAATTTCACAGGGATTGTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                                                                                                                  >  2:236950/1‑141 (MQ=11)
           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTCCCGTTggg                                                                                                                                  >  2:215051/1‑141 (MQ=17)
           tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                                                  <  1:12358/141‑1 (MQ=12)
           tCAGATATAAACAATTTAACAAGAATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAATGAGCTATCGTGATATATCCGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                                                                                                  >  1:272955/1‑141 (MQ=11)
            cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATGTTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTgggg                                                                                                                                 >  1:108976/1‑140 (MQ=255)
             aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                                                                                                >  1:294839/1‑141 (MQ=12)
             aGATATAAACAAATTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATGTCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGtt                                                                                                                                >  1:172040/1‑141 (MQ=12)
              gATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGACTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAAAGTTCATCATTCAACGGTCTACCGTTGGGTTc                                                                                                                               >  1:30166/1‑141 (MQ=12)
              gATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTTTAAACGTTCATCATTCAACGATCTACCGTTGGGTTc                                                                                                                               <  1:231100/141‑1 (MQ=11)
               atatAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACAGTTGGGTTCa                                                                                                                              >  2:39245/1‑141 (MQ=255)
                 ataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAg                                                                                                                            <  1:60233/141‑1 (MQ=255)
                 ataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCGACCGTTGGGTTCAAg                                                                                                                            <  1:247652/141‑1 (MQ=255)
                  tgaaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                                                                                           >  2:279926/3‑141 (MQ=255)
                  taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGa                                                                                                                           <  2:248678/141‑1 (MQ=255)
                  taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACAATCTAACGTTGGGTTCAAGa                                                                                                                           >  1:217892/1‑141 (MQ=11)
                    accAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAat                                                                                                                         <  1:77952/139‑1 (MQ=255)
                    aaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAat                                                                                                                         <  2:282624/141‑1 (MQ=17)
                     aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGAATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCCACGATATACCGTTGGGTTACAGAata                                                                                                                        >  1:39148/1‑141 (MQ=11)
                       aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATg                                                                                                                      <  1:299408/141‑1 (MQ=32)
                       aaTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATg                                                                                                                      <  2:297772/141‑1 (MQ=255)
                        aTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGc                                                                                                                     >  1:198307/1‑141 (MQ=17)
                         tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAATGATCTACCGTTGGGTTCAAGAATATGcc                                                                                                                    <  1:214715/141‑1 (MQ=255)
                         tttAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                                                                                    <  2:168945/141‑1 (MQ=255)
                         attAACAAAGATGTTATCACTGTAGCCTGTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcc                                                                                                                    <  2:273049/140‑1 (MQ=11)
                             aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                                >  1:85398/1‑141 (MQ=255)
                             aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                                                                                                >  2:199281/1‑141 (MQ=12)
                              cAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAt                                                                                                               >  2:64227/1‑141 (MQ=12)
                               aaGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAGACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAtt                                                                                                              >  2:227335/1‑141 (MQ=17)
                               aaGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGGATATGCCCCAAtt                                                                                                              >  1:55106/1‑141 (MQ=11)
                                 ggATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                                                                                                            <  2:123914/141‑1 (MQ=32)
                                  gATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAATGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTta                                                                                                           >  2:119253/1‑141 (MQ=17)
                                  gATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTta                                                                                                           >  1:145367/1‑141 (MQ=17)
                                   atggtATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATACATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                                                                                          <  2:74019/137‑1 (MQ=255)
                                   aTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                                                                                          <  1:20057/141‑1 (MQ=255)
                                    tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATATGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGACCCAATTTtata                                                                                                         >  2:205985/1‑141 (MQ=11)
                                    tGTTATCACTGTAGCCGTCGGCTACTATCTAATGTATGCATTGAGCTATCGTTATATATCGGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                                                                                         <  1:70728/141‑1 (MQ=11)
                                     gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATTTCTGAAATATTAAGAGAACGTGGTATAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat                                                                                                        <  1:246588/141‑1 (MQ=11)
                                       tATCACTGTAGCCGTTGGCTACTATCTAAGATATGAAGTGCGTTATTGTGATATATCTGAAGTAGTAAGTGGACGTGGTGTAATCGATCATCATTGAACGGTATACCGTTGGGGTCAAGACACTGCCCCAAATTAatataa                                                                                                      >  2:305308/1‑139 (MQ=2)
                                        aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGATTTTGCCCCAATTTTATAACaa                                                                                                     >  1:43220/1‑141 (MQ=255)
                                        aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa                                                                                                     >  1:152830/1‑141 (MQ=25)
                                             tGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg                                                                                                <  1:301958/141‑1 (MQ=255)
                                              gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                                                                                               <  2:252381/141‑1 (MQ=255)
                                               tAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                                                                              <  2:106967/141‑1 (MQ=255)
                                               tAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                                                                                              >  2:155524/1‑141 (MQ=255)
                                               aaGCCGGTGACTACTATCTAAAATATGCATTGAGCTATAGTGATATATCTGAAATATAAAGAGAACGTGATGTAAACGTTCATCATTCAAAGATCCACCAATGCGTGCAAGAATATGCCCCAACTTTATATCAAATTTgaa                                                                                              <  2:250998/140‑3 (MQ=11)
                                                tgCCGTTGGCTACTATTTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAATGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                                                             <  2:136468/140‑1 (MQ=11)
                                                aGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGGGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                                                             <  2:332761/141‑1 (MQ=21)
                                                aGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaa                                                                                             <  1:71900/141‑1 (MQ=255)
                                                 gctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                                                            <  2:18255/138‑1 (MQ=25)
                                                 gctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTAAAGAATATGCCCCAATTTTATATAGAATTTGgaag                                                                                            >  2:317973/4‑141 (MQ=255)
                                                 gcggTTTGCTACTATCTAAGCTATGCATTGAGTTATCTGTATATATCTGAAATACTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                                                                                            <  1:28032/138‑1 (MQ=11)
                                                  ccgtgtgttactaTCTAAGTTATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTGGgaaga                                                                                           <  2:177990/133‑1 (MQ=11)
                                                  ccGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaaga                                                                                           >  2:196767/1‑141 (MQ=255)
                                                   cGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaagaa                                                                                          >  2:173125/1‑141 (MQ=255)
                                                    gTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaa                                                                                         >  1:179853/1‑141 (MQ=255)
                                                    gTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAAAGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaa                                                                                         >  1:83110/1‑141 (MQ=255)
                                                     ttGGCTACTATCTAATATATGCATTGAGTTATCGTGATATATCTGACATATTGAGGGAACGTGGTGTAAACGTTCATCATTCAACGGACTATCGTGGGGTCTAAGAATATGCCCCAATCTTATATCAAATTTGGAAGaaaa                                                                                        <  2:85266/141‑1 (MQ=11)
                                                      tGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGaaaaa                                                                                       >  2:205685/1‑141 (MQ=35)
                                                      tGGCTACTATCTAAGATACGGATTGAGTTATCGTGATATATCTGAAATATTAAGGGACCGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTAAAGAATATGCCCCAATTTTATATCAAATTTGTAAGaaaaa                                                                                       <  2:76845/141‑1 (MQ=11)
                                                       ggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAATAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                                                                                      <  2:304936/141‑1 (MQ=255)
                                                       ggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAg                                                                                      >  2:154676/1‑141 (MQ=255)
                                                        gCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGc                                                                                     <  1:230211/141‑1 (MQ=255)
                                                         ctactaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                                                    >  2:233416/1‑141 (MQ=35)
                                                         ctactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                                                    <  1:139460/141‑1 (MQ=255)
                                                         ctactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCa                                                                                    <  1:44959/141‑1 (MQ=255)
                                                          tactaTCTAAGTTATGCATTGGGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTTCCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                   <  2:22858/141‑1 (MQ=11)
                                                          tactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGGATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                   <  2:55106/141‑1 (MQ=11)
                                                          tactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                   <  1:132576/141‑1 (MQ=255)
                                                          tactaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCAt                                                                                   <  1:87865/141‑1 (MQ=255)
                                                           actaTCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATa                                                                                  >  2:70839/1‑141 (MQ=34)
                                                              aTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaa                                                                               >  2:179887/1‑141 (MQ=255)
                                                                cTACGATATGCATGGAGCCATCGTCATATATCTGAAATATTAAGAGACCGTGTTGTAAACGTTCATCATTCAACGATCCACCGTTGGGTGCAAGAATATGCCACAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaa                                                                             <  1:324599/141‑2 (MQ=11)
                                                                cTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaa                                                                             <  1:233345/141‑2 (MQ=255)
                                                                   aGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                                                          <  2:176513/141‑5 (MQ=17)
                                                                   aGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTa                                                                          >  1:202070/1‑141 (MQ=38)
                                                                   aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct                                                                          <  1:179887/141‑5 (MQ=11)
                                                                      tatGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGCAAAAGCATaaaaaaacaaat                                                                       >  2:163578/1‑134 (MQ=255)
                                                                      tatGCATGGAGTTCTCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATAATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAAtt                                                                       <  2:132527/141‑1 (MQ=255)
                                                                      aatGCATAGAGCTATCGTGAAATATCCGAAATATTAAGAGAACGTGGGGTAAACGTTCATCATTCAACGATCAACCGTTGGGTTCAAGAATATGCCCCAATTTTAAATCAAATAAAAAAAAAAAAGCATaaaaaagcttat                                                                       <  2:274839/140‑8 (MQ=11)
                                                                           aTTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCCTAAATAGCTATTTACaaa                                                                  >  1:15748/1‑141 (MQ=255)
                                                                            ttGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAAt                                                                 <  1:88118/141‑1 (MQ=40)
                                                                                gTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACAGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAACAAGCATAAAAAACTAATTACAAATGGCg                                                             >  2:327087/1‑141 (MQ=255)
                                                                                              tCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGt                                               <  1:320067/141‑1 (MQ=40)
                                                                                                                              tcatcaTTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTta               >  1:186666/1‑141 (MQ=40)
                                                                                                                                           gTCTACTGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAAGAAAAGGAAAATGGAGCTATTTATATCGTGCCATTg  <  2:250888/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTACAAATGGCGTATTGATGAGACGTACATCAAAATAAAAGGAAAATGGAGCTATTTATATCGTGCCATTG  >  CP000730/76214‑76493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: