Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	478,502	C→A	100%	D342E (GAC→GAA)	ndhF →	NADH dehydrogenase (ubiquinone)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	478,502	0	C	A	100.0%	83.2 / NA	29	D342E (GAC→GAA)	ndhF	NADH dehydrogenase (ubiquinone)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/15);  total (14/15)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATC  >  CP000730/478366‑478638                                                                                                                                         |                                                                                                                                         ttCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCtt                                                                                                                                      >  1:295063/1‑141 (MQ=255)   cAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTAt                                                                                                                                    >  1:38355/1‑141 (MQ=255)        tgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCCTCTGCTAAAGAAGCTTATGGCTg                                                                                                                               >  1:292686/1‑141 (MQ=255)          cATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGa                                                                                                                             >  1:123851/1‑141 (MQ=255)                gtgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCa                                                                                                                       <  1:207359/141‑1 (MQ=255)                gtgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCa                                                                                                                       <  1:29721/141‑1 (MQ=255)                    tttttCAGCATCTGTTATTTATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATCTTCTAAGACAAGCATCTGCTAATGTAGCTTATGGCTGGATTGTCATggg                                                                                                                   <  2:152556/138‑1 (MQ=255)                    atatTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATggg                                                                                                                   <  1:188157/141‑1 (MQ=255)                       ttCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCTTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACg                                                                                                                <  1:132879/141‑1 (MQ=255)                             agcGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTAtt                                                                                                          >  2:101435/1‑141 (MQ=255)                                gATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCCTGGGACGTGTATTAGc                                                                                                       >  2:13148/1‑141 (MQ=255)                                 attattAATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCt                                                                                                      <  2:144168/141‑1 (MQ=255)                                                     cGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTATATCGTGAAGCGATTTCATTTTTGAGAACAAGCAGCGGTGTAAGAATCTTATGGCGGGGTTGTCATAGGACGTGTATTAGCTATTATCCTGGCATTTCTAtt                                                                                  >  1:147636/1‑141 (MQ=255)                                                      ggTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGGGAAGCGATTCAATATTCCCAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAttt                                                                                 <  1:39598/141‑1 (MQ=255)                                                      ggTATTTTAGAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAttt                                                                                 <  1:309763/141‑1 (MQ=255)                                                         aTTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg                                                                              <  2:268638/141‑1 (MQ=255)                                                            tttAAAGCAACATTATTTTCACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCTTGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATg                                                                           <  2:219158/141‑1 (MQ=255)                                                              tAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGGGTATTAGCTCTTATCGTGGCATTTCTATTTTGGATggg                                                                         >  2:243360/1‑139 (MQ=255)                                                                        ttattTTTACAATCAGGGTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGa                                                               <  2:123851/141‑1 (MQ=255)                                                                                    tCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATTGATGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTCTTTTGGGTGAGTAGTGTCAGGAGGGCATATGag                                                   >  2:106398/1‑140 (MQ=255)                                                                                       ggTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGGGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgtg                                                >  1:124527/1‑141 (MQ=255)                                                                                           cTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTaa                                            >  1:86078/1‑141 (MQ=255)                                                                                              tCGGGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTg                                         <  1:155440/141‑1 (MQ=255)                                                                                               tgttaaGTGATTCAATATGCCAAACAAAGCATCTGTTCAAGAAGTTTATGTCTTGATTGTCTTGGGACGTGTATTAGCTATGATCGTGGCATTTCTATTTTGGATGAGTAGTTACAGAAGTGCATATGAAGTTTTAAGTGc                                        <  2:303663/137‑1 (MQ=255)                                                                                                  gAATCGATTTAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACt                                     <  2:128868/141‑1 (MQ=255)                                                                                                                          aGCATCTTCTAAAGAAGCTTATGGCTTGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGt             <  2:295063/141‑1 (MQ=255)                                                                                                                          aGCATCTGCTAAAGAAGCTTATGGCTGGCTTGTCCTGGGACGTGAATTAGGTATTCCGCTGGCATTTCTATTTTGGATGAGAAGTGACAGAAGTGCATCTGAAGTGTTAAGTGCACTACTTCTAGGATGTTAATGACTTGt             >  1:63385/1‑141 (MQ=255)                                                                                                                           gCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTa            >  2:73939/1‑141 (MQ=255)                                                                                                                                    aaaGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAAt   >  2:31207/1‑141 (MQ=255)                                                                                                                                     aagaagCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAATTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATGCTAGGATGGTCATACCTAGTAACTTGGaaac  >  2:227348/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                         TTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATC  >  CP000730/478366‑478638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑