| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 614,181 | T→C | 19.6% | D831D (GAT→GAC) | sdrC → | Ser‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 614,181 | 0 | T | C | 19.6% | 65.1 / 2.9 | 39 | D831D (GAT→GAC) | sdrC | Ser‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrC |
| Reads supporting (aligned to +/- strand): ref base T (19/10); new base C (2/6); total (22/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.54e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACGACTC > CP000730/614041‑614321 | gactcagatccaGAGAGCGACTCAGACTCAGATACCGATTCGGACTCAGACACTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTgattcaga‑t < 2:267121/141‑1 (MQ=21) tcagactcagaTAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTgattcaga‑ttca < 1:33033/141‑1 (MQ=255) agacccagaTAGCGATGCAAATTCAGACAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGAttcaga‑ttcaga < 1:20362/141‑1 (MQ=255) gactcagaTAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATCCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGAGTCAGA‑TTCAGAc > 1:207469/1‑141 (MQ=255) aGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACtca > 1:132762/1‑141 (MQ=255) gCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGGGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGGGATTCAGA‑TTCCGACAGCGCCtcag > 1:67099/1‑141 (MQ=255) cGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTAAGATTCAGACTCAGCTAGCGACTCGGATTCTGACGGCGATTCAGACTCAGACAGCGACTCAGAGTCAGACAGTGATTCAGA‑GTCCGACAGCGACtgaga > 1:108671/1‑141 (MQ=21) cagattcagatCGCGACTCGGACTCAGACTGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACtcagattcag < 2:171246/141‑1 (MQ=255) attcagatAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATtcagata > 1:55247/1‑141 (MQ=255) tccgcaCTCAGACAGTGATTCAGATTCACACACAGATAGCCACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATtcagatagtgactcagactc < 2:259855/136‑1 (MQ=17) ggACTCAGACCGTGACTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATtcagatagcgactcagactcaga < 1:292712/141‑1 (MQ=255) gacccAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACagcgactcagactcagatagcgactcagactcagat > 2:25168/5‑141 (MQ=255) aCTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATtcagatagcgacccagactcagata > 1:181085/1‑141 (MQ=255) aCTCAGACAGAGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGTTtcagatagcgactcagactcagaaa > 1:244751/1‑139 (MQ=255) gACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGCCAGCGATTCAGACTCAGACAGCGACTCAGAATCAGACAGTGATTCAGA‑TGCAGACAGCGACTCAGATtcagatagcgactcagactccgatagccgc > 1:25974/1‑138 (MQ=17) aGTGATTCAGATTCAGCCTCAGATAGCGACTCAGATTCTGATAGCGAGTCAGACTCAGACAGCGACCCAGACCCAGACAGGGATTCAGA‑TTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgactca > 2:316402/1‑141 (MQ=17) aCTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATtcagatagcgactcagactcagatagcgacccagactcagatagcgacc > 2:1163/1‑140 (MQ=255) agggACTCAGATTCGGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCATACTCAGATAGCGACTCAGACTCg < 1:291566/138‑1 (MQ=255) gCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATtcagatagcgactcagactcagatggagaatcagactcaggtagcgattcagtacggg > 2:192434/1‑135 (MQ=17) ggcAGATTCTGATAGCGATTCAGACTCTGATCGCGAGTCAGGCTCAGACAGTGATTCAGC‑TTCAGACAGCGACTCAGCTTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAg < 2:2652/139‑1 (MQ=14) ttCTGACAGCGATTCAGACTCAGACAGCGACTCCGACTCAGACAGTGCTTAACG‑CTACTACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGTCTCAGATAGCGACTCAGAACCGGATAGCgattc > 1:63327/1‑141 (MQ=17) cTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACCGTGCTT‑AGAGCCCATACAGCGCATATGCCGCAGATAGCGACTCAGACTCAGATAACGACCCAGACTCAGATAGCGACTCAGACTCGGATAGCgattcag > 1:329924/1‑141 (MQ=17) cTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgattcag > 2:214529/1‑141 (MQ=255) gACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCgattcagat > 1:216616/1‑141 (MQ=255) cGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCAAttgagattcaga > 1:56925/1‑141 (MQ=255) cGATTCAGACTCAGAAAGTGTTTCGGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGAttcagagtcaga > 2:322901/1‑141 (MQ=14) ttttttttttgacAGCGACTCAGCCTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCCGACTCAGATAGCGACTCAGACTCGGAAAGCGATTCACATGCAGACAg > 2:204209/11‑141 (MQ=17) ctcagacAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtc < 1:150122/141‑1 (MQ=255) atcagacTCCCACCCAGACCCAGACAGCGACTCAGA‑CTCAGACAGCGACACAGATTCAAACACCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCTGATAGCGATTCAGATTCAGACAGCGACtc < 2:328674/140‑1 (MQ=11) accagacACCGAACCACACTCAGACAGCGACCCAGA‑CTCAGACACCGACTCAGATTCAGATACCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtc < 2:328796/139‑1 (MQ=11) cagacAGCGACTCAGACTCAGACCGAGATTCAGA‑CTCAGACAGCGACTCAGATTCAGATAGCACCTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcag < 2:230444/141‑1 (MQ=14) cagacAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcag > 2:331759/1‑141 (MQ=255) cagacAGCGACTCAGACTCAGACAGCGACTCCGA‑CTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGCCTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcag < 2:230539/141‑1 (MQ=14) aaGCGACTCCGACTGAGACAGTGATTCCGA‑TTCAGACAGCGACTCCGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGGTAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACtcagattc < 2:22817/140‑1 (MQ=17) cgccacccagactcagacAGGGACTCAGA‑CTCAGACACCGACTCAGGTTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCTACTCAGCCTCGGATAGCGATTCAGATTCAGACAGCGACtcagattca < 2:216616/134‑1 (MQ=14) cagactcagacAGTGATTCAGA‑TTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGCCCCAGATTCAGATAGca > 2:29855/1‑140 (MQ=255) agactcagacAGCGATTCAGA‑TTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGa < 1:70195/141‑1 (MQ=255) cgcccagacAGGTATTCAGC‑TCCAGACAGCGACTCAGACTCAGAGAGCGACTCCGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGAt < 2:148453/137‑1 (MQ=14) ccctccgacAGCGACTCCCA‑CTCCGACAGCGACTCAGAATCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGAt < 2:39265/139‑1 (MQ=14) ctcagatCGTGACTCTGA‑ATCAGACGGCGACCCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCCGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTc < 2:74611/141‑1 (MQ=11) gcgattcaga‑ttgaGACAGCGACCCCGCTTCAGATCGCGACTCCGACGCAGATAGCGACGCAGACTCAGATAGCGATTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAg < 2:65/139‑1 (MQ=17) gTGATTCAGA‑TTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATGGCGATTCGGACTCAg > 2:49845/1‑141 (MQ=255) tGATTCAGA‑TTCAGACAGCGACTCAGTTTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACACGGATAGCGATTCCGATTCAGACAGCGACTCAGATTCCGATAGCGATTCGGACTCAGa < 1:202660/141‑1 (MQ=255) a‑ttcagaCAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACGACt > 1:62450/1‑141 (MQ=255) ttcagaCCGCGACTCATAATCTTATATCGACTCAGACTCAGACTGCTCCCCCGACCCAGTCATCCACTAAGATTCGGATAGCGATTCAGGTTCAGACCGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACtc < 2:142415/141‑1 (MQ=2) | GACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGA‑TTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAACGACTC > CP000730/614041‑614321 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |