| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,335,371 | A→G | 21.2% | intergenic (+376/+784) | npdA → / ← USA300HOU_2192 | Sir2 family NAD‑dependent deacetylase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,335,371 | 0 | A | G | 21.2% | 24.7 / 5.3 | 19 | intergenic (+376/+784) | npdA/USA300HOU_2192 | Sir2 family NAD‑dependent deacetylase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (10/5); new base G (2/2); total (12/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.96e-01 | |||||||||||
GATTAAAAATAAACATAAAATAAGTAGAAAAATAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCTTGATTGGATATAGCTTT > CP000730/2335231‑2335504 | aaatAAAAATAAACATAAAATAAGTAGAAAAATAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTa < 2:64170/138‑1 (MQ=255) aaCATAAAATAAGTAGAAAAATAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGAcat > 1:88761/1‑139 (MQ=255) aaaaTAAGTAGAAAAATAAACGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTGTTAAAAGCTGGCCATAGACCTCTatc > 1:201631/1‑141 (MQ=255) aaaaTAAGTAGAAAAATAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTGGCCATAGACCTCTatc > 1:62868/1‑141 (MQ=255) aaGTAGAAAAATAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATACCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGACCCCTATcatcat > 2:77392/1‑141 (MQ=255) aaGTAGAAAAAGAAAAGCTACGAATATTAAAAAATTAGATTTCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGACCTCTATcatcat < 2:113938/141‑1 (MQ=255) gTAGAAAAATAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGACCTCTATCATCatat < 1:316668/141‑1 (MQ=255) aaTAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATCGACCTCTATCATCATATAAATGat > 2:51864/1‑141 (MQ=255) aaaTATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAACATTTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGtttt < 1:260470/140‑1 (MQ=255) aaaaaaTGTGATATCTTGATTCTCAAATTCCGATATTAAACCATTATATTTTTCAGAATATCTTGCATGATCTTCATTATTATTAAAAGCTAGCCATCGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTAtt < 2:81795/141‑1 (MQ=255) ttATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCa > 2:237504/1‑141 (MQ=255) aaaTTCCGATATTAAATCATTATATTTTTCTGTATTTTTTGCATAATCTTCATTATTATTAAAATCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTTTTAATTATATATATTTCaaattt > 2:126850/1‑136 (MQ=255) atCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGACCTCTATCATCATATAAATGATCTATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAAACCtt > 2:53064/1‑141 (MQ=255) aaTCTTCATTATTATTAAAAGCTGGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCtt < 2:62868/141‑1 (MQ=255) aaTCTTCATTATTATTAAAAGCTGGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCtt < 2:201631/141‑1 (MQ=255) tCTTCATTATTTTTAAAAGCTAAACGCAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCTTGa > 1:161232/1‑141 (MQ=255) cTTCATTATTATTAAAAGCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGACATTTCCAAGTAACACTTTAAAGTGGTGAt > 2:335258/1‑141 (MQ=255) attattaAAAGCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCTTGATTGGatat > 1:164902/1‑141 (MQ=255) ttaAAAGCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCTTGATTGGATATAGCt > 1:324258/1‑141 (MQ=255) aaaaGCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCTTGATTGGATATAGCttt < 1:63640/141‑1 (MQ=255) | GATTAAAAATAAACATAAAATAAGTAGAAAAATAAAAGCTACGAATATTAAAAAATTAGATATCTTCATTATCAAATTCCGATATTAAATCATTATATTTTTCAGAATATCTTGCATAATCTTCATTATTATTAAAAGCTAGCCATAGACCTCTATCATCATATAAATGATATATTAAGTTTTTAGTATTGTTAATTATATATATTTCAGAATAATTATCATTCTGATTGATATTTGGAACTAACCCTTTAAAGTCTTGATTGGATATAGCTTT > CP000730/2335231‑2335504 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |