Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 36,378 C→T L58L (TTG→TTA)  tpn ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,3780CT87.8% 1109.6 / 147.6 623L58L (TTG→TTA) tpnIS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (39/36);  new base T (272/269);  total (317/306)
Fisher's exact test for biased strand distribution p-value = 8.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 624 total aligned reads displayed.

AATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGAT  >  CP000730/36246‑36512
                                                                                                                                    |                                                                                                                                      
 atatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaa                                                                                                                                     <  2:316932/135‑1 (MQ=37)
    tCTAATTAATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAGATAAAAtt                                                                                                                                  <  1:546213/135‑1 (MQ=11)
      tAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                >  1:635362/1‑135 (MQ=255)
      tAATGTATGTCCCTCTGTATCAATAGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACACAATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                >  1:407822/1‑135 (MQ=11)
         tgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTTGGGc                                                                                                                             >  1:644542/1‑135 (MQ=255)
          gtatgaCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                                                                                                                            <  2:173048/135‑1 (MQ=255)
           tatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAACTTGGGGCat                                                                                                                           <  1:382422/135‑1 (MQ=255)
              gtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                                                                                                                        >  2:234581/1‑135 (MQ=255)
              gtCCCTCTGTATCAATGGCACGATATAAAAAGCACAATTTTCCTTTTATTTTTATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAGATTGGGGCATAtt                                                                                                                        >  1:28159/1‑135 (MQ=11)
               tCCCTCTGCATCAATGTCACGATATAAATAGCACCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTc                                                                                                                       >  1:173327/1‑135 (MQ=255)
                     tgtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTGTATGCTTTTTCTTCCAAATTTGATATAAAATAGGGGCAATGTCTTGAAc                                                                                                                 >  2:384187/4‑135 (MQ=11)
                       taTCAATGGCACGATATAAATAGCTCTATTTTTCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAccc                                                                                                               <  1:248957/134‑1 (MQ=11)
                       cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAACATTGAGGCATATTCTTGAActc                                                                                                               >  2:69608/1‑133 (MQ=255)
                        aTCAATGGCACGATATAAATAGCTTCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGTTTTTTCTTCCAAATTTGATATATAATTGGGGCATATTCTTGAACCCa                                                                                                              <  1:21831/135‑1 (MQ=11)
                        aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTTGGGCATATTCTTGAACCCa                                                                                                              >  1:584267/1‑135 (MQ=255)
                          cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATTTTCTTGAACCCAAc                                                                                                            >  2:300707/1‑135 (MQ=255)
                           aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTAAACCCAACg                                                                                                           >  2:354078/1‑135 (MQ=19)
                             tGGCACGATATAAATAGCTCCATTTTACTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTTAACCCAACGGt                                                                                                         >  1:41768/1‑135 (MQ=255)
                                cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATGCGTGCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTTTTGAACCCAACGGAAGa                                                                                                      <  1:293568/135‑1 (MQ=11)
                                 aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc                                                                                                            >  2:77309/1‑128 (MQ=25)
                                 aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc                                                                                                            <  1:77309/128‑1 (MQ=21)
                                  cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGAcc                                                                                                    <  1:534457/135‑1 (MQ=18)
                                  cGATATAAATAGCTCCATTTACCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGatc                                                                                                    >  1:523111/1‑133 (MQ=255)
                                    atatAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGt                                                                                                  >  1:35433/1‑135 (MQ=40)
                                      ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTg                                                                                                >  1:494601/1‑135 (MQ=255)
                                      ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGGCGATCGTTg                                                                                                >  2:224566/1‑135 (MQ=11)
                                        aactgactccggtttCCTTTTATTTTGTTGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGaa                                                                                              <  2:281125/123‑1 (MQ=11)
                                         aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAat                                                                                             >  2:438331/1‑135 (MQ=255)
                                         aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAat                                                                                             >  2:58040/1‑135 (MQ=255)
                                             gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                                                                                         <  1:455016/135‑1 (MQ=255)
                                             gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATGTGATATAAAATTGGGGCATATTCTTGAACCCAACGGACGATCGTTGAatgctg                                                                                         >  2:345849/1‑135 (MQ=11)
                                             gCTCCATTTTACTATTAGTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                                                                                         >  2:643807/1‑135 (MQ=11)
                                                 cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCTATCGGTAGACCGTTGAATGATGAACg                                                                                     <  1:133619/135‑1 (MQ=255)
                                                  aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCAGATTCTTGGACCCAACGGTAGATCGTTGAATGATGAACGt                                                                                    <  2:28878/135‑1 (MQ=11)
                                                   ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                   <  2:371942/135‑1 (MQ=255)
                                                   ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                   <  2:266072/135‑1 (MQ=255)
                                                   ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                   <  2:104028/135‑1 (MQ=255)
                                                    tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGttt                                                                                  <  2:501054/135‑1 (MQ=18)
                                                    tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGttt                                                                                  <  1:476130/135‑1 (MQ=255)
                                                    tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGttt                                                                                  <  2:15894/135‑1 (MQ=255)
                                                    tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGttt                                                                                  <  2:645774/135‑1 (MQ=255)
                                                     ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTa                                                                                 >  2:611447/1‑135 (MQ=255)
                                                      tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTac                                                                                <  2:189758/135‑1 (MQ=18)
                                                        cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                              <  1:398784/135‑1 (MQ=21)
                                                        cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                                                                              <  1:313848/135‑1 (MQ=255)
                                                         gtttATTTTGATATACGTCTTATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTATTTAGCACAAAGATGAACGTTTAcacc                                                                             <  1:670356/134‑1 (MQ=11)
                                                          ttttttttGATGTACGTCTCATCAATACGCCATATGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                            >  1:44089/1‑135 (MQ=255)
                                                          tttATTTTGATGTACGTCTCATAAATACGCCATATGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAACATTGGGTCATGGTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                            >  1:5926/1‑135 (MQ=14)
                                                           ttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                           >  1:506057/1‑135 (MQ=255)
                                                           ttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                           <  2:593072/135‑1 (MQ=255)
                                                           ttATTTTGATATACATCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcactac                                                                           >  1:481913/1‑135 (MQ=11)
                                                           ttATTTTGATATACAGCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                           >  1:347738/1‑135 (MQ=11)
                                                            tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCATTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                                                                          >  1:187660/1‑135 (MQ=38)
                                                            tATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                          <  2:112760/135‑1 (MQ=255)
                                                            tATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                          >  1:661627/1‑135 (MQ=255)
                                                            tATTTTGATATACGTCACATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGCGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                          <  2:398797/135‑1 (MQ=11)
                                                             aTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGAAGAACGTTTACACCACGt                                                                         >  2:534252/1‑135 (MQ=255)
                                                             aTTTTGATATACGTCTCATCAATCCGCCATTTGAAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                         >  1:150804/1‑135 (MQ=11)
                                                             aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                         <  1:229377/135‑1 (MQ=255)
                                                             aTTCTGATTTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATTTTGATACAAAATTGGGGCATATTCTTGAACCCACCGGTAGACCGTTGAATGATTAACGTTTACACCACGt                                                                         <  2:449435/135‑1 (MQ=14)
                                                              ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCTAATTTGATACAAAATTGGGGCATACTCTTGAACCCAACGGTAGTCCGTTGTATGATGATCGTTTACACCACGtt                                                                        <  1:376777/135‑1 (MQ=14)
                                                              ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt                                                                        >  1:363099/1‑135 (MQ=25)
                                                              ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt                                                                        <  2:244960/135‑1 (MQ=40)
                                                              ttttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTACATGCCTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                        <  2:634427/135‑1 (MQ=11)
                                                                ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTct                                                                      <  2:209538/135‑2 (MQ=255)
                                                                 tGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCAGATTCTGAAACCCAACGGTAGGCTCGTGAATCAGGAACTTTTacagaccgtcccc                                                                     >  2:241654/1‑125 (MQ=14)
                                                                 tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctc                                                                     >  1:328864/1‑133 (MQ=255)
                                                                  gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAATCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                    <  2:463306/135‑1 (MQ=25)
                                                                   atatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                   <  2:328864/132‑1 (MQ=11)
                                                                       aCGTCTCATTAATACGTCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGAAATATTCTTGAACACAACGGTAGATCGTTGAATGCTGAACGTTTACCCCACGTTCTCTTAata                                                               >  2:411957/1‑135 (MQ=11)
                                                                       aCGTCTCATCAATACGCCATTTGTAATAAGATTTATTATGCTTTTTCTTCCAAATTTGAGATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAata                                                               >  1:334306/1‑135 (MQ=255)
                                                                        cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGATCGTTTACACCACGTTCCCTTAatat                                                              <  1:600921/135‑1 (MQ=16)
                                                                        cGTCTCATCAATACGCAATTTATAATAAGCTTTTTTATACTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAAAGTTTACACCACGTTCCCTtattat                                                              >  1:617429/1‑135 (MQ=11)
                                                                            tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                          >  1:276121/1‑135 (MQ=40)
                                                                              atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                        >  2:493293/1‑135 (MQ=255)
                                                                              atcaATACGCAATTTGTAATAGGCTTTTTTATGCTTTTTCTTCCAGATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCAGTTGCATTCATATTTCAGa                                                        >  2:566222/1‑135 (MQ=11)
                                                                               tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGat                                                       <  1:62248/135‑1 (MQ=255)
                                                                                caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGTACGTTTACATCACGTTCTCTTAATATTTCAGata                                                      <  1:166221/135‑1 (MQ=11)
                                                                                caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGata                                                      <  1:109632/135‑1 (MQ=255)
                                                                                caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAAata                                                      >  2:555648/1‑135 (MQ=255)
                                                                                 aaTACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTACAAATTTGAAATAAAATAAGGGCATATTCTTTAACCCAACGGTAGATCGTTTAATCACGAACGTTTACACCACGTTCTATTCATCTTTCAGatct                                                     >  2:154272/1‑133 (MQ=11)
                                                                                   tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatatat                                                   <  1:91771/135‑1 (MQ=40)
                                                                                     cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGATCCCATCGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                 <  1:84840/135‑1 (MQ=11)
                                                                                     cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCACATATATCa                                                 >  1:555736/1‑135 (MQ=255)
                                                                                      gCCATTTTTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                <  2:127246/135‑1 (MQ=255)
                                                                                       ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTATTATTTCAGATATATCACg                                               <  1:574780/135‑1 (MQ=21)
                                                                                        cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                              >  1:287781/1‑135 (MQ=255)
                                                                                         aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                             <  2:654895/135‑1 (MQ=255)
                                                                                          tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATa                                            <  2:524670/135‑1 (MQ=40)
                                                                                          tttGTAATAACCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                            >  1:467950/1‑135 (MQ=255)
                                                                                            tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATATGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGGTCTCTTAATATTTCAGATATATCACGATagc                                          >  1:242550/1‑133 (MQ=11)
                                                                                             gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagct                                         <  2:474190/135‑4 (MQ=11)
                                                                                               aataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGAATATTCTTAAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGAAATATCACGATAAATCa                                       >  1:120490/1‑135 (MQ=255)
                                                                                                ataaGCTTTTTTATGCTTTTTCTTCCAAATTTTATACAAAATTGGGGCATATTCTTGAACCCATCGGTAGACCGTTGAATGATGAACGTTTACACCACGTGCCCTTAATATTTCAGATATATCACGATAACTCaa                                      <  2:87709/135‑1 (MQ=255)
                                                                                                ataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATAAAAAATTGGGGCATATTCTAGAACACAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                      >  1:198248/1‑135 (MQ=255)
                                                                                                ataaGCTTTTTTATGCTTTTTCTTCCAAATTAGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCaa                                      >  1:100253/1‑135 (MQ=18)
                                                                                                    gCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGta                                  >  1:389955/1‑135 (MQ=37)
                                                                                                    gCTTTTTTATGCTTTTTCTTCCAAATTTGATAAAAAATTGCGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACAACACGTTCTTTTAATATTTCAGATATATCACGATAGCTCAATgca                                  <  1:434816/135‑3 (MQ=11)
                                                                                                       tttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTatat                               >  1:321567/1‑135 (MQ=35)
                                                                                                                         cAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATTTTTCACGATAACTCAATGTATATCTTAGATAGTAGCCAACg             <  1:7130/135‑1 (MQ=21)
                                                                                                                                    |                                                                                                                                      
AATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGAT  >  CP000730/36246‑36512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑