Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,455,692 Δ1 bp 100% coding (1210/1260 nt) USA300HOU_2315 → hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,455,6920G.88.6% 258.5 / 25.0 70coding (1210/1260 nt)USA300HOU_2315hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base . (35/27);  minor base A (4/3);  total (40/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

AATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTT  >  CP000730/2455555‑2455825
                                                                                                                                          |                                                                                                                                     
aaTCAGCAGAATCAGGTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGATGTTCATCAGTTGTAATTGATGCAaaaa                                                                                                                                    <  1:94334/141‑1 (MQ=255)
     gcagAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATAGATGCAcaacaatta                                                                                                                               >  2:171621/1‑138 (MQ=255)
       agAATCAGTTAAACAAACACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGAAGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTATTTGTTGCAaaaaaaaaaaa                                                                                                                             <  2:710402/141‑6 (MQ=255)
       agAAACAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTGATAAATAAGACAGACGGTCATCAGTTGTAATTGATGCAaaaaaattatc                                                                                                                             <  2:318540/141‑6 (MQ=255)
        gAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAaaaaaattatcg                                                                                                                            <  1:424154/141‑7 (MQ=255)
             aGTTAACAAAGCACCACAAAGTGACGATTTAACAAACGATGCAAATGAAGATGAAATAGAGCACTCAATCAATGAGCATGCGTTTGAAGAATAACACAGTCGTTGCGAAAGTGTAATTGATGCAAAAAAATTATCGataaa                                                                                                                      <  2:743978/141‑1 (MQ=255)
                  aaCAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGCCGTTCATCAGTTGTAATTGATGCAAAAAAATTATCCAAAAAACTga                                                                                                                 >  2:21789/1‑141 (MQ=255)
                      aGCACCAAAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTACAAAGAAGACAGACGTTCATCAGTTGTAATCGATGCAAAAAAATTATCGAAAAAACTgaagaa                                                                                                             <  1:555262/141‑1 (MQ=255)
                       gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTgaagaaa                                                                                                            >  1:383499/1‑140 (MQ=255)
                       gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAAc                                                                                                            >  2:587125/1‑141 (MQ=255)
                       gCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGAAAGACCGACAGCAGTTGTAATTGATGCAGAAAAATTATCGAAAAAACTGAAGAAc                                                                                                            >  1:175028/1‑141 (MQ=255)
                          ccacAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATGATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAAAAAAAAAAAAAACTGAAGAACAAt                                                                                                         <  2:709651/141‑1 (MQ=255)
                           cacaAAGTAACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATc                                                                                                        <  2:514938/141‑1 (MQ=255)
                            acaAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCt                                                                                                       <  2:71951/141‑1 (MQ=255)
                              aaaGAGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTc                                                                                                     >  1:526628/1‑141 (MQ=255)
                                aGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCaa                                                                                                   >  1:346906/1‑140 (MQ=255)
                                   gACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATa                                                                                                >  2:534433/1‑141 (MQ=255)
                                    aCGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAAGTATCGAAAACACTGAAGAACAATCTTCAGATaa                                                                                               >  1:274004/1‑141 (MQ=255)
                                     cGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaa                                                                                              <  1:65576/141‑1 (MQ=255)
                                     cGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaa                                                                                              >  1:505645/1‑141 (MQ=255)
                                      gATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATaaaa                                                                                             >  1:206970/1‑141 (MQ=255)
                                                aCGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAataata                                                                                   >  2:739394/1‑141 (MQ=255)
                                                  gATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTAAAGAAAAATCTTCAGATAAAAACAAATAATAAc                                                                                 >  1:262771/1‑141 (MQ=255)
                                                  gATTCAAAGGAAGATGAAATAGAGCAAGCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAAc                                                                                 <  2:133120/141‑1 (MQ=255)
                                                   aTTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACa                                                                                <  2:639484/141‑1 (MQ=255)
                                                     tCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACaaa                                                                              >  2:750263/1‑141 (MQ=255)
                                                      cAAATGAAGATTAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAg                                                                             <  1:505909/141‑1 (MQ=255)
                                                      cAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAg                                                                             >  1:99912/1‑141 (MQ=255)
                                                      cAAAAGAAGATGAAAGAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAg                                                                             <  2:381049/141‑1 (MQ=255)
                                                         aTGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAgcgc                                                                          <  1:357924/141‑1 (MQ=255)
                                                                 gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt                                                                  >  2:245158/1‑141 (MQ=255)
                                                                 gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt                                                                  >  2:576138/1‑141 (MQ=255)
                                                                 gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt                                                                  >  2:576507/1‑141 (MQ=255)
                                                                 gAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGt                                                                  >  2:576516/1‑141 (MQ=255)
                                                                   aaaagagAAATCAACAAAAGAAACGGCGACCTAACAAAAAGACAGACGTTCATTAGTTGTAATTGATGCAAAAAAATTTTCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATCACAAAGCGCTTGCTAGTAc                                                                <  1:312134/138‑1 (MQ=255)
                                                                     tAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGCCAAACTTGATGATCAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACct                                                              <  2:162554/141‑1 (MQ=255)
                                                                     tAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCATGCGAGAAccc                                                              >  1:168030/1‑140 (MQ=255)
                                                                       gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGCTAAAAACAAATAATAACAAAGCGCTTGCTAGTACctct                                                            >  1:70573/1‑141 (MQ=255)
                                                                       gagCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTAGCTAGTACctct                                                            >  2:215780/1‑141 (MQ=255)
                                                                        agCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTGGCTAGTACCGCtt                                                           >  2:673295/1‑141 (MQ=255)
                                                                        agCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCGTGCTAGTACCTCtt                                                           >  1:47117/1‑141 (MQ=255)
                                                                         acgagcagtAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACCAAGCGCTTGCTAGTACCTCTTa                                                          <  2:189002/133‑1 (MQ=255)
                                                                           aagcagtAAAACGACCCGCGACTTATAAAGAAGAAAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaa                                                        <  2:212116/135‑1 (MQ=255)
                                                                            aTCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTaaaa                                                       <  2:646416/141‑1 (MQ=255)
                                                                              cgtaaaaaCAACCGGCGACTTACAAAGAAAACAAACGGTCATCATAAATAATTGATGCAAAAACAATACCGAAAAGACAGAATCACAACCTTCAGATAAAAACAAGTAATAACAAAGCTCTTGCTAGTACCTCTTaaaaat                                                     <  2:274004/137‑2 (MQ=255)
                                                                              cATTAAAAGAACCTGAGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAg                                                     >  2:138325/1‑141 (MQ=255)
                                                                                 tAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAACCTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCACGTGCTAGTACCTCTCAAAAAcata                                                  >  2:365039/1‑140 (MQ=255)
                                                                                  aaaaGAACCTGCGACTAATAAAGAAAACAGACGTTCATCAGTAGAAATAGAAAAAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAgatga                                                 <  2:429024/141‑1 (MQ=255)
                                                                                      aaaCCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCt                                             <  1:158598/140‑1 (MQ=255)
                                                                                         ccTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAAAAAATAATAACAAAGCGCTAGCACGTACCTCTTAAAAAGAGAATGCTAGc                                          >  2:359595/1‑141 (MQ=255)
                                                                                            gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAg                                       <  2:557410/141‑1 (MQ=255)
                                                                                            gCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATGGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTCGCAAg                                       >  2:586966/1‑141 (MQ=255)
                                                                                            gCGACCTAGAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAGAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCGAg                                       <  2:538099/141‑1 (MQ=255)
                                                                                             cGACTGATAAAGAAGACAGACGTTCATCAGTTGTAATGGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTGAAAAAGATCATGCTAGCAAgc                                      <  1:400622/141‑1 (MQ=255)
                                                                                                ttaataAAGAAGACAGACGTTCATCAGTTGCAATCGATGCAAAAAAACTATCGAAAAAACTGAAGAACAAAAATAAGATAAAAACAAATAATAACAAAGCGCTTTCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt                                   <  2:583345/138‑1 (MQ=255)
                                                                                                cTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCt                                   <  1:321379/141‑1 (MQ=255)
                                                                                                 ttATAAATAAGACAGACTTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCtt                                  <  2:612835/141‑1 (MQ=255)
                                                                                                 ttATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTACTAGTACCACTTAAAAAGATGATGCTAGAGAGGGCtt                                  >  1:4894/1‑141 (MQ=255)
                                                                                                         aagaCAGACGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGAAGATGCCAGCAAGCGCTTTTctatac                          <  1:570221/141‑1 (MQ=255)
                                                                                                          agacagacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAACATGATGCTAGCAAGCGCTTTTctatact                         >  1:537871/1‑141 (MQ=255)
                                                                                                            acagacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAGGCGCTTTTctatactat                       >  1:613049/1‑141 (MQ=255)
                                                                                                            acagacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactat                       >  1:148551/1‑141 (MQ=255)
                                                                                                            acagacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTctatactat                       >  1:368960/1‑141 (MQ=255)
                                                                                                            acagacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAAACTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTCAAAAGATGATGCTAGCCAGGGCATTTctatcccat                       >  1:238008/1‑136 (MQ=255)
                                                                                                              agacGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAATACCTCTTAAAAAGATGATGCTAGCAAGCCCTTTTCTATACtatat                     >  2:96330/1‑141 (MQ=255)
                                                                                                                acGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCCGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATActatctat                   >  2:520043/1‑141 (MQ=255)
                                                                                                                 cGTTCATCAGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATAtatt                  >  1:391133/1‑141 (MQ=255)
                                                                                                                    tcatcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATattatt               <  2:99912/141‑1 (MQ=255)
                                                                                                                       atcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAtttt             <  1:476499/141‑1 (MQ=255)
                                                                                                                       atcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAtttt             <  2:505645/141‑1 (MQ=255)
                                                                                                                        tcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTAttttt            >  1:265571/1‑141 (MQ=255)
                                                                                                                        tcaGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTCGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATCCTATATATTAttttt            >  2:98962/1‑141 (MQ=255)
                                                                                                                         caGTTGTAATTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTc           >  1:21740/1‑141 (MQ=255)
                                                                                                                                aaTTGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAtt    >  2:683375/1‑141 (MQ=255)
                                                                                                                                  ttGATGCAAAAAAATTATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAAtttt  <  2:563503/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                     
AATCAGCAGAATCAGTTAAACAAGCACCACAAAGTGACGATTTAACAAACGATTCAAATGAAGATGAAATAGAGCAATCATTAAAAGAACCTGCGACTTATAAAGAAGACAGACGTTCATCAGTTGTAATTGATGCAGAAAAACATATCGAAAAAACTGAAGAACAATCTTCAGATAAAAACAAATAATAACAAAGCGCTTGCTAGTACCTCTTAAAAAGATGATGCTAGCAAGCGCTTTTCTATACTATATATTATTTTTCTAAAATTTT  >  CP000730/2455555‑2455825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: