BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,269	C→T	56.0%	A95T (GCA→ACA)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,269	0	C	T	56.0%	95.9 / inf	750	A95T (GCA→ACA)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (166/163); new base T (184/232); total (351/399)
Fisher's exact test for biased strand distribution p-value = 1.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 752 total aligned reads displayed.

GCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36130‑36409

    ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                                                                                                                         >  2:154391/1‑141 (MQ=17)

    ccTTCGTTGAAGGTGCATGATATGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                                                                                                                         >  1:395855/1‑141 (MQ=12)

     cTTCGTTGAAGGTGCCTGTTCTGTAACTACCTTTTGAGGCTTCCCAGATTGTCTATTGAGTCGTTTGGTGAACGCGTATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                                                                                                                                        <  1:221671/141‑1 (MQ=11)

      ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATg                                                                                                                                       >  1:638136/1‑141 (MQ=32)

         gTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTAGTTGCTTACGCAACCAAATATCTTATGTATGTCCCTCTGTATCAATTGCa                                                                                                                                    >  1:313488/1‑141 (MQ=11)

          ttGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCAc                                                                                                                                   <  2:712840/141‑1 (MQ=32)

             aaGGTGCCTGATCTCTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATACTGAATGATTATCTCGTTGCTTACGCAACCAACTATCTAATGTATGGCCCCCTGCATCAATGGACCGat                                                                                                                                >  2:224457/1‑141 (MQ=11)

              aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCGCTGTATCAATGGCACGata                                                                                                                               <  1:414692/141‑1 (MQ=17)

               ggTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCTACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGatat                                                                                                                              <  2:411797/141‑1 (MQ=17)

               ggTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGatat                                                                                                                              >  2:561109/1‑141 (MQ=32)

               ggTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTAGGTCCCTCTGCATCAATGGCACGatat                                                                                                                              >  2:81777/1‑141 (MQ=17)

               ggTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGataa                                                                                                                              >  1:378702/1‑140 (MQ=255)

                gggCCTGAGCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGAtata                                                                                                                             <  1:361354/139‑1 (MQ=11)

                gTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATATCGATGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGAtata                                                                                                                             <  1:764136/141‑1 (MQ=12)

                    gtgttgtGTAATTATCTTTTTTGGTTTACCAAATTGTTTGATGGGGCGTTTGATAATCGGATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATa                                                                                                                         <  1:157153/135‑1 (MQ=11)

                     tGATCTGTAATTACCTTTTGAGGTTTACCACATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAg                                                                                                                        >  1:497936/1‑141 (MQ=17)

                     tGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAACTaa                                                                                                                        >  2:111835/1‑140 (MQ=17)

                      gATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATTTATGTCCCTCTGTATCAATGGCACGAGATAAATAgg                                                                                                                       >  2:144938/1‑140 (MQ=12)

                      gATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGc                                                                                                                       >  1:489441/1‑141 (MQ=32)

                       aTCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCt                                                                                                                      <  2:638987/141‑1 (MQ=12)

                               ttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTc                                                                                                              >  1:205001/1‑141 (MQ=32)

                               ctACCGTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGACAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTc                                                                                                              <  2:501487/140‑1 (MQ=255)

                                   cTTTTGGGGCTTACCAAATTGTTTAACGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                          <  2:398211/141‑1 (MQ=255)

                                   cTTTTGAGGTTTACCAAATTGTTTAATGCGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCCCGATATAAATAGCTCCATTTTCCttt                                                                                                          >  1:93582/1‑141 (MQ=12)

                                   cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCCAATGTATGTCCCCCTGTATCAATGGCGCGAAATAAATAGCTCCATTTTCCttt                                                                                                          >  2:295217/1‑141 (MQ=11)

                                          ggcttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                   >  1:287541/4‑141 (MQ=17)

                                          ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGTATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                   <  2:175266/141‑1 (MQ=17)

                                          ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTGTCTCATTAttttt                                                                                                   >  2:539726/1‑140 (MQ=255)

                                          ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATATAGATGCTTACGCAACCAAATATATAATGTTTGTCCCTCTGCATCAATGACACGATATAAATAGCTCCTTTATCCTTTTATTTTg                                                                                                   >  2:527649/1‑141 (MQ=11)

                                           gTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGa                                                                                                  >  2:516313/1‑141 (MQ=17)

                                            tttACCAAATTGTTTAATGAGGCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAt                                                                                                 <  1:585556/141‑1 (MQ=17)

                                            tttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAt                                                                                                 <  1:424562/141‑1 (MQ=17)

                                            cttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTTCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCATTGGCCCGATATCAATAGCACCATTTTCCTTTTATTTTGAt                                                                                                 >  1:101110/2‑141 (MQ=11)

                                               aCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTa                                                                                              <  2:654533/141‑1 (MQ=32)

                                                ccAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTAc                                                                                             <  2:482108/141‑1 (MQ=32)

                                                   aaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCCCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                          <  2:248215/141‑1 (MQ=12)

                                                        tttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcatc                                                                                     <  1:751221/141‑1 (MQ=32)

                                                          tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCaa                                                                                   >  1:658460/1‑141 (MQ=17)

                                                             tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                <  1:239044/141‑1 (MQ=12)

                                                              gagaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACg                                                                               <  2:580630/141‑1 (MQ=12)

                                                                 aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCa                                                                            >  2:301734/1‑141 (MQ=32)

                                                                   gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCAtt                                                                          <  2:261466/141‑1 (MQ=32)

                                                                    tttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCAttt                                                                         <  2:728144/141‑1 (MQ=32)

                                                                    tttGATAAACGCATATACAGAATCTTTTACTTGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTTTTTTGATATACATCTCATCAATACGCCAttt                                                                         >  2:549226/1‑141 (MQ=11)

                                                                     ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTg                                                                        >  2:665168/1‑141 (MQ=32)

                                                                      tGATAAACACATATGCTGAATGATTATCGCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATGAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGt                                                                       <  1:688648/141‑1 (MQ=11)

                                                                       gATTAACGCATATGCTGAATGATTACCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTCTAAGCCGCGTTTCATCAATACGCCATTTGta                                                                      <  2:354899/141‑1 (MQ=11)

                                                                       gATAAACGCATAGGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGta                                                                      <  2:623361/141‑1 (MQ=17)

                                                                         tAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaat                                                                    >  1:706053/1‑141 (MQ=17)

                                                                               cATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtt                                                              <  1:734461/141‑1 (MQ=255)

                                                                                 tatTCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTTCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtgtt                                                            <  2:453050/141‑1 (MQ=11)

                                                                                 tatGCTTAATGATTATCTCGTTGCGTACGCAACCAAAATCCTGATGTAGGTGCCGCTGCATCAGTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTGTGTGGACGGCTCATCAGTACGTGATTTGGaagaagttgtt                                                            <  2:274601/141‑6 (MQ=2)

                                                                                 tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATATACGTCTCATCAATACGCCATTGTTAATAAtttttt                                                            >  1:24789/1‑141 (MQ=11)

                                                                                   tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                          <  2:365113/141‑1 (MQ=35)

                                                                                    tcgtgaggATAATCGCGTTGGTTACGCAACAAAATATCTAATGTTTTTCCATCTGAATCAATGGCACGATATAAATAGCTCCTTGTTCCTTTTATTTGGATGTCCGTCTCATCAATACGCCTTTTGTAATAAGCTTTTTTa                                                         <  2:379415/134‑1 (MQ=11)

                                                                                     cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAt                                                        >  1:443008/1‑141 (MQ=38)

                                                                                      tGAATGATTATCTCTTTGCTTACGCAACGAAATATCTAATGTAGGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCGCATCAATACGACATTTGTAATAAGCTTTTTTATg                                                       <  1:172752/141‑1 (MQ=14)

                                                                                      tGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATg                                                       <  1:193249/141‑1 (MQ=255)

                                                                                        aaTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCt                                                     <  1:539726/141‑1 (MQ=40)

                                                                                          tGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttt                                                   <  2:688789/141‑1 (MQ=255)

                                                                                           gATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCGCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                  <  1:722297/141‑1 (MQ=255)

                                                                                           gATTATCTCGTTGCTTACGCAACCAAATATCAAATGTATGTCCCTCTGTATAAATGGCCCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                  >  2:389068/1‑141 (MQ=14)

                                                                                            aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAACTTTTTTATGCttttt                                                 >  1:457470/1‑141 (MQ=255)

                                                                                            aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACCCCTATTGTACTAAGCTTTTTTCTGCttttt                                                 >  1:267679/1‑141 (MQ=14)

                                                                                            aTTATCTCGTTGCTTACGCAACCAAATATATAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGGTGTACTTCTCTTCAATACACGAGTGGTGATAAGCTTTTTTagcagtttt                                                 >  1:5957/1‑133 (MQ=11)

                                                                                             ttATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATTGCTCCATTTTCCTTTTATTTTTATGTACGTCTCCTCAATACGCCATTGGTGATCAGCGTTTTACTGCTTTttc                                                >  1:550986/1‑141 (MQ=11)

                                                                                              tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTTTGCTTTttct                                               >  1:675258/1‑141 (MQ=255)

                                                                                               aTCTCGTTGCTTACGCAACCAAATGTCTAATGTATGTCACTCTGCATCAATGGCACGATATAAATAGCTCCGTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCCGTGGTAATAAGCTTTTTTTTGGTTTttgtt                                              >  2:322899/1‑141 (MQ=11)

                                                                                               aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              <  2:678103/141‑1 (MQ=255)

                                                                                               aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGCTATGAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              >  1:297719/1‑141 (MQ=255)

                                                                                               aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCATCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              >  1:339164/1‑141 (MQ=255)

                                                                                                tctcGTTGCTTACTCAACCACATATCTCATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                             <  1:570387/141‑1 (MQ=255)

                                                                                                tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                             <  2:207573/141‑1 (MQ=255)

                                                                                                tctcCTTGTTTACGTAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                             <  1:742063/141‑1 (MQ=255)

                                                                                                 ctcCTTGTTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACCATATAAATAGCTCCTTTTTCCTTTTTTTTTGATATACGTCTCATCAATACGCCATTTGTAATAATCTTTTTTATGCTTTTTCTTcc                                            <  2:705974/141‑1 (MQ=14)

                                                                                                  tcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTATAATAAGCTTTTTTATGCTTTTTCTTCCa                                           >  1:619506/1‑141 (MQ=255)

                                                                                                     ttGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATTAATACGCCATTTGTCATAAGCTTTTTTATGTTTTTTCTTTCAAAt                                        >  1:406045/1‑141 (MQ=14)

                                                                                                           actcAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATa                                  <  1:343269/138‑1 (MQ=255)

                                                                                                            cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGATCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGGAATAAGCTTTTTTATGCTGTTTCTTCCAAATTTGATat                                 <  1:431904/141‑2 (MQ=14)

                                                                                                            cGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTTTAATAAGCATTTTTATCCTTTTTTTTCCAAATTTGATaa                                 >  1:665849/1‑140 (MQ=255)

                                                                                                              cAACTAAATATTTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATataa                               <  1:11415/141‑4 (MQ=255)

                                                                                                              cAACCAAATATCTAATGTATGTCCCTCTGTGTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATATGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGATatat                               >  2:470008/1‑138 (MQ=14)

                                                                                                              cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATataa                               <  1:146866/141‑4 (MQ=255)

                                                                                                              aaaCCAAATATCGAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATataa                               <  1:8030/140‑4 (MQ=255)

                                                                                                                aCCAAACATCTTATTTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaaa                             <  1:328253/141‑1 (MQ=11)

                                                                                                                  cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATTTAAAAtt                           >  2:128174/1‑141 (MQ=255)

                                                                                                                    aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                         <  2:761124/141‑1 (MQ=255)

                                                                                                                        tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATCCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                     <  2:424214/141‑1 (MQ=255)

                                                                                                                         ttAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTGCTTATTTTATGATATACGCCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGTGCat                    <  1:527389/140‑1 (MQ=11)

                                                                                                                             tgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTc                <  2:342358/141‑1 (MQ=25)

                                                                                                                              ttatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATAACTATATAAAATTGGGGCATATTCt               <  2:747494/140‑1 (MQ=11)

                                                                                                                               tatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTTTTATCATTACGCCATTTTTAATAAGCTTTTTTATGCTTTTTCTTACCGTTTTGATGTAAAATTGGAGCATTTTCtc              >  1:387036/1‑140 (MQ=11)

                                                                                                                               tatgtCCCGCAGCATCAATGGCACGATATACATAGCTCCATGTTCCTTTTATCTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTCCAAAGGGGATACAAAATTGGGGCATATTCtt              <  1:183435/141‑1 (MQ=14)

                                                                                                                                atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg             <  1:479277/141‑1 (MQ=255)

                                                                                                                                  gtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa           <  1:695399/141‑1 (MQ=255)

                                                                                                                                  gtCCCTCTGTATCAAGGGCACTATATTAACTGTTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa           <  2:434632/141‑1 (MQ=11)

                                                                                                                                   tCCCTCTGCATCAATGGAACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTACTAAGCTTTTTTATGCTTTTTCTTCAAATTTTGATATAAAATTGGGGCATATTATTGAAc          >  2:618091/1‑141 (MQ=11)

                                                                                                                                       tctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCaa      >  1:564587/1‑141 (MQ=25)

                                                                                                                                        ctGTATTAATGGCACGATATAAATAGCTCCAGTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc     <  2:598961/141‑1 (MQ=11)

                                                                                                                                          gCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGTCATATTCTTGAACCCAACgg   >  1:123794/1‑141 (MQ=255)

GCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36130‑36409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`