BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,378	C→T	85.3%	L58L (TTG→TTA)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,378	0	C	T	85.3%	966.3 / 165.5	584	L58L (TTG→TTA)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (41/46); new base T (230/258); total (277/307)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 586 total aligned reads displayed.

CGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACA  >  CP000730/36238‑36516

    aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaaa                                                                                                                                        >  2:587379/1‑141 (MQ=38)

        aaTATCTAATGTATCTCCCTCAGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCACTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTgg                                                                                                                                    >  2:529904/1‑141 (MQ=11)

          tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTTATAAGCTTTTTTATGCTTTTTCTTTCGAATTTGATATAAAATTcggg                                                                                                                                  >  2:282374/1‑141 (MQ=11)

          tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTCTATAAAATTgacc                                                                                                                                  >  2:378767/1‑138 (MQ=11)

           atCTAATGTTTGTCCCTCTGGATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGc                                                                                                                                 <  2:89789/141‑1 (MQ=255)

             ttAATGTATGTCCCTCGGTATCAATGGAACGATATAAAGAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                               <  2:660032/140‑1 (MQ=11)

             ctaattgttgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACACCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                               <  2:542034/133‑1 (MQ=11)

             cTAATTTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                               <  2:759358/141‑1 (MQ=255)

                    atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                                                                                        <  1:479277/141‑1 (MQ=255)

                    atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAGACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCtta                                                                                                                        >  2:123396/1‑140 (MQ=255)

                        cccTCTGTATCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACTTTTCCTCAATTCGCCATTTTTAATAATCTTTTTTTGGCTTTTTTTTCCACTTTTGATATAAAATTGGGGCATATTATTGAAcc                                                                                                                    >  1:84544/1‑141 (MQ=11)

                        cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCTTATTCTTGAAcc                                                                                                                    >  1:699396/1‑141 (MQ=21)

                         ccTTTGTATCATTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGGCTCCTCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAGATTTGATATAAAATTGGGGCATATTCTTGAAccc                                                                                                                   <  2:508419/141‑1 (MQ=11)

                         ccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATAAATACGCCATTTGTAATAAGCTTTTTTTTGCTTTTTCTTCCAAACTTGATACAAAATTGTGGCATATATTTGCAccc                                                                                                                   >  2:435281/1‑141 (MQ=14)

                         ccTCTGCAGCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAccc                                                                                                                   <  1:111835/141‑1 (MQ=18)

                           tctGTATCAATGGCGCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATTCGCCCTTTGTAATACGCTTTTTTACCGCTTGTCTTCCAAAATCGATATAAAATTGGGGCATATTCATGAACCCaa                                                                                                                 >  1:404376/1‑141 (MQ=11)

                           tctGCATCATGGGCACGATATAAATAGCTCCAATTTGCCGCCATGTTGATCCTCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCaa                                                                                                                 >  1:566561/1‑141 (MQ=11)

                             tgtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTACTAAACTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACg                                                                                                               >  1:353100/4‑141 (MQ=11)

                              ttaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                                                                              <  2:470077/139‑1 (MQ=255)

                              gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                                                                              <  1:662714/139‑1 (MQ=16)

                               taTCAATTGCACCATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt                                                                                                             <  2:602676/140‑1 (MQ=255)

                               taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTTATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAACTTGATATAAAATTGGGGCCTAGTCTTGAACCCAACGGt                                                                                                             <  1:431636/140‑1 (MQ=11)

                               cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCTTAATTTGATATAAAATTGGGGCATATTCTTGAATCCAACGGt                                                                                                             >  2:702612/1‑141 (MQ=255)

                                   aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGAc                                                                                                         <  2:424089/141‑1 (MQ=39)

                                    aTGGCACGATATAAATAGCTCCATTTTCGTTTTATTTTGATGTACGTCTCATCAGTACGCCATTTGTAATAAGCTTTTTCATGCTTTTTCTTCCAAATTTGATAGAAAATTGGGGCATTGTTTTGAACCCACCGGTTGAcc                                                                                                        >  1:499895/1‑141 (MQ=11)

                                    aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGACTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCATATTTGATATAAAATTGGGGCATATTCTTGAACCCAACTGTAGAcc                                                                                                        >  2:493325/1‑141 (MQ=255)

                                    aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGatc                                                                                                        <  1:624134/141‑3 (MQ=19)

                                    aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGGCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACAATTGGGGCATATTCTTGATCCCAACGGTAgttc                                                                                                        <  2:217450/141‑4 (MQ=11)

                                     tGGCACGATATAAATGGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCg                                                                                                       <  1:36772/141‑1 (MQ=21)

                                       gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAAATCGtt                                                                                                     >  2:133190/1‑141 (MQ=255)

                                         aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                   <  2:453195/141‑1 (MQ=21)

                                         aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                   >  1:2072/1‑141 (MQ=255)

                                         aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                   >  1:46445/1‑141 (MQ=255)

                                           gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTGTTGAACCCAACGGTAGATCGTTGAat                                                                                                 >  1:98721/1‑141 (MQ=255)

                                           gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCACCGGTAGATCGTTGAat                                                                                                 >  1:103599/1‑141 (MQ=255)

                                            atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCCACGGTAGATCGTTGAatg                                                                                                >  2:517524/1‑141 (MQ=255)

                                            atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGActg                                                                                                >  2:462260/1‑141 (MQ=255)

                                            atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                <  2:428992/141‑1 (MQ=255)

                                             tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATTGGGGCAGATTCTTGAACCCAACGGTAGTTCAGTGAatga                                                                                               >  1:313229/1‑141 (MQ=11)

                                               aaaaTAGCCCCATTTTCCTGTTATTTTGATGTACGTCTCATCAATAAGACATTTGTAGTAAGTTTTATGATGCGAGTTCTTCCAAATTTGATAGAAAATTGGGGCATAGTCTTGACCCCAACGGTAGACCGTTGCatggta                                                                                             <  1:733295/140‑4 (MQ=11)

                                                 aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGACCCCAACGGTAGATCGTTGAATGATGaa                                                                                           >  2:170740/1‑141 (MQ=255)

                                                  aTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGAACGTTGAATGATGAAc                                                                                          >  2:501079/1‑141 (MQ=21)

                                                  aTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAc                                                                                          >  1:553678/1‑141 (MQ=255)

                                                  aTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTCTTATGCTTTTTCTTCCAAATTTGATATAAAATTGCGGCCTATTCTTCCACCCAACGGTAGATCGTCGAATGATGAAc                                                                                          <  2:183898/141‑1 (MQ=11)

                                                    aGCTCCGTTTTCCTGTTATTTTGATATACGGCTCATCAGTACGCCGTTGGTAATAAGCTTTTGTATGCTTTTTCTTCCAAATTTGATATAGAATTGGGGCATATTCTTGAAGCCAACGGTAGATCGTTGAATGATGAACGt                                                                                        >  2:685072/1‑141 (MQ=11)

                                                    aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                                                                                        <  1:667787/141‑1 (MQ=255)

                                                    aGCGCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGt                                                                                        <  2:143563/141‑1 (MQ=255)

                                                       tCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTa                                                                                     >  2:251558/1‑141 (MQ=25)

                                                        ccATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTTGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAAGGTTTac                                                                                    >  2:332351/1‑141 (MQ=255)

                                                          aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                  <  2:260603/141‑1 (MQ=25)

                                                           ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                 <  1:516222/141‑1 (MQ=18)

                                                           ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                 <  1:328882/141‑1 (MQ=255)

                                                            tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                                <  2:69368/141‑1 (MQ=18)

                                                             ttCCTTTTATTTTGATATACGTCTCATCAATACGCCAGTTGTAATAAGCTTTTTTAAGCTTTTTGTTCCAACTTTGTTATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                               <  2:301461/141‑1 (MQ=11)

                                                                 ttttATTTTGATGTAGGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGGACGTTTACACCACGTTc                                                                           >  1:576377/1‑141 (MQ=255)

                                                                   ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTGAGGTGTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                         <  1:176829/141‑1 (MQ=11)

                                                                    tATTTTGATATACGTCTCACCAATACGCCATTTGTAATAAGCTTTTCTAGGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTTAATGATGAACGTTTACACCACGTTctct                                                                        <  2:80404/141‑4 (MQ=11)

                                                                     tttttGATGTACGTCTCATCAAGACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                       <  1:163465/140‑1 (MQ=16)

                                                                     aatttGATGGACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                       <  2:340933/139‑1 (MQ=255)

                                                                     aTTTTGATGTACCCTTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCATATTTGATTTAAATTTGGGTCATAGTCATGATCCCAACGGTAGCCCGTTGAATGATGAACGTTTACACCACGTACCCtt                                                                       <  2:162996/141‑1 (MQ=11)

                                                                     aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                       <  1:129741/141‑1 (MQ=11)

                                                                     aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                       <  1:393317/141‑1 (MQ=255)

                                                                     aTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGCAGATCGTTGAATGATGAACGATTACACCCCGTTCTCtt                                                                       >  2:153682/1‑141 (MQ=11)

                                                                      ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTa                                                                      <  1:64952/141‑1 (MQ=39)

                                                                       tttGATGTACCTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                     <  1:426309/141‑1 (MQ=255)

                                                                        ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAat                                                                    <  1:742005/141‑1 (MQ=255)

                                                                         tGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAata                                                                   >  2:620080/1‑141 (MQ=40)

                                                                          gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                  >  1:683852/1‑141 (MQ=25)

                                                                          gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATATGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                  >  1:712553/1‑141 (MQ=21)

                                                                          gATATACGTCTCAGCAATACGCCGTGTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGTTCTCTTAatat                                                                  >  2:410197/1‑141 (MQ=11)

                                                                            tatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGATTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACGCCCCGTTCTCTTGATAttt                                                                >  1:188999/3‑141 (MQ=11)

                                                                              tgcttctcATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGTACCCAACGGTAGTCCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                              <  2:645667/137‑1 (MQ=255)

                                                                               aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                             <  2:672724/141‑1 (MQ=255)

                                                                                cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGa                                                            >  1:290231/1‑141 (MQ=255)

                                                                                cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGa                                                            >  1:534428/1‑141 (MQ=21)

                                                                                cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGa                                                            >  1:591762/1‑141 (MQ=40)

                                                                                 gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGat                                                           <  1:249439/141‑1 (MQ=25)

                                                                                     catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAAGTTTACATAACTTTCTCTTAATCTTTCAGatatat                                                       >  1:756581/1‑141 (MQ=11)

                                                                                      atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                      <  1:3208/141‑1 (MQ=255)

                                                                                       tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATcc                                                     >  2:764756/1‑140 (MQ=255)

                                                                                         aaaaagatatatGTAATAAGATTTTTTATGCCATTTCTTTCTTATTTGATTCTATATTTGGGTATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACg                                                   <  2:729962/130‑1 (MQ=14)

                                                                                          aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGTTGAGCGTTTACACCACGTTATCTTAATACCTCAGATATATCACGa                                                  <  1:386207/141‑1 (MQ=11)

                                                                                          aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGa                                                  <  2:56220/141‑1 (MQ=255)

                                                                                           tACGCCATTTGTAATAAGATTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATTGGGGCATATTCTTCAACCCTACGTACGATCTTTGAATGATGAAGTTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                 >  2:365307/1‑141 (MQ=11)

                                                                                            aCGGCATTTGGAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATa                                                <  1:277935/141‑1 (MQ=255)

                                                                                            aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                <  1:258358/141‑1 (MQ=255)

                                                                                            aCGCCATTTGTAATAAGCTTCTCTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATa                                                <  1:702612/141‑1 (MQ=12)

                                                                                                cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTc                                            <  1:413870/141‑1 (MQ=255)

                                                                                                cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                            <  1:608841/141‑1 (MQ=21)

                                                                                                cATTGGTAATAAGCTGTTTTAGGCTTTTGCTTCCGAATTTGATATAAAGGTGGTTCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTTAGATATATCACGATAGCTc                                            <  1:144938/141‑1 (MQ=11)

                                                                                                 aTTTGTAATAAGCTTTTTTTTTTTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTGGATGCATGAACCCCAACACCACGTTCTCTTAATATTTCAGATATATctctttagctca                                           >  2:743153/1‑130 (MQ=11)

                                                                                                   ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGTTATATCACGATAGCTCAAt                                         <  1:239669/141‑1 (MQ=11)

                                                                                                   ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                         <  1:491019/141‑1 (MQ=255)

                                                                                                   ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                         >  2:388667/1‑141 (MQ=40)

                                                                                                    tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGTACTTTGACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                        <  1:448306/141‑1 (MQ=11)

                                                                                                    tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAGAGCTCactt                                        >  2:711581/1‑138 (MQ=11)

                                                                                                     gTAATAAGCTTTGTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgc                                       >  2:241184/1‑140 (MQ=17)

                                                                                                      taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACCCAATgca                                      <  1:219323/141‑3 (MQ=12)

                                                                                                        ataaGCTTTTTTATGCTTTTTTTTTTTTATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGGCCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGtttt                                    >  2:350404/1‑138 (MQ=14)

                                                                                                                                         aTACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAc   >  2:205029/1‑141 (MQ=35)

CGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACA  >  CP000730/36238‑36516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`