BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,413	C→T	54.8%	V47I (GTC→ATC)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,413	0	C	T	54.8%	39.4 / inf	418	V47I (GTC→ATC)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (81/104); new base T (105/120); total (191/227)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.82e-01

Only 100 of 425 total aligned reads displayed.

AATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAC  >  CP000730/36273‑36553

aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAGTACGCCATTTGTAATACGCTTTTTTAGTCTTTTTCTTCCAAGTATGATATAAAATTGTGGCATATTCTTGATCCCAACGGTAGAc                                                                                                                                              >  2:466488/1‑141 (MQ=11)

 aTGGCACGATATAAATAGCTCCATTTTCGTTTTATTTTGATGTACGTCTCATCAGTACGCCATTTGTAATAAGCTTTTTCATGCTTTTTCTTCCAAATTTGATAGAAAATTGGGGCATTGTTTTGAACCCACCGGTTGAcc                                                                                                                                             >  1:499895/1‑141 (MQ=11)

  tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCg                                                                                                                                            <  2:440431/141‑1 (MQ=40)

   ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                                                           <  2:492310/141‑1 (MQ=255)

      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCGAATTTGAAAAAAAATTTGGGTATCTCCTTGAACCCAAGGGTAGTACGttaa                                                                                                                                        >  2:313507/1‑139 (MQ=11)

      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                                                        >  2:606111/1‑141 (MQ=255)

       cGATATAAATTGCTCCATTTTCCTTTGATTTTGATATACGTCTTATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                                                       <  2:713851/141‑1 (MQ=11)

        gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCACCGGTAGATCGTTGAat                                                                                                                                      >  1:103599/1‑141 (MQ=255)

         atatAAATATCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                                                     <  2:497936/141‑1 (MQ=255)

         atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCCACGGTAGATCGTTGAatg                                                                                                                                     >  2:517524/1‑141 (MQ=255)

         atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                                                     <  2:334208/141‑1 (MQ=255)

          tataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatga                                                                                                                                    <  2:170993/141‑1 (MQ=18)

           ataAATAGCTCCATTTTCTTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGCAGATCGTTGAatgat                                                                                                                                   <  1:389068/141‑1 (MQ=11)

           ataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgat                                                                                                                                   >  1:340620/1‑141 (MQ=255)

            taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATTTAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCTTTTAatgttg                                                                                                                                  >  2:341502/1‑141 (MQ=11)

             aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTGGATCGGTGAAtgatga                                                                                                                                 >  1:289581/1‑141 (MQ=11)

                tAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACg                                                                                                                              <  1:621209/141‑1 (MQ=255)

                 aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAAAGCGTTTAAGGGTGAACGt                                                                                                                             >  2:729662/1‑141 (MQ=11)

                    tCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTa                                                                                                                          >  2:251558/1‑141 (MQ=25)

                     ccATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTCTGCTTTTTCTTCCAAGTTTGATATCTCATTTGGAGATATTCTTGAACCCAACGGTAGACCGTTAAATGATGAACGTTTac                                                                                                                         >  2:430/1‑141 (MQ=11)

                     ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAAATCGTTGAATGATGAACGTTTac                                                                                                                         >  2:14432/1‑141 (MQ=255)

                      cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGCCCGTTGAATGATGAACGTTTaac                                                                                                                        >  2:20563/1‑139 (MQ=19)

                      cATTTTCCTTTTATTTTGATATACGTCTCATCGAACAGCGCGGTGGAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                                                        <  1:210870/141‑1 (MQ=11)

                       aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                                                       >  2:628696/1‑141 (MQ=21)

                       aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                                                       <  2:260603/141‑1 (MQ=25)

                       aTTTTCCTTTAATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTCGATCGTTGAATGATGCACGTTTacgc                                                                                                                       >  2:522799/1‑139 (MQ=11)

                        ttttCCTTTTATTTTGATATGCGTCTCAGCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                                                      <  1:660178/141‑1 (MQ=11)

                         tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                                                                     <  1:376575/141‑1 (MQ=21)

                         tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                                                                     <  2:736075/141‑1 (MQ=21)

                         tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                                                                                                                     <  2:18233/141‑1 (MQ=255)

                         tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                                                                                                                     <  1:680780/141‑1 (MQ=255)

                          ttCCTTTTATTTTGATTGTGGTTTCATCAATACGCCATTTGTAAGAAGCTTTTTTATGCTTTTTCTTCCAAATTTGACATAAAATTGTGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                                                                    <  1:724397/141‑1 (MQ=11)

                          ttCCTTTTATTTTGATGTACGTCTCATCAATACTCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGTCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                                                                                                                    <  2:703949/141‑1 (MQ=255)

                          ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGATTTTTTATGCTTTTTCTTCCAAATATGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTCctccac                                                                                                                    >  1:227665/1‑141 (MQ=11)

                          ttCCTTGTATTGTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                                                                    <  2:539204/141‑1 (MQ=11)

                           tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTTTAATAATCTTTTTTATGCTTTTGCTTCCAAATTTGATATAAAATTTGGGCATATTCTTGAACCCATCGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                                                                   <  2:154048/141‑1 (MQ=11)

                            ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                                                                  <  1:458992/141‑1 (MQ=21)

                            ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                                                                  <  1:300935/141‑1 (MQ=21)

                            ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                                                       <  2:588264/136‑1 (MQ=40)

                             cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                                                                 >  2:118764/1‑141 (MQ=255)

                             cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                                                                 <  2:668174/141‑1 (MQ=255)

                              ttttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTc                                                                                                                >  1:358346/1‑141 (MQ=21)

                              ttttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTc                                                                                                                >  2:200717/1‑141 (MQ=255)

                                 tgtttaGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                                                             <  2:380015/139‑1 (MQ=19)

                                  tttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                                                            <  2:457295/140‑1 (MQ=21)

                                  aTTTTGATGTACCCTTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCATATTTGATTTAAATTTGGGTCATAGTCATGATCCCAACGGTAGCCCGTTGAATGATGAACGTTTACACCACGTACCCtt                                                                                                            <  2:162996/141‑1 (MQ=11)

                                    tttGATGTACCTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                                                          <  1:426309/141‑1 (MQ=255)

                                    tttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                                                          <  1:713741/141‑1 (MQ=11)

                                     tttaTGTACGTCTCATCAATTCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAat                                                                                                         <  1:399320/138‑1 (MQ=255)

                                     ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGTTCTCTTAat                                                                                                         >  1:164061/1‑141 (MQ=11)

                                       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGTTCTCTTAatat                                                                                                       >  1:107409/5‑141 (MQ=11)

                                       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTGTTTCTTCCAAATTTGGTATAAAATTGGAGCATAATCTTGAACGCAACGATCGATCGTTGAATGATGAACGGTTACACCACGTTCTCTTActat                                                                                                       >  2:506007/5‑141 (MQ=11)

                                       gATGTACGTCTCATCAATATGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                                                       >  2:654021/1‑141 (MQ=25)

                                       gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                                                       >  1:134130/1‑141 (MQ=25)

                                       gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                                                       >  1:147362/1‑141 (MQ=21)

                                       gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGGTCCCTTAatat                                                                                                       >  2:694047/1‑141 (MQ=255)

                                       gATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAatat                                                                                                       >  2:772486/1‑141 (MQ=255)

                                        atatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTCTGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAtt                                                                                                      >  1:530042/4‑141 (MQ=11)

                                         tatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAttt                                                                                                     >  1:123854/3‑141 (MQ=255)

                                           tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                                                                   <  2:107110/141‑1 (MQ=255)

                                            aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAg                                                                                                  <  1:325249/141‑1 (MQ=255)

                                            aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAg                                                                                                  >  2:137139/1‑141 (MQ=21)

                                             cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCTCGTTCCCTTAATCTTTCAGa                                                                                                 <  1:484139/141‑1 (MQ=255)

                                              gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGat                                                                                                <  1:249439/141‑1 (MQ=25)

                                               tctcATCAATACGCCAATTGTAATATGCTGTCTTATGCTTTTTATTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACACAAAGGTAGACCAATGAATGATGAACGTTTACACCCCGTTCTCTTAATATTTCAGata                                                                                               >  2:281650/1‑141 (MQ=11)

                                                  catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                                                            <  1:73912/141‑1 (MQ=255)

                                                  catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatatat                                                                                            >  2:749604/1‑141 (MQ=18)

                                                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                                                           >  1:18752/1‑141 (MQ=21)

                                                    tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACATTTAAGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                                                          <  1:562827/141‑1 (MQ=11)

                                                     caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTTTTATTTTTATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAc                                                                                         >  2:687007/1‑141 (MQ=14)

                                                      aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACg                                                                                        <  2:227562/141‑1 (MQ=255)

                                                        tACGCCATTTGTAATAAGATTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATTGGGGCATATTCTTCAACCCTACGTACGATCTTTGAATGATGAAGTTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                                                      >  2:365307/1‑141 (MQ=11)

                                                         aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                                                     <  1:429601/141‑1 (MQ=255)

                                                         aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATa                                                                                     <  1:753284/141‑1 (MQ=21)

                                                         aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATa                                                                                     <  2:761582/141‑1 (MQ=39)

                                                           gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                                                   <  1:87834/141‑3 (MQ=255)

                                                           gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                                                   >  2:405368/1‑141 (MQ=40)

                                                             cATTTGTAATATGCTTTTTTATGCCTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                 <  1:589543/141‑1 (MQ=255)

                                                             cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATTAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                 <  2:85226/141‑1 (MQ=25)

                                                             cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTc                                                                                 <  1:608841/141‑1 (MQ=21)

                                                             cATTTGTAATAAGCTATTTTATGCTTTTTCTTCCAAAATTGATCCAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAATGTTTACACCACGTTACCTTAATATTTCAGATATCTCACGATGACTc                                                                                 <  1:545325/141‑1 (MQ=11)

                                                                ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAGTTTGATATAAAATTGGGGCCTGTTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                                              <  2:424159/141‑1 (MQ=11)

                                                                ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGCTATATCACGATAGCTCAAt                                                                              <  1:411916/141‑1 (MQ=11)

                                                                ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                                                              >  2:388667/1‑141 (MQ=40)

                                                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTTATATTTCAGATATATCACGATAGCTCAATg                                                                             <  1:202443/141‑1 (MQ=11)

                                                                  gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGt                                                                            >  2:241952/1‑141 (MQ=255)

                                                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTTCACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                           >  1:440904/1‑139 (MQ=12)

                                                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                           <  1:630798/141‑3 (MQ=17)

                                                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGGTGACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATgca                                                                           <  2:566561/141‑3 (MQ=255)

                                                                   taataaGCTTTTTTATGCTTTTTCCTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGta                                                                           <  1:31446/141‑1 (MQ=255)

                                                                        aGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCt                                                                      >  1:700288/1‑141 (MQ=38)

                                                                                      ttttCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAGTATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCaa                                                        <  1:512114/141‑1 (MQ=25)

                                                                                           ttcCAAATTTGATACAAAATTGGGGCATATTCTGGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGcc                                                   >  1:263856/1‑140 (MQ=25)

                                                                                             ccAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTAc                                                 >  1:706936/1‑141 (MQ=35)

                                                                                                   ttGATACAAAATTGGGGCATATTCTGTAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATACTAGCCAACGGCTACAGTGAt                                           <  1:469523/141‑1 (MQ=255)

                                                                                                      aTACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATTAACGTTTACACACCGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGGCAACGGCTACGGTGATaaa                                        >  1:24037/1‑140 (MQ=11)

                                                                                                                   gCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTACCCTTAATATTTCAGATATATCACGAAAACTCAATGTATATCTAAAATAGTAGCCAACGGCTACAGTGATAACATCCTGGTTAAAt                           >  2:149335/1‑141 (MQ=11)

                                                                                                                                  cAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGaaa            <  2:675258/141‑1 (MQ=32)

                                                                                                                                      ggTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATCACATCCTTGTTAAATTGTTTATATCTGAAATAGt        >  1:66696/1‑141 (MQ=17)

                                                                                                                                         aGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACCGTGATAACATCCTTGTTGAATTGTTTATCGCTGAAATAGTTCa     >  1:281306/1‑141 (MQ=255)

AATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATAC  >  CP000730/36273‑36553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`