Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,180 C→T 36.7% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1800CT36.7% 140.8 / 83.5 95intergenic (+238/‑896)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (29/31);  new base T (15/20);  total (44/51)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

ATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTA  >  CP000730/76048‑76319
                                                                                                                                    |                                                                                                                                           
atatAACTAATACTGCTGCACCTGTATCACTTAGAACAGCTAACCTAAGTGTTAATAATCGAGGGTCTACTAATACAACGGGCATTAATTTAATAATTATAGCAACCTATATGTTATGTTGAATAGTTGAATCTTTAGtag                                                                                                                                     <  2:323024/141‑2 (MQ=255)
      cTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAtttt                                                                                                                               <  2:636275/141‑1 (MQ=255)
       aaaTAGTGCTGACCCTGTACCACGTAAAACGGGTAACCAACGTGTTAGTAATCCAGGGACTACTATCAAAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTa                                                                                                                              <  2:120544/140‑1 (MQ=37)
        aaTATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTaa                                                                                                                             >  1:350826/1‑141 (MQ=255)
             tgctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTGAACaaaa                                                                                                                        >  2:73002/1‑141 (MQ=255)
              gctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGAAATTAATTTAATAATTATAGAAAAATATAGGTACTGTTGCAAAGTTGAATCTATAGTATAATTTTAGCaaaaa                                                                                                                       >  2:107260/1‑141 (MQ=255)
               ctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGGTAGTAGTCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaaaaaa                                                                                                                      >  2:386505/1‑140 (MQ=255)
                  cACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATGGTATAATTTTAACAAAAAGGAg                                                                                                                   >  1:303972/1‑141 (MQ=255)
                      tGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAAGATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtctt                                                                                                               <  1:377489/141‑1 (MQ=255)
                       gTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtcttc                                                                                                              <  2:580315/141‑1 (MQ=255)
                           cACTTAGAACAGCTAACCAAAGTGTTAGTAAGCCAGGTAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAATCTTCTGtt                                                                                                          >  2:28266/1‑140 (MQ=255)
                             cTTAAAACAGCTAACCAAAGTGTTAGAAATCCAGGGAATACTAACACAAAGGCAATTCATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATg                                                                                                        <  1:444782/141‑1 (MQ=255)
                                aGAACATCTAACCAAACTCTTAGTAATCCATGGACTACTAAAAAAAAGGCAATTAATTTAAACATTATAGCAAAATATATGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAAc                                                                                                     <  1:205813/141‑1 (MQ=21)
                                 gAACAGCGAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACt                                                                                                    <  1:356323/141‑1 (MQ=255)
                                   aCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAt                                                                                                  >  1:148005/1‑141 (MQ=255)
                                   aCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAt                                                                                                  >  2:171322/1‑141 (MQ=255)
                                      gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTc                                                                                               >  1:456569/1‑141 (MQ=255)
                                       cTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCa                                                                                              >  1:512578/1‑141 (MQ=255)
                                         aaCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGa                                                                                            <  1:694611/141‑1 (MQ=255)
                                             aaaGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGAtata                                                                                        >  2:289285/1‑141 (MQ=255)
                                              aaGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATCGCAAAATCAAGGGTCTTTTGCATAGTTGCATCTATAGTATAATTTTAACAAAAATGAGTCCTCTGTATGAACTATTTCAGATaaaa                                                                                       >  2:314075/1‑141 (MQ=25)
                                              aaGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAAGCTAGAGTATAATTATAACAAAAATGAGTCTTCTGTATGAACCATTTCAGATCTaa                                                                                       >  1:331744/1‑141 (MQ=37)
                                                   ttAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAt                                                                                  >  2:539071/1‑141 (MQ=255)
                                                     aGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                                <  1:399573/141‑1 (MQ=255)
                                                     aGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                                <  1:77077/141‑1 (MQ=255)
                                                     aGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAAAAAttt                                                                                >  2:155880/1‑141 (MQ=255)
                                                       tAATCTTGGGAATACTAACACAAAGGCAAGTAATTTAATAATTATAGCAAAATATAGGATCTGTTGCAAAGTTGAATCTATAGTATAATAATAACAAAAAGGAGTCTTCTGTATGAACTGTTTCAGATATAAACAATTTaa                                                                              <  2:531253/141‑1 (MQ=21)
                                                        aaTCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTGAACAAAAAGGAGGCTTCTGTATGAACTATTTCAGATATAAACAATTTAAc                                                                             >  1:376412/1‑141 (MQ=255)
                                                         aTCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACa                                                                            <  2:592253/141‑1 (MQ=255)
                                                         aTCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACa                                                                            <  2:662009/141‑1 (MQ=255)
                                                                 aaTACTAACAAAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGtt                                                                    <  1:45071/141‑1 (MQ=255)
                                                                   tACTAACACAAAGGCAATTCATTTAATAATTATAGCAAAATATAAGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAAAAATTTAACAAGAATGTTAt                                                                  >  1:423798/1‑141 (MQ=255)
                                                                   aaCTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTAt                                                                  <  2:749540/140‑1 (MQ=255)
                                                                        acacAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTg                                                             <  1:417931/141‑1 (MQ=255)
                                                                        acacAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTg                                                             <  1:219040/141‑1 (MQ=255)
                                                                           caAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAg                                                          <  1:32798/141‑1 (MQ=255)
                                                                           aaaaGGGAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAg                                                          <  2:622819/140‑1 (MQ=255)
                                                                             aaGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGACCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                        <  2:581603/141‑1 (MQ=255)
                                                                             aaGGCAATTAATTTAATAATTATAACAAAATATAGGGTCTGTTGCAAAGTTGAATCTATACTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAAAAAGGTTGTTATCACTGTAGcc                                                        <  1:397733/141‑1 (MQ=18)
                                                                              aGGAAATTAATTTAATAATTATAGCAAAATATCGGTTCTGTTGCAAAGTCGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCg                                                       <  1:494402/141‑1 (MQ=255)
                                                                                 cAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTg                                                    >  2:171482/1‑141 (MQ=255)
                                                                                 cAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAGTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATTTAAACAATTTAACAAGGATGTTATCACTGTAGCCGtgg                                                    >  1:436061/1‑139 (MQ=255)
                                                                                    ttaatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTAATCACTGTAGCCGTTGGct                                                 <  1:409410/141‑1 (MQ=255)
                                                                                       atttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctact                                              <  2:560710/141‑1 (MQ=255)
                                                                                                     gCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCa                                >  2:21522/1‑141 (MQ=255)
                                                                                                      cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCATTGGCTACTATCTAAGATATGCAt                               >  2:220968/9‑141 (MQ=1)
                                                                                                      cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCATTGGCTACTATCTAAGATATGCAt                               >  2:221185/9‑141 (MQ=11)
                                                                                                      cagttaggaGGTTCTGTTGCAAAGTTGAATTTAAACTATACATTTAATAGAGAGGCGGCTGCGGTATGAACTATGGCAGGTATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTGCTCTCTAAGACATGCAt                               >  1:455759/9‑141 (MQ=2)
                                                                                                      agtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATAACTGTAGCTGTTGGCTACTATCTAAGATATGCAt                               >  2:315709/9‑141 (MQ=1)
                                                                                                       ataaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAtt                              <  1:20736/134‑1 (MQ=11)
                                                                                                        taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTGCAGATGTCACCCATTTAACAAGGCTGTTATCACTGTAGCTGTTTGCTACTATCTAAGGTATGCATTg                             >  1:366287/7‑141 (MQ=11)
                                                                                                        gttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                             >  1:510702/7‑141 (MQ=255)
                                                                                                        aaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                             <  1:190373/135‑1 (MQ=2)
                                                                                                         ttagtaGGTTCTGTTGCAAAGTTGAATTTATAATAAAATTTAAAAAAAAAGGAGTCTTCTGTACGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCGAAGATATGCATTGa                            <  2:528648/137‑1 (MQ=11)
                                                                                                         ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTAAGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCGAAGATATGCATTGa                            <  2:528519/136‑1 (MQ=11)
                                                                                                         aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                            <  1:376557/136‑1 (MQ=9)
                                                                                                         aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                            >  1:660135/6‑141 (MQ=2)
                                                                                                          taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                           >  1:606111/5‑141 (MQ=2)
                                                                                                          taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                           <  2:117160/137‑1 (MQ=9)
                                                                                                          taggaGGTTCTGGTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                           <  2:493651/137‑1 (MQ=255)
                                                                                                          taggaGGTTCGGATGCAAAGTTGAAATTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGACCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCCACTATCTAAGATATGCATTGAg                           <  2:410069/137‑1 (MQ=11)
                                                                                                          acagaGGTTCTGTTGCAAAGTTGAATTTATAGTATAAAAAAAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                           <  2:163367/137‑1 (MQ=11)
                                                                                                          aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                           <  1:405533/137‑1 (MQ=9)
                                                                                                           ttatggTTCTGTTGGAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                          <  2:188889/137‑2 (MQ=255)
                                                                                                           atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACACGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                          <  1:241910/138‑1 (MQ=2)
                                                                                                           atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                          <  1:244662/138‑1 (MQ=2)
                                                                                                           aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                          <  2:320997/138‑2 (MQ=9)
                                                                                                           aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                          >  1:164645/4‑140 (MQ=0)
                                                                                                           aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                          <  2:735623/138‑2 (MQ=2)
                                                                                                           aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                          >  2:486166/4‑140 (MQ=0)
                                                                                                           aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                          >  1:372417/4‑140 (MQ=2)
                                                                                                           aggaGGGTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                          >  2:520010/4‑140 (MQ=1)
                                                                                                           agaaGGTTCTGCTAGAAAGTTGAATTTATAATATAATTTTACCAAAAAGGAGTCTTCTGTATGAACTATCTCACGTAGTAACAATTTGACATGGATGTCATCACTGTAGCAGTTGGCTACTATCTAGGATACGCATTGAgc                          <  2:360536/138‑2 (MQ=2)
                                                                                                             taGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTa                        >  2:414780/1‑141 (MQ=37)
                                                                                                             taGGTTCTGTTGCAAAGTTGAAGCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTGAACAAGGATGTTATCCCTGTAGCCGTTGGCTACTATCTAAGATATGCATTTAGTTa                        >  2:280027/1‑141 (MQ=255)
                                                                                                             gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                        >  2:18752/2‑141 (MQ=0)
                                                                                                             gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                        <  2:81187/140‑1 (MQ=14)
                                                                                                             gaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGACATGCATTGAgcta                        <  2:90084/140‑4 (MQ=255)
                                                                                                              aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTATGATATGCATTGAGatat                       >  2:518197/1‑141 (MQ=0)
                                                                                                              aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                       >  2:187431/1‑141 (MQ=1)
                                                                                                              aGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTAt                       >  2:131624/1‑141 (MQ=35)
                                                                                                              aGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTAt                       >  2:131612/1‑141 (MQ=35)
                                                                                                              aGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTGCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTAt                       >  1:530443/1‑141 (MQ=25)
                                                                                                                   cTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATGACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGa                  <  2:496349/141‑1 (MQ=25)
                                                                                                                   cTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGa                  <  2:350826/141‑1 (MQ=35)
                                                                                                                     gTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGGATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGata                <  1:234895/141‑1 (MQ=255)
                                                                                                                     gTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGata                >  1:750073/1‑141 (MQ=35)
                                                                                                                           aaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGGTATATCTg          >  2:519915/1‑141 (MQ=21)
                                                                                                                           aaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTg          <  1:289285/141‑1 (MQ=35)
                                                                                                                            aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa         <  1:745017/141‑1 (MQ=35)
                                                                                                                            aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa         <  1:391994/141‑1 (MQ=35)
                                                                                                                            aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa         <  2:228487/141‑1 (MQ=35)
                                                                                                                              ttGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaca       >  1:453253/1‑139 (MQ=25)
                                                                                                                                 aaTTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTTTCGTGATATATCTGAAatat    <  1:471271/141‑1 (MQ=255)
                                                                                                                                   tCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATGAGCAATTTCACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTGAGATGTGCATTGAGTTATCGTGGTGTATCTGAGGTATTa  >  1:258088/1‑141 (MQ=11)
                                                                                                                                    |                                                                                                                                           
ATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTA  >  CP000730/76048‑76319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: