Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,185 G→A 19.5% intergenic (+243/‑891) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1850GA19.5% 190.5 / 37.7 106intergenic (+243/‑891)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (40/43);  new base A (8/12);  total (49/57)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

ATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGG  >  CP000730/76048‑76323
                                                                                                                                         |                                                                                                                                          
atatAACTAATACTGCTGCACCTGTATCACTTAGAACAGCTAACCTAAGTGTTAATAATCGAGGGTCTACTAATACAACGGGCATTAATTTAATAATTATAGCAACCTATATGTTATGTTGAATAGTTGAATCTTTAGtag                                                                                                                                         <  2:323024/141‑2 (MQ=255)
      cTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAtttt                                                                                                                                   <  2:636275/141‑1 (MQ=255)
       aaaTAGTGCTGACCCTGTACCACGTAAAACGGGTAACCAACGTGTTAGTAATCCAGGGACTACTATCAAAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTa                                                                                                                                  <  2:120544/140‑1 (MQ=37)
             tgctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTGAACaaaa                                                                                                                            >  2:73002/1‑141 (MQ=255)
               ctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGGTAGTAGTCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaaaaaa                                                                                                                          >  2:386505/1‑140 (MQ=255)
                  cACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATGGTATAATTTTAACAAAAAGGAg                                                                                                                       >  1:303972/1‑141 (MQ=255)
                       gTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtcttc                                                                                                                  <  2:580315/141‑1 (MQ=255)
                           cACTTAGAACAGCTAACCAAAGTGTTAGTAAGCCAGGTAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAATCTTCTGtt                                                                                                              >  2:28266/1‑140 (MQ=255)
                             cTTAAAACAGCTAACCAAAGTGTTAGAAATCCAGGGAATACTAACACAAAGGCAATTCATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATg                                                                                                            <  1:444782/141‑1 (MQ=255)
                                aGAACATCTAACCAAACTCTTAGTAATCCATGGACTACTAAAAAAAAGGCAATTAATTTAAACATTATAGCAAAATATATGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAAc                                                                                                         <  1:205813/141‑1 (MQ=21)
                                 gAACAGCGAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACt                                                                                                        <  1:356323/141‑1 (MQ=255)
                                   aCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAt                                                                                                      >  2:171322/1‑141 (MQ=255)
                                   aCAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAt                                                                                                      >  1:148005/1‑141 (MQ=255)
                                      gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTc                                                                                                   >  1:456569/1‑141 (MQ=255)
                                       cTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCa                                                                                                  >  1:512578/1‑141 (MQ=255)
                                         aaCCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGa                                                                                                <  1:694611/141‑1 (MQ=255)
                                             aaaGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGAtata                                                                                            >  2:289285/1‑141 (MQ=255)
                                              aaGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATCGCAAAATCAAGGGTCTTTTGCATAGTTGCATCTATAGTATAATTTTAACAAAAATGAGTCCTCTGTATGAACTATTTCAGATaaaa                                                                                           >  2:314075/1‑141 (MQ=25)
                                              aaGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAAGCTAGAGTATAATTATAACAAAAATGAGTCTTCTGTATGAACCATTTCAGATCTaa                                                                                           >  1:331744/1‑141 (MQ=37)
                                                   ttAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAt                                                                                      >  2:539071/1‑141 (MQ=255)
                                                     aGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                                    <  1:77077/141‑1 (MQ=255)
                                                     aGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                                    <  1:399573/141‑1 (MQ=255)
                                                       tAATCTTGGGAATACTAACACAAAGGCAAGTAATTTAATAATTATAGCAAAATATAGGATCTGTTGCAAAGTTGAATCTATAGTATAATAATAACAAAAAGGAGTCTTCTGTATGAACTGTTTCAGATATAAACAATTTaa                                                                                  <  2:531253/141‑1 (MQ=21)
                                                        aaTCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTGAACAAAAAGGAGGCTTCTGTATGAACTATTTCAGATATAAACAATTTAAc                                                                                 >  1:376412/1‑141 (MQ=255)
                                                         aTCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACa                                                                                <  2:662009/141‑1 (MQ=255)
                                                         aTCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACa                                                                                <  2:592253/141‑1 (MQ=255)
                                                                   tACTAACACAAAGGCAATTCATTTAATAATTATAGCAAAATATAAGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAAAAATTTAACAAGAATGTTAt                                                                      >  1:423798/1‑141 (MQ=255)
                                                                        acacAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTg                                                                 <  1:219040/141‑1 (MQ=255)
                                                                        acacAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTg                                                                 <  1:417931/141‑1 (MQ=255)
                                                                           caAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAg                                                              <  1:32798/141‑1 (MQ=255)
                                                                           aaaaGGGAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAg                                                              <  2:622819/140‑1 (MQ=255)
                                                                             aaGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGACCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                            <  2:581603/141‑1 (MQ=255)
                                                                             aaGGCAATTAATTTAATAATTATAACAAAATATAGGGTCTGTTGCAAAGTTGAATCTATACTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAAAAAGGTTGTTATCACTGTAGcc                                                            <  1:397733/141‑1 (MQ=18)
                                                                              aGGAAATTAATTTAATAATTATAGCAAAATATCGGTTCTGTTGCAAAGTCGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCg                                                           <  1:494402/141‑1 (MQ=255)
                                                                                 cAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTg                                                        >  2:171482/1‑141 (MQ=255)
                                                                                 cAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAGTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATTTAAACAATTTAACAAGGATGTTATCACTGTAGCCGtgg                                                        >  1:436061/1‑139 (MQ=255)
                                                                                       atttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctact                                                  <  2:560710/141‑1 (MQ=255)
                                                                                                     gCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCa                                    >  2:21522/1‑141 (MQ=255)
                                                                                                      cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCATTGGCTACTATCTAAGATATGCAt                                   >  2:220968/9‑141 (MQ=1)
                                                                                                      cagttaggaGGTTCTGTTGCAAAGTTGAATTTAAACTATACATTTAATAGAGAGGCGGCTGCGGTATGAACTATGGCAGGTATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTGCTCTCTAAGACATGCAt                                   >  1:455759/9‑141 (MQ=2)
                                                                                                        taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTGCAGATGTCACCCATTTAACAAGGCTGTTATCACTGTAGCTGTTTGCTACTATCTAAGGTATGCATTg                                 >  1:366287/7‑141 (MQ=11)
                                                                                                        aaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                                 <  1:190373/135‑1 (MQ=2)
                                                                                                         ttagtaGGTTCTGTTGCAAAGTTGAATTTATAATAAAATTTAAAAAAAAAGGAGTCTTCTGTACGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCGAAGATATGCATTGa                                <  2:528648/137‑1 (MQ=11)
                                                                                                         ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTAAGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCGAAGATATGCATTGa                                <  2:528519/136‑1 (MQ=11)
                                                                                                         aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                                >  1:660135/6‑141 (MQ=2)
                                                                                                         aaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGa                                <  1:376557/136‑1 (MQ=9)
                                                                                                          taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                               <  2:117160/137‑1 (MQ=9)
                                                                                                          taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                               >  1:606111/5‑141 (MQ=2)
                                                                                                          taggaGGTTCTGGTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                               <  2:493651/137‑1 (MQ=255)
                                                                                                          taggaGGTTCGGATGCAAAGTTGAAATTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGACCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCCACTATCTAAGATATGCATTGAg                               <  2:410069/137‑1 (MQ=11)
                                                                                                          acagaGGTTCTGTTGCAAAGTTGAATTTATAGTATAAAAAAAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                               <  2:163367/137‑1 (MQ=11)
                                                                                                          aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                               <  1:405533/137‑1 (MQ=9)
                                                                                                           ttatggTTCTGTTGGAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                              <  2:188889/137‑2 (MQ=255)
                                                                                                           atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACACGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                              <  1:241910/138‑1 (MQ=2)
                                                                                                           atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                              <  1:244662/138‑1 (MQ=2)
                                                                                                           aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                              >  1:372417/4‑140 (MQ=2)
                                                                                                           aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                              <  2:735623/138‑2 (MQ=2)
                                                                                                           aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                              <  2:320997/138‑2 (MQ=9)
                                                                                                           aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                              >  1:164645/4‑140 (MQ=0)
                                                                                                           aggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                              >  2:486166/4‑140 (MQ=0)
                                                                                                           aggaGGGTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                              >  2:520010/4‑140 (MQ=1)
                                                                                                             taGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTa                            >  2:414780/1‑141 (MQ=37)
                                                                                                             taGGTTCTGTTGCAAAGTTGAAGCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTGAACAAGGATGTTATCCCTGTAGCCGTTGGCTACTATCTAAGATATGCATTTAGTTa                            >  2:280027/1‑141 (MQ=255)
                                                                                                             gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                            <  2:81187/140‑1 (MQ=14)
                                                                                                              aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                           >  2:187431/1‑141 (MQ=1)
                                                                                                              aGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTAt                           >  2:131612/1‑141 (MQ=35)
                                                                                                              aGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTAt                           >  2:131624/1‑141 (MQ=35)
                                                                                                              aGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTGCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTAt                           >  1:530443/1‑141 (MQ=25)
                                                                                                                   cTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATGACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGa                      <  2:496349/141‑1 (MQ=25)
                                                                                                                   cTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGa                      <  2:350826/141‑1 (MQ=35)
                                                                                                                     gTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGGATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGata                    <  1:234895/141‑1 (MQ=255)
                                                                                                                     gTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGata                    >  1:750073/1‑141 (MQ=35)
                                                                                                                           aaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGGTATATCTg              >  2:519915/1‑141 (MQ=21)
                                                                                                                           aaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTg              <  1:289285/141‑1 (MQ=35)
                                                                                                                            aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa             <  1:391994/141‑1 (MQ=35)
                                                                                                                            aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa             <  2:228487/141‑1 (MQ=35)
                                                                                                                            aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa             <  1:745017/141‑1 (MQ=35)
                                                                                                                              ttGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaca           >  1:453253/1‑139 (MQ=25)
                                                                                                                               tgtatttataCTAGAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCGGTTGGCTACTATCTAAGATATGCATTGCGCTATCGTGATATATCTGAAat          <  1:743798/135‑1 (MQ=1)
                                                                                                                                 aaTTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTTTCGTGATATATCTGAAatat        <  1:471271/141‑1 (MQ=255)
                                                                                                                                  atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt       <  2:750508/138‑1 (MQ=2)
                                                                                                                                  atttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt       <  1:532070/138‑1 (MQ=2)
                                                                                                                                  atttataatataaTTTTAACAAAAACGAGAGGTCTGGATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTTTCGTGAGATATCGGAAATAtt       <  2:159156/138‑1 (MQ=1)
                                                                                                                                  atttataatataaCTTTAACAAAAAGGAGTCTGCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt       <  1:201888/138‑1 (MQ=11)
                                                                                                                                  atttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATAtt       >  2:138671/4‑141 (MQ=11)
                                                                                                                                  atttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATAtt       >  2:767417/4‑141 (MQ=1)
                                                                                                                                   tttataatataaTTTTAACAAAAAGGGGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGTTATATCTGAAATATTa      >  2:480928/3‑141 (MQ=11)
                                                                                                                                   tttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTa      >  1:712165/3‑141 (MQ=2)
                                                                                                                                   tttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTa      >  2:324780/3‑141 (MQ=2)
                                                                                                                                   tttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTa      <  1:283702/139‑1 (MQ=9)
                                                                                                                                   tCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATGAGCAATTTCACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTGAGATGTGCATTGAGTTATCGTGGTGTATCTGAGGTATTa      >  1:258088/1‑141 (MQ=11)
                                                                                                                                    ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa     <  1:712488/140‑1 (MQ=12)
                                                                                                                                    ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa     <  1:712270/140‑1 (MQ=12)
                                                                                                                                    ttataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTaa     <  1:749382/140‑1 (MQ=12)
                                                                                                                                    ttataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTaa     <  1:417191/140‑1 (MQ=17)
                                                                                                                                       taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg  <  2:409331/141‑1 (MQ=17)
                                                                                                                                       taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg  >  2:760565/1‑141 (MQ=17)
                                                                                                                                       taGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATAGTGATATATCTGTAATATTAAggg  >  2:443008/1‑141 (MQ=12)
                                                                                                                                       taGTATAATTTTAACAAAAAGGAGTCTCCTGCATGCGCCATTTCAGATATAAAGAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCCGAAATATTAAggg  <  2:349677/141‑1 (MQ=11)
                                                                                                                                       taGTATAATTTTAACAAAAAGGAGACTTCTGTATGAACTATTTCATATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTCTCTAAGATATGCATTAAGTTATCGTGATATACCTGAAACATTAAggg  >  2:196291/1‑141 (MQ=11)
                                                                                                                                         |                                                                                                                                          
ATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGG  >  CP000730/76048‑76323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: