Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,333,789	T→G	13.7%	Y64D (TAT→GAT)	hflX →	GTP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,333,789	0	T	G	13.7%	143.9 / 3.2	73	Y64D (TAT→GAT)	hflX	GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (23/36);  new base G (11/2);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 4.52e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTTAGTCGGTGTACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATT  >  CP000730/1333649‑1333926                                                                                                                                             |                                                                                                                                            tCTTAGTCGTGGTACATGCTCAGGATTATAAGCAATTTAATTGTATGTCCACAATTTTGGAATTATCATCTTTATCAGAGACTTGCCAGCTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAat                                                                                                                                             <  2:83268/141‑1 (MQ=255)   ttAGTCGGTGTACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAatat                                                                                                                                           >  1:135839/1‑141 (MQ=255)   ttAGTCGGTGTACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAatat                                                                                                                                           <  1:33680/141‑1 (MQ=255)   ttAGTCGGTGTACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAatat                                                                                                                                           <  1:187098/141‑1 (MQ=255)         ggTGTACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGt‑t                                                                                                                                   >  1:165244/1‑141 (MQ=255)          gtgtACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑Tg                                                                                                                                  >  1:305219/1‑141 (MQ=255)           tgtACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑Tgg                                                                                                                                 <  1:434041/141‑1 (MQ=255)           tgtACATGCTCAAGATGATAAGCAATTTAATTTTGAGCTTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑Tgg                                                                                                                                 <  2:47306/141‑1 (MQ=255)            gtACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑Tggt                                                                                                                                >  2:659964/1‑141 (MQ=255)                  gCTCAAGATGATAAGCAATTTAATTTTGAGTCTACCATGGAGGAATTATCATCTTTATCAGAGACCAGGATACATTAAGAGTTAGGTGTTGGTGCGCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑Tggtaaaggt                                                                                                                          <  1:370475/141‑1 (MQ=255)                  gCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑Tggtaaaggt                                                                                                                          <  1:533294/141‑1 (MQ=255)                  gCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑Tggtaaaggt                                                                                                                          >  1:456883/1‑141 (MQ=255)                           gatAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGAAAAGGTAAAATTgaa                                                                                                                 >  1:305393/1‑141 (MQ=255)                             tAAGCAATTTAATTTTTAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTgaaga                                                                                                               <  1:770555/141‑1 (MQ=255)                              aaGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATTTTTATCATAAACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTgaagaa                                                                                                              <  2:232398/141‑1 (MQ=255)                                gCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGT‑TATGG‑TGGCATTGGAAAAAATGAAGAAAt                                                                                                            >  2:389514/1‑141 (MQ=255)                                 cAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAAtt                                                                                                           >  1:621237/1‑141 (MQ=255)                                 cAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACATGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAAtt                                                                                                           >  1:466265/1‑141 (MQ=255)                                  aatttaatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCGAATGT‑CACGT‑TGGAAATGGTAAAATTGAAGAAAtta                                                                                                          >  2:229243/1‑140 (MQ=255)                                  aatttaatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGG‑TGGTAAAGGTAAAATTGAAGAAAtta                                                                                                          >  2:546965/1‑140 (MQ=255)                                     ttaatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCTAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAg                                                                                                       <  2:527954/141‑1 (MQ=255)                                      taatttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGc                                                                                                      <  2:758982/141‑1 (MQ=255)                                        atttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCAt                                                                                                    >  2:414151/1‑141 (MQ=255)                                        atttTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCCTGCTTATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCAt                                                                                                    >  1:404317/1‑141 (MQ=255)                                            tGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAGGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGCACACGTCAAATAT‑CATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTAt                                                                                                >  1:175089/1‑141 (MQ=255)                                             gAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTAtt                                                                                               <  1:511996/141‑1 (MQ=255)                                               gTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGa                                                                                             <  2:530052/141‑1 (MQ=255)                                                tCTACAATGGAGGAATTATCATCTTTATCAGAAACTTGCCAACTTGAAGTGTTGGGTCAAATAACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAg                                                                                            >  1:555298/1‑141 (MQ=255)                                                  tACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCACAGGT‑TACGT‑TACTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTAAGtt                                                                                          >  2:345305/1‑141 (MQ=255)                                                  tACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGtt                                                                                          >  1:280954/1‑141 (MQ=255)                                                   aCAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATCT‑TATGT‑TGGTAAAGGTAAAATTAAAGAAATTCAAGCATTTATTAAGtta                                                                                         >  1:29023/1‑140 (MQ=255)                                                    cAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGAT‑TGTGT‑TGTTATAGAAAAAATTGAAGAAATTCAAACATTTATTGAGttaa                                                                                        >  2:203676/1‑139 (MQ=255)                                                      aTGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCaaa                                                                                      >  1:669971/1‑141 (MQ=255)                                                       tGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAg                                                                                     <  2:581978/141‑1 (MQ=255)                                                         gaggaATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGat                                                                                   <  2:285210/141‑1 (MQ=255)                                                         gaggaATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGG‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGat                                                                                   >  2:205618/1‑141 (MQ=255)                                                           ggaATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGT‑CGCCG‑TATTATAAGAAAAAAAGAAGAAATTCAGGCATTTATTGTGTTCAAAGatat                                                                                 >  2:606006/1‑141 (MQ=255)                                                             aaTTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTg                                                                               >  1:136614/1‑141 (MQ=255)                                                              aTTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGa                                                                              <  2:61691/141‑1 (MQ=255)                                                               ttATCATCTGTATCAGAGACTTGCCAACTTGAAGGGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATTG‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGAt                                                                             <  2:602884/141‑1 (MQ=255)                                                               ttATCAGCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGAt                                                                             <  2:260097/141‑1 (MQ=255)                                                                tATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAGATGT‑CGCCG‑TAGCATTGAAAAAATAAAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATg                                                                            >  2:108240/1‑141 (MQ=255)                                                                         tATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTCCTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGtcatca                                                                   <  1:722998/141‑1 (MQ=255)                                                                            cAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACaa                                                                <  1:659964/141‑1 (MQ=255)                                                                            cAGAGACATGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGT‑CGCCG‑TAGCATAAAAAAAAAAAAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCGCaa                                                                >  2:544495/1‑141 (MQ=255)                                                                                gaCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGT‑CGCGG‑TAGCAATAGAAAAAATGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAatga                                                            >  2:596779/1‑141 (MQ=255)                                                                                gaCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGAGAT‑CACCG‑TGGCAAAGAAAAAAATGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAatga                                                            >  2:116270/1‑141 (MQ=255)                                                                                gaCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATATAGTCATCACAAatga                                                            <  1:385083/141‑1 (MQ=255)                                                                                 aCTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGT‑CGCCG‑TATCATTAGAAAAATAGAAGAAGTTGAAGGATTTGTTGAGTTCGGAGATATTGATGTAGACATAACAAatgag                                                           >  2:731143/1‑140 (MQ=255)                                                                                    tGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCTCGGTGGT‑CGCCG‑TGTCATTAGAAAAAAAAAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGGTGTAGTCATGACAAACGATGaa                                                        >  2:178339/1‑141 (MQ=255)                                                                                     gCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAAt                                                       <  2:305219/141‑1 (MQ=255)                                                                                      ccAACTTGAAGTGTTGGGTCAAATTACTTAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAACGATATTGATGTTGTCCACACAAATGTTGAAtt                                                      <  2:767794/141‑1 (MQ=255)                                                                                      ccAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAAtt                                                      >  1:316755/1‑141 (MQ=255)                                                                                            tGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGAc                                                >  1:564414/1‑141 (MQ=255)                                                                                               agggtTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGc                                             <  2:34903/138‑1 (MQ=255)                                                                                               aGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAATATATTGATGTAGTCATCACAAATGATGAATTAACGACTGc                                             <  1:56656/141‑1 (MQ=255)                                                                                               aGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGc                                             <  2:416642/141‑1 (MQ=255)                                                                                               aGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAAGGATGAATTAACGACTGc                                             >  1:356517/1‑141 (MQ=255)                                                                                                  gtTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCGGTGGTCGACGTATCATAAA‑‑AAAAATAAAAAAAATACAAGCATTTATTAAATACAAAGATATCGAGGTAATTATCACAAAAGATGGATTCAcaccaacaac                                          >  2:646036/1‑132 (MQ=255)                                                                                                     gggTCAAATTACTCAAAACAGGGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAAGTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATc                                       <  2:83327/141‑1 (MQ=255)                                                                                                            aTTGCTGAAAACGGATATCTTGTATATCTCAAAGAT‑TATGT‑GGGTAAAGGTAAAAGTGAAGAAATTCAAGCATTTATGGGGTTTAAAGATATTGATGTAGTCATCACAAAGGATGAAGTAACGACTGCACAATCCAAATCa                                <  1:157010/141‑1 (MQ=255)                                                                                                             ttACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCAc                               <  1:282724/141‑1 (MQ=255)                                                                                                              tACCCAAACCAGAGATCGTGCAGATCGCAAAAAA‑TATTT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACt                              <  1:272373/141‑1 (MQ=255)                                                                                                               aCTCAAAACAGAGATCGTGTAGATCGCGGTGGT‑TGCGG‑TGGCATTAGTAAAAATGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTa                             >  2:753295/1‑141 (MQ=255)                                                                                                                   aaaaCAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAAGCCAAATCACTAAATg                         <  1:571925/141‑1 (MQ=255)                                                                                                                    aaaCAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGa                        <  2:564414/141‑1 (MQ=255)                                                                                                                      aCAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAg                      <  2:631508/141‑1 (MQ=255)                                                                                                                         gaaaTCGTGTAGGACGCAAATAT‑TCCGC‑CTTTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCtt                   <  2:578190/138‑1 (MQ=255)                                                                                                                         ataagcgagtatctCGCAAAGAC‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCACCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCtt                   <  2:582265/128‑1 (MQ=255)                                                                                                                          agaTCGTGTAGATCGCAAATAT‑TATGT‑TGGTCCCGGTGTTATGGAAGAACTTCATTCTTTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCttt                  >  1:765074/1‑141 (MQ=255)                                                                                                                            aTCGTTTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAg                <  1:619957/141‑1 (MQ=255)                                                                                                                            aTCGTGTAGACCGCAAATAG‑TATGT‑TGGTAGAGGTGAAATTGAAGAAATTCCAGCATTTATTGAGTTCAAAGATATTGAGGTAGTGATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAg                <  1:210818/141‑1 (MQ=255)                                                                                                                                tgtAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGgtgt            <  1:424604/141‑1 (MQ=255)                                                                                                                                 ctAGATCGAAAATAC‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTa           <  1:1389/140‑1 (MQ=255)                                                                                                                                    gATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCATAATCCAAATCACTAAATGAAGCTTTAGGTGTaaaa        <  1:414151/141‑1 (MQ=255)                                                                                                                                      tCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAat      <  1:205618/141‑1 (MQ=255)                                                                                                                                          aaaTAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAattatt  <  2:280954/141‑1 (MQ=255)                                                                                                                                             |                                                                                                                                            TCTTAGTCGGTGTACATGCTCAAGATGATAAGCAATTTAATTTTGAGTCTACAATGGAGGAATTATCATCTTTATCAGAGACTTGCCAACTTGAAGTGTTGGGTCAAATTACTCAAAACAGAGATCGTGTAGATCGCAAATAT‑TATGT‑TGGTAAAGGTAAAATTGAAGAAATTCAAGCATTTATTGAGTTCAAAGATATTGATGTAGTCATCACAAATGATGAATTAACGACTGCACAATCCAAATCACTAAATGAAGCTTTAGGTGTAAAAATTATT  >  CP000730/1333649‑1333926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑