BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,864	A→T	17.3%	intergenic (‑947/‑221)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,864	0	A	T	17.3%	412.7 / 62.4	172	intergenic (‑947/‑221)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (79/62); new base T (16/14); total (96/76)
Fisher's exact test for biased strand distribution p-value = 8.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

Only 100 of 178 total aligned reads displayed.

AGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCA  >  CP000730/1929725‑1930004

aGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑aa                                                                                                                                                >  1:156165/1‑141 (MQ=255)

aGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGAAA‑ACAAGTTTGACGGCGAG‑AGGAGGTGTa‑a‑aa                                                                                                                                                >  1:292766/1‑141 (MQ=255)

 gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGCGTA‑T‑CA‑g                                                                                                                                              >  2:100176/1‑141 (MQ=255)

 gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACCCAGAG‑CGGCGGTGTA‑A‑AA‑g                                                                                                                                              >  2:205334/1‑141 (MQ=255)

  tgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑g‑g                                                                                                                                            <  1:601325/141‑1 (MQ=255)

   gtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGGGTA‑T‑AA‑g‑gg                                                                                                                                           >  1:246895/1‑140 (MQ=255)

        cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGc                                                                                                                                     >  1:386619/1‑141 (MQ=255)

                  tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGt                                                                                                                           >  1:32723/1‑141 (MQ=255)

                  tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGt                                                                                                                           >  2:624848/1‑141 (MQ=255)

                               cATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGCTGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGTAGGTGTA‑T‑AA‑G‑G‑TGCTGCTTATTTTCGTTCACATCATAGCa                                                                                                              >  1:251606/1‑141 (MQ=255)

                                             atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                               <  1:117662/127‑1 (MQ=255)

                                             atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                               >  2:561683/15‑141 (MQ=255)

                                              tcggaagtagatatgCTTGTCTATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                              >  1:471002/14‑141 (MQ=255)

                                              tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                              >  1:189291/14‑141 (MQ=255)

                                                ggaagtagatacgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                            <  2:505844/129‑1 (MQ=255)

                                                 gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                           >  2:606190/11‑141 (MQ=255)

                                                 gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGCCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                           <  2:134865/131‑1 (MQ=255)

                                                  aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                          >  1:29408/10‑141 (MQ=255)

                                                  aaGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCCCCAGCCATCAGTGGCtgtg                                                                                           >  2:61050/1‑141 (MQ=255)

                                                  aaGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                           <  2:446337/141‑1 (MQ=255)

                                                   agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                         >  1:613140/9‑140 (MQ=255)

                                                   actagatacgCTTGTATATTCACAGACGGTATAATAATAGAGTTGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCCCATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                         <  1:192797/132‑2 (MQ=255)

                                                   aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                          <  1:742496/141‑1 (MQ=255)

                                                    gTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGAGGCGGGTTTTCGTTCACATCATGGCACCAGTCATCAGTGGCTGTGcc                                                                                         >  1:524609/1‑141 (MQ=255)

                                                     tagatatgCTTGTATATTCACAGATGGTATAAGAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                       <  2:464364/135‑4 (MQ=255)

                                                      agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                                                      >  1:550046/6‑137 (MQ=255)

                                                       gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                     >  1:24742/5‑136 (MQ=255)

                                                       gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                     >  1:646252/5‑136 (MQ=255)

                                                       ccggtgCTTGGAGATTTGCCGATGATATAATAGTAGAGTAGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGCGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCCTCAGTGGCTGTGCCAtt                                                                                      <  2:209532/138‑1 (MQ=255)

                                                       aCTGTGCTTGTATATTTGCCTATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGGAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                      <  2:315977/141‑1 (MQ=255)

                                                        atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgg                                                                                    >  1:534432/4‑135 (MQ=255)

                                                        atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                                                    <  1:315760/138‑7 (MQ=255)

                                                        atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                                                    <  1:684803/138‑7 (MQ=255)

                                                         tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGAGCTGTTGCg                                                                                   <  1:567487/139‑1 (MQ=255)

                                                         tatgCTTGTATATGCACAGATGGTAAAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                                   <  1:698596/139‑1 (MQ=255)

                                                         tatgCTTGTATACTCACAGAAGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                                   <  1:567610/139‑1 (MQ=255)

                                                          gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg                                                                                   >  2:402339/1‑141 (MQ=255)

                                                          gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCT‑AAAGAAGCAAAAGTGAGCCG‑AGTGAACGTCGAGTA‑T‑AA‑G‑G‑TGATGCTTGTTTTCGTTCACATCAGAGCACCAGTCATCAGTGGCTGTACCATTGCg                                                                                   >  1:370522/1‑141 (MQ=255)

                                                          atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                  <  1:105831/140‑1 (MQ=255)

                                                            gCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtt                                                                                 >  1:691625/1‑141 (MQ=255)

                                                             cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                               >  2:642691/1‑141 (MQ=255)

                                                               gttattTTGGCCGATGATATAATAGTAGAGTCGCTTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑T‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                                                                              <  2:395127/139‑1 (MQ=255)

                                                                gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                            <  2:558452/141‑7 (MQ=255)

                                                                   tatTTACAGATGGTGTAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatactt                                                                         <  1:198709/141‑7 (MQ=255)

                                                                     tttGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCA‑TCAATTTAGACGCAGAG‑ACACGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCACCAGTGACTGTTCCCTTGCGtttttttactt                                                                        >  2:174548/1‑137 (MQ=255)

                                                                     ttcacagatgatATAAAAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                       <  2:23651/135‑1 (MQ=255)

                                                                      tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                      >  1:734401/6‑141 (MQ=255)

                                                                        acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTgg                                                                    <  2:585235/138‑1 (MQ=255)

                                                                        acagatgggataataataGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                    <  1:71719/132‑1 (MQ=255)

                                                                        acagaaggtataATAATAGAGTCGCCTAACTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                    >  1:390471/9‑141 (MQ=255)

                                                                             tggtataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCC‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                               >  1:297551/4‑141 (MQ=255)

                                                                             tggtataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                               >  1:430400/4‑141 (MQ=255)

                                                                             gggtataATAAGAGAGTCGCCTATCTCCCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCGAATCATTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                               <  1:261843/138‑4 (MQ=255)

                                                                              ggtataAAAATAGAGTCGCCAATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCGCATCATAGCACCAGTCATCAGTGGCTGTGCTGTTACGTATTATACTTATTGGCTTAGt                                                              >  1:488767/3‑141 (MQ=255)

                                                                                tataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                            <  1:538867/141‑2 (MQ=255)

                                                                                tataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                            >  2:668643/1‑140 (MQ=255)

                                                                                 ataataataGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTACTCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                           <  2:576585/141‑3 (MQ=255)

                                                                                    ataataGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTATACGc                                                        <  1:525159/141‑1 (MQ=255)

                                                                                    ataataGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                        <  2:158035/141‑1 (MQ=255)

                                                                                     taatagAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                       <  1:416749/138‑1 (MQ=255)

                                                                                      aatagATTCGCCTATCTCTCAGGCG‑TCAA‑‑TGGACTAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                      <  2:770001/139‑1 (MQ=255)

                                                                                      aatagAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                      <  2:749139/139‑1 (MQ=255)

                                                                                        tagAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaaa                                                    >  1:31180/1‑139 (MQ=255)

                                                                                        tagAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                                    >  2:758991/1‑139 (MQ=255)

                                                                                        tagAGTCGCCTATCCCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                                    <  2:264399/141‑3 (MQ=255)

                                                                                         agagTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATa                                                    <  2:478470/141‑1 (MQ=255)

                                                                                         agagTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTACACGCaacaa                                                   >  2:416835/1‑138 (MQ=255)

                                                                                         agagTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                   <  1:634147/141‑4 (MQ=255)

                                                                                           agTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                 >  2:72282/1‑136 (MQ=255)

                                                                                           agTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                 >  2:480994/1‑136 (MQ=255)

                                                                                            gTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                                                 <  2:32723/141‑1 (MQ=255)

                                                                                            gTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                                                 <  1:492273/141‑1 (MQ=255)

                                                                                            gTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                                                 <  2:698408/141‑1 (MQ=255)

                                                                                               gCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTACACGCaacaaatgatt                                             >  1:244536/1‑132 (MQ=255)

                                                                                               gCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                             <  2:231325/141‑10 (MQ=255)

                                                                                               gCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                             >  2:24855/1‑132 (MQ=255)

                                                                                               gCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                             <  2:155011/141‑10 (MQ=255)

                                                                                               gCCTATCTCTAAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                             <  2:284388/141‑10 (MQ=255)

                                                                                                ccTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATa                                             >  2:193899/1‑141 (MQ=255)

                                                                                                ccTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCC‑TCAC‑G‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCACCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataataa                                            >  1:730734/1‑131 (MQ=255)

                                                                                                ccTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                            >  1:667696/1‑131 (MQ=255)

                                                                                                ccTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                            >  1:457055/1‑131 (MQ=255)

                                                                                                 cTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                           >  2:101946/1‑130 (MQ=255)

                                                                                                 cTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                           >  1:320741/1‑130 (MQ=255)

                                                                                                 cTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                           >  2:85138/1‑130 (MQ=255)

                                                                                                  tATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                          <  2:613140/141‑13 (MQ=255)

                                                                                                   aTCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                         >  1:325699/1‑128 (MQ=255)

                                                                                                   aTCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataagtaata                                         >  1:171442/1‑128 (MQ=255)

                                                                                                   aTCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                         >  2:666719/1‑128 (MQ=255)

                                                                                                        tcAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACat                                     >  1:608235/1‑141 (MQ=255)

                                                                                                        ccAGGCG‑GCAATTTAGACGCAGAG‑AGGAGGGGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAGTGGCTAAGTAGACGCAATACAAAATAGGTGACat                                     <  1:342103/140‑1 (MQ=255)

                                                                                                                tCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATCGCCg                              >  2:644272/1‑141 (MQ=255)

                                                                                                                        gACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATa                      >  1:456067/1‑141 (MQ=255)

                                                                                                                           gctgag‑cgGAGGTGTA‑T‑AA‑G‑G‑GGATGCTTATTTTCGTTCCCATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaa                   <  2:379884/138‑1 (MQ=255)

                                                                                                                            cAGAG‑CGGAACTGTG‑T‑AA‑G‑C‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaaa                  <  1:756816/141‑1 (MQ=255)

                                                                                                                                g‑aggagGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATccc              <  1:624848/141‑1 (MQ=255)

                                                                                                                                  aggaggTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAGTAAACATcccc             >  1:588547/1‑141 (MQ=255)

                                                                                                                                    gaggTGTA‑T‑AA‑G‑G‑TGATGATAGGGTGCTGTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTc           >  2:748512/1‑141 (MQ=255)

                                                                                                                                      gtgtta‑t‑aA‑G‑G‑TGATGCTTATTTTCTGTCACATCATAGCACCATTCATCAGTGGCTGCGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGTACCAATAAAAATCCCCTAAc         <  2:299320/137‑1 (MQ=255)

                                                                                                                                            tt‑aA‑G‑G‑TCGTGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCg    <  2:539066/140‑1 (MQ=255)

AGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCA  >  CP000730/1929725‑1930004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`