BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,875	T→C	67.6%	intergenic (‑958/‑210)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,875	0	T	C	67.6%	235.8 / 121.9	172	intergenic (‑958/‑210)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (30/26); new base C (62/52); total (94/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

AATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGG  >  CP000730/1929739‑1930015

   cTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCg                                                                                                                                       <  1:534176/141‑1 (MQ=255)

    tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGt                                                                                                                                      >  2:624848/1‑141 (MQ=255)

                 cATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGCTGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGTAGGTG‑TA‑T‑AA‑G‑G‑TGCTGCTTATTTTCGTTCACATCATAGCa                                                                                                                         >  1:251606/1‑141 (MQ=255)

                    ataACTTATCAGAAGCAAGAGACTGTGCTTGTATATTTGCCGATGATATAATAGCAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCa                                                                                                                      >  1:503233/1‑141 (MQ=255)

                               atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                          >  2:561683/15‑141 (MQ=255)

                               atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                          <  1:117662/127‑1 (MQ=255)

                               atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                          >  1:307206/15‑141 (MQ=255)

                                tcggaagtagatatgCTTGTCTATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                                         >  1:471002/14‑141 (MQ=255)

                                tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                                         >  1:189291/14‑141 (MQ=255)

                                 cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCGATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                                        >  1:456350/13‑141 (MQ=255)

                                  ggaagtagatacgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                                       <  2:505844/129‑1 (MQ=255)

                                   gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                                      >  2:606465/11‑141 (MQ=255)

                                   gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                                      >  2:606190/11‑141 (MQ=255)

                                    aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                                     >  1:29408/10‑141 (MQ=255)

                                    aaGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                                      <  2:446337/141‑1 (MQ=255)

                                     agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                                    >  1:613140/9‑140 (MQ=255)

                                     aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                                     <  1:742496/141‑1 (MQ=255)

                                      gtagatatgCCTGTATACTCACAGATGGTACAATAATAGCGTCGCCCACCTCGCGTCCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                                   <  1:21780/134‑3 (MQ=255)

                                       tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                                  <  1:316784/135‑4 (MQ=255)

                                       tagatatgCTTGTATATTCACAGATGGTATAAGAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                                  <  2:464364/135‑4 (MQ=255)

                                       atgatatgattGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                                  <  2:156768/132‑4 (MQ=255)

                                         gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                                >  1:646252/5‑136 (MQ=255)

                                         ccggtgCTTGGAGATTTGCCGATGATATAATAGTAGAGTAGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGCG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCCTCAGTGGCTGTGCCAtt                                                                                                 <  2:209532/138‑1 (MQ=255)

                                         aCTGTGCTTGTATATTTGCCTATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGGAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                                                                                                 <  2:315977/141‑1 (MQ=255)

                                           tgtgCTTGTATATTCGCCGATGATATAATAGGAGAGTCGCCTATCTCTCCGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                               <  2:28513/141‑1 (MQ=255)

                                           tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGAGCTGTTGCg                                                                                              <  1:567487/139‑1 (MQ=255)

                                           tatgCTTGTATATGCACAGATGGTAAAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                                              <  1:698596/139‑1 (MQ=255)

                                            gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGGGGCG‑GA‑C‑AA‑G‑C‑AGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATGGCg                                                                                              >  1:177912/1‑141 (MQ=255)

                                            gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCT‑AAAGAAGCAAAAGTGAGCCG‑AGTGAACGTCGAG‑TA‑T‑AA‑G‑G‑TGATGCTTGTTTTCGTTCACATCAGAGCACCAGTCATCAGTGGCTGTACCATTGCg                                                                                              >  1:370522/1‑141 (MQ=255)

                                            atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                             <  1:105831/140‑1 (MQ=255)

                                               cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                                          >  2:642691/1‑141 (MQ=255)

                                                 gttattTTGGCCGATGATATAATAGTAGAGTCGCTTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑T‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                                                                                         <  2:395127/139‑1 (MQ=255)

                                                  gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                                       <  1:538346/141‑7 (MQ=255)

                                                  gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                                       <  2:558452/141‑7 (MQ=255)

                                                   tataTTCACAGATGGTATAATAATAGATTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACCAAGAG‑AGGAGGTG‑CA‑T‑TA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                      <  1:365937/141‑8 (MQ=255)

                                                      attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGCTGTTGCGTATTATACttt                                                                                   >  2:170795/8‑140 (MQ=255)

                                                      aattaccaatgatATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCtt                                                                                    <  1:333251/133‑1 (MQ=255)

                                                       tttGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCA‑TCAATTTAGACGCAGAG‑ACACGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCACCAGTGACTGTTCCCTTGCGtttttttactt                                                                                   >  2:174548/1‑137 (MQ=255)

                                                       ttcacagatgatATAAAAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                  <  2:23651/135‑1 (MQ=255)

                                                        tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                 >  1:736519/6‑141 (MQ=255)

                                                          acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                               >  1:687411/4‑141 (MQ=255)

                                                          acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTgg                                                                               <  2:585235/138‑1 (MQ=255)

                                                          acagatgggataataataGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                               <  1:71719/132‑1 (MQ=255)

                                                          acagaaggtataATAATAGAGTCGCCTAACTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                               >  1:390471/9‑141 (MQ=255)

                                                             gatggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                            <  1:165398/141‑2 (MQ=255)

                                                              atggtATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta                                                                           <  2:753092/141‑3 (MQ=255)

                                                               tggtataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                          <  2:425958/138‑4 (MQ=255)

                                                               tggtataATAATAGAGGCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGCAGAG‑AGGAGGAG‑CATT‑AC‑T‑T‑TGCTAATCATTTTAGTTCACATAATAGCACGAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                          >  1:646422/4‑141 (MQ=255)

                                                               gggtataATAAGAGAGTCGCCTATCTCCCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCGAATCATTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                          <  1:261843/138‑4 (MQ=255)

                                                                ggtataATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                         >  1:582952/3‑141 (MQ=255)

                                                                ggtataAAAATAGAGTCGCCAATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCGCATCATAGCACCAGTCATCAGTGGCTGTGCTGTTACGTATTATACTTATTGGCTTAGt                                                                         >  1:488767/3‑141 (MQ=255)

                                                                  tataATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTa                                                                        >  2:697637/1‑141 (MQ=255)

                                                                   ataataataGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTACTCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                      <  2:576585/141‑3 (MQ=255)

                                                                      ataataGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTATACGc                                                                   <  1:525159/141‑1 (MQ=255)

                                                                      ataataGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                   <  1:293933/141‑1 (MQ=255)

                                                                      ataGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACg                                                                    <  1:247138/141‑1 (MQ=255)

                                                                       taatagAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                                  <  1:416749/138‑1 (MQ=255)

                                                                        aatagATTCGCCTATCTCTCAGGCG‑TCAA‑‑TGGACTAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                                 <  2:770001/139‑1 (MQ=255)

                                                                          tagAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                                               >  2:758991/1‑139 (MQ=255)

                                                                          tagAGTCGCCTATCCCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                                               <  2:264399/141‑3 (MQ=255)

                                                                          atgagTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                                               <  1:544764/139‑3 (MQ=255)

                                                                           agagTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATa                                                               <  2:668581/141‑1 (MQ=255)

                                                                           agagTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATa                                                               <  2:478470/141‑1 (MQ=255)

                                                                           agagTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                              <  1:244552/141‑4 (MQ=255)

                                                                           agagTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                              <  1:634147/141‑4 (MQ=255)

                                                                            gagTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                             >  1:59597/1‑137 (MQ=255)

                                                                            gagTCGCCTATCCCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                              <  2:41598/141‑1 (MQ=255)

                                                                             agTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                            >  2:72282/1‑136 (MQ=255)

                                                                             agTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CA‑T‑TACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                            >  2:480994/1‑136 (MQ=255)

                                                                              gTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                                                            <  1:492273/141‑1 (MQ=255)

                                                                              gTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                                                            <  2:698408/141‑1 (MQ=255)

                                                                              gTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaa                                                            <  2:32723/141‑1 (MQ=255)

                                                                              gTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACCAAGCG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTCCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                           <  1:315589/141‑7 (MQ=255)

                                                                              gTCGCCGATCTCTCAGGCG‑TCAATT‑‑GAAGAAGAG‑AGGAGGTGCGT‑T‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                           >  2:196975/1‑135 (MQ=255)

                                                                               tCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataa                                                          >  2:113229/1‑134 (MQ=255)

                                                                                 gCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                        <  2:154858/141‑10 (MQ=255)

                                                                                 gCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                        <  2:231325/141‑10 (MQ=255)

                                                                                 gCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                        >  2:24855/1‑132 (MQ=255)

                                                                                 gCCTATCTCTAAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                        <  2:284388/141‑10 (MQ=255)

                                                                                  ccTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CC‑TCAC‑G‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCACCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataataa                                                       >  1:730734/1‑131 (MQ=255)

                                                                                  ccTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                       >  1:667696/1‑131 (MQ=255)

                                                                                   cTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                      >  1:320741/1‑130 (MQ=255)

                                                                                   cTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                      >  2:101946/1‑130 (MQ=255)

                                                                                   cTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                      >  2:85138/1‑130 (MQ=255)

                                                                                    tATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                     <  2:613140/141‑13 (MQ=255)

                                                                                    tATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaaa                                                     >  1:694320/1‑129 (MQ=255)

                                                                                     aTCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTG‑CATT‑AC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                    >  1:325699/1‑128 (MQ=255)

                                                                                          tcAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACat                                                >  1:608235/1‑141 (MQ=255)

                                                                                                  tCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATCGCCg                                         >  2:644272/1‑141 (MQ=255)

                                                                                                          gACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATa                                 >  1:456067/1‑141 (MQ=255)

                                                                                                             gctgag‑cgGAGGTG‑TA‑T‑AA‑G‑G‑GGATGCTTATTTTCGTTCCCATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaa                              <  2:379884/138‑1 (MQ=255)

                                                                                                              cAGAG‑CGGAACTG‑TG‑T‑AA‑G‑C‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaaa                             <  1:756816/141‑1 (MQ=255)

                                                                                                                  g‑aggagGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATccc                         <  1:624848/141‑1 (MQ=255)

                                                                                                                    aggaggTG‑TG‑G‑AA‑G‑G‑TGATGCTTAGTTTCGTTCACATCATAGCACCCGTCATCAGGGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATCCAAAATAGGTGACATATAGCCGCACCAATAAAAATcctc                        >  1:566864/1‑139 (MQ=255)

                                                                                                                      gaggTG‑TA‑T‑AA‑G‑G‑TGATGATAGGGTGCTGTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTc                      >  2:748512/1‑141 (MQ=255)

                                                                                                                               tt‑aA‑G‑G‑TCGTGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCg               <  2:539066/140‑1 (MQ=255)

                                                                                                                                  aa‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCa             >  1:193055/1‑141 (MQ=255)

                                                                                                                                   a‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCaa            >  1:489336/1‑141 (MQ=255)

                                                                                                                                        gttaTGCTTGTTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAAt          <  2:638656/138‑1 (MQ=255)

                                                                                                                                                tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAggg  <  2:370522/141‑1 (MQ=255)

AATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTG‑TA‑T‑AA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGG  >  CP000730/1929739‑1930015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`