BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,946	G→A	43.4%	intergenic (‑1029/‑139)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,946	0	G	A	43.4%	42.5 / 115.1	107	intergenic (‑1029/‑139)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (28/31); new base A (28/17); total (58/49)
Fisher's exact test for biased strand distribution p-value = 1.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.40e-01

Only 100 of 107 total aligned reads displayed.

ATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCTTTTTGTA  >  CP000730/1929809‑1930080

ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTATACGc                                                                                                                                              <  1:525159/141‑1 (MQ=255)

ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                                              <  2:158035/141‑1 (MQ=255)

ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                                              <  1:293933/141‑1 (MQ=255)

ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACg                                                                                                                                               <  1:247138/141‑1 (MQ=255)

agtatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                                              <  1:632896/137‑1 (MQ=255)

 taatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                                                                                                             <  1:416749/138‑1 (MQ=255)

  aatagATTCGCCTATCTCTCAGGCGTCAA‑‑TGGACTAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑a                                                                                                                                          <  2:770001/139‑1 (MQ=255)

  aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑a                                                                                                                                          <  2:749139/139‑1 (MQ=255)

   atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑ac                                                                                                                                         >  2:565761/2‑140 (MQ=255)

    tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑aaa                                                                                                                                        >  1:31180/1‑139 (MQ=255)

    tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑aca                                                                                                                                        >  2:758991/1‑139 (MQ=255)

    tagAGTCGCCTATCCCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑aca                                                                                                                                        <  2:264399/141‑3 (MQ=255)

    atgagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑aca                                                                                                                                        <  1:544764/139‑3 (MQ=255)

     agagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑a                                                                                                                                      <  2:668581/141‑1 (MQ=255)

     agagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑a                                                                                                                                      <  2:478470/141‑1 (MQ=255)

     agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTACACGCa‑‑acaa                                                                                                                                       >  2:416835/1‑138 (MQ=255)

     agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaa                                                                                                                                       <  1:634147/141‑4 (MQ=255)

     agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaa                                                                                                                                       <  1:244552/141‑4 (MQ=255)

      gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaa                                                                                                                                      >  1:59597/1‑137 (MQ=255)

      gagTCGCCTATCCCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑Ac                                                                                                                                     <  2:41598/141‑1 (MQ=255)

       agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaat                                                                                                                                     >  2:72282/1‑136 (MQ=255)

       agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaat                                                                                                                                     >  2:480994/1‑136 (MQ=255)

        gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACaa                                                                                                                                   <  2:698408/141‑1 (MQ=255)

        gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACaa                                                                                                                                   <  1:72687/141‑1 (MQ=255)

        gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACaa                                                                                                                                   <  1:492273/141‑1 (MQ=255)

        gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACaa                                                                                                                                   <  2:32723/141‑1 (MQ=255)

        gTCGCCTATCTCTCAGGCGTCAATT‑‑GACCAAGCGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTCCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaata                                                                                                                                    <  1:315589/141‑7 (MQ=255)

        gTCGCCGATCTCTCAGGCGTCAATT‑‑GAAGAAGAGAGGAGGTGCGT‑TAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaata                                                                                                                                    >  2:196975/1‑135 (MQ=255)

         tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataa                                                                                                                                   >  2:113229/1‑134 (MQ=255)

           gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTACACGCa‑‑acaaatgatt                                                                                                                                 >  1:244536/1‑132 (MQ=255)

           gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                                                 >  2:730510/1‑132 (MQ=255)

           gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                                                 <  2:231325/141‑10 (MQ=255)

           gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                                                 >  1:287339/1‑132 (MQ=255)

           gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                                                 >  2:24855/1‑132 (MQ=255)

           gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                                                 <  2:154858/141‑10 (MQ=255)

           gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                                                 <  2:155011/141‑10 (MQ=255)

           gCCTATCTCTAAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                                                 <  2:284388/141‑10 (MQ=255)

            ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATa                                                                                                                             >  2:193899/1‑141 (MQ=255)

            ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatta                                                                                                                                >  1:491941/1‑131 (MQ=255)

            ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatta                                                                                                                                >  1:667696/1‑131 (MQ=255)

            ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatta                                                                                                                                >  2:514869/1‑131 (MQ=255)

            ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatta                                                                                                                                >  1:457055/1‑131 (MQ=255)

             cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                                               >  1:744668/1‑130 (MQ=255)

             cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                                               >  1:320741/1‑130 (MQ=255)

             cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                                               >  2:101946/1‑130 (MQ=255)

              tATCTCTCAGGCGTCAATT‑‑GTCGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaat                                                                                                                              <  2:71437/141‑13 (MQ=255)

              tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaat                                                                                                                              <  2:613140/141‑13 (MQ=255)

              tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaaa                                                                                                                              >  1:694320/1‑129 (MQ=255)

               aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                                             >  2:277348/1‑128 (MQ=255)

               aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                                             >  1:325699/1‑128 (MQ=255)

               aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataagtaata                                                                                                                             >  1:171442/1‑128 (MQ=255)

               aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                                             >  2:666719/1‑128 (MQ=255)

                tctctcAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTTTTTTTTTTTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTg                                                                                                                         >  2:205758/1‑141 (MQ=255)

                    tcAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑a‑t                                                                                                                   >  1:608235/1‑141 (MQ=255)

                    tcAGGCGTCAATTTAGACGCAGAGAGGAGGAG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTAGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑CATGGGTGGC‑a‑t                                                                                                                   >  2:256390/1‑141 (MQ=255)

                    ccAGGCGGCAATTTAGACGCAGAGAGGAGGGG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAGTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑a‑t                                                                                                                   <  1:342103/140‑1 (MQ=255)

                      aGGCGTCAATTTAGACGCAGAGAGGAGGGG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGGGGC‑a‑tat                                                                                                                 >  2:587540/1‑141 (MQ=255)

                           tCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑CGCC‑g                                                                                                          >  2:644272/1‑141 (MQ=255)

                             aaTTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑TCAA‑‑AATAGGTGAG‑A‑TAT‑AGCC‑GCa                                                                                                        >  2:341349/1‑141 (MQ=255)

                                   gACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATa                                                                                                  >  1:456067/1‑141 (MQ=255)

                                    aCGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTGTTATTCGGCCTGCCTAAATCGGCGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCCCCAAGaa                                                                                                 >  2:316106/1‑141 (MQ=255)

                                       cAGAGCGGAACTG‑TG‑TAA‑G‑C‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATaaaaa                                                                                              <  1:756816/141‑1 (MQ=255)

                                           gaggagGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATccc                                                                                          <  1:624848/141‑1 (MQ=255)

                                            aggaggTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAGTAAACATcccc                                                                                         >  1:588547/1‑141 (MQ=255)

                                              gaggTG‑TA‑TAA‑G‑G‑TGATGATAGGGTGCTGTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTc                                                                                       >  2:748512/1‑141 (MQ=255)

                                                 gtgtta‑taA‑G‑G‑TGATGCTTATTTTCTGTCACATCATAGCACCATTCATCAGTGGCTGCGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GTACCAATAAAAATCCCCTAAc                                                                                     <  2:299320/137‑1 (MQ=255)

                                                       ttaA‑G‑G‑TCGTGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCg                                                                                <  2:539066/140‑1 (MQ=255)

                                                         aa‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCa                                                                              >  1:193055/1‑141 (MQ=255)

                                                          a‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCaa                                                                             >  1:489336/1‑141 (MQ=255)

                                                               gttaTGCTTGTTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAAt                                                                           <  2:638656/138‑1 (MQ=255)

                                                                   tGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTATTTCTAATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAgag                                                                       >  1:658523/1‑139 (MQ=255)

                                                                   tGCTTATATTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AG‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAGTCCCCTCACTACCGCAAATAGTg                                                                       >  1:487649/1‑141 (MQ=255)

                                                                      ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAACAGTGAgg                                                                    >  1:49664/1‑141 (MQ=255)

                                                                       tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAggg                                                                   <  2:370522/141‑1 (MQ=255)

                                                                           ttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGAtt                                                               <  1:276995/141‑1 (MQ=255)

                                                                            ccgctCACATCATCGCTCTTGTCATGAGTGGCTGTGCCATTGCGTTTTTTTCGTCTGTGCTAAGTAGACGCA‑‑AT‑‑ATAA‑‑AACAGGTGGC‑A‑TAT‑AGCC‑TCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTg                                                              <  2:213888/137‑1 (MQ=255)

                                                                             cGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTgg                                                             >  1:237177/1‑141 (MQ=255)

                                                                             cGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACCCA‑‑AA‑‑ACAA‑‑AATCGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAAGGGATTgg                                                             >  1:626273/1‑141 (MQ=255)

                                                                               ttCACATCATAGCACCAGTCATCAGTGGCGGTGCGATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGGGAC‑A‑TAC‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGCTTGgtg                                                           <  2:268565/141‑1 (MQ=255)

                                                                                   catcatAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGtata                                                       >  2:291179/1‑141 (MQ=255)

                                                                                       atAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAACAA‑‑ATAATTAATAGGCGACTT‑TATAAGCCAGCATCGA‑AAAAATCCCCACACTACTGC‑AATAGTGAGGGGATTGGTGcat                                                        >  1:484282/1‑141 (MQ=255)

                                                                                        tAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCA‑‑ACAAATAATTAATAGGCGAC‑TTTATAAGCCAGCATCGA‑AAAAATCCCCACACTACTGC‑AATAGTGTGGGGATTGGTGcatt                                                       >  2:677055/1‑140 (MQ=255)

                                                                                          gCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑GATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAAt                                                >  1:423073/1‑141 (MQ=255)

                                                                                           cACCGGTCATCAGTGGCTGTGCCATGGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAACTTCCCCTCACTTCCGCCAATAGTGAGGGGATTGGTGTATCAGTACATa                                               <  2:377276/141‑1 (MQ=255)

                                                                                               aGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑ACTAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGCTTGGTGTCTAAGTAAATACTTa                                           >  2:118788/1‑141 (MQ=255)

                                                                                               aGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTCTAAGTAAATACTTa                                           >  2:118702/1‑141 (MQ=255)

                                                                                                 tcatcaGTGGCTGTGCCATTGCGTTTTTTTCTTCTTGGCTAAGTAGACGCA‑‑AT‑‑GCCA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCATTACCGCAAATAGTGAGGGGATTGTTGTATAAGTAAATACTTAtt                                         <  2:423686/141‑1 (MQ=255)

                                                                                                 tcatcaGTGGCTGGGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAtt                                         <  2:386619/141‑1 (MQ=255)

                                                                                                 gcatcaGTGGCTGTGCCATTGCGTTTTTTGCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAtt                                         <  1:61050/140‑1 (MQ=255)

                                                                                                  catcaGTGGCTGTGCCATTGCGTTTTTTTTTTTTTTTCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                                        >  2:302004/1‑141 (MQ=255)

                                                                                                       gTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGtt                                   <  1:724184/141‑1 (MQ=255)

                                                                                                       gTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGtt                                   <  1:452381/141‑1 (MQ=255)

                                                                                                       gTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AAAAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAACTACTTATTTTCGtt                                   >  2:627244/1‑141 (MQ=255)

                                                                                                                      cGTTGTTTTCTTATTGGATAATTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCATAAATAAACATCCCCTCACTACCGGAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTg                    <  2:392736/141‑1 (MQ=255)

                                                                                                                          tttttCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTaa                <  1:455407/141‑1 (MQ=255)

                                                                                                                            tttCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAAtt              <  1:26577/141‑1 (MQ=255)

                                                                                                                              tCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAAtttt            <  2:608235/141‑1 (MQ=255)

                                                                                                                                 tATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCt         <  2:437386/141‑1 (MQ=255)

                                                                                                                                     ggCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCttttt     >  1:72177/1‑141 (MQ=255)

                                                                                                                                        tAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCTTTTTGTa  >  2:210345/1‑141 (MQ=255)

ATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑AT‑‑ACAA‑‑AATAGGTGAC‑A‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATTTTTCTTTTTGTA  >  CP000730/1929809‑1930080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`