Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,024,507 C→A 10.1% A37E (GCA→GAA)  USA300HOU_1878 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,024,5070CA10.1% 184.9 / 3.4 68A37E (GCA→GAA) USA300HOU_1878hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (26/33);  new base A (2/6);  total (28/40)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATGCAAATTTGAAAGAGGGGTAGTTTTTTATGGAAAATAAATTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGAACGTTCATTAAAGGATGCGAAAGAAACA  >  CP000730/2024367‑2024646
                                                                                                                                            |                                                                                                                                           
aGATGCAAATTTGAAAGAGGGGTAGTTTTTTATGGAAAATAAATTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGc                                                                                                                                             >  1:610196/1‑141 (MQ=255)
    gCAAATTTGAAAGAGGGGTAGTTTTTTATGGAAAATAAATTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGAaaaa                                                                                                                                         >  2:57074/1‑141 (MQ=255)
      aaaTTTGAAAGAGGGGTAGTTTTTTATGGAAAATAAATTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCaaaaaa                                                                                                                                       >  1:539052/1‑141 (MQ=255)
      aaaTTTGAAAGAGGGGTAGTTTTTTATGGAAAATAAATTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTGAGCTGATAGATCTAAACGTCAAGCTATAGATCAATCAGTTATAGATaaaaaaaa                                                                                                                                       >  2:445804/1‑141 (MQ=255)
       aaTTTGAAAGAGGGGTAGTTTTTTATGGAAAATAAATTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAAt                                                                                                                                      <  2:232375/141‑1 (MQ=255)
                     gTAGTTTTTTATGGAAAATAAATTTGTGCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCACTCCGTTCAAGATGCAAAAAATGGTAACCGCACTCg                                                                                                                        <  1:404875/141‑1 (MQ=255)
                      tAGTTTTTTATGGAAAATAAATTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTAAATCAGTTAAAGATGAAAAAAATGGTAACCGCACTAGt                                                                                                                       >  2:376844/1‑141 (MQ=255)
                       tgTTTTTTATGGATAATAAAGTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTa                                                                                                                      <  2:222817/140‑1 (MQ=255)
                       aGTTTTTTATGGAAAATAAATTTGTTCCTGGTATTTTAATTGGTGCCGTAATCGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGAAAAAAAAAAAAAAAATGGTAACCGCACTCGTa                                                                                                                      <  2:522074/141‑1 (MQ=255)
                        gTTTTTTATGGAAAATAAATTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGGTAAAGATGCAAAAAATGGTCACCGCACTCGTaa                                                                                                                     >  1:430991/1‑141 (MQ=255)
                         ttttttATGGAAAATAAATTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAg                                                                                                                    <  1:163068/141‑1 (MQ=255)
                         ttttttATGGAAAATAAATTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAg                                                                                                                    <  2:667263/141‑1 (MQ=255)
                         ttttttATGGAAAATAAATTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAg                                                                                                                    <  2:187485/141‑1 (MQ=255)
                         ttatttATGGAAAACAAATTTGTTTCTGGTATTCTAATGGGTGCAGTAATGGGTGGTGCAATAAGTTTATCTGATAAATCCACACGTCAATCTTTAGTACACTCAGTCAAAGATGCAAAAAATGGTAACCGCACTCGTAAg                                                                                                                    <  2:336575/138‑1 (MQ=255)
                          tttttATGGAAAATAAATTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGc                                                                                                                   >  1:340745/1‑141 (MQ=255)
                           ttttATGGAAAAAAAATTTGTTCCGGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGcc                                                                                                                  <  2:99195/141‑1 (MQ=255)
                              tATGGAAAATAAAGTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTc                                                                                                               <  2:168079/141‑1 (MQ=255)
                                   aaaaTATATTTGTTCCTGGTATTTTACTTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTCTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAg                                                                                                          <  2:447421/141‑1 (MQ=255)
                                    aaataaatTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGt                                                                                                         <  2:89578/141‑1 (MQ=255)
                                         aatTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGGAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAgta                                                                                                    >  1:149935/1‑139 (MQ=255)
                                         aatTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGgcagca                                                                                                    >  1:150406/1‑141 (MQ=255)
                                         aatTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCa                                                                                                    >  1:680060/1‑141 (MQ=255)
                                          atTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGAGGCAAAAAATGGTAACCGCACTCGTTAGCCTTCTAAAGTCAGCaa                                                                                                   <  1:423245/141‑1 (MQ=255)
                                          atTGGTTCCTGCAATTTTAATCGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCaa                                                                                                   <  1:204277/141‑1 (MQ=255)
                                             tGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGAt                                                                                                <  1:278228/141‑1 (MQ=255)
                                                  cTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAg                                                                                           <  2:495751/141‑1 (MQ=255)
                                                   aggTATTTTATTTGGGGCCGGAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCCTTAGTTCAGTAAGAAAAAAAAAAAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGa                                                                                          <  2:519187/140‑1 (MQ=255)
                                                    ggTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTACGCCTTCTAAAGTCCGCAAGATTAAAGAc                                                                                         <  1:505602/141‑1 (MQ=255)
                                                     gTATTTTAATTGGCGGCGTAATTGGCGGGGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAAAAAAAAAAAAAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACg                                                                                        <  2:693833/141‑1 (MQ=255)
                                                            aaTTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGtttt                                                                                 <  2:750509/141‑1 (MQ=255)
                                                                    ccGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATTAGTAAAAAAAAAAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGa                                                                         <  2:564958/141‑1 (MQ=255)
                                                                     cGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGaa                                                                        <  1:57074/141‑1 (MQ=255)
                                                                       tAATTGGGGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAg                                                                      <  2:208840/141‑1 (MQ=255)
                                                                        aagtggtggTGCCATTAGGTCAGCTGATAAATCTACACGTTAAGCTTTCGTTCAATCAGGTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTTTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGa                                                                     <  2:53456/138‑1 (MQ=255)
                                                                                gtgCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGttgttg                                                             >  2:188980/1‑141 (MQ=255)
                                                                                  gCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTgaa                                                           <  1:692955/141‑1 (MQ=255)
                                                                                  gCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTGGTTgaa                                                           >  1:588999/1‑141 (MQ=255)
                                                                                    aaTTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTgaaga                                                         <  2:241339/141‑1 (MQ=255)
                                                                                       tAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAAt                                                      <  2:595026/141‑1 (MQ=255)
                                                                                       tAGTTGACCTGATAAATCTAGACGTGGAGCTTCTGTTCAAAAAGTAAAAGATGAAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAAt                                                      <  2:740572/141‑1 (MQ=255)
                                                                                           ttAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATtcgt                                                  >  1:32132/1‑141 (MQ=255)
                                                                                              gCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTcgtcgt                                               <  2:233978/141‑1 (MQ=255)
                                                                                                 gATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAGTGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGtaat                                            <  1:559728/141‑1 (MQ=255)
                                                                                                 gATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGtaat                                            >  1:39047/1‑141 (MQ=255)
                                                                                                   tAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTCACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGCCGAAGTTTTATACTGGACAGCTGTTGTTCAAGCAACTCCTCGtcatca                                          >  2:772953/1‑139 (MQ=255)
                                                                                                   tAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGtaataa                                          >  2:772956/1‑141 (MQ=255)
                                                                                                       tCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTACCCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACAAAGTTTTATACTGGAAAGATGTTGTTAAAGAATTTCGTAGTAATAATCCt                                      >  2:310167/1‑141 (MQ=255)
                                                                                                       tCTACACGTCAAGCTTTAGTACAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAACCTTCTAAAGTCAGCAAGATCAAAAACAAACATTCATACTGGAAACCTATTGATAAAGAAATTAGACGCAATAATcat                                      >  2:213701/1‑139 (MQ=255)
                                                                                                       tCTACACGCCAAGCTTTAGTTCGATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCt                                      >  2:564775/1‑141 (MQ=255)
                                                                                                           cacCTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACCCCTAACCCTCCTGTTGGTAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAAt                                  <  1:367751/141‑1 (MQ=255)
                                                                                                           caagTCAACCGTTAGTTCAATGAGTGAAACAGGGAAAATATGGGAACCGCCCGCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGGTGTTGAAGAAATTCGTCTTAATACTCCTGAAt                                  <  2:367550/138‑1 (MQ=255)
                                                                                                               tCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAATTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGa                              <  1:23029/141‑1 (MQ=255)
                                                                                                               tCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGa                              <  2:340745/141‑1 (MQ=255)
                                                                                                               tCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGa                              <  1:667399/141‑1 (MQ=255)
                                                                                                                 aaGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGAAc                            >  1:93244/1‑141 (MQ=255)
                                                                                                                 aaGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGACGTAATAATCCTGAATTAGAAc                            >  2:106269/1‑141 (MQ=255)
                                                                                                                       tAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGAACGTTCAt                      >  2:293787/1‑141 (MQ=255)
                                                                                                                       tAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGAACGTTCAt                      >  1:673655/1‑141 (MQ=255)
                                                                                                                       tAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGAACGTTCAt                      >  1:206199/1‑141 (MQ=255)
                                                                                                                            gaaTCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGAACGTTCATTAAAg                 <  2:215716/140‑1 (MQ=255)
                                                                                                                            cAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGAACGTTCATTAAAg                 >  1:625149/1‑141 (MQ=255)
                                                                                                                             aaacAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAAGTCGGCGTAATAATCCTTAATTAGAACGTTCATTAAAgg                <  2:221587/138‑1 (MQ=255)
                                                                                                                             aaTCAGTAAAAAAAGAAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGAACGTTCATTAAAgg                <  2:599154/141‑1 (MQ=255)
                                                                                                                               tCAGTAAAAGATGCAAAAAATGGTAACCGCACGCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGAACGTTCATTAAAGGAt              <  1:711679/141‑1 (MQ=255)
                                                                                                                                cAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGAACGTTCATTAAAGGATg             >  2:602202/1‑141 (MQ=255)
                                                                                                                                     aaaGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATCATCCTGAATTAGAACGTTCATTAAAGGATGCgaaa        <  1:600302/141‑1 (MQ=255)
                                                                                                                                     aaaGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGAACGTTCATTAAAGGATGCgaaa        >  1:610571/1‑141 (MQ=255)
                                                                                                                                      aaGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGAACGTTCATTAAAGGATGCgaaag       >  1:200078/1‑141 (MQ=255)
                                                                                                                                        gATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGAACGTTCATTAAAGGATGGgaaagaa     >  2:496909/1‑141 (MQ=255)
                                                                                                                                           gCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGAACGTTCATTAAAGGATGCGAAAGAAACa  >  1:376229/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
AGATGCAAATTTGAAAGAGGGGTAGTTTTTTATGGAAAATAAATTTGTTCCTGGTATTTTAATTGGTGCCGTAATTGGTGGTGCAATTAGTTTAGCTGATAAATCTACACGTCAAGCTTTAGTTCAATCAGTTAAAGATGCAAAAAATGGTAACCGCACTCGTAAGCCTTCTAAAGTCAGCAAGATTAAAGACGAAGTTTTATACTGGAAAGATGTTGTTGAAGAAATTCGTCGTAATAATCCTGAATTAGAACGTTCATTAAAGGATGCGAAAGAAACA  >  CP000730/2024367‑2024646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: