BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	36,378	C→T	L58L (TTG→TTA)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,378	0	C	T	90.6%	3013.4 / 287.0	1564	L58L (TTG→TTA)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (77/69); new base T (707/695); total (794/770)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.97e-01

Only 100 of 1567 total aligned reads displayed.

GCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTA  >  CP000730/36233‑36525

      gctaCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                             <  2:868555/145‑1 (MQ=255)

      gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAACATTga                                                                                                                                             >  2:892480/1‑147 (MQ=255)

      gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                             <  1:45592/148‑1 (MQ=255)

      gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAAGCCATTTGTAATAAGCTTTTTTATGCTTTTTATTCCCAATTTTATATAAAATTgg                                                                                                                                             >  1:981665/1‑148 (MQ=11)

       cAACCAAATATCTACTGTATATCCATCTGCATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATCCTTTTTCTTCCAAATTTAATGTAAAATTtgg                                                                                                                                            >  2:398719/1‑148 (MQ=11)

         aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTCTTTTGTTTTACTTCTCATCAATACGCAAATTGTAATAATCTTTTTTATGCTTTTTCTTGCAAATTGGATATAAAATTgtggg                                                                                                                                          >  1:219519/1‑147 (MQ=11)

         aCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATGTACGTCGCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGc                                                                                                                                          <  2:942683/148‑1 (MQ=255)

          ccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCa                                                                                                                                         <  1:544181/148‑1 (MQ=37)

              atatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACACCATTTGTAATAAGCTTTCTTCGGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                                                                                                                                     >  1:953872/1‑148 (MQ=11)

                 tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCtt                                                                                                                                  <  2:975899/148‑1 (MQ=255)

                  cTAATGTATGTCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTCCAATTTTGATATAAAATTGGGGCATATTCTTg                                                                                                                                 >  2:776022/1‑148 (MQ=11)

                  cTAATGTATGTCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                                                                                                 >  2:1399524/1‑148 (MQ=255)

                   tAATGTATGTCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa                                                                                                                                <  2:29515/148‑1 (MQ=255)

                    aaTGTATGTCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                                                                                               <  1:1001937/148‑1 (MQ=255)

                    aaTGTATGTCCCTCTGCATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                                                                                               <  1:814437/148‑1 (MQ=37)

                    aaTGTATGTCCCTCTGCATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                                                                                               <  2:443271/148‑1 (MQ=37)

                     atgtatgtCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAATCtcacac                                                                                                                              >  1:251263/1‑143 (MQ=11)

                     atgtatgtCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAACTTGATATAAAATTGGGGCATATTCTTGaaa                                                                                                                              >  1:848599/1‑147 (MQ=255)

                     atgtatgtCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTCGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                                                                                              >  2:720336/1‑148 (MQ=255)

                      tgtatgtCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCTTATTCTTGAAcc                                                                                                                             >  2:811614/1‑148 (MQ=255)

                      tgtatgtCCCTCTGCATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATGGGGGCATATTCTTGAAcc                                                                                                                             >  1:1286093/1‑148 (MQ=255)

                       gtatgtCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATGGACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATTGGGGCGTATCCTCGAAccc                                                                                                                            >  2:1285761/1‑148 (MQ=11)

                        tatgtCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCa                                                                                                                           >  2:323719/1‑148 (MQ=255)

                        tatgtCCCTCTGCATCAATGGCACGATATAACATGGAACGA‑TTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAcaca                                                                                                                           >  1:1397681/1‑148 (MQ=11)

                        tatgtCCCTCTGCATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCa                                                                                                                           >  2:1057557/1‑148 (MQ=38)

                         atgtCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCaa                                                                                                                          <  2:1080046/148‑1 (MQ=255)

                           gtCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACg                                                                                                                        <  1:368145/148‑1 (MQ=255)

                           gtCCCTCTGTATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACg                                                                                                                        >  2:1201731/1‑148 (MQ=255)

                           gtCCCTCTGCATCAATGGCACGATATAA‑ATAGCTCCCTTTTCCTTTTATGGTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGTTACAAAATTGGGGCATATTCTTGAACCCAACg                                                                                                                        <  2:696860/148‑1 (MQ=255)

                            tCCGTCTGTATCAATGGCACGATATAA‑ATGGCACCATTTTCCTTTTATTTTGATATGCGTCTCGTCAATACGCCATTTGTAATAAACTCTTATAAGCTTTTTCTTCCAATTTTCATATAAAATTGCGGCATGTTGTGGAACTCAAccg                                                                                                                       >  2:596120/1‑146 (MQ=2)

                            tCCCTCTGCATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCATATTTGATATCATATTGGGGcttcttcttttactcacagg                                                                                                                       >  1:474990/1‑137 (MQ=11)

                                 cggtaTCAATGGCACGATATAA‑AGAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGat                                                                                                                  <  1:498488/144‑2 (MQ=11)

                                  tgtaTCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCCTATTCTTGAACCCAACGGCAGatc                                                                                                                 >  2:102570/4‑146 (MQ=11)

                                    taTCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGt                                                                                                               >  2:243172/2‑148 (MQ=255)

                                       tattGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                            <  1:597718/145‑1 (MQ=18)

                                       cAATGTCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                            <  2:1220475/148‑1 (MQ=255)

                                       cAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                            >  2:1368752/1‑148 (MQ=255)

                                       cAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                            >  1:172276/1‑148 (MQ=255)

                                        aaTGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                           >  1:520833/1‑148 (MQ=255)

                                        aaTGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                           >  2:227777/1‑148 (MQ=255)

                                          tGGCACGATATAA‑ATAGCTCCATTTTCCTGTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                         <  1:1128533/148‑1 (MQ=255)

                                              aCGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAAtgatga                                                                                                     >  1:1183532/1‑148 (MQ=21)

                                              aCGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                                     >  1:1312715/1‑148 (MQ=255)

                                              aCGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                                     >  1:652377/1‑148 (MQ=255)

                                               cGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGaa                                                                                                    <  1:31521/148‑1 (MQ=21)

                                                     aa‑aTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAACATTGGGGCATATTCTTGAACCCAATTGTTGATCGTTGAATGATGAACGTTTa                                                                                              <  1:1339098/148‑1 (MQ=11)

                                                          tgCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                          <  2:91337/147‑1 (MQ=21)

                                                          aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                          >  2:214273/1‑148 (MQ=255)

                                                          aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                          <  2:56906/148‑1 (MQ=255)

                                                           gCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                                         >  2:117161/1‑148 (MQ=21)

                                                           ccTCCATTTTCCTTTTATTTTGATTTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacca                                                                                         <  2:51359/147‑1 (MQ=21)

                                                            cgccATCTTCCTTTTCTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTCTGCTTTTTCTTCCAAATTTGATATAAAATTGGGACATCTTCTTGAACCCAACGGTACATCGTTGAATCATGAACGGTTAcaccag                                                                                        >  2:480872/3‑147 (MQ=11)

                                                            cTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                                                                                        >  1:627146/1‑148 (MQ=21)

                                                             tCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                                       >  1:999363/1‑148 (MQ=255)

                                                               cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt                                                                                     <  2:1258875/148‑1 (MQ=21)

                                                                atgttCCTTTTATTTTGATGTACGTCTTATCAATACGTCATTTGTAATGAGCTTTTTTATGCTTTTTCTTCTAAAGTGGATATGAAATTGGAGCATATTCTTGAACCGAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTc                                                                                    <  2:185188/145‑1 (MQ=11)

                                                                 ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTct                                                                                   <  2:112419/148‑2 (MQ=255)

                                                                  tttCCTTTTATTTTGATGTACGTCTCATCTATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                                  <  1:557543/148‑1 (MQ=18)

                                                                  tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAGCGTTTACACCACGTTctc                                                                                  <  1:1020983/148‑3 (MQ=11)

                                                                  tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctc                                                                                  <  2:666800/148‑3 (MQ=255)

                                                                  tttCCTTTTATTTGGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                                  <  1:151441/148‑1 (MQ=18)

                                                                  tttACTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctc                                                                                  <  1:739352/148‑3 (MQ=11)

                                                                   ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt                                                                                 <  1:1256887/148‑1 (MQ=21)

                                                                   ttCCCTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctct                                                                                 <  1:427179/148‑1 (MQ=11)

                                                                    tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                <  2:371408/148‑1 (MQ=11)

                                                                    tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                                <  1:1223278/148‑1 (MQ=255)

                                                                     ccTTTTACTTTGATATTTGTATTATTAATATGACATTTGTATTAAGATTTTTCATGCCTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTa                                                                               <  1:291154/148‑1 (MQ=11)

                                                                      cTTTTATTCTGAGGTACGTCTCATCAATACGCCAGCTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAAATGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                              <  1:20665/148‑1 (MQ=14)

                                                                       ttttATTTTGATATACGTCTCATCAATACGCCATATGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAATGGTAGATCGTTGAATGATGAACGTTTACCCTACGTTCTCTTAat                                                                             >  2:844145/1‑148 (MQ=11)

                                                                        tttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAata                                                                            >  1:793767/1‑148 (MQ=11)

                                                                          tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATAtt                                                                          <  1:1094725/148‑1 (MQ=21)

                                                                          tATTTTGATATACGTCTCACCAATCCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAtt                                                                          <  1:922564/148‑1 (MQ=11)

                                                                          tATTTTGAGATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTGTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATAtt                                                                          <  1:392056/148‑1 (MQ=11)

                                                                            ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTc                                                                        <  2:1347582/148‑1 (MQ=21)

                                                                            ttttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTc                                                                        <  1:1073986/148‑1 (MQ=21)

                                                                              ttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAg                                                                      >  2:530216/1‑148 (MQ=25)

                                                                               tGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGTACGTTTACACCACGTTCCCTTAATATTTCAGa                                                                     <  1:898403/148‑1 (MQ=21)

                                                                               tGATGTACGTCTCATCAATACGCCATTTCAACAGAGTGTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCCGa                                                                     >  2:647333/1‑148 (MQ=11)

                                                                                gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGCACGTTTACACCACGTTACCTTAATATTTCAGat                                                                    >  2:755313/1‑148 (MQ=255)

                                                                                  tatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCGGatat                                                                  >  2:274477/3‑148 (MQ=11)

                                                                                   atACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatata                                                                 >  1:577605/2‑148 (MQ=255)

                                                                                    tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatatat                                                                >  2:1142819/1‑148 (MQ=25)

                                                                                     tcGTCTCATCAATACGTCATTTGTAATAAGCTTTTTTATGCTCTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTGCCCTTAATATTTCAGATATATc                                                               <  2:422647/147‑1 (MQ=255)

                                                                                      cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                              >  1:135351/1‑148 (MQ=255)

                                                                                      cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                              >  1:1162997/1‑148 (MQ=40)

                                                                                       gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                             >  1:407874/1‑148 (MQ=11)

                                                                                       gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                             >  1:1370851/1‑148 (MQ=255)

                                                                                       gTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAc                                                             >  1:880461/1‑148 (MQ=21)

                                                                                              caATACGCCATTTGTAATAAGCTTTTTTATGCTTGTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGTCCGTTGAATGATGAACGTTTACATCACGTTCCCTTAATATTTCAGTTATATCACGATAACt                                                      <  2:621125/148‑1 (MQ=11)

                                                                                              caATACGACATTTGTAATAAGCTTTGTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                                      <  2:1103088/148‑1 (MQ=11)

                                                                                               aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTGCAGCACGTGCTCTTAATATTTCAGATATATCCCGATAGCTc                                                     >  1:33817/1‑148 (MQ=11)

                                                                                                  aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATCTAAAATTAGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                  <  1:1041115/148‑1 (MQ=11)

                                                                                                  aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAAt                                                  <  1:760310/148‑1 (MQ=11)

                                                                                                    gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgc                                                <  1:1205957/148‑2 (MQ=11)

                                                                                                     ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAATGGTAGACCGTTCAATGATGAACGTTTACACCACGTTCCCTTACTATTTCAGATATATCACGATAATTCACTgca                                               >  1:678096/1‑146 (MQ=11)

                                                                                                      cATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGcat                                              >  1:712894/1‑148 (MQ=17)

                                                                                                         ttttaataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATGcatacc                                           <  2:167525/145‑3 (MQ=11)

                                                                                                         ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATGCATATc                                           <  2:1312715/148‑1 (MQ=11)

                                                                                                                 aGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtag                                   >  2:1235525/1‑148 (MQ=38)

                                                                                                                   cTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagta                                 >  1:262099/1‑148 (MQ=37)

GCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAA‑ATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTA  >  CP000730/36233‑36525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`