Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,002,200 T→G M173R (ATG→AGG)  mecA2 → competence negative regulator MecA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,002,2000TG100.0% 304.6 / NA 87M173R (ATG→AGG) mecA2competence negative regulator MecA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (39/48);  total (39/48)

AGCTCAAGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGCTAAAA  >  CP000730/1002057‑1002340
                                                                                                                                                 |                                                                                                                                            
agCTCAAGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGtt                                                                                                                                          <  1:238349/148‑1 (MQ=255)
   tCAAGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTAAAGAGTTTGAAGATTTGTTATATAGGGTTGAt                                                                                                                                       >  2:198339/1‑148 (MQ=255)
     aaGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATgg                                                                                                                                     <  1:745575/148‑1 (MQ=255)
     aaGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATgg                                                                                                                                     <  1:1167898/148‑1 (MQ=255)
      aGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGGTATATAGGGTTGATGGt                                                                                                                                    <  2:54018/148‑1 (MQ=255)
         ttCTAAACGTCAAAGGTCTTCAGCACATAAAAATACAAGAACAATCATTGTGACATTTAACGATTTAAAGGATGTTATTAATTATGCATATCATAGCAGTCCAATAACTACAGAGTTCAAGGATTTGTTATATAGGGTTGATGGAACt                                                                                                                                 >  2:490727/1‑148 (MQ=255)
         ttCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACt                                                                                                                                 >  1:194464/1‑148 (MQ=255)
             aaaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttatt                                                                                                                             <  1:388688/148‑1 (MQ=255)
              aaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttatta                                                                                                                            >  1:1364009/1‑148 (MQ=255)
              aaCGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGAATTGTTATATAGGGTTGATGGTACttatta                                                                                                                            >  1:752252/1‑148 (MQ=255)
                cGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGACATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCGATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttattatt                                                                                                                          <  2:992365/148‑1 (MQ=255)
                cGTCAAAAGCCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACttattatt                                                                                                                          <  2:1105878/148‑1 (MQ=255)
                       aGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGt                                                                                                                   <  1:665300/148‑1 (MQ=255)
                       aGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGt                                                                                                                   >  1:58242/1‑148 (MQ=255)
                             cAGCACGTAAAAATACAAGAACAATGATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCTAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATAttt                                                                                                             <  1:866032/148‑1 (MQ=255)
                             cAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATAttt                                                                                                             <  1:193482/148‑1 (MQ=255)
                              aGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGGTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATAtttt                                                                                                            <  2:667368/148‑1 (MQ=255)
                                cACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGa                                                                                                          <  2:758472/148‑1 (MQ=255)
                                cACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGa                                                                                                          >  1:757207/1‑148 (MQ=255)
                                         aTACAAGAACAATCATTGTGAAATTTAACGATTTAGAACATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTAGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGt                                                                                                 >  2:936158/1‑148 (MQ=255)
                                          tACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGtt                                                                                                <  1:937019/148‑1 (MQ=255)
                                             aaGAACCATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGAt                                                                                             <  1:1147329/148‑1 (MQ=255)
                                             aaGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTTAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGAt                                                                                             <  1:1280000/148‑1 (MQ=255)
                                             aaGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGAt                                                                                             <  1:1412918/148‑1 (MQ=255)
                                               gaagaaTCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCa                                                                                           <  2:413984/148‑1 (MQ=255)
                                               gAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCa                                                                                           >  1:529977/1‑148 (MQ=255)
                                                 aCAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAAGCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaag                                                                                         <  2:1008337/148‑1 (MQ=255)
                                                   aaTCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaagaa                                                                                       <  1:886244/148‑1 (MQ=255)
                                                   aaTCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaagaa                                                                                       >  1:1113514/1‑148 (MQ=255)
                                                   aaTCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGATTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGGTCaagaa                                                                                       >  1:866521/1‑148 (MQ=255)
                                                    aTCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTAGGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCaagaag                                                                                      <  2:1096366/148‑1 (MQ=255)
                                                     tcgttGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGt                                                                                     <  2:1184549/145‑1 (MQ=255)
                                                      cATTGTGAAATTTAACGATTTGGAATATGTTATTAATTAGTCATATCTGATCAATCCTATCTCTATGGAGTTTGCAGTTTCGCTATATAGGGATGTTGTTAGTGATTATTATCCTGTCTTTTTTGTTTTCCATTTTTATCAAGAAGTc                                                                                    <  2:443104/148‑1 (MQ=255)
                                                        ttGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCAt                                                                                  <  2:182750/148‑1 (MQ=255)
                                                        ttGTGAAATGTAACAATTTATAAAATGTTATTAATTATCCATTTTATAGCAATCCAATAACTACAGAGTTTGAAGATTTCTTATATAGGGTTGATGGTACTTATTATTATGCTTTATATTTTGATAGTCATGTTGATCAAGAAGTCAt                                                                                  <  2:328536/148‑1 (MQ=255)
                                                          gtgCAATTTAACGATTTAGAAGATGTTATTAATTCTGACTCTTGTTGGCCATC‑‑AGATCTACCGGCGTGGGGACATTTGTTCTATAGGGTGCATGTGACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTa                                                                                <  2:641122/148‑1 (MQ=255)
                                                          gtgAAATTTAACGATGTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTGTTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTa                                                                                <  1:295523/148‑1 (MQ=255)
                                                            gAAATTTAACGATTTAGAAGGTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGGTGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAAt                                                                              <  1:933398/148‑1 (MQ=255)
                                                            gAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCCTAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAAt                                                                              <  1:765546/148‑1 (MQ=255)
                                                            gAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAAt                                                                              >  1:1148793/1‑148 (MQ=255)
                                                            gAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGAACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAAt                                                                              >  2:609259/1‑148 (MQ=255)
                                                               aTTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGAt                                                                           <  1:1212094/148‑1 (MQ=255)
                                                                      gATTTAGAAGATGTTATTAATTATGCATACCATAGGGATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACa                                                                    <  1:189582/148‑1 (MQ=255)
                                                                       aTTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAg                                                                   <  2:830982/148‑1 (MQ=255)
                                                                           agaagaTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCaa                                                               >  1:162886/1‑148 (MQ=255)
                                                                               gaTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGc                                                           <  1:95141/148‑1 (MQ=255)
                                                                                aTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt                                                          <  1:149056/148‑1 (MQ=255)
                                                                                aTGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCt                                                          <  1:125788/148‑1 (MQ=255)
                                                                                    tattaATTATGCATATCATAGCAATCCAATCACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGaa                                                      >  2:7188/1‑148 (MQ=255)
                                                                                    tattaATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGaa                                                      <  2:714350/148‑1 (MQ=255)
                                                                                       taattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCCATTGACTGAAttt                                                   >  2:1160365/1‑148 (MQ=255)
                                                                                       taattaTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAAttt                                                   >  1:833393/1‑148 (MQ=255)
                                                                                           taTGCATATCAAAACAAACCAATAACTACAGAGTTTGAAGATTTGTAATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCtt                                               <  1:583415/148‑1 (MQ=255)
                                                                                            aTGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTa                                              >  1:1157617/1‑148 (MQ=255)
                                                                                             tGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTAt                                             >  2:128128/1‑148 (MQ=255)
                                                                                             tGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTAt                                             >  2:1274973/1‑148 (MQ=255)
                                                                                             tGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTAt                                             >  2:429970/1‑148 (MQ=255)
                                                                                             tGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTAt                                             >  2:971189/1‑148 (MQ=255)
                                                                                             tGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTAt                                             >  2:62942/1‑148 (MQ=255)
                                                                                             tGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGCATCATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGAGCAAGAAATCATTAGTGATGGTTAGAGTCAAGTGCGTGAATTTGCTTaa                                             >  2:1305721/1‑147 (MQ=255)
                                                                                              gCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTAGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATc                                            <  2:753404/148‑1 (MQ=255)
                                                                                              gCACATCATAGCAATCCAAAAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTTTATATTTTGATAGTCAGGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATc                                            <  1:267578/148‑1 (MQ=255)
                                                                                               gaatatCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATc                                            <  1:1184851/146‑1 (MQ=255)
                                                                                                 atatCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCa                                          <  1:577341/148‑1 (MQ=255)
                                                                                                    tCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAaca                                       >  1:532220/1‑148 (MQ=255)
                                                                                                    tCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAaca                                       >  1:1410368/1‑148 (MQ=255)
                                                                                                    tCATAGCAATCCAATAAATACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAaca                                       >  1:204344/1‑148 (MQ=255)
                                                                                                         gCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAacagacag                                  >  2:1213355/1‑148 (MQ=255)
                                                                                                         gCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAacagacag                                  >  2:1127316/1‑148 (MQ=255)
                                                                                                          cAATCCAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTACATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAAcagacaga                                 >  2:944831/1‑148 (MQ=255)
                                                                                                               cAATAACTACTGTGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaacaga                            <  1:1225403/148‑1 (MQ=255)
                                                                                                               cAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaacaga                            <  2:1364009/148‑1 (MQ=255)
                                                                                                               cAATAACTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGacagaacaga                            <  2:752252/148‑1 (MQ=255)
                                                                                                                   aaCTACAGAGTTTGAAGATTTGTTATATAGGGTTGATTGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGtt                        <  2:470028/148‑1 (MQ=255)
                                                                                                                   aaCTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGtt                        <  1:309930/148‑1 (MQ=255)
                                                                                                                    aCTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGttt                       >  1:504562/1‑148 (MQ=255)
                                                                                                                    aCTACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGttt                       >  1:1069460/1‑148 (MQ=255)
                                                                                                                      tACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCACCAGACAGAACAGAAGtttat                     >  2:569406/1‑148 (MQ=255)
                                                                                                                      tACAGAGTTTGAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGtttat                     <  1:311199/148‑1 (MQ=255)
                                                                                                                           gagTTTGAAGATGTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTAGGTTTTGATAGTCATGTTGATCAAGATGTCATTAATGATAGTTACAGTCAATTGCGTGAATTTGCTTATCCAGCAGACAGAACAGAAGTTTAGTTaa                 <  2:411241/148‑1 (MQ=255)
                                                                                                                                 gAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCGTTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACt           >  1:539877/1‑148 (MQ=255)
                                                                                                                                 gAAGATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATATTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTCTATTTAAATGACt           >  1:1006899/1‑148 (MQ=255)
                                                                                                                                    gATTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATg        >  1:546442/1‑148 (MQ=255)
                                                                                                                                     aTTTGTTATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGc       >  2:157843/1‑148 (MQ=255)
                                                                                                                                      ttagTTATATAGGGTTGATGGTACTTATTATTATGCGGTATAAGTTGATAGTCATGTTGAGCAAGAAGTCATTAGTGCCAGTTACATTCAACTGATTGAAATTCCTTATCCAACAGAAAGAACAGAAGTTTATTTAACTGACTATGCt      <  2:684545/145‑1 (MQ=255)
                                                                                                                                          ttATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGCTaaaa  >  2:742895/1‑148 (MQ=255)
                                                                                                                                          ttATATAGGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGCTaaaa  >  1:1388125/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
AGCTCAAGGTTCTAAACGTCAAAAGTCTTCAGCACGTAAAAATACAAGAACAATCATTGTGAAATTTAACGATTTAGAAGATGTTATTAATTATGCATATCATAGCAATCCAATAACTACAGAGTTTGAAGATTTGTTATATATGGTTGATGGTACTTATTATTATGCTGTATATTTTGATAGTCATGTTGATCAAGAAGTCATTAATGATAGTTACAGTCAATTGCTTGAATTTGCTTATCCAACAGACAGAACAGAAGTTTATTTAAATGACTATGCTAAAA  >  CP000730/1002057‑1002340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: