Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,232,148 G→T 100% G18V (GGC→GTC)  prkC → non‑specific serine/threonine protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,232,1480GT98.1% 105.5 / ‑5.8 39G18V (GGC→GTC) prkCnon‑specific serine/threonine protein kinase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (21/16);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACTCATCA  >  CP000730/1232011‑1232284
                                                                                                                                         |                                                                                                                                         
gTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAGAGATAGCGTTCCTGTCATATACGATACGAGTGAAGGTGATAAAGTATGATATGTTCAATAATAAATGAAGGATATGAAATTGTAGATAAGCTTGGcgtcgg                                                                                                                                        >  2:335756/1‑141 (MQ=255)
gTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGcgtcgg                                                                                                                                        >  2:165311/1‑141 (MQ=255)
   aTCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTgg                                                                                                                                     >  1:545627/1‑141 (MQ=255)
        ttAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTACATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCCTGGCGTCGGTGGCATGa                                                                                                                                >  2:33241/1‑141 (MQ=255)
        ttAATGCAATTGGCATTAGATAAAAAGTCTAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGAATGa                                                                                                                                >  2:149494/1‑141 (MQ=255)
                aTTGGCATTAGATAACCATTCGAAAGATAACGCTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCAGGAGTAACGtt                                                                                                                        >  1:199591/1‑141 (MQ=255)
                aTTGGCATGAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGtt                                                                                                                        >  1:443965/1‑141 (MQ=255)
                    gCATTAGATAACCATTCGAAAGATAACGTTACTTTGATACTCGCGGCTATTGAAGGTGATGAAGTATGATAGGTTAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGGGTGGGTGGCATGGGTACCGTTTAtt                                                                                                                    >  2:421505/1‑140 (MQ=255)
                       ttAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTg                                                                                                                 >  2:438500/1‑141 (MQ=255)
                                 aTTCGAAAGAAAACGATACTTTCATACTCGCGGCTATTGAAGGTGAAGTCGGATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAgatac                                                                                                       <  1:226247/141‑1 (MQ=255)
                                  ttCGAAAGATAACGTTTCTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAgatacg                                                                                                      <  2:332760/141‑1 (MQ=255)
                                   tCGAAAAATAACGTTACTTTCATACTCGCGGCTATTGAAGTTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAgatacga                                                                                                     <  2:334694/141‑1 (MQ=255)
                                       aaGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACt                                                                                                 >  2:441216/1‑141 (MQ=255)
                                             aCGTTACTTTAAGACTCGCAGGTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACAt                                                                                           <  2:112313/141‑1 (MQ=255)
                                               gTTACTTTCATTTTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATTATAAATGAACGTTATAAAATTGTAGATAAGCTTGGCGCCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTa                                                                                         <  2:594330/141‑1 (MQ=255)
                                               gTTACTTTCATACTCGCGGCTATTGAAGGTGATTAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAAGATTa                                                                                         <  1:473095/141‑1 (MQ=255)
                                               gTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAACTCCTGGATTAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATCCTTAACATTa                                                                                         <  1:504316/141‑1 (MQ=255)
                                                     ttCATACTGGCGGCTATTGAAGGTGATAAAGTATGATCGGTAAAATCATAAATGAGCGATAAAAGAATGTAGATAAGCTTGGCGTCGGGGGCATGAGTAACGTTTAACTTGCTGAAGCTACGCTACTTAAGATTAAAGATg                                                                                   >  2:575660/1‑141 (MQ=255)
                                                     ttCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTg                                                                                   >  2:116662/1‑141 (MQ=255)
                                                             cgcgGCTATTGAAGGTGATAAAGTATCATAGGTAAAATAATAAATGAACGATAAAAAATTGTAGATAAGCTTGGGGTCGGAGGAATGAGTACCGTTTATCTTGCTAAAGATACGAGACTTACCATTAATGCTACAATTaat                                                                           >  2:431990/1‑140 (MQ=255)
                                                               cgGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGGTGCAATTAAGGc                                                                         >  1:555270/1‑141 (MQ=255)
                                                                     ttGAAGGTGATAAAGTATGATCGTTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGAtttt                                                                   <  1:312219/141‑1 (MQ=255)
                                                                     ttGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGCACGATATACAATTGACACTATACATGGGGGCTTGGTTGTTATTATTGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGGGAtttt                                                                   >  1:137878/1‑141 (MQ=255)
                                                                     ttGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGAtttt                                                                   <  1:126219/141‑1 (MQ=255)
                                                                     ttGAAAGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGAtttt                                                                   <  1:555051/141‑1 (MQ=255)
                                                                       aaaaaacaTAAAGACTGATAGGTAAAATAATAAATTAATGAAACAAAATTGCAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTta                                                                 <  2:32923/134‑1 (MQ=255)
                                                                           gTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCTGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATacc                                                             >  2:179677/1‑141 (MQ=255)
                                                                               tAAAGTATGATAAGTAAAAGAATAAATGAAAGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCt                                                         <  2:691545/141‑1 (MQ=255)
                                                                                aaaGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTa                                                        >  1:430270/1‑141 (MQ=255)
                                                                                aaaGTATGATAGGTAAAATAATAAAGGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTa                                                        <  2:82904/141‑1 (MQ=255)
                                                                                   gTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTagag                                                     <  1:441216/141‑1 (MQ=255)
                                                                                         tatgtcacataataAATGTCCGAATTAAAATTGGAGATAAGCTGGGCGTCGGTGGCATGAGTACAGTGTAGCTTGCTGCAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAaag                                               <  1:161436/133‑1 (MQ=255)
                                                                                          aGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATCAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAaaga                                              <  1:368057/141‑1 (MQ=255)
                                                                                                aataatAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGaaaa                                        >  2:160289/1‑140 (MQ=255)
                                                                                                aataatAAATGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAAAAGAAAc                                        >  2:277572/1‑141 (MQ=255)
                                                                                                        aTGAACGATATAAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAAc                                >  1:673885/1‑141 (MQ=255)
                                                                                                                  taAAATTGTAGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCCATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAAGGTTTAGAACg                     >  2:172465/1‑141 (MQ=255)
                                                                                                                           aGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTTAACGAGAAGTACa            >  2:97415/1‑141 (MQ=255)
                                                                                                                           aGATAAGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACa            <  1:152051/141‑1 (MQ=255)
                                                                                                                                aGCTTGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACt       >  1:99365/1‑141 (MQ=255)
                                                                                                                                   ttGGCGTCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACtcat    >  1:666407/1‑141 (MQ=255)
                                                                                                                                     ggcgtcGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTAGAACGGAAGTACATAACtcataa  >  2:105890/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                         
GTGATCAATTAATGCAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAACATTAAAGTTGCAATTAAGGCGATTTTTATACCACCTAGAGAAAAAGAAGAAACATTAAAACGTTTTGAACGAGAAGTACATAACTCATCA  >  CP000730/1232011‑1232284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: